Wisconsin-Madison/12 June 2009

June 12, 2009
Ex 11: Salt tolerance of E.coli DH10B:

Use plate reading machine to chart OD over time

ExA:	30uL of DH10B ecoli, 200uL of LB

OD taken every 30 min for 48 hours

Could not start today because Rebecca stole our Plate reader time slot

Ex 10: Made BG-11 plates

Other: Induced nud F

Freezer stock

Transformation of spinach cdna success.

- received the order of primers (diluted 10uL/nmol) - NP - remade freezer stocks of pSB1A3, pRL1383A, and pMMBRraA - NP - induced nudF cultures - NP - did boiling lysis mini preparation of pRL1383A - NP