Wisconsin-Madison/2 July 2009

July 2, 2009
Other: Brian Meeting

Research

Proposal for Genes Abroad

Ting took her MCAT!

Ex 15: Inducing Various Modified (triple) E.Coli Strains

2. MG1655 W: Trip – GROWTH ON PLATE – SUPPOSED TO DIE FROM TOXICITY OF INTERMEDIATES!

SO MUCH HERE IS SO SO WRONG – ran out of Triple plates

1. Control 33+99: Cm+Amp  - EH

2. MG1655 W: Trip  - Maybe some type of Isoprene

3. MG1655 Delta Ara: Trip – Contamination of Sample  - could not analyze 4. BL21 (DE3): Trip - Maybe some type of Isoprene

NO ISPOPENTENOL - See meeting for game plan

Primers: Primer Design for MevT and MetK

Do not need primers for Photosystem Promoter – having it sequenced

Ex 18: pTaq Induction and experiments

MiniPrep of pTaq

Ex 10: Cyanobacteria

Tested freezer stocks of 7942 (25% glyc and 50:1 centrifugation/resuspention) again on BG-11 plates with appropriate plating (parafilm wrap) - NP