Team:UNICAMP-Brazil/Notebooks/October 18

PY Promoter - Colony-PCR

 * We got one single colony from the transformation we did yesterday!


 * We performed a colony-pcr to confirm that this colony has our plasmid.


 * We used 2 pairs of primers - VF/VR and PY primers - and analyzed the results of our PCR in an agarose gel:




 * The expected size for PY1 + RFP + BBa_B0015 amplified with VF/VR primers is 1279 bp.


 * The expected size for PY1 + RFP + BBa_B0015 amplified with PY primers is 133 bp.


 * As we can observe in the gel photo, the colony presented bands compatible with the size expected for both pairs of primers!!!! =)

Fabi and Léo

Yeast experiments

 * We did miniprep of 10 inocula of pDLD+YFP and 10 inocula of ADH1+YFP. We transformed the plasmids in competent yeast. Hope to find colonies tomorrow! =)

Raíssa and Taís