Team:PKU Beijing/Project/AND Gate 2

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Point Mutation of PhiR73 and Rescue it by supD tRNA
PhiR73 is sucessfully mutated on its first serine residue, and in another clone, we mutated it on both its first and second serine residues. SupD tRNA is coexpressed inside of the cell. It is obseved that the amber mutation can be successfully rescued by the SupD tRNA, so that GFP is expressed. FOR MORE, PLEASE LOOK AT AND Gate 2 Design / AND Gate 2 Result Pages.