Team:Warsaw/Calendar-Main/18 September 2009

Assembly of endosome detection module: Marcin Task 1: Transformation of TOP10 bacterial strain with BBa_K177044 on pSB1A3 plasmid Methods:
 * Ligation mixture was thermally inactivated (done by Kuba)
 * Detailed protocol of transformation is described here

Assembly of fusion proteins Marcin Task 1: Isolation of pSB1A3 plasmid containing BBa_K177028 Methods: Task 2: Restriction digest of previously isolated plasmids:
 * Plasmids were isolated using the A&A plasmid mini kit. Detailed procedure of the isolation is described here

Methods: 3 μl purified plasmid DNA product 1 μl EcoRI (Fermentas) 1 μl PstI (Fermentas) 2 μl Buffer Tango (Fermentas) 13 μl MQ water
 * Reaction mixture composition:
 * Reactions were carried out 1.5 hour

Results: Really disappointing, none of the attemps to ligate the signal peptide were successful.