Team:Chiba/Notebook/Calendar/29 September 2009



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(28_September_2009 <|>30_September_2009)

Gel electrophoresis
Yesterday's oparation is here.

10:40-
 * Today's operation

Mini Prep.

13:30-

Gel electrophoresis(135 V, 27 min)

---DNA Clean & Concentrator---

15:50-

Gel electrophoresis again(135 V, 27 min)

Digestion Test
Vector(plux-GFP)
 * Sample

Insert(sfGFP)


 * Vector


 * Insert

18:55-

Gel electrophoresis(135 V, 25 min)

19:45

Took a picture

Transformation
Competent Cells : XL10G(K)
 * Sample

Plasmid : plux-GFP(Cm)

21:45-

CmのプレートにまいてCultured it at 37 degrees Celsius.

Digestion
Vector : plux-GFP(p15A)
 * Sample

Insert : sfGFP

Enzymes : Nco1, BamH1

21:17

We added Enzymes and took it 37 degrees Celsius.

---3 h---

We took it 65 degrees Celsius for 酵素を失活させる

---20 min---

24:42

We kept it in refrigerator.

PCR
plux-HSUTK
 * Template


 * Element of mixtures