Week8



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1. Repeated FACS run of CFP and YFP with 100, 250, 500 and 1000 microM/ml IPTG

2. Preparation of requirements for transformation. (0.1M cacl2 soln., Ice cold centrifuge tubes.) Kept autoclaved

eppendorf tubes and tips into freeze.

3. Kanamycin stock 100X (X = 50 microg/ml)