Team:Warsaw/Calendar-Main/5 September 2009

Acquiring the internaline A gene from Listeria monocytogenes st. EDG-e genome
Jarek

Tasks:
 * Electrophoretical segregation of PCR products from previous day in 0,8% agarosis gel


 * Preparation of another PCR reaction with L.monocytogenes genome DNA

Results:
 * I propably took the wrong genome DNA sample for the previous reaction, there are products but none of them has the correct length.

Assembly of endosomal detection operon Marcin Task 1:
 * Prepare the bacterial cultures for isolation of following constructs:
 * pSB1A3 plasmid containing cro coding sequence
 * pSB1A3 plasmid containing Bax coding sequence

Task 2: Methods: Plasmids were isolated using the A&A plasmid mini kit. Detailed procedure of the isolation is described here
 * Isolate the plasmids containing previously described constructs

Task 3: Methods: 2 &mu;l purified plasmid DNA product 0.5 &mu;l XbaI (Fermentas) 0.5 &mu;l PstI (Fermentas) 2 &mu;l Buffer Tango (Fermentas) 15 &mu;l MQ water Results:
 * Digest previously isolated plasmids and verify the correctness of the ligation:
 * Reaction mixture composition:
 * Digest was performed 90 minutes

Comments:
 * All samples of cro CDS on pSB1A3 are only empty plasmids
 * Samples of Bax CDS have biobricks but I am unable to determine whether this biobricks is Bax CDS or BBa_E0010 due to contamination with the latter one. It is obligated to prepare another digestion to reveal the identity of the insert

Bistable switch 1 testing Ania Tasks:    