Team:Virginia/15 June 2009

Overnight Results

 * Plate of A09-2 dilute (PRE BFP) had colonies
 * Still not seeing visible BFP

Today

 * Ordered Arsenic binding peptide (ABP) as pair of oligos
 * BB prefix & suffix
 * Two repeats of CCGCG (Cys-Cys-Gly-Cys-Gly) with Lys-Gly-His linkers after each
 * Complete AA sequence: CCGCGKGHCCGCGKGH
 * Complete DNA sequence: gaattcgcggccgcttctagATGTGTTGTGGTTGCGGTAAAGGTCATTGTTGTGGTTGTGGTAAAGGTCATtactagtagcggccgctgcag
 * Getting quote for PCS in DNA 2.0's pJ express in-house expression vector.
 * with BB suffix, no BB prefix
 * Can add the prefix later with PCR, but its stop codon would interfere with expression