Team:Warsaw/Calendar-Main/26 July 2009

Assembly of endosomal detection operon Marcin Task 1:  Transformation of chemocompetent E. coli strain DH5&alpha;  Methods:  Detailed protocol of transformation is described here. The only modification is usage of total volume of ligation mixture prepared 20.07.09 petri dish were hold in 37&deg;C for 16 hours 

Construction of K177012  operon1_part2

Ania Tasks:  Set up liquid cultures from previously transformed cells to plasmid DNA extraction. </li> Transform cells with repeated RBs.3LacI ligation.</li></ul>