September/8 September 2009

1.colony check ( sample No.) (no. of colonies) 1-1D               100< 10-D               200~ -E               200~ 9               0         24                0         27                1         28                1         29                0         30                0

2.Min prep Result of Nanodrop concentration check (sample No.) (concentration) 18-A          8.5 -B          16.5 23-A          19.0 -B          28.6 22           17.4      22            15.6      25-A          13.3 -B         20.2

3.digestion and ligation, and ligation check

K204033

K204034

K204035

K204036

↓ 37degree, 3hr ↓ Agarose Gel Electro-phoresis UP  : No.5, 18A , 18B , 22 , 23A , 23B , 25A , 25B , 26 , ladder DOWN : No.33, 34 , ladder

↓ 16 degree O/N

4.digestion and ligation

K204037

K204038

K204039

K204040

↓ 37degree, 3hr ↓ Agarose Gel Electro-phoresis UP  : No.35, 36 , 37 , 38 , ladder DOWN : No.39, 40 , 40 , ladder

↓ 16 degree O/N

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