Wisconsin-Madison/28 May 2009

May 28, 2009
Other: Meeting with Brian

Ex 4:

MiniPrep of pMevT, MpBI, pMBIS

Ran gel

Made freezer stock



Spec DNA to determine concentration:

Ex 5: Ecoli Transformations:

1. Double transformation of NudF and MevT …plated on Cm, Amp

2. Triple transformation of NudF, MevT, and MBI… plated on Cm, Amp, Tet 3. control: Double transformation of pBAD33 and pTrc99A plated on Cm, Amp

- mini preparation of pMevT, pMBIS, pMBI - NP - made freezer stocks of pMevT, pMBIS, pMBI - NP - tested the concentration of the DNA with a spectrophotometer - NP - ran a gel of pMevT, pMBIS, pMBI (successful) - NP - transformed competent cells with pMevT, pMBI, and pNudF (all three in one and another with pMevT and pNudF only, only the double was successful) - NP