August/12 August 2009

1.before Min prep Checked the cell cultures transformed and plated out yesterday (8/10) for colony formation and made an approximate count of the number of colonies.

(plate number)-(location on plate) (no. of colonies) 1-14F                     50< 1-14H                     50< 1-23J                      100~ 1-12C                      100~ 2-18F                      10

inoculate to iquid medium(5mL + Amp 5uL or Kan 25uL)

2.Transformation to get new plasmid each 4uL(DNA), 1-2M , 1-12C : 2uL 1-2M Amp 2-8O 1-21F 2-21F 1-12A 2-8I 1-12C 2-24G 1-16G 1-18L Kan 2-11N '8/11sample 10uL RBS+2-8M(LasR) EpsE+Terminater (after 65℃, 10min)

3.LB plate LB(500uL)+Amp(2.5mL) *25 +Kan(0.5mL) *25

4.Min prep Result of Nanodrop concentration check

(sample No.) (concentration) I179005 (1-15N)            19.0 ng/uL K091101 (2-6P)              27.0 J23100 (1-18C)             21.5 C0070 (1-12H)              48.0 B0015 (1-23L)               23.7 19.4  R0040 (1-6I)                    9.3 I15009 (1-20F)                71.3 K118013 (2-18F)              19.2 C0078 (1-14H)                49.9 I1466 (1-23J)                  21.3 R0071 (1-12C)                 10.7 C0077 (1-14F)                 32

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