Virginia Commonwealth/30 July 2009

Results
Maria and Afton
 * The overnight culture grew, however, no RFP was expressed (I1352)
 * Spectrophotometry measurements


 * Gel Electrophoresis Results

Trentay 14:26, 31 July 2009 (UTC)

Tasks
Maria and Afton Trentay 14:26, 31 July 2009 (UTC)
 * Make Cryogenic Stocks of part I1352 (2 vials)
 * Make another overnight culture from the previous culture to see if it will express RFP
 * Miniprep I1352
 * Take spectrophotometry measurements of I1352 and pSB4C5
 * Digest and Ligate I1352 and pSB4C5
 * Run a Gel Electrophoresis on pSB4C5 and I1352 using the Hyper Ladder and the 100bp ladder
 * Transform parts into NEB 10 beta

Wetlab
Maria and Afton Trentay 15:10, 31 July 2009 (UTC)
 * Cryogenic stocks were made
 * Overnight culture of I1352 was grown up in 5mL of LB+AMP
 * 2 samples of 1.5mL of I1352 was Miniprepped
 * Spectophotometry measurements were taken
 * The two samples were combined into one tube before measurements were taken
 * pSB4C5 that was miniprepped on July 27, 2009
 * Digestion was done
 * Only the restriction enzymes EcoRI-HF and Pstl were used to cut the separate the backbone from the promter and RET
 * Ligation was done
 * for the Ligation of I1352 with pSB4C5, 3 microliters was added of each digestion to the Ligation sample
 * for the Ligation of I1352 with itself, 6 microliters of the Digestion was addes to the Ligation sample
 * Gel Electrophoresis was run at 120v for 45 min and DNA bands appeared for both samples
 * Transformation was done on the two ligated samples