Team:IBB Pune/problem the



Synthetic biology is demanding, both for humans and the bugs.Since the first iGEM, a lot of teams have attempted at solving various problems by designing big constructs that simulate the problem correctly, but often don't work because of the imposed metabolic burden on the microbe. Longer construct also means difficulty to transform and sequence. How can we make these large genetic circuits more easy on the bugs?

We try to solve this problem by 1:Breaking up the system into modules situated into different strains that interact with each other. 2.Simplifying the construct itself in such a way that the phenotypic output remains the same.

To test the effectiveness of these approaches, We have designed a construct to simulate a "unary adder-Turing machine", which is a simple three state Turing machine. Although the Unary adder is a very simple machine, the genetic construct is a whopping ~6kb chunk of DNA.