File:Allignments fMT.jpg

Allignments of the metallothionein fMT constructs, above is In silico construct, below is sequence determined with VF2 primer. The first is without promotor ( BBa_K190019 ), second with constitutive promotor BBa_J23109 ( BBa_K190031 ) and the third with IPTG inducible promotor BBa_R0010 ( BBa_K190032 ). RBS is BBa_B0034. In combining the promotor and the coding sequence the Partregistry seems to add two unexpected additional bases (ag) between the two, missing in sequenced sample. In preparing the In silico construct a different prefix (lacking two bases (ag)), seen in sequenced samples, was used. The n's in the suffix of BBa_K190032 were determined to be a g as expected when giving a closer look into the chromatogram.