Team:Warsaw/Calendar-Main/20 September 2009

Assembly of endosomal detection operon Marcin

Task 1: Prepare bacterial culture to isolate BBa_K177044 from pSB1A3.

Task 2: Another ligation of BBa_K177044

Methods: 10 μl digested insert 8 μl digested vector 5 μl Tango buffer(Fermentas) 2 μl ATP 10 mM solution 4 μl MQ water 1 μl ligase T4 (Fermentas)
 * Ligation mixtures composition:
 * Ligation was carried out about 20 hours and subsequently inactivated