Wisconsin-Madison/4 June 2009
From 2009.igem.org
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June 4, 2009
Other: Meeting
Worked on Website
Ex 6 Results: No good, past optimum optical density
Need more samples
Made more inducible cultures: (IPTG)
1. Sample 1: 100ml LB,50uL Amp, 73.5uL Cm , 100uL Tet : Triple Transformation
Induced with +50uL IPTG 1.5 g Arabinos
2. Sample: 100ml LB 50uL Amp 73.5uL Cm , 100uL Tet : Triple Transformation
Let sit for overnight
3. Control: 100ml LB, 50uL Amp, 73.5 Cm : Empty Plasmids 33+99
Ex 5: Plated Triple on Amp, Cm, Tet Plate from Freezer for Test
Other:
Made Antibiotics from Streptomycin
Made plates with Streptomycin
Nate got new valve and pipe for autoclave and it works now
- induced a pNudF, pMBI, pMevT culture with IPTG and arabinose (unsuccessful) - NP