Team:Chiba/Notebook/Calendar/22 September 2009



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(21_September_2009 <|>23_September_2009)

Transformation(1)-2
Yesterday's operation is here.

We count number of colonies of each plate.
 * Today's operation

11:30

コロニーをつついて培養

pCIA3-LuxR

LacZ alpha protein generator

23:50

mCherryのコロニーができたのでつついて培養

グリストをつついて培養

LuxI

BBa_T9002

To judge character of LuxR mutants (1)-2
Yesterday's operation is here.


 * Today's operation

11:00-

We transplanted E.coli by 48 pins to NC filter and cultured it.

22:30

We transplanted E.coli, which has been cultured on NC filter, to solid medium which contains each concentration of AHL.

AHL concentration is : 0, 10, and 1000 nM

We decided this time is T=0 and observed condition of fluorescence every 30 min.


 * Mutants' Location

Pictures are here.

E. coli Painting (2)
We painted some pictures using excess culture(LuxR Mutant 1) and cultured these.

Pictures are here.

To judge character of LuxR mutants (2)-1
We poured 1 mL of LB-Amp, Cm liquid medium in 96 deep well and added glycerol stocks.

11:45-

We cultured and shook it at 37 degrees Celsius.

23:20-

We transplanted E.coli by 48 pins to NC filter and cultured it.

Examine limit of AHL generation(1)-1
23:00

We did prior culture(JW1908 glycerol stock, plac-LuxI, and 12.5 mL of LB-Amp).