September/15 September 2009

1.colony check

sample No. no. of colony 14            +++    17              0    47              +    48              1   2-24I           ++ 45             0    46              0   1-14N           +++

2.ligation

K204045

K204046

34-I,V, 45-I,V , 46-I,V

UP  : 20-I,V, 27-I,V , 28-I,V , ladder DOWN : 29-I,V, 30-I,V , 35-I,V , ladder

gel cut purification ligation 16degree O/N

3.transfromation

1-1D, No.19 , 2-18F No.17, 45 , 46 , 37 , 38 , 1-2M , 1-8I

for DNA modification No.41, 42 , 18 , 1-1K , 1-16I

back to NOTES