Team:TUDelft/15 September 2009

=15 September 2009=

Sriram
Today I was able to do miniprep alone of the LacI generator and Normal RBS Control since I had meeting today.

Tim Weenink
None of yesterdays inoculations showed GFP expression. All aTc induced inoculations should have. So I did PCR reactions to check the lengths of the inserts.

These reactions were gelled:

After that I did replicaplating of all !Z plates on plates with Cam and Cam&aTc. This way I could screen for colonies that did express GFP when induced with aTc. But it later turned out that none of the colonies on the plate with aTc expressed GFP.