User:DavidC/23 August 2009

Sunday the 23th
Lambda phage extraction.

PCR
Sample: D protein.

Cycle :

94°C 2minutes; (94°C 1 minute; 59°C 1 minutes; 72°C 1 minute(s)) X 35; 72°C 4 minutes.

Samples: Adenovirus 5, penton base.

Cycle :

94°C 2minutes; (94°C 1 minute; 62°C 1 minutes; 72°C 3 minute(s)) X 35; 72°C 6 minutes.

Samples: COS sequence from Lambda phage.

Cycle :

94°C 2minutes; (94°C 1 minute; 57°C 1 minutes; 72°C 1 minute(s)) X 35; 72°C 4 minutes.

Samples: CI repressor.

Cycle :

94°C 2minutes; (94°C 1 minute; 57°C 1 minutes; 72°C 3 minute(s)) X 35; 72°C 6 minutes.

DNA electrophoresis
85 Volt, 15 minutes. 105 Volt, 40 minutes. Ladder fermentas 1 Kb.

Samples: D protein (D prt)385bp, adenovirus penton base (ADV5pb) 1725bp, COS sequence 250bp and CI repressor 713bp.