August/25 August 2009

1.trandformation

ligation sample No. 12 ,13, 14 , 15 ↓ 65°C, 10min ↓ competent cell 100uL sample    10uL competent cell 100uL sample     2uL

2.Min prep Result of Nanodrop concentration check (sample No.) (concentration) 1-6I        13.5 19.1                    15.4                     13.4                     18.9     1-4E         15.0 15.3                    14.9     2-16N        22.7 17.7                    23.1     2-16H        13.8 22.7    2-16L        22.5 31.5

3.ligation check sample No. 9, 10

4.digestion digestion with restriction enzyme

K204019

K204020

K204021

↓ 37°C, over night

back to NOTES