September/9 September 2009

1.colony check ( sample No.) (no. of colonies) 9               50<        24                0         29                0        30                0        31                8        32                2         9                1

2.digestion and ligation

digestion with restriction enzyme

K204024

K204029

K204030

↓ 37degree 3hr ↓ Agarose Gel Electro-phoresis No. 24, 29 , 30 , 10-D , 10-E , ladder ↓ 16 degree O/N

3.Min prep Result of Nanodrop concentration check (sample No.)     (concentration) 1-1D                27.5 10-D                85.1 10-E                117.2

4.transformation ligation sample No. 33, 34 , 35 , 36 , 37 , 38 , 39 , 40A ,40B sample No. 24, 29 , 30

5.digestion and ligation

digestion with restriction enzyme

K204041

K204042

↓ 37degree 3hr ↓ Agarose Gel Electro-phoresis No. 41, 42 , ladder ↓ 16 degree O/N

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