Template:Team:KULeuven/13 August 2009/VanillinProduction


 * 1) Plated COMT (+RBS) from the registry
 * 2) * Growing overnight in 37°C
 * 3) Plated ech
 * 4) * From newly made liquid culture
 * 5) Miniprep of ech and sam8
 * 6) * Nanodrop results:
 * 7) Restriction digest on ech and sam8
 * 8) Gel electrophoresis on ech and sam8
 * 9) New electrocompetent cells were used for the electroporation with the old ligations
 * 10) * Lig A -> sam5 + sam8
 * 11) * Lig C -> ech + fcs
 * 12) * Note: there was no spark
 * 13) Plating of the electroporated cells
 * 14) * 200 &mu;l per plate twice
 * 15) * 600 &mu;l left of A and C