Team:Southampton/Project/Protocols/DoubleDigestion

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">   University of Southampton Wiki    /*TOOLBAR*/ position:absolute; left:-2px; top:0px; width:978px; height:27px; z-index:100; } /*FOOTERPUSH*/ width:1px; height:1217px; } html, body { margin: 0; padding: 0; } /*LAYOUT BEGINS*/ width: 95%; } position:absolute; left:0px; top:296px; width:975px; height:1000px; background-image:url('http://www.personal.soton.ac.uk/jeh2v07/images/bg.gif'); background-repeat:repeat-y; margin: 0; padding: 0; }	position:absolute; left:113px; top:1265px; z-index:150; color: #333; }	position:absolute; left:0px; top:1235px; width:975px; height:61px; z-index:100; } position:absolute; left:260px; top:320px; z-index:10; }	position:absolute; left:17px; top:100px; width:940px; height:800px; z-index:100; }
 * 1) toolbar {
 * 1) footerpush {
 * 1) notebooktable {
 * 1) maincont{
 * 1) sufooter {
 * 1) contfoot {
 * 1) bluesubtitle {
 * 1) pdf {

   Home  Project <![if gt IE 6]><![endif]>  <li class=" cssMenui">Project Aims</a></li> <li class=" cssMenui">Project Introduction</a></li> <li class=" cssMenui">Project Description</a></li> <li class=" cssMenui">Project Achievements</a></li> <li class=" cssMenui">Project Projection</a></li> <li class=" cssMenui">Project Safety</a></li> <li class=" cssMenui">References and Links</a></li> <li class=" cssMenui"> Protocols <![if gt IE 6]></a><![endif]> <ul class=" cssMenum"> <li class=" cssMenui">Protocol Overview</a></li> <li class=" cssMenui">Protocol Theory</a></li> <li class=" cssMenui">Biobrick Transformation</a></li> <li class=" cssMenui">Colony PCR</a></li> <li class=" cssMenui">DNA Double Digestion</a></li> <li class=" cssMenui">Gel Electrophoresis</a></li> <li class=" cssMenui">High Fidelity PCR</a></li> <li class=" cssMenui">LB Plates</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Project/Protocols/Ligation">Ligation</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Project/Protocols/Ligationtransformation">Ligation Transformation</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Project/Protocols/PlasmidPrep">Plasmid Preparation</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Project/Protocols/TA">TA Cloning</a></li> </ul> </li> </ul> </li> <li class=" cssMenui"><a class=" cssMenui" href="#">Biobrick Parts<![if gt IE 6]></a><![endif]> <ul class=" cssMenum"> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Parts/GoL">Game of Life</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Parts/RPS">Rock Paper Scissors</a></li> </ul></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Team"> Team <![if gt IE 6]></a><![endif]> <ul class=" cssMenum"> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Team">Team Profiles</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Team/Advisors">Advisor Profiles</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Team/Southampton">Southampton Profile</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Team/University">University Profile</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Team/Gallery">Gallery</a></li> </ul> </li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Modeling"> Modelling <![if gt IE 6]></a><![endif]> <ul class=" cssMenum"> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Modeling">Modelling Overview</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Modeling/GoL">Game Of Life</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Modeling/RPS">Rock Paper Scissors</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Modeling/Open">Open Access</a></li> </ul> </li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/HP"> Human Practices <![if gt IE 6]></a><![endif]> <ul class=" cssMenum"> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/HP">Overview</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/HP/Outreach">Outreach</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/HP/igemexper">iGEM Experience</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/HP/Political">Political Awareness</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/HP/Legacy">Our Legacy</a></li> </ul> </li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Notebook">Notebook</a><![endif]> <ul class=" cssMenum">

<li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Notebook">Notebook</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Notebook/Labbook">Lab Book</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Notebook/Advisorblog">Advisor Blog</a></li> </ul> </li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Sponsors"> Acknowledgments <![if gt IE 6]></a><![endif]> <ul class=" cssMenum"> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Sponsors">Sponsors</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Southampton">Team Southampton</a></li> </ul> </li> </ul> <img style="width: 975px; height: 24px;" id="bg" src="http://www.personal.soton.ac.uk/jeh2v07/images/maintop.gif" alt="" /> <img style="width: 63px; height: 63px;" alt="" src="http://www.personal.soton.ac.uk/jeh2v07/images/logobox.jpg" /> Protocol DNA Double Digestion <object data='http://www.personal.soton.ac.uk/jeh2v07/protocols/doubledigest.pdf' type='application/pdf' width='100%' height='100%'>

It appears your Web browser is not configured to display PDF files. No worries, just <a href='http://www.personal.soton.ac.uk/jeh2v07/protocols/doubledigest.pdf'>click here to download the PDF file.<a>

| <a href="#">Top</a> | University of Southampton iGEM 2009 <img src="http://www.personal.soton.ac.uk/jeh2v07/images/mainfoot.gif" width="975" height="61" />