Team:Wisconsin-Madison/Polymerase Chain Reaction (PCR)

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Polymerase Chain Reaction (PCR)


 * To amplify and to add BioBrick Restriction sites to your DNA your run a PCR of your gene.
 * You must first design Primers for you gene.

Tips:

EcoR1 (E)
 * 5’-	G A A T T G	-3’
 * 3’-	C T T A A G	-5’

Xba1 (X)
 * 5’-	T C T A G A	-3’
 * 3’-	A G A T C T	-5’

Spec1 (S)
 * 5’-	A C T A G T	-3’
 * 3’-	T G A T C A	-5’

Pst1 (P)
 * 5’-	C T G C A G	-3’
 * 3’-	G A C G T C	-5’

X and S have same sticky ends

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