ULB TEAM/1 June 2009

Report iGEM of July 1, 2009 We began the first morning by a recall presentation from the principles of molecular biology by Gilles our advisor. He also pointed out us the various tools of molecular biology which we will have probably to use during handling: •	PCR, •	synthesis/sequencing of DNA, •	microscopy, etc It will be useful for us also to reflect, in the case of our subject, for the excretion of our product, with one or the other sequence signal, with resistances to antibiotics, a possible system poison-antidote,… All that depending obviously on the way in which will arrange the project. Our frame is : Escherichia coli. With regard to the prices decreed for the contest, to obtain a certain medal, it is necessary to carry out a certain number of tasks; in short (for more details: http://2009.igem.org/Judging/Judging_Criteria): For a bronze medal, it is necessary: • To fill a report/ratio of project •	To create a wiki •	Te create a poster and to present the project orally •	To imagine a new part Biobrick (theoretical point of view) with informing well/describing! (note: PoPS = polymerase a second, the measuring unit) For a money medal, it is necessary: •	To prouve that it walk •	 To characterize a new part (manipulation point of view of) For a gold medal, it is necessary: •	To characterize or improve an already existing part •	To help another team •	To develop an ethical aspect (for our project, one could consider the energy aspect of the manufacture of the adhesive) In addition to these medals, there are different other prices detailed here. One also speaks about “under-competitions” among which it is necessary to choose in which field between our subject. A priori, our project is part of in the category “Best manufacturing project”. For the absents : this page gives very full with super information interesting, it would be well to take time to read the contents of it to have the clearer ideas for certain things and according to your basic training. At the end of the morning and until the following day afternoon, we carried out some basic handling of molecular biology which will be more useful to us in the continuation of the project: •	Insertion, by electroporation, of plasmides for the fluorescent protein expression (RFP, GFP.) in E. coli (= transformation) + visualization under the microscope. PCR + freezing of agarose •	Extraction of DNA + restriction with one or two enzymes + migration on freezing of agarose. In end-of-day of Thursday, meeting to decide tasks to make soon, essentially biblio, to see list written by Hugo. Each one is held to get information about a little all that was defined, by making a SORTING of information before subjecting it to all so that one is not drowned under the mass of information. A meeting for pooling and continuation of the project is envisaged Wednesday July 8 with 8:00 with the library of Solbosh. Lastly, a calendar was also drawn up in order to visualize which leaves when on holiday and/or works and/or has one second session.