Team:Chiba/Notebook/Calendar/21 September 2009



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(20_September_2009 <|>22_September_2009)

Transformation(1)-1
21:00

We Picked Biobrick parts.

BBa_E2060(mCherry)

BBa_T9003(LacZ alpha protein generator)

21:10

We introduced each plasmids into competent cells.

E2060, T9003 --> Cm resistant competent cells

pCIA3-LuxR and plux-GFP, plux-GFP only --> K resistant competent cells

21:30

We added 120 &mu;L of SOC medium each sample and cultured these at 37 degrees Celsius.

22:30

We planted each sample and cultured these at 37 degrees Celsius.

To judge character of LuxR mutants (1)-1
We poured 1 mL of LB-Amp, Cm liquid medium in 96 deep well and added glycerol stocks.

22:20-

We cultured and shook it at 37 degrees Celsius.