ULB/9 July 2009



 iGEM Team:ULB-Brussels Wiki  

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Université Libre de Bruxelles

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 Home Team Project</a></li> Parts</a></li> Safety</a></li> Notebook</a></li> Sponsors</a></li> </ul> <ul>

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{| align="right"

Choice of bacteria strains :


 * Caulobacter crescentus (strain CB15N)
 * E. Coli (strain K12 or DG1)

Constructions
Construction 1

As Caulobacter crescentus genes of interest have E. coli homologs, we’ll first transfer HfsG et HfsH genes through the assembly standard 21 and we hope E. Coli will be able to produce the adhesive material.

Forward and reverse primers are designed.

hfsG and hfsH share a single nucleotide  HfsG : ---TGA  ;  HfsH : ATG--- ==> a blunt-end ligation(?) is possible.

Terminator chosen : BBa_B0015