Team:Chiba/Notebook/Calendar/30 September 2009



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(29_September_2009 <|>1_October_2009)

Transformation
Yesterday's operation is here.

10:30-
 * Today's operation

Culture at 37 degrees Celsius

20:00-

Mini Prep.

20:40-

Digestion Test

20:56-

At 37 degrees Celsius

21:45-

Gel electro...(135 V, 25 min)

PCR
Yesterday's operation is here.

11:15-

Gel electro...(135 V, 27 min)

12:10

Took a picture

Digestion
Yesterday's operation is here.

12:37-

Gel electro...(100 V, 40 min)

13:20

into ethyl bromide

13:35

Took a picture and cut the gel.

Then we added 2 mL of ADB Buffer and kept it at 37 degrees Celsius.

DNA Clean & Concentrator
We eluted it 50 uL of dH2O.

DNA Clean & Concentrator
We eluted it 50 uL of dH2O.

Gel electrophoresis
17:32

Electrophoresis

18:15

Took a picture

Ligation
18:30

@Room Temperture

21:30

Transformation

21:50

@37 degrees Celsius

23:10

Plating