Team:PKU Beijing/Notebook/AND Gate 1/Input/TetR 2

Notebook > AND Gate 1 > Input > aTc Sensor

2009.8.16
Test if the tetR promoter system works well in the low-copy backbone. Get tetR+promoter+GRP from Wu Shuke.

16:40 Digest the promoter and reporter system.

20:00 Electrophoresis to recycle the inserts. The order of the samples: marker, digestion products, plasmids control. Results: Only the first one is correctly digested, recycle them.

2009.8.17
10:00 Link the inserts with vectors with has Kanamycin resistance.

17:50 Transformation.

19:30 Start to incubate.

2009.8.18
10:00 There are many colonies on the plate. PCR colonies to test if they are correct.

2009.8.19
9:30 Mniprep the plasmids. The general concentration of the plasmids are about 150ng/μL. 11:00 Digest and PCR those plasmids to test if they are correct. The digestion system:

The PCR system: 12:00 Start to digest&PCR. 16:30 Electrophoresis to test the digestion and PCR products. All the clones have correct colonies.

17:00 Induce the strain containing tetR and low-copy backbone by aTc. 22:00 Using flow cytometry to test the induction results. There are about 5 folds between the induced sample and the uninduced one.