Uppsala-Sweden/21 July 2009

Butanol survival experiment

Goal: Measure isobutanol tolerance of Synecocystis. Will be done using triplicates of isobutanol in E-flasks.

Volumes: 15 ml final for E-flask. Concentrations: 8 2, 1, 0,5, 0,25 , 0 vol% percent isobutanol.

Growth media: BG-11, nitrate supplemented as dictated by standard protocol.

11:00 OD measurement of start culture. OD found to be at 0,273.., within linear range as established 09-07-16 (OD 0,334-0,042)

See [[Media:Butanol+survival+test+conc090721.xls]]

OD Measurment 18:23 Correct amount of culture in each flask verified.

--Anders 11:40, 23 July 2009 (UTC)