Team:Groningen/Notebook/1 August 2009

Miscellaneous
ON Cultures: KH1: No growth KL1: No growth KL2: No growth AL1: No growth AH2: Minimal growth Left the ones with no growth on the bench, de rest was spinned down and pellets frozen. Pellets of AL2, KM1, KH2 & AH1 were red of colour.

Plates: Glpf in pSB1AC (31/07/09, TY amp120) & Glpf con. pSB1AC (31/07/09, TY amp120) both Empty, left them on the bench. A lot of other plates in incubator I was not told of, left them in the incubator (No growth)

Vectors
Transformation + controls are completely full. Zn-RBS (50 μL) is covered in growth, no single colonies comparable to positive controls (200 μL). Other plates seem to have no colonies, at least not identifiable next to agar debris on plates (bad quality plates). If these were to be identified as colonies they are also present on negative controls (MilliQ & Selfcloser). Plates are left on the bench, Zn-RBS is put to 4 °C