Wisconsin-Madison/16 July 2009

July 16, 2009
Other: 

Advisor Meeting (only Jenny)

Faxing

Research

Waiting for Primers

Ex 22: Triple Induction

Growth: Transformation of Double - Triple: good

(pic here)

Inoculate: 

1.	Triple: DH1 – Terrific Broth

2.	Triple: DH1 – M9 Media

Double Digestion of BB (X+P)

1.	NudF (560+2157=) 2717 bp

2.	Choline (2000+2157=) 4157 bp

3.	BB (2157+1069=) 3226 bp control

F F1 F2 C

Ex 18: Taq

Chris ran PCR with taq protein – YhfR

If Fails, Taq fault, we have YhfR as BioBrick