UCL London/Protocol/Restriction Enzyme Digestion

Restriction Enzyme Digestion


 * Materials:


 * Plasmid DNA: 10.5 µL/5 µL
 * Restriction Enzymes (depend on the requirements of the parts)
 * EcoRI: 1 µL
 * PstI: 1 µL
 * SpeI: 1 µL
 * XbaI: 1 µL
 * 10× NE buffer: 1.5 µL/1 µL (lid)
 * BSA: 1 µL (lid)


 * Method:


 * 1) Add all the contents listed above to labelled eppendorfs.
 * 2) Spin for 30 sec.
 * 3) Incubation at 37°C for 1hr.
 * 4) Leave on ice before the gel electrophoresis.