Team:PKU Beijing/Notebook/AND Gate 1/AraC SupD Assembly

Notebook > AND_Gate_1 > AraC SupD Assembly

2009.8.3
00:00 Ligation: Insert: pcr1, pla1 Vector: SupD, 1-12O

12:00 Transformation: yesterday’s ligation product

21:45 Grow the strains of pla1+SupD1, pcr1+1-12O1~4, pla1+1-12O1~4

22:10 Ligation: Insert: pcr1 Vector: SupD

2009.8.4
11:00 Transformation: pcr1+SupD

13:00 Mini-prep: pla+1-12O1~4, pcr+1-12O1~4, plas1

18:30 Double-enzyme digestion: pla+1-12O3, pcr+1-12O1, pla+SupD1

22:30 Double-enzyme digestion: pla+1-12O3, pcr+1-12O1

2009.8.5
11:00 Sent samples for sequencing

16:00 Ligation: Insert: pcr1, pla2 Vector: 1-12O

2009.8.6
15:00 Inducing test Strain: pla+1-12O1, pcr+1-12O1

15:30 Double-enzyme digestion Plasmid: pla+1-12O2, pcr+1-12O2

2009.8.7
15:00 Double-enzyme digestion: pcr1~6