IBB Pune/27 June 2009

I) Results for yeaterday's tranformation.

CBG = Cohesive, Bangalore Genei BBG = Blunt, Bangalore Genei CP = Cohesive, Promega PCR


 * CBG
 * NEB 1 Colony
 * DH5a ~50 Colonies


 * BBG
 * NEB 2 Colonies
 * DH5a ~150 Colonies


 * CP
 * NEB 0 Colonies
 * ~70 Colonies


 * PCR
 * NEB 0 Colonies
 * DH5a ~130 Colonies

Hence, DH5a Rocks...

Also, PCR works.

II) Inoculated a single colony of this to LB+KAN for plasmid isolation.

III) Yesterdays PCR gave us only 2 bands, other than the expected range.. :-(

Why?
 * Wrong template?
 * Wrong ladder?

--rerun gel and rerun pcr--

III) Ran a test PCR of a-Mating Factor, p3w13d

IV) Double RE digest of Lycopene Cyclase.

Ran a gel.

Great gel, expected result.

V) Gel Eluted.


 * XP - 159ng/ul
 * SP - 56 ng/ul

kept for ligation.