Wisconsin-Madison/2 June 2009
From 2009.igem.org
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June 2, 2009
Other: Floors in Lab have new finish… No more leaking Autoclave juices pouring on the summer lab in the basement
Ex 6: Inducible Cultures
Inoculated four 250 mL beakers of 100 mL salt-free liquid LB
Samp1: 100uL, amp 100uL Cm, 73.5 uL Tet : Triple
Samp2: 100uL, amp 100uL Cm, 73.5 uL Tet : Triple
Samp3: 100uL, amp 100uL Cm, 73.5 uL Tet : Triple
Samp4: 100uL, amp 100uL Cm : Empty Vectors pBad33 and pTrc99
Ex 5: Results from plating of competent cells from previous day:
No freezing produced colonies. Freezing for 1 hour produced no colonies.
Results: Have triple transformation with Combination of interest (MevT, MBI, NudF)
For using comp cells: either fresh of frozen overnight
Primer: Designed Primers for NudF
Other: Worked on Wiki Pages