Wisconsin-Madison/12 July 2009

July 12, 2009
Cut lac with E and Spec2 Buffer EcorI DIGEST

RFP and RBS Eco Xba1

PCR Clean UP

Spec Ligation

TRANSFORM Trip __> DH1 Anp test

Lac RFP (RBS ---dh10b

Lac 50

Rfp rbs 90 ug/ml

Speed Vac

Ligation 2.5 RFP 5.5 Lac 2 buffer 1 ligase

- added 100mL media A to 7/9 natural transformation test cultures and 100uL CM - NP