Team:Groningen/Notebook/18 September 2009

GVP Cluster
Planning


 * → work out the wiki page for GVP
 * made a layout
 * still have to read all articles
 * modeling will stay as it is, has been done by modeling people
 * → ✅ make a doodle for presentation planning (1-19 oct.)
 * → ✅ media attention
 * mail to UK, Ing., St. Gen.
 * facebook account with link to twitter
 * ethics survey link on facebook and twitter
 * → ✅ place an ethics survey link on twitter


 * → ✅ clone pArsR-GVP into pSB2K3
 * still need to make glycerol stock
 * → clone repeat out of GVP cluster
 * → make glycerol stocks of constructs
 * → enter info on part registry

Plates


 * → The plates showed the expected amount of colonies, and also red in the case of the positive plates. The eight colonies on the negative plate can be taken into acount when plating transformed cells.


 * → The plate with stripes of pArsR-GVP (pSB2K3) preculture showed growth and some single colonies.


 * → All plates were stored in the fridge for further use on monday.

Cultures

The overnight cultures with LB-amp100 medium of colonies E.coli TOP10 with pNL29 in pBlueScript_II_KS_(+) showed no growth.


 * → The plate used for culture inoculation was very (more than a month) old, and was probably no good anymore. The new transformed E.coli TOP10 with original pNL29 plasmid will replace all excisting stocks/plates.