Team:Warsaw/Calendar-Main/4 September 2009

Acquiring the internaline A gene from Listeria monocytogenes st. EDG-e genome
Jarek

Tasks:
 * Electrophoretical segregation of PCR products from previous day in 0,8% agarosis gel


 * Preparation of another PCR reaction with L.monocytogenes genome DNA

Results:
 * There were no visible products on gel, it might happened that I accidently forget to add one of the substrates to reaction.

Assembly of endosomal detection operon Marcin

Task 1: Methods: Bistable switch 1 testing Ania Tasks:    
 * Transformation of chemocompetent E. coli TOP10 strain. Construct to transform:
 * BBa_K177037 on pSB1A3 plasmid
 * cro CDS on pSB1A3
 * Bax CDS on pSB1A3
 * Detailed protocol of transformation is described here