EPF-Lausanne/10 July 2009

  

10 July 2009

Wet Lab
Miniprep of the yesterday transformations were done, glycerol stock of Inverter TetR (BBa_I6007) and Death Cassette (BBa_P1010) were done and finally they were put at -80°C.

As Prom_T7-RBS-CBP-LOVTAP PCR product migration on the gel didn't worked (in fact there wasn't enough products) July the 9th, a second PCR was done with more cycles (40) to check wether the the primers were accurately designed.

Results: The primers are correct -> Prom_T7-RBS-CBP-LOVTAP was correctly amplified.

A digestion-ligation according iGEM special protocol [[Media:‎ BioBrick_Assembly_Manual.pdf|Biobrick_Assembly_Manual]] was done with LacI and RBS. And Term was digested (with E and X) to linearize the plasmid.

As Inverter TetR (BBa_I6007) and Death Gene (BBa_P1010) were transformed with a contaminated SOC, we did a PCR with the isolated plasmids to check if the plasmids were correct.

Results: Death Cassette (BBa_P1010) sample contain the correct plasmid, Inverter TetR (BBa_I6007) doesn't.

Finally, a gel extraction was done to purify the digested Terminator (BBa_B0010) (linearized plasmid)

People in the lab

 * Heidi, Tu, Nath, Rafael, Basile

 