Template:Team:KULeuven/11 August 2009/VanillinReceptor


 * We also received another strain of Agrobacterium. We brought this new strain in culture.
 * To make things clear the genes from the first Agrobacterium will from now on be called A,B,G and R for respectively virA, promotor region virB, virG and rpoA. For the second Agrobacterium we will use W,X,Y and Z to indicate the same genes.
 * We achieved good results for genes B,G and R. There were clear white colonies after plating the competent cells with the TOPO vector on X-gal agar plates with ampicillin. We isolated the good colonies again.
 * There was no result for A. This was probably due to a fault with the competent cells. So we started TOPO transformation by heatshock all over for A.