Team:PKU Beijing/Notebook/AND Gate 1/Input/AraC

Notebook > AND Gate 1 > Input > Arabinose Sensor

2009.7.23
10:30 Double-enzyme digestion: 1-23L, 1-2M+AraC

16:50 Gel purification

19:00 Ligation: Insert: 1-2M+AraC Vector: 1-23L

23:00 Double-enzyme digestion

2009.7.24
Gel purification

2009.7.27
19:00 Ligation: Insert: 1-2M+AraC+1-23L Vector: Pcat

23:00 Transformation

2009.7.28
11:00 PCR: Pcat+1-2M+AraC+1-23L

23:00 Mini-prep and store strains: 1, 2, 4, 5, 6, 7 BioSpec(×50 μg/mL):

00:30 Reverse PCR: Pcat+1-2M+AraC+1-23L

2009.7.29
12:30 Gel purification BioSpec(×50 μg/mL): 0.1964

14:00 Double-enzyme digestion: Reverse PCR fragment and plasmid of Pcat+1-2M+AraC+1-23L

20:30 Gel purification

22:00 Double-enzyme digestion: 1-1F (Front vector)

2009.7.30
13:30 Gel purification: 1-1F

15:00 Ligation: Insert: Pcat+1-2M+AraC+1-23L Vector:1-23L

21:00 Transformation: ligation product

2009.7.31
10:30 PCR

16:00 PCR

23:00 Mini-prep

2009.8.1
12:00 Transformation

2009.8.2
11:30 Pouring plates: LB+Amp ×30, LB+Kan ×50

15:30 Mini-prep: pla1~5, pcr1~4 Gel electrophoresis

17:00 Double-enzyme digestion (front insert) Plasmid: pla1, pla2, pcr1, pcr3

22:30 Gel purification (pcr1, pla1, pla2)