Wisconsin-Madison/1 July 2009

July 1, 2009
Other: Primer Design (MevT, MBI, MBIS, MetK, gsSDMT, pSBA1, replacement promoter)

Find promoter from Registry to replace MevT promoter

Make Plasmid Maps (everything but MBIS, MevT)

Research

Look into DXP pathway

More Efficient Enzymes from Mevalanate Pathway

Attended GLBRC Seminar

Ex 17: Salt Induced ProU GFP tests

Test 5: FAIL - CONTAMINATION

Ex 15: Inducing Various Modified (triple) E.Coli Strains

- Plate liquid culture of isopentenal before we add Ethyl Acetate. Syd said cells are supposed to die during induction

1. Control 33+99: Cm+Amp

2. MG1655 W: Trip

3. MG1655 Delta Ara: Trip

4. BL21 (DE3): Trip

Ran out of Triple Plates, Could only plate one sample (MG1655 Wildtype) Growth - Av

- Add layer of Ethyl Acetate – 0.5 mM

- GC Time

Ex 10: Cyanobacteria

Added DI water to cyanobacteria cultures which lose water due to aeration (DI wasnt sterile, in future use sterile DI)