Lab Aug 26 2009

9AM, Derek:

DH5alpha for the competent cells are not showing any turbidity yet. Perhaps the bucket of ice in the bath slowed things down too much.

No growth on any of last night's plates.

noon:

still not quite up to the right OD on the cells.

put in 1L of LB to the autoclave and 1L of LB with 15g of agar

3:30:

we made 500ml of Cm plates, 250ml of Amp plates, and 250ml of Km plates

made 250 ml Km broth

picked a colony of P1010Kan and put it in an LB-Kan broth for overnight

prep'ed gels for tomorrow

put cells in fridge because they hadn't quite hit OD and I had to go home.

Todo today:

oral exams

Make LB

Pour Cm Plates: Plate Pouring Protocol

Talk about sequencing - Nano

Make competent cells

Test transform

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