Team:Newcastle/Meetings/9 April 2009

Instructor Meeting 9th April 2009
Students: Jess, James, Matt, Craig, Hanny, Goxsel Instructors: Jen, Matthew, Neil

Apologies: Dan, Morgan, Jane, Arun

Actions
Neil - sin operon info to students, and BioBrick tutorial

Jen - circulate drawing of stochastic switch / slider

Students - email Neil about tutorial [done]

- consider the comments / notes and produce a priority plan of the way forward

- once finished send flow chart to Nigel Robinson for verification

- E-mail Colin Harwood to ask if there is an invertible sequence of Bacillus DNA

- Make a list of all the promoters that can be used in Bacillus so we have a list to pick from

- High priority:

1. update flow dia. and gene on / off table.

2. concentrate on some modelling priorities

3. Need to do a table of inputs (metal combos) and ouptputs (on / off - gate type)

4. Need to understand sporulation stages, genes etc... (see notes for more details)

BioBrick Parts
Metal Sensor

 - ! Need to do a table of inputs (metal combos) and ouptputs (on / off - gate type) !

- is arsR a epressor or activator ?

- re-do brick for sensor, promoters and correct order

 Stochastic Switch or Slider 

- control over sinR or sinI should give us a stochastic sliding effect like a fader on your hi fi - nice looking biobrick too!

- issues with complexity of the system oligermerization etc... model needed maybe Jane and Goxsel lead on this (Neil)?

- Jen will send round a re-drawm circuit

- ! Need complete flow diagram and pictures of sporulation cell cycle inc genes, factors, promoters, activators, repressors and everything else !Check it with Geoff Herrington.

- Protease tags in switch proteins ? Not sure what this means: was it so they get broken down quickly once they do the job?

 Metallothionein = Metal Mops

http://en.wikipedia.org/wiki/Metallothionein

- Getting into spore using tags - what is the consensus seq of these tags?

- Issues about when to switch on and off

-

Other Business
- Email Neil about BioBrick tutorial 30th April[done]

- Mike Cooley signed up as instructor

- SGM (Soc. General Microbiology) has donated funding

- Neil can send us some info on sin operon he has

- Neil and Alister (Edinburgh Uni.) discussed a UK get together in July

- Can we do something with small spore proteins to make them tougher?

Matt will be absent from the next meeting.