Wisconsin-Madison/5 July 2009
From 2009.igem.org
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July 5, 2009
Ex 16: Cloning
Gel Extraction:
Made 2: 40 mL of 1% Gel – 0.4 g (8 wells)
3 uL Dye / 4 uL Sample
20 uL 1)NudF1, 2)NudF1, 3)NudF2, 4)NudF2, 5) Lac1, 6)1kb Ladder, 7)Lac2, 8)Lac2
Attempted gel extraction but couldn't see the band
Ex 19: Parts from the Registry
Inoculate:
1. lac
2. lac+GFP
3. RFP
Primer: Design for GsSDMT, new ProU(including ProV), MevT, MetK, pMBI