Team:UNICAMP-Brazil/Notebooks/September 23

F plasmid recircularization

 * The ligation reaction was assembled according to Protocol 11. Both the plasmid samples were used in recircularization. The reaction tubes was kept O/N at 4°C.

Gabriel

finO and finP - Still Trying to Confirm our Biobricks

 * Today we performed minipreps from yesterday's inoculated cultures, according to Protocol 2.

Marcelo

CeaB and CeiB: colony PCR

 * We got apparently good transformations but we have to prove the correct position of DNA inserted. So, we performed colony PCR with right primers.




 * We didn’t get the band size expected and we found unspecific amplification, therefore we will make miniprep and we will try PCR again.

Ane