August/31 August 2009

1. Miniprep of (12)-B, (13)-A, (14)-A, (15)-A, (16)-A, (19)-A solution cultures inoculated from streak plates on 8/30. Failed due to mistake in procedure; the above numbered parts were again inoculated from streak plates into culture solutions for miniprep tomorrow.

2. Ligation check of (12) through (16), (18) and (19) <- all miniprepped on 8/30; checking was done by taking a small sample for electrophoresis and length comparison. UP  : No.12, 15 ,16 , Vector , 13 , 14 , 18 , 19 , Vector , ladder DOWN : No.20-I,V, ladder , No.21-I,V

Results for (14), (18), (19) were not satisfactory; the others seemed okay and may be established as completed parts.

3. Repeat of ligation assembly of (20) and (21): today their constituent parts were treated with restriction enzymes, electrophoresed and purified. Tomorrow they will be treated with ligation enzymes followed by transformation.

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