Team:PKU Beijing/Notebook/AND Gate 1/Core/Rencheng Gao

Notebook > AND Gate 1 > Core > Rencheng Gao's Note

2009.8.17
0:50 Bacteria Culture: 11N-pcrS6(1~4), 2K-pcrS6(1~4), pla2+SupD(1~4), pcr2+SupD(1~4)

12:00 Miniprep: 2GpcrS6(1~4), 5JpcrS6(1~4), 1HpcrS6(1~4)

17:00 Double-enzyme digestion assessment: 2GpcrS6(1~4), 5JpcrS6(1~4), 1HpcrS6(1~4)

24:00 Bacteria Culture: 1H-pcrS6(5~7), 11N-pcrS6(5~7), 2K-pcrS6(5~7), pla2+SupD(5~7), pcr2+SupD (5~7)

2009.8.18
11:00 Miniprep: plaSupD(5~7), pcrSupD(5~7)

14:35 Double-enzyme digestion assessment: plaSupD(5~7), pcrSupD(5~7)

14:50 Ligation: Insert: 11N, 2K, 1H Vector: pcrS6

21:00 Transformation

2009.8.19
15:00 Double-enzyme digestion assessment Plasmid: plaS6, pcrS6, 5JpcrS6, 2GpcrS6

22:00 Bacteria culture: 2K(1~5), 1H(1~5)

22:30 Gel Purification: plaS6, pcrS6, 5JpcrS6, 2GpcrS6

00:30 Ligation Insert: plaS6, pcrS6, 5JpcrS6, 2GpcrS6 Vector: 1-7G

2009.8.20
12:00 Send samples for sequencing: plaS6, pcrS6, 5JpcrS6, 2GpcrS6

14:00 Miniprep: 2KpcrS6(1~5), 1HpcrS6(1~5), 11NpcrS6(1~5)

17:00 Double-enzyme digestion: 2KpcrS6(1~5), 1HpcrS6(1~5), 11NpcrS6(1~5)

22:00 Store the strain: 2KpcrS6(1), 1HpcrS6(1), 11NpcrS6(1)

2009.8.21
9:30 Send samples for sequencing: 2KpcrS6(1), 1HpcrS6(1), 11NpcrS6(1)

2009.8.22
11:00 Bacteria culture: pcrS6(1~4), plaS6(1~4), 2GpcrS6(1~4), 5JpcrS6(1~4)

15:30 Double-enzyme digestion: 2KpcrS6(1), 1HpcrS6(1), 11NpcrS6(1)

22:00 Gel purification

23:00 Miniprep: pcrS6(1~4), plaS6(1~4), 2GpcrS6(1~4), 5JpcrS6(1~4)

1:00 Ligation: Insert: 2KpcrS6(1), 1HpcrS6(1), 11NpcrS6(1) Vector:1-7G

2009.8.23
13:00 Double-enzyme digestion assessment: pcrS6(1~4), plaS6(1~4), 2GpcrS6(1~4), 5JpcrS6(1~4)

15:00 Transformation

0:00 Bacteria culture: 5JpcrS6-low-copy, 2GpcrS6-low-copy

2009.8.24
11:00 Bacteria culture: 2KpcrS-low-copy(1~4), 1HpcrS-low-copy(1~4), 11NpcrS-low-copy(1~4)

14:00 Double-enzyme digestion (back insert): T7pro-GFP Double-enzyme digestion (back vector): 5JpcrS6-low-copy, 2GpcrS6-low-copy

21:00 Gel purification

22:00 Miniprep: 2KpcrS-low-copy(1~4), 1HpcrS-low-copy(1~4), 11NpcrS-low-copy(1~4)

23:30 Ligation Insert: T7pro-GFP Vector: 5JpcrS6-low-copy, 2GpcrS6-low-copy

24:00 Double-enzyme digestion: 2KpcrS-low-copy(1~4), 1HpcrS-low-copy(1~4), 11NpcrS-low-copy (1~4)

2009.8.25
13:30 Transformation: 2G-T7pro-GFP, 5J-T7pro-GFP

17:00 Strain storage: 2KpcrS-low-copy, 1HpcrS-low-copy, 11NpcrS-low-copy

2009.8.27
14:30 Inducement: AND gate(5J, 2G) HSL: 10-4M Arabinose: 10-4M

17:00 Double-enzyme digestion (front insert): 2KpcrS6(1), 1HpcrS6(1), 11NpcrS6(1)

2009.8.28
13:00 Inducement: AND gate(5J, 2G) HSL: 10-4M Arabinose: 10-4M

13:40 Double-enzyme digestion (front insert): T7pro-GFP

2009.8.29
13:00 Bacteria culture: 1HpcrS(1~3), 11NpcrS(1~3), 2KpcrS(1~3), 5JpcrS(1~3), 2GpcrS(1~3)

21:45 Ligation Insert: 5NT7ptag, 2MT7ptag, 2IT7ptag, 1JT7ptag Vector: pcrS6

2009.8.30
13:00 Miniprep: 1HpcrS(1~3), 11NpcrS(1~3), 2KpcrS(1~3), 5JpcrS(1~3), 2GpcrS(1~3)

14:00 Double-enzyme digestion assessment: 1HpcrS(1~3), 11NpcrS(1~3), 2KpcrS(1~3), 5JpcrS(1~3), 2GpcrS(1~3)

17:00 Bacteria culture: 5NT7ptag(1~3), 2MT7ptag(1~3), 2IT7ptag(1~3), 1JT7ptag(1~3)

21:30 Strain Storage: 2K(3), 5J(2), 1H(1), 11N(1), 2G(2)

22:00 Double-enzyme digestion (front vector): 2K(3), 5J(2), 1H(1), 11N(1), 2G(2)

2009.8.31
13:00 Miniprep: 5NT7ptag(1~3), 2MT7ptag(1~3), 2IT7ptag(1~3), 1JT7ptag(1~3)

14:00 Gel purification: 2K(3), 5J(2), 1H(1), 11N(1), 2G(2)

16:00 Send samples for sequencing: 2K(3), 5J(2), 1H(1), 11N(1), 2G(2)

17:00 Ligation: Vector: 2K(3), 5J(2), 1H(1), 11N(1), 2G(2)

17:30 Double-enzyme digestion: 5NT7ptag(1~3), 2MT7ptag(1~3), 2IT7ptag(1~3), 1JT7ptag(1~3)

1:30 Strain Storage: 2I(1), 2M(2), 1J(2), 5N(1)

2009.9.1
13:30 Double-enzyme digestion (Front Vector) Plasmid: 5N, 2M, 2I, 1J

21:30 Culture the bacteria: 5J, 2K, 1H, 2G-T7pro GFP

23:20 Ligation Vector: 5N, 2M, 2I 2J Insert: T7pro-GFP

2009.9.2
14:00 Inducement: 5J, 2K, 1H, 2G, 11N

22:00 Double-enzyme digestion Plasmid: 1JpcrS, 11NpcrS, 2IpcrS, 5JpcrS, 2MpcrS, 1HpcrS, 5NpcrS, 2GpcrS, 2KpcrS

2009.9.4
15:30 Send samples for sequencing: 5N, 2M, 2I, 1J

15:45 Gel purification: 11N, 1H, 2M, 1J, 2G, 5N, 1J

16:00 Ligation: Vector: 1-7G Insert: 11N, 1H, 2M, 1J, 2G, 5N, 1J

2009.9.5
17:00 Double-enzyme digestion Plasmid: 11N(1), 2M(2), 2K(2), 1H(1), 5J(1), 1J(2), 2G(2)

2009.9.6
16:00 Double-enzyme digestion (5J, 2I, 1J, 2K, 1H, 5N, 2M, 11N, 2G)

22:00 Culture the bacteria: 5N(3), 2I(1), 2I(3)

00:00 Ligation: Insert: 5J, 2K, 1H, 2M, 2G Vector: 1-7G

2009.9.7
14:00 Mini-prep: 2I(1), 5N(3), 2I(3)

16:30 Double-enzyme digestion: 1J

17:00 Prepare competent cells

21:00 Transformation: 2G, 2I(1), 2I(3), 5N(3)

2009.9.8
14:00 Culture the bacteria:

15:00 Ligation Insert: 2M, 1H Vector: 1-7G

2009.9.9
01:00 Double-enzyme digestion Plasmid: 11N, 5J, 2K

20:30 Inducement: 2G, 5N, 2I

24:00 Mini-prep: 1J(1-3), 2M(1-3), 1H(1-3)