Team:TorontoMaRSDiscovery/26 August 2009

=August 26, 2009=
 * 1) 4+5 plates showed 2 big colonies and 1 small colony
 * 2) Started log phase for 4+5 (1), (2) (did not stock)
 * 3) 3+2 overnights did not grow
 * 4) *They are not C-resistant?
 * 5) *We have to redo the 3+2 ligations
 * 6) Miniprepped Enc A, B, C, BB3 and BB4
 * 7) Digested miniprepped samples and also BB2, 1+2 plasmid for 2 hours
 * 8) Ran digests on a gel
 * 9) *1ug of plasmid was digested for Enc A, B, C, BB2, BB3, BB4; and 1/5 was loaded on to gel (10ul of digest)
 * 10) 30x37.4=1122ng of plasmid was digested for 1+2; and 16.5ul was loaded (1122x16.5/50=370.26ng)
 * 11) Miniprepped log phase of 4+5 (1), (2)