Template:Team:KULeuven/31 August 2009/VanillinReceptor


 * TOPO cloning showed only blue colonies so we searched for alternatives. We did resaerchwork if it was maybe possible with a puc-vector
 * The mutation of rpoA was again checked but gelelectrophoresis showed no result
 * X,Y and K (the igemvector) were cut with EcoR1 and Pst1.
 * new ligation was done with the first cuttingproduct so today it was electroporated and plated.
 * PCR was done for W to get more basicproduct to work with