Team:NCTU Formosa/Notebook/Calendar

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Home  Team  Team Gallery</a></li> </ul> </li> </ul>

Project</a> <div class="select_sub"> Abstract</a></li> Introduction</a></li> Design</a></li> Parts</a></li> New Idea</a></li> Safety</a></li> </ul> </li> </ul>

<a>Results</a> <div class="select_sub"> <li><a href="http://2009.igem.org/Team:NCTU_Formosa/WetLab/Labworks" target="_self">Lab works</a></li> <li><a href="http://2009.igem.org/Team:NCTU_Formosa/WetLab/Timer" target="_self">Timer</a></li> <li><a href="http://2009.igem.org/Team:NCTU_Formosa/WetLab/Counter" target="_self">Counter</a></li> <li><a href="http://2009.igem.org/Team:NCTU_Formosa/WetLab/Memory" target="_self">Memory</a></li> <li><a href="http://2009.igem.org/Team:NCTU_Formosa/WetLab/Results_new" target="_self">New Idea</a></li> </ul> </li> </ul> <li><a href="http://2009.igem.org/Team:NCTU_Formosa/Modeling" target="_self">Modeling</a></li> </ul> <li><a>Notebook</a> <div class="select_sub"> <li><a href="http://2009.igem.org/Team:NCTU_Formosa/Notebook/Calendar" target="_self">Calendar</a></li> </ul> </li> </ul> <li><a href="http://2009.igem.org/Team:NCTU_Formosa/SubmittedParts" target="_self">Submitted Parts</a> </li> </ul> <li><a href="http://2009.igem.org/Team:NCTU_Formosa/Contact" target="_self">Contact</a> </li> </ul>

<a href="#jul" target="_self"> 7/15</a> <a href="#jul" target="_self"> 7/17</a> <a href="#jul" target="_self"> 7/20</a> <a href="#jul" target="_self"> 7/22</a> <a href="#jul" target="_self"> 7/27</a> <a href="#jul" target="_self"> 7/29</a> <a href="#jul" target="_self"> 7/31</a> <a href="#aug" target="_self"> 8/03</a> <a href="#aug" target="_self"> 8/05</a> <a href="#aug" target="_self"> 8/07</a> <a href="#aug" target="_self"> 8/10</a> <a href="#aug" target="_self"> 8/12</a> <a href="#aug" target="_self"> 8/14</a> <a href="#aug" target="_self"> 8/17</a> <a href="#aug" target="_self"> 8/21</a> <a href="#aug" target="_self"> 8/28</a>

<a href="#sep" target="_self"> 9/04</a>

<a href="#sep" target="_self"> 9/06</a> <a href="#sep" target="_self"> 9/07</a> <a href="#sep" target="_self"> 9/08</a> <a href="#sep" target="_self"> 9/09</a> <a href="#sep" target="_self"> 9/09</a> <a href="#sep" target="_self"> 9/10</a> <a href="#oct" target="_self"> 10/12</a> <a href="#oct" target="_self"> 10/14</a> <a href="#oct" target="_self"> 10/18</a>

</a> July <a name="0715" id="7/15"></a> 7/15 It is the beginning today!!!!!!!! <a name="0717" id="7/17"></a> 7/17 Lesson：Parts, Devices and Biobricks. In this lesson we learned about the principle of the construction of biobricks. <a name="0720" id="7/20"></a> 7/20~22 To present 2008 IGEM projects. <a name="0727" id="7/27"></a> 7/27~29 To present everyone’s primary idea. </a> August <a name="0803" id="8/03"></a> 8/03 A group duscussion of each one's personal idea. <a name="0805" id="8/05"></a> 8/05 To collate everyone’s advantages. <a name="0807" id="8/07"></a> 8/07 A group discussion. We decided what kind of projects we're going to construct. <a name="0812" id="8/12"></a> 8/12 A group discussion. We discussed about the principle and some details of the project. We also designed the first step of the experimence and the protocols. <a name="0817" id="8/17"></a> 8/17 Today we devided our team into 5 subgroups and started the experimence. In each subgroup, we have to contribute a part of the whole project. <a name="0818" id="8/18"></a> 8/18 We transformed the bricks we need from the well. <a name="0821" id="8/21"></a> 8/21 we check DNA by agarose gel, SDS-PAGE. <a name="0822" id="8/22"></a> 8/22~23 To transfer E. coli to new LB broth, and incubate for 16hr, then Extract plasmid. <a name="0828" id="8/28"></a> 8/28 we design a logo of team NCTU_FORMOSA </a> September <a name="0901" id="9/1"></a> 9/1 The experiment is not so smoothly, we sink into infinite loop in the same experiments. <a name="0902" id="9/2"></a> 9/2 To digest the front part (8/23) and the behind part (8/26) of the biobrick, ligate, transform and run gel to check that if digestion is OK or not, then spread on the plate, and incubate for 20hr. <a name="0907" id="9/7"></a> 9/7 We use another enzyme to digest to double check if the plasmid is right or not <a name="0910" id="9/10"></a> 9/10 Today we have a meeting that we discuss how to solve the problems. <a name="0914" id="9/14"></a> 9/14~25 The plasmid's sequences are wrong and we repeat the digestion, ligation, and transform again. <a name="0916" id="9/16"></a> 9/16 BBa_K188343 is sequenced today. <a name="0928" id="9/28"></a> 9/28 Degenerated PCR, using BBa_K145279 as template <a name="0929" id="9/29"></a> 9/29 Degenerated PCR

</a> October <a name="1002" id="10/2"></a> 10/2 Degenerated PCR. Restriction enzyme map. <a name="1007" id="10/7"></a> 10/7 Gel purification. To ligate gel purification products <a name="1011" id="10/11"></a> 10/11 to make restriction enzyme map. To select 14 plasmids <a name="1012" id="10/12"></a> 10/12 We had a team work to work on the wiki web. Flow cytometer. <a name="1014" id="10/14"></a> 10/14 Submitted the parts to be sequenced. <a name="1018" id="10/18"></a> 10/18 Updated the web of notebook and the project. We also had a group discussion about our results and the data. <a name="1021" id="10/21"></a> 10/21 To discuss our final result.