Virginia Commonwealth/12 August 2009

Results
I will wait for fluorescence to pick colonies.

Maria and Afton Trentay 17:55, 12 August 2009 (UTC)
 * Results of transformation were not good
 * A gel will be run today on the digests to see how they look.
 * Plates will be kept for a few more days in case the bacteria are growing slowly

Tasks
Maria and Afton Trentay 17:55, 12 August 2009 (UTC)
 * Miniprep more pSB1C3 p1010
 * Take Spectrophotometry measurements for J06702, pSB1C3, 101, 105, 106, 107
 * Digest promoters 101, 105, 106, 107 along with pSB1C3, J06702
 * Run a gel on the digests
 * Everything fit on the gel except for 106 and 107

Wetlab
Kevin and Adam
 * I don't think that the no growth constructs were not successful. The last ligation reaction cleaned out the PCR product for D1-9.  Will perform another PCR reaction today so the ligation can be done again.
 * Purified PCR product did not show up on gel. I will redo reaction tomorrow. Bussingkm 22:26, 12 August 2009 (UTC)

Maria and Afton Trentay 19:44, 12 August 2009 (UTC)
 * Miniprep was done
 * Spectrophotometry was done
 * Digest was done
 * Gel was run