Team:IPN-UNAM-Mexico/Notebook/August 10 2009

    





     <a href="http://2009.igem.org/Team:IPN-UNAM-Mexico" onMouseOut="MM_swapImgRestore" onMouseOver="MM_swapImage('HOME',,'http://2009.igem.org/wiki/images/f/f5/Home.jpg',1)"><img src="http://2009.igem.org/wiki/images/e/ec/Home2mx.jpg" alt="Home" name="HOME" width="113" height="32" border="0"></a><a href="http://2009.igem.org/Team:IPN-UNAM-Mexico/Project" onMouseOut="MM_swapImgRestore" onMouseOver="MM_swapImage('PROJECT',,'http://2009.igem.org/wiki/images/c/c8/Project2MX.jpg',1)"><img src="http://2009.igem.org/wiki/images/5/5e/ProjectMX.jpg" alt="The Project" name="PROJECT" width="113" height="32" hspace="1" border="0"></a><a href="http://2009.igem.org/Team:IPN-UNAM-Mexico/Team" onMouseOut="MM_swapImgRestore" onMouseOver="MM_swapImage('TEAM',,'http://2009.igem.org/wiki/images/4/46/TeamMX.jpg',1)"><img src="http://2009.igem.org/wiki/images/0/05/Team2MX.jpg" alt="The Team" name="TEAM" width="113" height="32" hspace="1" border="0"></a><a href="http://2009.igem.org/Team:IPN-UNAM-Mexico/Modeling" onMouseOut="MM_swapImgRestore" onMouseOver="MM_swapImage('MODELING',,'http://2009.igem.org/wiki/images/6/6c/ModelingMX.jpg',1)"><img src="http://2009.igem.org/wiki/images/a/a1/Modeling2MX.jpg" alt="Modeling" name="MODELING" width="113" height="32" hspace="1" border="0"></a><a href="http://2009.igem.org/Team:IPN-UNAM-Mexico/Parts" onMouseOut="MM_swapImgRestore" onMouseOver="MM_swapImage('PARTS',,'http://2009.igem.org/wiki/images/6/61/PartsMX.jpg',1)"><img src="http://2009.igem.org/wiki/images/f/f0/Parts2MX.jpg" alt="Parts" name="PARTS" width="113" height="32" hspace="1" border="0"></a><a href="http://2009.igem.org/wiki/index.php?title=Team:IPN-UNAM-Mexico/Collaboration&action=edit&redlink=1" onMouseOut="MM_swapImgRestore" onMouseOver="MM_swapImage('COLLABORATION',,'http://2009.igem.org/wiki/images/8/85/CollaborationMX.jpg',1)"><img src="http://2009.igem.org/wiki/images/3/33/Collaboration2MX.jpg" alt="Collaboration" name="COLLABORATION" width="113" height="32" border="0"></a> Electrophoresis gel load: <ul> <li>3 &mu;l of colorant</li> <li>10 &mu;l of digestion</li> <li>3 &mu;l of molecular weight marker</li> </ul> Load order. Well    Plasmid            Well             Plasmid 2         16,2&rdquo;               13            3.3 3         4:23  L2            14            22.1 4         15:3  L2            15            19.2* 5         18.2*                16            24.2&rdquo; 6         2.3                                     Well                             Well 1         1kb  2 &mu;l         12            2.3 2         16.2&rdquo;             13            14.2 3         4:23  L2          14            3.3 4         15:3  L2          15            22.1 5         18.2              15            19.1*   6          15.13                                     Horizontal Gene Transfer (HGT). Acording to the model proposed to Arturo, we made a search for the candidate bioparts to make their elusion and  transformation, which got in the next way: 2 Constructions: Construction 1: Biopart BBa_K145201 J23116 + B0034 + C0040 + B0010 + B0012 Construction 2: The first option for the construction 2 is made up from two devices: BBa_J01101 and Bba_I763011. The second one consists in asking Boston for the construction BBa_I763027. We will tell Arturo about this posibility. First option: Device 1. B0040 + B0034 + C0051 + B0010 + B0012 Device 2. B0051 + B0034 + J04031 <p align="left" class="style8">LOCATION <p align="left" class="style8"> <td style="width:688px; height:416px "> <td style="width:688px; height:109px "> <td style="width:32px; height:800px ">

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