Virginia Commonwealth/16 July 2009

Results
Maria and Afton
 * Results of Retransformation with pSB4C5 backbone in NEB 10 beta two days after transformation


 * No growth was seen in 5mL culture taken from NEB 10 beta cell freezer stock
 * Vial may have thawed out too much after cells were taken out for the electrocompetency procedure


 * Spectrophotometry measurements after Miniprep


 * Gel Electrophoresis Results

Trentay 14:31, 16 July 2009 (UTC)

Craig and Clay
 * No growth was seen on either the kanamycin or chloramphenicol plates.
 * The sequence for the LIM1 gene was verified to be optimized for E. coli.

Tasks
Maria and Afton Trentay 20:41, 16 July 2009 (UTC)
 * Spectrophotometry
 * Digest: E0240,J06702, pSB4C5, 110, 113, 116, 0011, 0040
 * Ligate promoters with backbone pSB4C5 and reporters E0240, J06702
 * Run an electrophoresis test on promoters and reporter Eo240
 * Pick colonies of promoters from transformation 100-107 (excluding 106)
 * Transformation of NEB 10 beta to make sure new stock is electrocompetent

Craig and Clay
 * The registry will be contacted to verify the correct resistence for the limonene synthesis related parts. New DNA will also be requested.

Wetlab
Maria and Afton Trentay 20:41, 16 July 2009 (UTC)
 * Digest: E0240,J06702, pSB4C5, 110, 113, 116, 0011, 0040
 * Ligate promoters with backbone pSB4C5 and reporters E0240, J06702
 * Run an electrophoresis test on promoters and reporter Eo240