Team:Brown/Project All Together




 * 1) Mast cells release histamine during the allergic response.
 * 2) Histamine binds to our re-engineered histamine receptor.
 * 3) This receptor’s intracellular kinase domain EnvZ phosphorylates transcription factor OmpR.
 * 4) OmpR turns on transcription of DNA under the OmpC promoter.
 * 5) The genes for rEV131 with its attached secretion signal are transcribed.
 * 6) After translation, the signal peptide causes rEV131 to be secreted into the extracellular fluid.
 * 7) rEV131 sequesters histamine, preventing it from interacting with the human histamine receptors.
 * 8) The transcription and secretion of rEV131 continues as long as histamine is present in the extracellular fluid. When histamine concentration returns to its pre-allergic response state, production of rEV131 stops because the initiating ligand histamine is no longer present.

 