Template:Team:KULeuven/16 September 2009/VanillinProduction


 * Restrictions from yesterday were put on gel (COMT2, 3, SAMS, Promotor)
 * SAMS and promotor( J23110??) were gel purified
 * Ligation of SAMS + promotor
 * 28 &mu;l of SAMS and 11 &mu;l from promotor, almost 4:1 ratio (although maybe closer to 3:1)
 * Electroporation of EFT ligation from yesterday on Km plates (Ter vector has Km resistance, so a closing of EF vector on its own doesn't grow!)
 * Miniprep
 * The liquid culture of SAMS was used for the glycerol stock and send for sequencing