Team:Osaka/NOTES

NOTES

Calendar

Registry Parts All of Osaka's Parts and Devices is here COLOR K204033 mOrange placI K204041 placI+GFP K204042 placI+YFP K204043 placI+CFP K204044 placI+RFP K204048 placI+mOrange pcI K204058</a> pcI+YFP K204059</a> pcI+CFP K204062</a> pcI+mOrange ptetR K204052</a> ptetR+mOrange Carotenoid K204064</a> crtE+crtB K204065</a> crtI+crtY K204068</a> crtZ K204076</a> placI+crtE+crtB K204077</a> ptetR+crtE+crtB K204091</a> placI+crtE+crtB+crtI+crtY K204095</a> placI+crtE+crtB+crtZ K204096</a> ptetR+crtE+crtB+crtZ gradation K204069</a> cI repressor K204086</a> 3O6HSL receiver+RFP+cI repressor

SIGNAL </b> (Quorum Sensing) AI-1 K204047</a> LasI generater C4HSL K204040</a> RhlI (generater) K204031</a> RhlR (receiver) <a href="http://partsregistry.org/Part:BBa_K204702">K204702</a> RhlR+GFP 3OH,C14 : 1-HSL <a href="http://partsregistry.org/Part:BBa_K204025">K204025</a> cinI (generater) <a href="http://partsregistry.org/Part:BBa_K204032">K204032</a> cinR (receiver) 3O6HSL <a href="http://partsregistry.org/Part:BBa_K204051">K204051</a> LuxR (receiver) <a href="http://partsregistry.org/Part:BBa_K204700">K204700</a> LuxR+GFP

MOTILITY <a href="http://partsregistry.org/Part:BBa_K204018">K204018</a> ptetR+EspE <a href="http://partsregistry.org/Part:BBa_K204501">K204501</a> RBS+FliH <a href="http://partsregistry.org/Part:BBa_K204502">K204502</a> ptetR+FliH <a href="http://partsregistry.org/Part:BBa_K204503">K204503</a> pT7+FliH <a href="http://partsregistry.org/Part:BBa_K204504">K204504</a> C4HSL receiver +FliH

Lab Note Wet Lab

Week 1 : 8/2~8/8 Brainstorming, parts planning , prepared competent cell ransformation and miniprep from MIT plate week 2 : 8/9~8/15 start assembly parts (K204001 ~ K204006), transformation and mini prep from MIT Week 3 : 8/16~8/22 assembly parts (~K204015),  parts planning Week 4 : 8/23~8/29 assembly parts (~K204021) Week 5 : 8/30~9/5 assembly parts (~K204030) Week 6 : 9/6~9/12 assembly parts (~K204046) Week 7 : 9/13~9/19 assembly parts (~K204050) Week 8 : 9/20~9/26 assembly parts (~K204056) TEST : sensitivity of inducer and receptor Sample : 3O6HSL, C4HSL , AI-1 , 3OH,C14:1-HSL Result : Not confirm the fluorescence... Week 9 : 9/27~10/3 assembly parts (~K204066, X1~X8), color (fluorescence) intensity check Week 10 : 10/4~10/10 assembly parts (~K204078) Lux receiver sensitivity test, color (fluorescence) intensity check sequence check : generator and receiver 3O6HSL, C4HSL , AI-1 , 3OH,C14:1-HSL <h4 style="text-indent:2em">Get the parts from Team Chiba. Thanks a lot!! Week 11 : 10/11~10/17 assembly parts (~K2040104) sequence check : FliH etc… send Osaka team parts to MIT FINISH! <h4 style="text-indent:2em">Finally we assembled about 120 parts!! Dry Lab Week 5 : 8/30~9/5 Planing model. Week 6 : 9/6~9/12 Studying programing using Matlab. Week 7 : 9/13~9/19 Making program in case of single coloney. Week 8 : 9/20~9/26 Making program in case of more than two colonies Making movie file Searching paper written some parameter Week 9 : 9/27~10/3 Blash up our program Searching paper written some parameter Week 10 : 10/4~10/10 Making program of color gradation Week 11 : 10/11~10/17 Blash up our program Atelie Week 8 : 9/20~9/26 Making art works Week 9 : 9/27~10/3 Making art works Week 10 : 10/4~10/10 Making art works Week 11 : 10/11~10/17 Making art works

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