Team:TUDelft/18 August 2009

=18 August 2009=

Sriram
Checking the yesterday's gel (shown below) I found that since the ladder didn't run well we couldn't interpret the size properly. hence I decided to do the whole assembly again. Or else we need to follow the back up plan for negative cascade alone created by Daniel. So today to start everything again I made cultures for all the primary biobricks to start the assembly all over again. We also have one problem the restriction enzyme SpeI is getting over. So it was ordered today.

Calin
Yesterdays 20 well 1% gel:



Yesterdays 26 well 2% gel:



For the trbK conjugation test 24 plates were made:

For the oriTR conjugation test 51 plates were made:

Tim Weenink
Results of the 24h sequencing are in:


 * !BPEA1 (R) correct in reliable part of sequencing file
 * !BCEC1 (R) correct in reliable part of sequencing file
 * !CCEA1 (F) perfect
 * !CCEC2 (F) perfect
 * !SPEA1 (F) Assebly with !A and BBa_K142202. !A is not supposed to be there
 * !SPEA1 (R) Assebly with !A and BBa_K142202. !A is not supposed to be there
 * !SPEC1 (F) Failed (as expected)
 * !SPEC1 (R) Failed (as expected)
 * *I6F (F) Perfect
 * *S3 (F) Is not BBa_K142205, but in fact BBa_K142203
 * *S3 (R) Is not BBa_K142205, but in fact BBa_K142203

Daniel
Growth, miniprep: