Team:Brown/Notebook Recipes/SMMP

Making SMMP Medium ( Recovery Medium for Electroporated Staphylococcus epidermidis cells)

•	Making 100ml of SMMP 1.	55.5 parts SMM Buffer a.	Sucrose 			18.8263g b.	Maleic Acid			0.2554g c.	MgCl2      6 H2O 		0.4473g d.	dd H2O			55ml

2.	4 parts 7% Panassy Broth a.	100% Panassy Broth 		2.8ml b.	ddH2O				40ml

3.	0.5 parts 10% BSA a.	ddH2O				4.95ml ➢	Autoclave the solution. 10x BSA			50ul