Wisconsin-Madison/9 July 2009
From 2009.igem.org
Calendar
|
|
|
|
|
|
|
July 9, 2009
Other:
Advisor Meeting – Brian
Research
ProU, ProV cutting
Ex 20: New Cloning Methods
J. MiniPrep:
1. NudF: Amp
2. Choline: Amp
3. Backbone P1: Amp
K. Digest:
1. NudF – (560+2157=) 2717 bp
2. Choline – (2000+2157=) 4157 bp
3. Backbone P1 – 2157+1069 bp
4. (pVP68K): gsSDMT (Pst1) – (931+5212=) 6143 pb
Cut with (---), Ran on 1% Gel
Left to right:
Uncut: NudF 2B, Choline1, Backbone 1, GSSDMT1, Ladder
Cut: NudF 1A, 1B, 2A, 2B, choline 1,2, Backbone 1, 2, GSSDMT1, 2
Inoculated:
DH1: Blank (no resistance)
Ex 19: Parts from Registry
Transformation plate 1 well O22 into DH10B
Ex 10: Cyanobacteria
natural transformation test on 7002 with 1mL culture and 10uL pMMBRraA, allowed to incubate for 24h
added 9mL Media A to 7/8 nat trans test cultures and 7.35uL or 10uL of CM, allowed to incubate for 48h
made freezer stock of 7002 in 25% glycerol – NP
Primer Design:
1st Antiporter
2nd Antiporter
New ProU
Ex 18: pTaq