Template:Team:KULeuven/24 August 2009/BlueLightReceptor

4. Enting of liquid cultures with kanamycin and
 * 1) Plates with LigA were put under blue light. The LEDs were put on their max capacity.
 * 2) Restriction digest with
 * 3) *tubes (1,3,5,7,9) of LigA (BLP + ) cut with EcoRI en PstI
 * 4) *promotor cut with EcoRI en SpeI (4x)
 * 5) Gel electrophoresis with the RD of LigA and followed by a gel extraction:
 * 6) * Note: tube 5 of LigA was loaded poorly on the gel and could not be used.
 * 7) * Note: the samples of promotor were barely visible. Only 2 of the 4 samples were recovered by extraction.