ULB/20 August 2009



 iGEM Team:ULB-Brussels Wiki  

  /* Design for http://2009.igem.org/Team:ULB-Brussels

Université Libre de Bruxelles

html body { width: 910px; font-family: Arial; margin: 0 auto;

}

html body div.header { margin: 0px; padding: 0px; margin-top: -21px; /* strange... */ }

/* ===== banner ===== */

/* banner = large image on the top of the page, begind the title */

html body div.header div.banner { height: 171px; background-image: url(http://2009.igem.org/wiki/images/0/0a/Logoulb1.png); background-repeat: no-repeat; }

html body div.header div.banner h1 { padding-top: 50px; text-align: center; text-decoration: none; border: none;

color: #4E7DB7; font-size: 40px; }

html body div.header ul { /* these are common to both 'menu' and 'submenu' */ margin-top: 0px; margin-bottom: 0px; list-style-type: none; list-style-image: none; }

/* ===== menu ===== */

/* menu = horizontal bar containing the main links to other sections of the wiki */

html body div.header div.menu { background-color: #3E6DA4; color: white; height: 30px; padding: 0px; }

html body div.header div.menu li { float: left; text-align: center; width: 120px; margin: 0px; margin-top: 10px; } html body div.header div.menu li :hover { color: #5F7EB4; background-color: #FFFFFF;

margin: 0px;

padding-left: 15px; padding-right: 15px; padding-top: 4px; padding-bottom: 3px; }

/* class 'selection' refers to the 'selected' menu, in order to change it's color to white */ html body div.header div.menu li a.selection { color: #5F7EB4; background-color: #FFFFFF;

margin: 0px;

padding-left: 15px; padding-right: 15px; padding-top: 4px; padding-bottom: 3px; }

html body div.header div.menu li a { color: white; text-decoration: none; }

/* ===== submenu ===== */

/* submenu = changing horizontal bar containing the main links to subsections of other sections of the wiki */

html body div.header div.submenu { /*background-color: #404040;*/ background-color: white; height: 25px; font-size: small; border-bottom-style: solid; border-bottom-width: 1px; border-bottom-color: #AAAAAA; }

html body div.header div.submenu li { float: left; width: 120px; text-align: center;

margin: 0px; margin-top: 4px; }

html body div.header div.submenu li :hover { color: #5F7EB4; background-color: #FFFFFF;

margin: 0px;

padding-left: 15px; padding-right: 15px; padding-bottom: 3px; }

html body div.header div.submenu li a { color: #5F7EB4; text-decoration: none; }

/* ===== panel ===== */

/* panel = box containing mascotte, contdown and links to sponsors */

html body div.panel { float: right; border-left-style: solid; border-left-width: 1px; border-left-color: #AAAAAA; padding-left: 10px; margin-left: 10px; margin-top: 20px; width: 250px; font-size: small; }

/* appearance of the links */ html body div.panel a { text-decoration: none; font-weight: bold; color: #3E6DA4; }

html body div.panel a:hover { color: #F5B455; }

/* == countdown == */

html body div.panel div.countdown { /*margin-top: 20px; margin-bottom: 20px; height: 30px;*/ margin: 20px; padding: 20px; }

.countdown font { padding: 20px; margin: 20px; color: #5F7EB4; }

/* == sponsors == */

html body div.panel div.sponsor { margin-bottom: 10px; min-height: 50px; }

/* appearance of the sponsor's logos */ html body div.panel div.sponsor img { width: 50px; height: 50px; border-style: none; float: left; margin-right: 7px; }

/* ===== main ===== */

html body div.main { font-size: small; padding-left: 10px; padding-right: 10px; width: 910; text-align: justify; }

html body div.main p.abstract { font-weight: bold; }

html body div.main div.welcome img { width: 626px; height: 150px; margin-top: 10px; border-style: none; }

html body div.footer { }

 Home Team Project</a></li> Parts</a></li> Safety</a></li> Notebook</a></li> Sponsors</a></li> </ul> <ul>

</ul>

<style type="text/css">

table.calendar         { margin: 0; padding: 10px; } table.calendar td      { margin: 0; padding: 2px; vertical-align: top; } table.month .heading td { padding:2px; background-color:#3E6DA4; color:#FFFFFF; text-align:center; font-size:120%; font-weight:bold; } table.month .dow td    { color:#aaa; text-align:center; font-size:110%; } table.month td.today   { background-color:#ddd; } table.month td { border: none; margin: 0; padding: 1pt 1.5pt; font-weight: bold; font-size: 12pt; text-align: right; background-color: #eee; } .day-active { color:blue } .day-empty { color:grey }
 * 1) bodyContent table.month a { background:none; padding:0 }

{| align="right"

Method
Plasmids have been extracted from our bacteria cultures.
 * ccdA forward : 2 tubes
 * promoter + RBS + RFP : 4 tubes, half of them contained red bacteria

Results
Photometry showed that there is very few extracted genetic material, except for the first ccdA tube. New cultures have then been setup for the promoter + RBS + RFP plasmid for a new extraction.

!!!Photo missing for gel migrations Results from the gel migration have not yet been interpreted.

No glycerol has been done.

Ligations: hfsG + hfsH + terminator
hfsG and hfsH are located on plasmids carrying Amp resistance from Mr. Gene. In order to be able to select plasmids with antibiotics, we will first link hfsH with our terminator because the plasmid carrying the terminator is resistant to Kanamycin (and Amp).

hfsH was digested with EcoRI and SpeI. terminator was digested with EcoRI and XbaI. The rest of the digestion follows the standard protocol. The result was dialysed and ligated.

Solid culture boxes have been created with Kanamycin.