Team:Berkeley Wetlab/Recipes

10X TBST
31.4 g TRIS HCl 80g NaCl 10ml Tween-20 bring volume to 1000ml pH 7.5

1X TBS
605 mg Tris 876 mg NaCl 80 ml H2O pH 7.4 bring volume to 100 ml

Artificial Seawater
500 ml ddH2O 12.2 g NaCl 2.6 g MgCl2 .57 g CaCl2

Tris Buffer + 2% SDS
50 mM Tris 150 mM NaCl 2% SDS pH 7

Precipitation Buffer
2 mL of acetic acid Add concentrated NaOH until the pH is 5 Add water to reach 10 mL total volume volume 0.2 g ZnCl Add 40 mL ethanol

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