Wisconsin-Madison/4 June 2009

June 4, 2009
Other: Meeting

Worked on Website

Ex 6 Results: No good, past optimum optical density

Need more samples

Made more inducible cultures: (IPTG)

1. Sample 1: 100ml LB,50uL Amp, 73.5uL Cm, 100uL Tet : Triple Transformation Induced with +50uL IPTG 1.5 g Arabinos

2. Sample: 100ml LB  50uL Amp   73.5uL Cm, 100uL Tet : Triple Transformation Let sit for overnight

3. Control: 100ml LB, 50uL Amp, 73.5 Cm : Empty Plasmids 33+99

Ex 5: Plated Triple on Amp, Cm, Tet Plate from Freezer for Test

Other:

Made Antibiotics from Streptomycin

Made plates with Streptomycin

Nate got new valve and pipe for autoclave and it works now

- induced a pNudF, pMBI, pMevT culture with IPTG and arabinose (unsuccessful) - NP