IBB Pune/6 October 2009

We isolated the first set of ligations of


 * PR stands for Promoter-RBS BBa_J13002
 * Cprom stands for Constitutive promoter BBa_J23102

1) PLuxR-LuxI in pSB1Ak3 2) PR-GFP in pSB1Ak3 3) PLuxR-LuxI in pSB1A2 4) PR-GFP in pSB1A2

Assembly:

Digestion: 1) PR                     ES 2) Cprom                   ES 3) PluxR                   ES 4) LuxR                    SP 5) LacO                    SP 6) pSB1A2                  EP

Ligations:

1) pLuxR-lacO-in pSB1A2 2) PR-LuxR   in pSB1A2 3) Cprom-LuxR in pSB1A2