Team:IPN-UNAM-Mexico/Modeling/SecondAp

[[Image:Month-icon.png | 50px]]Second approach: Activator-Inhibitor dynamics on a single cell
Based on the design of the biobricks network we tried to model the behavior it would have within a single cell. To do this we constructed the kinetic laws describing the relationships between the components of our network and IPTG and aTc. We modeled the interactions of the AHLs with their respective targets as allosteric regulators affecting the production rate of the catalyzing enzymes, thus affecting the synthesis of the AHLs. This catalysis was assumed to be a first order kinetics depending only on the concentration of the catalyst.

The diffusion rate of the AHLs was reduced to a first order kinetics considering the membrane area of the bacteria and assuming the complete absence of the AHL outside the cell, therefore depending only on the production within the cell.

The integrated system


To build up the entire model we put together all the previous submodels, adding the diffusion of the AHLs and introducing a reporter moduel under the same regulation as lasI.

This system was built on Cell Designer software to explicitly denoting all the described interactions. Each coefficient of the kinetic rules was gathered from the literature and past years team wikis.

For this model. We tried to be very accurate on the description of our system, and the parameters and the kinetic laws we used are described below.