Team:Brown/Notebook Meetings/6-16-09

Team Tasks- June 16, 2009


 * Team 1: EV131- Indu, Ahmad, Minoo
 * Team 2: S.aureus- Eli, Flora, Will
 * Team 3: Bistable toggle- Michael, Ashley, Steph


 * Tasks on hand:


 * Team 1:
 * Posted on google groups
 * Transformation done→ need to grow them up
 * Ordered primers
 * Sequence protein?
 * Sit down and write down protocols
 * Put it in pnotat vector; transform
 * Affinity tests- Keq


 * Team 2:
 * S.aureus→ go to biosafety, find strain, lab regulators today: 164 Angell Street
 * TJ Foster→ email reg. signaling tag
 * Talk to labs that have used S.aureus
 * Quorum kill switch
 * Directed evolution of histamine signal transduction prokaryotic gram+ system
 * Encapsulation


 * Team 3:
 * BioBricks are in registry except Pxyl/TetO→ contact TJ Foster (check for sequence)
 * Construction of model circuit
 * Read Collins paper (methods!!!)