September/2 September 2009

1.taranfromation ligation sample No. 5, 9 , 18 , 20 , 21

2.digestion and ligation

digestion with restriction enzyme

K204018

K204022

K204023

K204025

K204026

↓ 37°C, 2hr ↓ gel electrophoresis

UP  : 18-I,V, 22-I,V , 23-I,V , 25-I,V DOWN : 26-I,V

gel cut purification by [QIAquick Nucleotide Removal Kit] ↓ ligation

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