Illinois/25 June 2009

June 25
Modeling

We created a working program in SimBiology that roughly models our decoder. In the program, each gene is regulated by two sRNAs. The sRNA synthesis rates are assumed to be constant for now, whereas in reality these rates will depend on input concentrations and promoter activity. We verified by changing sRNA synthesis rates that combinations of two sRNAs resulted in production of the correct fluorescent protein.



We cultured cells overnight in anticipation that we will be able to perform a fluorescence reading tomorrow.

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