Team:Gaston Day School/10 July 2009

 Resuspend pelleted bacterial cells in 250x buffer P1 (kept at 4C) and transfer to a microcentrifuge tube Add 250x buffer P2 and gently invert the tube 4-6 times to mix Add 350x Buffer N2 and invert the tube immediately but gently 4-6 times Centrifuge for 10 min at 13k rpm (~17,900 xg) in a microcentrifuge Apply the supernatants from step 4 to the QIAprep spin column by decanting of pipetting Centrifuge for 30-60s. Discard the flow-through Wash QIA prep spini column by adding .75mL buffer PE and centrifuging for 60-70s Discard the flow through and centrifuge for and additional min to remove residual wash buffer</dt> Place the QIA prep column in a clean 1.5mL microcentrifuge tube. To elute DNA, add 50x Buffer EB (10mm onj.cl pH8-5) or water to the center of each QIA prep spin column. Let stand for 1 min. and centrifuge for 1 min.</dt>