Team:Warsaw/Calendar-Main/1 September 2009

Making of the plac-RBS-llo-intA part Jarek

Tasks:  Isolation of plasmid DNA from liquid cultures. Digestion of DNA samples with EcoRI and PstI endonucleases. 

Cloning of the mgtc promoter into the pSB1A3 plasmid Kamil

Tasks:  Bacteria transformation 

Methods:  A fresh batch of chemocompetent bacteria was transformed with the ligation mix and incubated on agarose plates containing double dose of ampicilin 

Cloning of the cro-box into the pSB1A3 plasmid Kamil

Tasks:  Bacteria transformation</li> </ul>

Methods:  A fresh batch of chemocompetent bacteria was transformed with the ligation mix and incubated on agarose plates containing double dose of ampicilin</li> </ul>

Cloning switch 1 regulatory parts [ <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K177012">K177012</a>-PcI.RBS.LacI, <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K177033">K177033</a>-PcI.RBS.LacI.PcI.RBS.RFP.terminator, <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K177011">K177011</a>-PLacI.RBS.cI.terminator, <a href="http://partsregistry.org/Part:BBa_K177038">K177038</a>-PLacI.RBS.cI.terminator.PLacI.RBS.GFP.terminator ] into two compatible low copy number plasmids of different antibiotic resistance Ania Tasks:   </li> </ul>