August/30 August 2009

1. ligation rapid check

UP  : 13-A,B,C ,  14-A,B,C  , 19-A,B,C  , vector ,ladder DOWN : 12-A,B,C,  15-A,B,C  , 16-A,B,C  , vector , ladder

No.13 (RBS+rhlI),K204013 13-A,C : OK

No.14 (RBS+LasI),K204014 14 -A,C : OK

No.19 (RBS+RFP(orange?)),K204019 19-A,B : OK

No.12 (RBS+cinR+terminater),K204012 12-B : OK ?

No.15 (RBS+cinI+terminater), K204015 15-A,B,C : OK?

No.16 (RBS+LuxI+terminater), K204016 16-A,B,C ok?

2.digestion and ligation

digestion with restriction enzyme

K204020

K204021

3.12-B, 13-A , 14-A , 15-A , 16-A , 19-A  were inoculated into growth culture solution. Miniprep should be conducted on the resulting cell cultures tomorrow.

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