Wisconsin-Madison/20 May 2009

May 20, 2009
Ex 2:	Growth on following plates

1.	pBAD33 –Cm resistance

2.	pTrc 99A – Amp resistance

3.	pBt2BAD18 – Kan resistance

Made liquid cultures of the three plasmids

Other:

Made antibiotics: Amp, Cm, Kan

Research

Ex 1: 	MiniPrep of NudF and YhfR acroding to miniprep protocol Results: out of RNAase and got bad results from our gel



- mini preparation of pNudF and pYhfR according to the mini prep protocol of Scott Walhovd, bad results b/c no RNAase - NP - inoculated liquid cultures of Dan's plasmids from yesterday in LB media with appropriate antibiotics - NP - made freezer stocks of pNudF and pYhfR - NP