Team:CityColSanFrancisco/Material

Bacteria
Chemotropes which reduce Fe3:
 * S. Oneidensis 
 * R. ferireducines 

Phototropes which Oxidize Fe2+ 


 * R. palustris TIE-1 strain – hopefully transformed!

Genetic manipulation 
 * P. aeruginosa- source of OprB gene (membrane porin sequence).
 * pJQ200SK plasmid suicide vector for R. palustris. 
 * E. coli- for plasmid production/ purification. 

Primers for isolating and manipulating genes
Pseudomonas aeruginosa OprB primer Forward: 5' ATGTACAAGAACAAGAAAACC 3' Reverse: 5' TCAGAACACCGTCTGGATC 3'

Mutegen primer to change GCTGCA to GCAGCA in OprB gene so it can be a biobrick Forward: 5' CTGTACCAGCCGCaGCAGGACACCGAG 3' Reverse: 5' CTCGGTGTCCTGCtGCGGCTGGTACAG 3'

R. palustris OprB primer -R. palustris might be upregulated Forward: 5' ATGCTGCCGTCGCGCTGGCT 3' Reverse: 5' TCAGAACTGCGTGCGCAGTCG 3'