Team:Paris/4 August 2009

NoteBook
August 4th

 

Brain work
A lot of meeting today: First on for the modeling explication and application of stochastic model ? The second one on every parts and each member's work explanation. We still arguing about the travel agency for the Jamboree...Waaaaaaaaa

 

Lab work
Overnight culture Biobrick resuspention Transformation of precited Biobrick Purification  
 * Keio Collection
 * S20 JW4251: LB Kan1000x 200rpm 37°C
 * S21 JN0729: LB Kan1000x 200rpm 37°C
 * S22 JN0728: LB Kan1000x 200rpm 37°C
 * S23 JN0727: LB Kan1000x 200rpm 37°C
 * S24 JN0940: LB Kan1000x 200rpm 37°C
 * S24 JN0940: LB Kan1000x 37°C
 * S25 JW5100: LB Kan1000x 200rpm 37°C
 * S25 JW5100: LB Kan1000x 37°C
 * S26 JN5181: LB Kan1000x 200rpm 37°C
 * S27 FF64: LB Tet1000x 200rpm 37°C
 * S28 NEC280: LB 200rpm 37°C
 * S5 MG4: LB Kan NaCl20% 200rpm 37°C
 * S6 DH5&alpha;: LB 200rpm 37°C
 * S24 JN0940: LBagar plate with Kan1000x 37°C
 * S25 JW5100: LBagar plate with Kan1000x 37°C
 * 1/1K named 1K1 (pSB1A3). Resistant to Ampicilin.
 * 1K1 (pSB1A3) in competent DH5&alpha; by RbCl
 * Purification of P3,P4,P5,P7 with XbaI PstI (using 1,5% agarose because pb is inferior to 100pb during 25 min)And P8 with (1%Agarose during 25 min because pb is superior to 1kpb).
 * D1=P3 Digested=66pb, D2=P4 Digested=95pb, D3=P5 Digested=37pb, D4=P7 Digested=54pb, D5=P8 Digested=1210pb, (D4 seems to be bad because we don't see his specific band during a gel after is purification)
 * P1 didn't work (we don't have the specific band after the digestion so we can't putificate it), P2 purification, for tomorrow
 * Gel for check the purification[Ladder 100bp|P3|P4|P5|P7|nothing|ladder 1kb|P8]
 * D1=Works, D2=Works, D3=Doesn't work(his band not correspond at 37pb), D4=Doesn't work(no band), D5=Work not well (2 bands)