User:DavidC/21 August 2009

PCR
PCR of adenovirus 5 penton base from plasmid coding for the virus.

94°C, 2 minute. (94°C, 1 minute. 55°C, 1 minutes. 72°C, 3 minutes.) X 5 72°C, 5 minutes. (94°C, 1 minute. 66°C, 1 minutes. 72°C, 3 minutes.) X 30

DNA electrophoresis
85 Volt, 15 minutes. 105 Volt, 40 minutes. Ladder fermentas 1 Kb. sample: adenovirus penton base (ADV5pb)



No amplification of the adenovirus 5 penton base, we have mismatch on the second trial.

Lambda phage extraction
Lambda phage extraction. 2 dishes, at 12 and 24 hours of incubation, dilution = 1/10 and 1/10 000.

Growth of lambda phages for extraction. 5 dishes with E.coli infected by Lambda phages, dilution = 1/10.