HKU-HKBU/29 July 2009

Co-transformation Transform two plasmids (A+, C+) to MG3.① Plac-CheZ-His-TT  C+② Lac Iq   A+

15min.Centrifuge in 4000rmp at 0℃ for 2min.Decent supernatant
Add iced DI water, re-suspend Centrifuge in 8000rmp for 2min. Pipette away supernatant. (Above three steps should be repeated for 3 times.) Remove most supernatant, leaving behind~50μL. Electro-transformation

18:40 take the bacteria out from warm room
Transfer to agar plates with A+ & C+.