Lab July 28 2009

0:00 am - Derek

Checked on the plates from yesterday. No growth on the untransformed DH5alpha on LB+amp. Should give it an extra day since the contamination only showed up after a weekend of incubation. This is of only academic interest, though, since our old batch of competent cells seem fine.

10 ng/ul - TNTC, small, packed colonies

1 ng/ul - TNTC, large more spaced colonies

.1 ng/ul - countable, large well-spaced colonies

I'm afraid I didn't have time to actually count the .1 ng colonies, but we should do that to get a transformation rate. (leave them in the fridge for me if nobody else gets time to count them...)

12:50 pm - Kyliah

The 0.1 ng/μL plate had roughly 225+/-10 colonies of decent size on it, and a host of pinprick colonies - maybe the untransformed cells had enough time to start growing?

Rehydration Protocol for test part BBa_I13521 in 15μL dH2O; nanospec'd as 62.5ng/μL (2μL sample had A260 = 1.251, A280 = 1.429) but there was a dye in the solution - we now know dye is in all registry parts.

Made a 1/100 dilution of the plasmid sample and transformed 2x100μL transformed cells with 2μL according to the Transformation Protocol.

Plated six plates - 200μL, 100μL, and 50μL from each aliquot - help us calibrate exactly how much we need

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