Illinois/30 June 2009

June 30
sRNA Library

We started creating primers for 5 new sRNA's and each of their respective target sequences:

dicF /  ftsZ dsrA /  hns rhyB /  sodB spot42/ galK rseX /  ompC and ompA gcvB /  dppa and oppa

We PCR'ed all of the genes listed above except for ompC and ompA, and we obtained bands for each of the target sequences. However, for the sRNA's, we only obtained bands for gcvB and dicF.

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