Duke University/10 June 2009

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June 10, 2009

 * E: Gel to check PCR
 * R: Smears at bottom of gel
 * C: Should use Bioplastics #1 as template to increase amount of fragment 1 and Bioplastics #3 as template to increase amount of fragment


 * E: PCR with Bioplastics #1 and #3 as templates and doubled volume, Gel to obtain more of fragments
 * R: Smears for fragment 1, Correct bands for fragment 2, Correct bands and smears for fragment 3
 * C: PCR with different conditions


 * E: PCR with Bioplastics #3 as template, extension time increased to 1m 30s, cycles increased to 40