Virginia Commonwealth/10 August 2009

Results
Maria and Afton Trentay 11:31, 10 August 2009 (UTC)
 * Transformation was successful. Results:

Tasks

 * 1 Today parts were digesting and ligating into full plasmids then transformed into E. coli NEB10&beta;. UP element D1 was ligated onto constitutive promoter BBa_J23101 on plasmid pSB1C3.  UP element with promoters D2 - D9 were ligated to RET BBa_J06702 on pSB1C3.

Bussingkm 13:45, 10 August 2009 (UTC)
 * 2

Wetlab

 * 1 Wetlab work went relatively smoothly. E. coli transformations occurred at roughly 6:00 PM.  During digestion, I did not start the hot water bath soon enough but gel electrophoresis showed the correct cutting before ligation.  Procedure was followed well. Bussingkm 20:09, 11 August 2009 (UTC)
 * 2