Team:TorontoMaRSDiscovery/4 September 2009

=September 4, 2009=
 * 1) Miniprepped overnight cultures
 * 2) stocked Amp, K1, K2, and Enc+5 (2), (3), (4)
 * 3) Digested Enc+5 (1)-(4), K1,2, BB7
 * 4) Transfected 1+2+3+2 into DH-5 cells (50ul)
 * 5) *left on ice for 2.5h after adding ligation product
 * 6) *incubated on shaker for about 2h
 * 7) Started overnight of C plasmid (ccdb)