Imperial College London/Notebook/17 September 2009

Protocol considerations

 * Cells were grown overnight in 10ml of M9 media
 * The cells were allowed to grow until a high OD (very cloudy) and retrieved in the early afternoon
 * The OD was measured using the multiplate reader.
 * As the OD was only around 1.5, the cells were centrifuged and resuspended in less media to form an OD of 3
 * OD of around 0.5 to 3.0 was made up, and subsequently diluted up to 10-6 to 10-8
 * Plated on strep plates and dried until the end of the day
 * Placed into 37 degrees incubator (non-shaking) overnight

Results
The plates had too many colonies to count.

Conclusions

 * The plates were all too confluent, even at 10-8 dilution.
 * Unable to count colonies and draw any colonies
 * Need to repeat experiment

Suggestions/Improvements

 * Was not done on ice, so cell growth still occurred during dilutions
 * Too many colonies to count when plated 100uL
 * Use ice
 * Increase dilutions