Team:Chiba/Notebook/Calendar/3 September 2009



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(2_September_2009 <|>4_September_2009)

Making Fluorescent Transfer Curve for the Practice of Lab Work (Day 2)
10:30 We took 30 mL of the liquid medium that we had made yesterday to conical flask and added prior culture solution 300 &mu;L, then started shake culture at 37 degrees Celsius.
 * main culture

We kept mixture of 300 &mu;L of 50% glycerol and 300 &mu;L of solution which is cultured yesterday in deep freezer.
 * Making glycerol stock

16:00
 * Making Transfer Curves

We put the solution that has completed main culture in 48 deep wells.

17:15

We shook 48 deep wells at 30 degrees Celsius.

While shaking, we measured OD and GFP fluorescence intensity of sample.

OD

GFP Fluorescence Intensity

18:30

We put the sample on plate and measured OD and GFP fluorescence intensity.

OD

GFP Fluorescence Intensity

19:24

In a similar way.

OD

GFP Fluorescence Intensity

20:23

In a similar way.

OD

GFP Fluorescence Intensity

21:18

In a similar way.

OD

GFP Fluorescence Intensity

22:56

We stopped this experiment.