User:DavidC/11 October 2009

DNA extraction:
Extraction of p53 from E.coli DH5alpha using a maxiprep (macherey nagel) (2 times).

Cell culture
Growth of E.coli into 5mL of LB medium with 5µL of the correct antibiotic (ampicillin 50mg/mL, kanamycin 50mg/mL or tretracycline 12,5mg/mL).

Samples: BBa_B0030; BBa_C0012; BBa_B0014; BBa_ B0030 + BBa_C0012 + BBa_B0014.

Test of BBa_B0030 + BBa_C0012 + BBa_B0014
85 Volt, 15 minutes. 105 Volt, 40 minutes. Ladder fermentas 1 Kb.

Samples: BBa_B0030 + BBa_C0012 + BBa_B0014.