EPF-Lausanne/24 August 2009

  

24 August 2009

Cultures
25ml of each of the following one with LB and antibiotic:
 * Read out 2 (RO2) #4
 * RO2 #5
 * RO2 #10

-> cells were taken from the plates

Cells were then incubated at 37°C during approx 3h, we then measured their optical density (OD). These are the results:
 * #4 : 0.14
 * #5 : 0.08
 * #10 : 0.14

They are all stored in the fridge at 4°C

Cultures overnight for miniprep the next morning, to send the plasmid for sequencing.
 * 3x5ml LacI-RBS #2
 * 3x5ml LacI-RBS #5
 * 3x5ml LacI-RBS #7
 * 3x5ml Read out 2 #5
 * 3x5ml LovTap-term #5
 * 3x5ml BB1 (LT 1.6-LR 5 (PCR) n°13)
 * 3x5ml BB3 (LT 2.2-LR 2 (PCR) n°2)
 * 3x5ml BB5 (LT 2.12-LR 7 (PCR) n°1)
 * 3x5ml BB6 (LT 1.6-LR 7 n°2)

For the other plasmids, we still have sufficient volums and concentrations already "minipreped" in the freezer at -20°C.

People in the lab
Basile, Rafael, Nicolas

 