August/13 October 2009

1.colony check sample    no. of colony 67            ++   68             ++   71             +   73             +   79             +    80             +   81             +   82             +   83             +   84             +

2.digation K204072

K204090

37degree, 2hr gel cut& purification ligation

3.min prep sample   conc. 68          53 ng/uL 142                 76                  59

4.digation

K204091

K204092

K204093

K204094

K204095

K204096

↓ 37 degree, 2hr gel cut & purification ligation

5.min prep sample        conc. 67                87  ng/uL 68                28  71                 64  73                 12  79                   5  80                   9  81                 75  82                 14  83                 19  84                 15

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