Team:Kyoto/GSDD/Notebook/0810-0816



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To Do

 * Transformation
 * 1-N-1
 * 1-K-14
 * 2-N-13

colony

 * 1-N-1:observed
 * 1-K-14:observed
 * 2-N-13(1):none
 * 2-N-13(2):none
 * 2-N-13(3):none

We didn’t check that 2-N-13 was Kanamycin resistant at first(1,2). We made LB plate(+Kan), and tried the transformation again(3). However, no colony was observed. PCR amplification is required for amplifying 2-N-13.