Wisconsin-Madison/3 June 2009
From 2009.igem.org
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June 3, 2009
Other: 1. Made freezer stock of triple transformation and double transformation
2. Nate attempts to fix autoclave and went to lab and made new pipe
3. Prepare for advisor meeting tomorrow
4. Received Streptomycin in Mail
Primer: NudF Primer finalized
Designing yHfR and ProU Primers
Ex 7: Isolation of Genomic from gram negative DNA from E.Coli K12 using Promega kit, Ptotocol for later isolation of Pro U operon and promoter
Results: Spec DNA: 337mg/ml
- made freezer stocks of pNudF, pMBI, pMevT and pBAD33, pTrc99A transformed e coli - NP
- made a freezer stock of K12 and extracted its genomic DNA - NP