ULB/11 August 2009



 iGEM Team:ULB-Brussels Wiki  

  /* Design for http://2009.igem.org/Team:ULB-Brussels

Université Libre de Bruxelles

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 Home Team Project</a></li> Parts</a></li> Safety</a></li> Notebook</a></li> Sponsors</a></li> </ul> <ul>

</ul>

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{| align="right"

Start of construction (ligation) in Standard 10.

1st building: the promoter (BBa_R0011) and RBS (BBa_B0034)in a plasmid destination which contain the brig CcdB (BBa_P1010) (it induces cell death) and a resistance gene. As our plasmids are resistant to ampicillin, the plasmid is chosen destination pSB1C3 with brick BBa_P1010( resistance to chloramphenicol).

Laboratory manipulation

 * Thermoporation of BBa_I51020 (plasmid destination resistant to ampicillin) with thermo-skill bacteria thermocompétentes.
 * Digestion of BBa_I51020 with EcoRI and PstI.

Computer part
Creating BioBricks (CCDA in the forward and reverse direction).
 * Order of primers for amplification ccdA + its weak constitutive promoter (promoter mob, not from E. Coli)
 * Creating BioBricks (CCDA in the forward and reverse direction)
 * http://partsregistry.org/Part:BBa_K196000:Design
 * http://partsregistry.org/Part:BBa_K196001:Design