Virginia Commonwealth/31 July 2009

Results
Maria and Afton Trentay 17:50, 31 July 2009 (UTC)
 * Overnight culture of I1352 grew, but no visible florescence
 * Transformation Results below
 * Interpretation of Transformation Results:
 * Shows the digestion/ligation process is not working
 * Because the I1352 grew successfully on an AMP plate after being 'ligated' with pSB4C5, shows that the original backbone is present
 * So it was not properly digested
 * No growth was visible on the CM plate with the ligated I1352 with the CM resistant backbone pSB4C5
 * If the digestion did not work properly, then the backbone could not be ligated with the I1352's promoter and RET
 * Also, all previous stocks of BioBricks with promoters (100-107,110,113,116,0011,0040) have successfully grown on CM resistance plates, meaning they have the correct backbone, but none express RFP.
 * Conclusion:
 * The enzymes are bad for digestion
 * The ligation process is a problem, because the original plasmid is going back into itself
 * A new kit is being ordered
 * Plans need to be made to repeat the Digestion and Ligation process with all promoters when kit arrives

Tasks
Afton and Maria

Wetlab
Afton and Maria