Team:TorontoMaRSDiscovery/5 June 2009

=June 5, 2009=
 * 1) Tet plates made
 * Recipe for 200 ml (approx. 10 plates):
 * 2.2 g agar in 200 ml fresh LB
 * Note: do not re-autoclave LB, it will caramelize!
 * Recipe for 200 ml LB:
 * a) 1 g yeast extract
 * b) 2 g peptotryptone
 * c) 2 g NaCl
 * d) 200 ml water
 * 1) Autoclaved at 11:20 a.m., picked up at 1:15 p.m. and cooled in water bath at 37 degrees Celsius
 * 2) Added 200 microlitres tetracycline stock (from -20 freezer) to final concentration of 20 microgram/ml
 * 3) Swirl and poured into prepared, labeled plates
 * 4) *Note: label on plates reads June 3, 2009 but should be June 5, 2009 (8 plates in total - either I overpoured or these plates are a bit larger than I was used to)
 * 5) Inverted and put in 37 degree incubator to dry