Virginia Commonwealth/14 August 2009

Results

 * Gel did not show any results.

Maria and Afton
 * Overnight cultures of J23100 and J23101
 * J23100 was vaguely pink
 * J23101 was slightly pink
 * They should probably be left for another day to grow to see more expression

Spectrophotometry Measurements for Digestion
 * Because the concentrations were so low for J23113, J23116, and R0011, more frozen stocks will be grown up and Miniprepped. R0040, J23110 have more minipreps in stock in the freezer
 * Electrophoresis Gel Results
 * It is really difficult to see promoters from digestions when run on the gel

Trentay 22:05, 17 August 2009 (UTC)

Tasks

 * Today I am running a gel on the purified PCR products to confirm the reaction. The gel is 1% agarose, running at 100 volts for 45 minutes.

Maria and Afton Trentay 23:05, 17 August 2009 (UTC)
 * Make Cryogenic stock of the overnight cultures J23100, J23101
 * Miniprep J23100 and J23101
 * Take Spectrophotometry of J23110, J23113, J23116, R0011, R0040
 * Digest J23110, J23113, J23116, R0011, R0040
 * Run a gel on Digests: J23106, J23107, J23110, J23113, J23116, R0011, R0040, Miniprepped 110
 * Miniprepped J23110 was chosen because it had the greatest amount of DNA at the time
 * Ligate J23110, J23113, J23116, R0011, R0040 with pSB1C3 w/p1010, J06702 (RET)
 * pSB1C3 w/p1010, J06702 (RET) were digested and were run on a gel August 12 and was reused for the ligations done today
 * Ligations will be frozen until cuvettes come in

Wetlab
Maria and Afton Trentay 23:08, 17 August 2009 (UTC)
 * Miniprep was done
 * LB was made
 * Spectrophotometry was done
 * Digestion was done
 * Next time BSA and other BioBrick Assmbly Materials are aliquoted, they should be placed in smaller tubes, possibly PCR tubes, because it is very difficult to see the samples
 * Electrophoresis Test was done
 * Smaller wells were used so more samples could be placed on one gel (15 wells instead of 8)