Team:Paris/Protocols transformation

Protocol for bacterial transformation

 * 250µl of DH5α competent bacteria (by RbCl)
 * Add 2µl of DNA (ex Biobrick)
 * Incubation during 20min on ice
 * Heat choc 45second at 42°C
 * Leave on ice 2min
 * Add 1ml of LB preheated at 42°C
 * Incubation 1 hour at 37°C under agitation
 * Put 100µl of culture under plate
 * Centrifuge 2min at 6rpm MAX for 2 min
 * Put 100µl of concentrated culture
 * Incubate overnight at 37°C