Team:Freiburg bioware/Notebook/April

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   Home   The Team  <ul> Overview</a></li> Portraits</a></li> </ul> </li>  <span class="r"> The Project </a></li> <span class="l"> <span class="t">Human Practice </a> <ul> D'oh! Something's missing here.</a> <ul> 1</a> </li> 2</a> </li> 3</a> </li> </ul> </li> <li><a href="#">4</a></li> <li><a href="#">5</a></li> </ul> </li> <li><a href="http://2009.igem.org/Team:Freiburg_bioware/Notebook" class="active"> <span class="r"> Notebook </a></li> <li><a href="http://2009.igem.org/Team:Freiburg_bioware/Collaboration"><span class="l"> <span class="t">Collaboration </a></li> </ul> <h2 style="border-bottom: none;" class="art-PostHeaderIcon-wrapper"> <img style="width: 28px; height: 25px;" alt="" src="http://2009.igem.org/wiki/images/2/2a/Freiburg09_Post_tanne_2.png" /> April 24th April 12:30 - 18:00 o'clock Manuel Literaturrecherche zu FokI.

Entstandene Ideen: <ul> <li>three oligo's: two for binding the monomers and one to create a dsDNA where FokI can bind.</li> <li>two oligo's: one for binding one monomer, the second for binding the second monomer and to create dsDNA (one monomer has to be inactivated).</li> <li>one oligo: both binding sites of the monomers on one oligo (also one monomer has to be inactivated).</li> </ul>

The 196 C-terminale aminoacids of FokI were used from other groups (see reference 1). Primersequences are also provided in the publication.

Reference 1: Freiburg09_FokI_Zn-Finger_1996.pdf

</a>29th april 21o'clock R&uuml;diger

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