Team:Gaston Day School/6 May 2009

 Transform pNICE ~ E. coli/dt> 50x competent cells on ice (Carolina Biol.) 200x cold 50mM CaCl2 10x 50ml pNICE (Lindow lab) Ice 15' 8:11--> Heat shock 42C 90" Add 250x LB 1 hour 37C Plate 100x, 100,x 200x, 200-250x</dt> Overnight 37C</dt>