Lab June 29 2009

Mon June 29, 2009

filled distilled water tank

got materials from central services and our glassware, etc. collected

microfuge came from 301 lab

balance came from micro 200 lab

tryptone, nacl, yeast, and agar all from central services

Antibiotics (only amp and kan to start) are from Nano's lab

Prep 4 x 500 ml LB broth to send to central services for autoclaving. (5 g NaCl, 5 g tryptone, 2.5 g yeast extract, 3.75 g agar)

autoclave 250F, 22psi, 30 minutes

prepare 2 x 500 ml worth of petri dishes with ampicilin (from 50 mg/ml stock, to a final concentration of 250 ug/ml, so 2.5 ml per 500ml beaker)

prepare 500ml worth of petri dishes with kanamycin (from 12 mg/ml stock, to a final concentration of 30 ug/ml, so 1.25 ml per 500 ml beaker)

prepare 500 ml worth of petri dishes without antibiotic

collected dry reagents for making the Qiagen buffers, but did not begin mixing solutions.

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