Team:Gaston Day School/12 June 2009

 Spun tubes #1-8 for 3 minutes Combined tubes #1-4 and tubes #5-8 Added 300x TENS Mix by inversion Added 150x sodium acetate Mix by inversion Spun for 2.5 minutes Transfered Sup to clean tube</dt> Added 1 mL of EtOH (ethanol)</dt> Spin for 5 minutes</dt> Washed pellet in 70% EtOH 300x</dt> Spin for 5 minutes</dt> Resuspend 30x TE</dt> Digest w/ EcoRI</dt> 5x DNA</dt> 2x Buffer</dt> 11x dH2O</dt> 1x EcoRI</dt> Incubate at 37C at 11:45AM</dt> Saw no bands</dt>