August/12 October 2009

1. min prep sample       conc. C2              14 ng/uL C3              14 C4              13 68             182  68              118  69              161  69              119  74              250  74              243  78              105

2.digation

K204072 K204075

K204085

K204086 K204087 K204088 K204089 K204090

↓ 37degree, 3hr gel cut & purification ligation O/N

3. min prep sample     conc, 58             127 ng/uL 58             229  62              79  76              202  77              175   77              184

4.transformation sample 79, 80 , 81 , 82 , 83 , 84 , 68 , 71 , 67 , 73

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