User:DavidC/27 August 2009

DNA extraction
Samples : C0012 = 195 ng/µL; R0040 = 130ng/µL; B0030 = 270ng/µL; P1002 (1)= 80ng/µL; P1002(2)= 200ng/µL.

Brick : BBa_R0040
DNA (1µg) = 7,69µL Buffer Takara H 10x = 2µL H20 = 8,31µL Spé 1 (Takara) = 1µL Eco R1 (Takara) = 1µL 1h at 37°C.

Brick : BBa_B0030
DNA (1µg) = 3,64 µL Buffer Takara H 10x = 2µL H20 = 12,36µL Xba 1 (Takara) = 1µL Eco R1 (Takara) = 1µL 1h at 37°C.

DNA electrophoresis
85 Volt, 15 minutes. 105 Volt, 40 minutes. Ladder fermentas 1 Kb.

No results, we do not have any plasmid restriction digest.