Duke University/16 June 2009

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June 16, 2009

 * E: PCR with Bioplastics #2 as template and Phusion mix (done by Maggie), Gels to check PCRs

2x Phusion mix 25 ul forward primer 2.5 ul reverse primer 2.5 ul DNA template (plasmid) 1 ul H2O 19 ul 50 ul


 * R: No bands for fragments 3, Bands for fragment 4
 * C: Cut out bands of fragment 4, PCR again to obtain fragment 3


 * E: Measured concentration of DNA fragments 1 and 2
 * R: Concentrations okay
 * C: Do assembly PCR once enough of fragments 3 and 4 obtained