Wisconsin-Madison/2 June 2009

From 2009.igem.org

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June 2, 2009

Other: Floors in Lab have new finish… No more leaking Autoclave juices pouring on the summer lab in the basement


Ex 6: Inducible Cultures

Inoculated four 250 mL beakers of 100 mL salt-free liquid LB

Samp1: 100uL, amp 100uL Cm, 73.5 uL Tet : Triple

Samp2: 100uL, amp 100uL Cm, 73.5 uL Tet : Triple

Samp3: 100uL, amp 100uL Cm, 73.5 uL Tet : Triple

Samp4: 100uL, amp 100uL Cm : Empty Vectors pBad33 and pTrc99


Ex 5: Results from plating of competent cells from previous day:

No freezing produced colonies. Freezing for 1 hour produced no colonies.

Plate 0602.jpg

Plate 0602-2.jpg

Results: Have triple transformation with Combination of interest (MevT, MBI, NudF)

For using comp cells: either fresh of frozen overnight


Primer: Designed Primers for NudF


Other: Worked on Wiki Pages