Team:BIOTEC Dresden/Notebook1-2

12th August:

1. Induction of fosmid has been done with L-arabinose to increase the copy number.

2. Induction of GB05MN cells with L-rhamnose has been done to increase the copy number.

13th August:

1. Glycerol stocks of GB05MN, EPI300, GB05(pccFos-MeCP2-Bsd), GB05MN(pccFos-MeCP2-Gent-rpsL) are made.

2. Clones of pccFos-MeCP2-Bsd and pccFos-MeCP2-Gent-rpsLare inoculated in LB-Cm10 with L-arabinose for induction overnight.

14th August:

1. DNA isolation of pccFosMeCP2-Bsd and pccFosMeCP2-Gent-rpsL have been done.

2. Sample DNA from both preparations are run on a gel for checking.