Team:Gaston Day School/16 June 2009

 Spin tubes #1-8 for 3 minutes Combined tubes #1-4 and tubes #5-8 Added 300x TENS Mix by inversion Added 150x sodium acetate Mix by inversion Spin for 2.5 minutes Transferred Sup to clean tube</dt> Added 1mL of EtOH (ethanol)</dt> Spin for 5 minutes</dt> Wasted pellet in 70% EtOH 300x</dt> Spin for 5 minutes</dt> Resuspend 30x TE</dt> Digest</dt> 20x DNA</dt> 3x Buffer</dt> 5x dH2O</dt> 2x EcoRI</dt>