Team:EPF-Lausanne/Protocols/Transformation

 

Transformation

Material

Procedure
 * 50 µl competent cells
 * LB-agar plates with corresponding antibiotics
 * Water bath at 42°C
 * 1. Thaw cells on ice for 20min
 * 2. Add DNA (ligation product) to the cells, mix.
 * 3. Incubate 20min on ice
 * 4. Heat shock 45s @ 42°C
 * 5. Incubate 20min on ice (optional)
 * 6. Add 500 µl SOC and incubate 1h @ 37°C (shaking)
 * 7. Spread over LB-agar plate
 * 8. Incubate overnight @ 37°C