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Wisconsin-Madison/5 June 2009
From 2009.igem.org
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June 5, 2009
Ex 5: Results Triple Freezer Test is good
Ex 6: Sample 1: Results: Nothing from visible testing with match (video)
Isopentenol is flammable and we expect a small poof or flame from the sample
Sample 2: Placed 2.5 ml of sample to into fresh 100ml LB, 50uL Amp, 73.5uL Cm, 100uL Tet
OD 600
1. 0.078
2. 0.067
3. 0.077
4. 0.112
5. 0.146
6. 0.208 Induced with 1.02g Arabinose
7. 0.406 Induced with 100uL IPTG
LET SIT OVER WEEKEND
Ex 7 results: Ran .7% Gel for verification of DNA, good
Primer: Spinach cDNA Primer Design
Other: research
Ex 8: Obtaining Additional Promotors: pSB1A3-1, BBa_J33210, rMMBRraA
1. Plated High copy BioBrick assembly plasmid
[[1]]
pSB1A3-1 is a high copy number plasmid carrying ampicillin resistance.
Well K1 of 2009 Spring Kit Plate 1 --- Plasmid pSB1A3 with part BBa_J04450
2. Broad host range BioBrick plasmid
[[2]]
BBa_J33210 - low to medium copy number, derived from pRL1383a, Streptomycin
Spring 2009 Distribution, 2009 Kit Plate 3, Well 17B
3. Brian’s RFS1010 derived Plasmid - rMMBRraA
- transformed competent DH10B cells with pRL1383a and pSB1A3 (successful) - NP
