Team:Brown/Notebook Meetings/8-10-09

iGEM Meeting 8-10-09

SFH 218

Attendees: Diana, Adrian


 * Team 1 update
 * Got EV131 into pNoTat
 * Growing in competent BL21
 * IPTG induction
 * Last year Team Resistance did an IPTG induction
 * Will do Western blot (Diana has 6-His antibody)


 * Team 2 update
 * Successfully cloned Agr operon promoter, moved into pGEM
 * Digested and ligated into Biobrick vector
 * Agr promoter + RFP growing on plates, will test quorum sensor
 * Electroporation failed; might be due to Tet plates, or faulty electrocompetent cells
 * Made Trg-EnvZ. Doing blue-white test
 * However, maybe did not distribute X-gal and IPTG evenly
 * Looger said he will get back this week about receptor
 * Running out of S. epi expression vector


 * Team 3 update
 * Ran SDS PAGE on Taz1, too much noise on protein gel
 * Tested mutagenic primers
 * Will send off for sequencing


 * Administrative stuff
 * Booked hotels for the Jamboree
 * We have 2 time slots at the Jamboree, may split up??