User:DavidC/19 August 2009

Wednesday the 19th
Mycobacterium avium transplanting on Lowensteim medium (two tubes).

First polymerase chain reaction (PCR) on the lambda phage D protein.
Cycle:

Hot-Start : 94°C, 2 minutes. DNA denaturation : 94°C, 1 minute. Primers annealing : 59°C, 1 minute. Primers extension : 72°C, 1 minute.

Electrophoresis
85 Volt, 15 minutes. 105 Volt, 40 minutes. Ladder fermentas 1 Kb. Sample : D protein

No results.