September/23 September 2009

1. colony check sample no   no. of colony 22            100<     25             100<     40             100<     47             100<    X1-A            50< X2-B            2 X1-B            3 X3-B            2 X2-A            50< 1-12M           10< X3-A            10< 37             0     39              0

2.min prep result of nano drop sample no     conc. 1-2M         42 ng/uL 1-12M        61 X1-B         47 X2-A         98 38         234    X3-B          88 36A        247 36B         23 X2-B         96 X1-A         61 X3-A         64

3.digation

X4-A

K204052

K204029

K204037

K204039

K204046 ↓ 37°C, 2hr

4.trandfromation sample no 28, 30 , X2-A , X1-B

5.prepared Lb/Amp plate

6.digation

X5

X6

X7

X8

↓ 37°C, 2hr gel electrophoresis gel cut purification ↓ ligation

7.digation K204053

7.transfromation sample no. X4-A, X4-B , 52 29, 37 , 39 , 46 3-1M , 11

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