Team:Washington/Notebook/protein gel

Protein Gel

 * 1) Set up overnights of parts J36848-J36851
 * 2) Dilute 1&mu;L overnight into 1mL broth
 * 3) Add 1 mM IPTG and let grow for four hours
 * 4) After cells have grown up to saturation, heat water to a boil
 * 5) Add 100&mu;L of overnight to a 1.5mL tube
 * 6) Pellet by spinning at max speed for 30 secs in the microcentrifuge
 * 7) Discard supernatant
 * 8) Pull an aliquot of 5x sample loading buffer out of the freezer and thaw
 * 9) Add 20uL BME to aliquot
 * 10) Resuspend samples in 50&mu;L sample loading buffer (pipette up/down)
 * 11) Boil samples for 10 minutes
 * 12) While boiling, prepare 500mL 1x SDS buffer:
 * 13) 50mL 10x buffer to 450mL water
 * 14) Take a gel out of the fridge and and put it in the gel box (keep the gel container for staining!!!)
 * 15) Pour the mixed buffer solution into the half of the gel box that the gel is in
 * 16) Remove the gel comb
 * 17) Fill the little container on the top of the gel until it's about 0.5 cm from the top with buffer
 * 18) Remove any bubbles in the wells
 * 19) Vortex samples
 * 20) Spin down samples for a few seconds
 * 21) Load 3&mu;L into each well
 * 22) Load 10&mu;L of ladder in appropriate wells
 * 23) Run at 180V until the dye is about to fall off the gel