Team:Newcastle/IntroductoryLabwork/1 July 2009

Introductory Lab Session: 1st July 2009
'''Today's introductory lab session saw the team progress with the next steps in the making competent cells procedure (i.e. steps 2 and 3 in the 'Pre-culture section). The team also looked at the future steps involved in the protocol and prepared solutions and equipment accordingly. This lab session (as well as the previous one) taught the team the importance of planning, organisation and time management.

Things needed for today's session
Things we need for today include:
 * LB
 * Containers
 * Sterile tubes
 * Space in orbital shaking incubator for tonight

Progressing with making competent cells

 * We plated out single cultures from yesterday's plates for a practice.
 * We inoculated 2ml of LB with single cultures from yesterday's plates and put them in the shaking incubator.
 * We made up some 50% glycerol and it was sent to be autoclaved.

Considerations

 * How much equipment (e.g. tubes) and solutions have we used today? Is there enough for Thursday's lab session?

Things we need for tomorrow (Thursday):
General 'Culture' Step (from making competent cells protocol) 'Harvest cells' Step (from making competent cells protocol)
 * 1) Sterile tubes
 * 2) Pipette tips
 * 3) Solutions
 * 1) Sterile flasks for culture (500ml)
 * 2) Spectrophotometer and cuvettes (where)
 * 3) Arrange space in shaking incubator
 * 1) Centrifuge/ centrifuge vial (200ml?)
 * 2) Ice bucket and ice
 * 3) Glycerol
 * 4) Sterile microfuge tubes
 * 5) Liquid nitrogen