Team:Imperial College London/Wetlab/Protocols/Cellculture/LBPlates

=Make LB agar plates + antibiotic=

Day 1
 Make LB agar |How to Link Send LB agar for autoclaving (before 12.30) 

Day 2
 Get LB agar from autoclave Switch water bath to 50C Microwave LB agar flask until agar dissolves Put the LB agar flask into the water bath for 20-30 mins (this ensures the agar is dissolved properly) Turn on the Bunsen burner. From now on work next to the flame for sterile technique.</li> 5 minutes before pipetting (see next step), take antibiotics out of the freezer and let them defrost</li> When antibiotics have defrosted, add 200μL of antibiotic to 200mL of LB agar. Ratio of AB:LB is 1:1000</li> </ol>