Virginia Commonwealth/14 July 2009

Results
Maria and Afton
 * All of the promoters showed up on the gel electrophoresis in a mostly straight line.
 * Transformation Results

Trentay 13:44, 14 July 2009 (UTC)
 * Miniprep Results:


 * Gel Electrophoresis Results

Kevin and Adam Bussingkm 15:21, 14 July 2009 (UTC)
 * Miniprep results:

Craig and Clay
 * The ampicillin plates left overnight again displayed no growth. The electrotransformed parts will be re-plated onto ampicillin-selective plates as well as non-selective plates.

Tasks
Maria and Afton Trentay 15:13, 14 July 2009 (UTC)
 * Digest promoters: 101, 102, 103, 104
 * Possible Ligation of promoters with parts J06702, pSB4C5

Kevin and Adam Bussingkm 15:20, 14 July 2009 (UTC)
 * perform complete digestion and ligation
 * construct biobrick device (pSB4C5, BBa_E0240, BBa_J23100), and (pSB4C5, BBa_E0240, BBa_J23106)

Craig and Clay
 * The electrotransformed parts will be re-plated on ampicillin plates as well as on non-selective plates. Growth on non-selective plates will prove the existence of viable cells.

Wetlab
Maria and Afton Trentay 21:01, 14 July 2009 (UTC)
 * Spectophotometry of promoters: 101, 102, 103, 104, 105, 107
 * Digestion/Ligation of promoters
 * Electrophoresis of promoter digestions: 101, 102, 103, 104, 105, 107, (100)
 * Electrotransformation of ligated parts
 * two Cm plate had an unidentified growth in the refrigerator.
 * however, negative controls from the same plate stock show antibiotic is working
 * Picked colonies for overnight culture of J23106 w/ J06702 reporter
 * Made 3 overnight 5mL cultures of NEB 10 beta cell stock to prepare electrocompetent cells

Kevin and Adam Bussingkm 15:22, 14 July 2009 (UTC)
 * pSB4C5, BBa_E0240, and BBa_J06702 were frozen in box C-11 of the glycerol stocks

Craig and Clay
 * The electrotransformed parts were again plated on ampicillin-selective plates. They were also plated on non-selective media.  One hundred microliters was used for each plate rather than the standard 35 microliters.