Virginia Commonwealth/13 July 2009

Results
Maria and Afton
 * Digestion was successful on parts J23106 and J06702
 * Gel Electrophoresis Results

Trentay 18:30, 13 July 2009 (UTC)

Craig and Clay
 * The parts that were plated on Friday were unsuccessful. Electrotransformation will be performed again.

Tasks
Maria and Afton Trentay 18:34, 13 July 2009 (UTC)
 * Make daily planner for experimentation
 * Perform Electrophoresis test on Digested parts J23106 and J06702
 * Ligate parts: J23106 (promoter), J06702 (RET), pSB4C5 (backbone)
 * Transform ligated DNA into e. coli NEB 10 beta

Kevin and Adam
 * Back bone pSB3K3 is a bad part. Cultivate backbone pSB4C5 from glycerol stock.

Craig and Clay
 * A second attempt at electrotransformation of parts BBa_I742111, BBa_K118024, and BBa_K118025 will be performed today. These electrotransformed parts will be plated on ampicillin plates.
 * If the above is unsuccessful, the parts will be re-plated on the ampicillin plates and on non-selective plates.

Wetlab
Maria and Afton
 * Gel Electrophoresis
 * Digestion of J23100
 * Transformation of ligated parts: J23106 (promoter), J06702 (RET), pSB4C5 (backbone)
 * Also Ligated: J23100 (promoter), J06702 (RET), pSB4C5 (backbone)
 * Plated, Controls and NEB 10 beta with BioBrick part (J23106, J06702, pSB4C5)

Trentay 20:26, 13 July 2009 (UTC)

Kevin and Adam Bussingkm 22:59, 13 July 2009 (UTC)
 * grew plasmids pSB4C5, E0240, and J06702 from glycerol stocks. Will be mini prepped in the morning.

Craig and Clay
 * The three parts related to limonene synthesis were electrotransformed again and plated on ampicillin plates.