Wisconsin-Madison/15 October 2009

October 15, 2009
Cloning

- Digestion

- PCR Clean up

- Ran Gel

- Found concentrations

- Ligations

PhotoPromoter: pHawaii

pHawaii - vector only

ProtoPromoter: pSB1A2

pSB1A2 - vector only

ProU:RBS+RFP

RBS+RFP - vector only

Thermocycler -> overnight

Inoculations

- 5 confirmed BioBricks

- New Parts

- Genes in pBAD for testing in Minimal Media

Sending DNA

- Prepared DNA in single PCR Tubes