Team:Washington/Notebook/Ni-column

Ni-column Set up

 * 1) Transfer 2mL NiNTA beads (Quigen) to column
 * 2) wash column with 10mL dH2O
 * 3) Equillibrate column with 10mL running buffer (usually PBS)
 * 4) To reuse column
 * 5) Wash with 12mL dH2O
 * 6) Wash with 12mL 100mM EDTA
 * 7) Wash with 12mL dH2O
 * 8) wash with 12mL 100mM Ni(SO4)
 * 9) Wash with 12mL dH2O
 * 10) Add 12mL 20% Ethanol run till ~5mL remains in column
 * 11) cap for use later