Team:Paris/27 August 2009

NoteBook
August 27th

 

Lab work
PCR on OmpA signal/g3p colony

Total volume = 25ul

2XSuper mix : 12,5 ul

Diluted bacteria : 5 ul

VF2 (10 uM) : 0,5 uL

VR (10uL) : 0,5 uL

H2O : 6,5 uL

gel purification

gel purif using promega kit

PCR

Pcr of:


 * FecR part A & B
 * Tg3p

purification on column

Purif of:
 * OmpA linker (SacI/P)  Failed (wrong size and two band)
 * FecI (X/P)                                 Failed (no  DNA ?! )
 * pBad (S/P)                               Failed (no  DNA ?! )

Transfo Jun Fos AIDA gen

Transfo A40 TolRII: topo vector

O/N A41 OmpAsignal : topovector