Wisconsin-Madison/10 July 2009

July 10, 2009
Ex 20: New Cloning Methods

Inoculate DH1 (Blank – No resistance)

Redone Digestion from Yesterday:

Left to right: NudF 1b undigested-digested- NuF2B undigested-digested- Ladder- choline 1 undigested - digested- backbone undigested- digested

1. NudF – (560+2157=) 2717 bp

2. Choline – (2000+2157=) 4157 bp

3. Backbone P1 – 2157+1069 bp

4. (pVP68K): gsSDMT (Pst1) – (931+5212=) 6143 pb



Ex 10: Cyanobacteria 

1. Spray everything with 70%

2. Add 9 mL Media A to cultures (7/9/09)

3. Add 7.35 uL CM to cultures (7/9/09)

4. Shake - 30C Cyano Room

Ex 19: Parts from Registry

Inoculate 1.O22

Other: 

Research 1st Antiporter gene has cut with Pst1

2nd Antipoter gene has no iGEM cut sites

Primer Design:

1st Antiporter finalized

2nd Antiporter finalized

New ProU finalized Primers Ordered: MevT, MetK, ProU again, Gssdmt, Chris's two antiporter gene

Ex 18: pTaq

Made two protein gels

Ran Taq Protein

Stained protein gel

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ProU, ProV cutting and cloning