Uppsala-Sweden/13 October 2009

Evaluating transformation
One colony were picked for PRKRYT on XAK, inoculated and validated through a colony PCR.

Antisense RNA
The antisense RNA constructs growing on BG-11 plates without antibiotics were moved to selective plates with kanamycin and put in the light hood at 30 deg C.

Transformation
The failed antisense RNA p-E1B3-T were cut (E+P) again together with PRKRYT, PRPRYT and PRPRAT.