Virginia Commonwealth/26 June 2009

Results

 * Plate growing pSB3K3 plasmid grew well. There was no fluorescence expressed.
 * Plate growing the device plasmid pSB3K3, BBa_J23102, and E0240 grew well. There was no fluorescence expressed.

Tasks

 * Pick colonies and grow overnight.
 * Confirm the growing colonies using GFP and RFP reporter.
 * Order DNA and IPTG
 * Additional promoters were designed. These possessed an up element that is proven to bind with the alpha subunit of RNAP by multiple sources.

Wetlab

 * IPTG stock was prepared. Since only 0.746g of IPTG stock was available, only 2.64 mL was prepared. -
 * IPTG stock (5uL) was added to 5 mL of bacteria that was grown overnight with pSB3k3 backbone (to induce the expression of RFP). After several hours no fluorescence was expressed.  3 mL LB+KAN was added to each culture so that the cells could re-enter log phase growth.  3 uL IPTG was also added to each culture to maintain concentration.