Team:Freiburg bioware/Notebook/His+FluA+Split+FokI pMA

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Commented GenBank file <li> <a href="http://2009.igem.org/wiki/images/d/d8/Freiburg09_His%2BFluA%2BSplit%2BFokI_pMA.txt">His+FluA+Split+Foki_pMA.txt</a></li>

His+FluA+SplitLinker+Foki in pMA Vector

<meta name="GENERATOR" content="Clone Manager"> Clone Manager Data <body bgcolor = "#FFFFFF" text = "#000000"> <font face="Courier New"> 1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caccgtgaaa agccccttta cacgcgcctt ggggataaac aaataaaaag atttatgtaa <font color="#009900"> r  w  h  f   s  g  k   c  a  r   n  p  y  l   f  i  f   l  n  t

61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa gtttatacat aggcgagtac tctgttattg ggactattta cgaagttatt ataacttttt <font color="#009900"> f  k  y  v   s  a  h   e  t  i   t  l  i  n   a  s  i   i  l  k

121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt ccttctcata ctcataagtt gtaaaggcac agcgggaata agggaaaaaa cgccgtaaaa <font color="#009900"> k  e  e  y   e  y  s   t  f  p   c  r  p  y   s  l  f   c  g  i <font color="#009900">               &gt;&gt;........................AmpR..........................&gt; <font color="#009900">            m  s  i  q   h  f  r   v  a  l   i  p  f  f   a  a  f

181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt cggaaggaca aaaacgagtg ggtctttgcg accactttca ttttctacga cttctagtca <font color="#009900"> l  p  s  c   f  c  s   p  r  n   a  g  e  s   k  r  c   -  r  s

<font color="#009900">      &gt;.............................AmpR..............................&gt; <font color="#009900">  c  l  p   v  f  a  h   p  e  t   l  v  k   v  k  d  a   e  d  q

241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt acccacgtgc tcacccaatg tagcttgacc tagagttgtc gccattctag gaactctcaa <font color="#009900"> v  g  c  t   s  g  l   h  r  t   g  s  q  q   r  -  d   p  -  e <font color="#009900">       &gt;.............................AmpR..............................&gt; <font color="#009900">  l  g  a   r  v  g  y   i  e  l   d  l  n   s  g  k  i   l  e  s

301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg aagcggggct tcttgcaaaa ggttactact cgtgaaaatt tcaagacgat acaccgcgcc <font color="#009900"> f  s  p  r   r  t  f   s  n  d   e  h  f  -   s  s  a   m  w  r <font color="#009900">       &gt;.............................AmpR..............................&gt; <font color="#009900">  f  r  p   e  e  r  f   p  m  m   s  t  f   k  v  l  l   c  g  a

361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga ataatagggc ataactgcgg cccgttctcg ttgagccagc ggcgtatgtg ataagagtct <font color="#009900"> g  i  i  p   y  -  r   r  a  r   a  t  r  s   p  h  t   l  f  s

<font color="#009900">      &gt;.............................AmpR..............................&gt; <font color="#009900">  v  l  s   r  i  d  a   g  q  e   q  l  g   r  r  i  h   y  s  q

421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa tactgaacca actcatgagt ggtcagtgtc ttttcgtaga atgcctaccg tactgtcatt <font color="#009900"> e  -  l  g   -  v  l   t  s  h   r  k  a  s   y  g  w   h  d  s <font color="#009900">       &gt;.............................AmpR..............................&gt; <font color="#009900">  n  d  l   v  e  y  s   p  v  t   e  k  h   l  t  d  g   m  t  v

481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga ctcttaatac gtcacgacgg tattggtact cactattgtg acgccggttg aatgaagact <font color="#009900"> k  r  i  m   q  c  c   h  n  h   e  -  -  h   c  g  q   l  t  s <font color="#009900">       &gt;.............................AmpR..............................&gt; <font color="#009900">  r  e  l   c  s  a  a   i  t  m   s  d  n   t  a  a  n   l  l  l

541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa gttgctagcc tcctggcttc ctcgattggc gaaaaaacgt gttgtacccc ctagtacatt <font color="#009900"> d  n  d  r   r  t  e   g  a  n   r  f  f  a   q  h  g   g  s  c

<font color="#009900">      &gt;.............................AmpR..............................&gt; <font color="#009900">  t  t  i   g  g  p  k   e  l  t   a  f  l   h  n  m  g   d  h  v

601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca gagcggaact agcaaccctt ggcctcgact tacttcggta tggtttgctg ctcgcactgt <font color="#009900"> n  s  p  -   s  l  g   t  g  a   e  -  s  h   t  k  r   r  a  - <font color="#009900">      &gt;.............................AmpR..............................&gt; <font color="#009900">  t  r  l   d  r  w  e   p  e  l   n  e  a   i  p  n  d   e  r  d

661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ggtgctacgg acatcgttac cgttgttgca acgcgtttga taattgaccg cttgatgaat <font color="#009900"> h  h  d  a   c  s  n   g  n  n   v  a  q  t   i  n  w   r  t  t <font color="#009900">       &gt;.............................AmpR..............................&gt; <font color="#009900">  t  t  m   p  v  a  m   a  t  t   l  r  k   l  l  t  g   e  l  l

721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac gagatcgaag ggccgttgtt aattatctga cctacctccg cctatttcaa cgtcctggtg <font color="#009900"> y  s  s  f   p  a  t   i  n  r   l  d  g  g   g  -  s   c  r  t

<font color="#009900">      &gt;.............................AmpR..............................&gt; <font color="#009900">  t  l  a   s  r  q  q   l  i  d   w  m  e   a  d  k  v   a  g  p

781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc aagacgcgag ccgggaaggc cgaccgacca aataacgact atttagacct cggccactcg <font color="#009900"> t  s  a  l   g  p  s   g  w  l   v  y  c  -   -  i  w   s  r  - <font color="#009900">      &gt;.............................AmpR..............................&gt; <font color="#009900">  l  l  r   s  a  l  p   a  g  w   f  i  a   d  k  s  g   a  g  e

841 gtggttctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag caccaagagc gccatagtaa cgtcgtgacc ccggtctacc attcgggagg gcatagcatc <font color="#009900"> a  w  f  s   r  y  h   c  s  t   g  a  r  w   -  a  l   p  y  r <font color="#009900">       &gt;.............................AmpR..............................&gt; <font color="#009900">  r  g  s   r  g  i  i   a  a  l   g  p  d   g  k  p  s   r  i  v

901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga aatagatgtg ctgcccctca gtccgttgat acctacttgc tttatctgtc tagcgactct <font color="#009900"> s  y  l  h   d  g  e   s  g  n   y  g  -  t   k  -  t   d  r  -

<font color="#009900">      &gt;.............................AmpR..............................&gt; <font color="#009900">  v  i  y   t  t  g  s   q  a  t   m  d  e   r  n  r  q   i  a  e

961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt atccacggag tgactaattc gtaaccattg acagtctggt tcaaatgagt atatatgaaa <font color="#009900"> d  r  c  l   t  d  -   a  l  v   t  v  r  p   s  l  l   i  y  t <font color="#009900">       &gt;............AmpR............&gt;&gt; <font color="#009900">  i  g  a   s  l  i  k   h  w  -

1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata tctaactaaa ttttgaagta aaaattaaat tttcctagat ccacttctag gaaaaactat <font color="#009900"> l  d  -  f   k  t  s   f  l  i   -  k  d  l   g  e  d   p  f  -

1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag tagagtactg gttttaggga attgcactca aaagcaaggt gactcgcagt ctggggcatc <font color="#009900"> -  s  h  d   q  n  p   l  t  -   v  f  v  p   l  s  v   r  p  r

1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa ttttctagtt tcctagaaga actctaggaa aaaaagacgc gcattagacg acgaacgttt <font color="#009900"> r  k  d  q   r  i  f   l  r  s   f  f  s  a   r  n  l   l  l  a

1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt gtttttttgg tggcgatggt cgccaccaaa caaacggcct agttctcgat ggttgagaaa <font color="#009900"> n  k  k  t   t  a  t   s  g  g   l  f  a  g   s  r  a   t  n  s

1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc aaggcttcca ttgaccgaag tcgtctcgcg tctatggttt atgacaagaa gatcacatcg <font color="#009900"> f  s  e  g   n  w  l   q  q  s   a  d  t  k   y  c  s   s  s  v

1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa gcatcaatcc ggtggtgaag ttcttgagac atcgtggcgg atgtatggag cgagacgatt <font color="#009900"> a  v  v  r   p  p  l   q  e  l   c  s  t  a   y  i  p   r  s  a

1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa aggacaatgg tcaccgacga cggtcaccgc tattcagcac agaatggccc aacctgagtt <font color="#009900"> n  p  v  t   s  g  c   c  q  w   r  -  v  v   s  y  r   v  g  l

1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ctgctatcaa tggcctattc cgcgtcgcca gcccgacttg ccccccaagc acgtgtgtcg <font color="#009900"> k  t  i  v   t  g  -   g  a  a   v  g  l  n   g  g  f   v  h  t

1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa ggtcgaacct cgcttgctgg atgtggcttg actctatgga tgtcgcactc gatactcttt <font color="#009900"> a  q  l  g   a  n  d   l  h  r   t  e  i  p   t  a  -   a  m  r

1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa cgcggtgcga agggcttccc tctttccgcc tgtccatagg ccattcgccg tcccagcctt <font color="#009900"> k  r  h  a   s  r  r   e  k  g   g  q  v  s   g  k  r   q  g  r

1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg gtcctctcgc gtgctccctc gaaggtcccc ctttgcggac catagaaata tcaggacagc <font color="#009900"> n  r  r  a   h  e  g   a  s  r   g  k  r  l   v  s  l   -  s  c

1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc ccaaagcggt ggagactgaa ctcgcagcta aaaacactac gagcagtccc cccgcctcgg <font color="#009900"> r  v  s  p   p  l  t   -  a  s   i  f  v  m   l  v  r   g  a  e

1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ataccttttt gcggtcgttg cgccggaaaa atgccaagga ccggaaaacg accggaaaac <font color="#009900"> p  m  e  k   r  q  q   r  g  l   f  t  v  p   g  l  l   l  a  f

1801 ctcattaggc accccaggct ttacccgaac gaccgagcgc agcgagtcag tgagcgagga gagtaatccg tggggtccga aatgggcttg ctggctcgcg tcgctcagtc actcgctcct <font color="#009900"> c  s  l  g   t  p  g   f  t  r   t  t  e  r   s  e  s   v  s  e

1861 agcggagagc gcccaatacg caaggaaaca gctatgacca tgttaatgca gctggcacga tcgcctctcg cgggttatgc gttcctttgt cgatactggt acaattacgt cgaccgtgct <font color="#009900"> e  a  e  s   a  q  y   a  r  k   q  l  -  p   c  -  c   s  w  h

1921 caggtttccc gactggaaag cgggcagtga gcggaaggcc catgaggcca gttaattaag gtccaaaggg ctgacctttc gcccgtcact cgccttccgg gtactccggt caattaattc <font color="#009900"> d  r  f  p   d  w  k   a  g  s   e  r  k  a   h  e  a   s  -  l

<font color="#0000FF">NotI <font color="#0000FF">--+-- <font color="#0000FF">EcoRI           <font color="#0000FF">XbaI    <font color="#0000FF">NgoMIV

<font color="#0000FF">-+-         <font color="#0000FF">-+   <font color="#0000FF">-+ 1981 aggtaccgaa ttcgcggccg cttctagatg gccggccatc atcatcatca tcataccggc tccatggctt aagcgccggc gaagatctac cggccggtag tagtagtagt agtatggccg <font color="#009900"> r  g  t  e   f  a  a   a  s  r   w  p  a  i   i  i  i   i  i  p <font color="#009900">                                    &gt;&gt;.............ORF-1...............&gt; <font color="#009900">                                m   a  g  h   h  h  h   h  h  t  g

<font color="#009900">                                             &gt;&gt;....His Tag.....&gt;&gt; <font color="#009900">                                          h   h  h  h   h  h

2041 gacgtgtacc acgacggcgc ctgccccgaa gtgaagcccg tggacaactt cgactggtcc ctgcacatgg tgctgccgcg gacggggctt cacttcgggc acctgttgaa gctgaccagg <font color="#009900"> a  t  c  t   t  t  a   p  a  p   k  -  s  p   w  t  t   s  t  g <font color="#009900">       &gt;.............................ORF-1.............................&gt; <font color="#009900">   d  v  y   h  d  g   a  c  p  e   v  k  p   v  d  n   f  d  w  s

<font color="#009900">      &gt;&gt;............................FluA..............................&gt; <font color="#009900">   d  v  y   h  d  g   a  c  p  e   v  k  p   v  d  n   f  d  w  s

2101 cagtaccacg gcaagtggtg ggaggtggcc aagtacccca gccccaacgg caagtatggc gtcatggtgc cgttcaccac cctccaccgg ttcatggggt cggggttgcc gttcataccg <font color="#009900"> p  s  t  t   a  s  g   g  r  w   p  s  t  p   a  p  t   a  s  m <font color="#009900">       &gt;.............................ORF-1.............................&gt; <font color="#009900">   q  y  h   g  k  w   w  e  v  a   k  y  p   s  p  n   g  k  y  g

<font color="#009900">      &gt;.............................FluA..............................&gt; <font color="#009900">   q  y  h   g  k  w   w  e  v  a   k  y  p   s  p  n   g  k  y  g

2161 aagtgcggct gggccgagta cacccccgag ggcaagagcg tgaaggtgtc cagatacgac ttcacgccga cccggctcat gtgggggctc ccgttctcgc acttccacag gtctatgctg <font color="#009900"> a  s  a  a   g  p  s   t  p  p   r  a  r  a   -  r  c   p  d  t <font color="#009900">       &gt;.............................ORF-1.............................&gt; <font color="#009900">   k  c  g   w  a  e   y  t  p  e   g  k  s   v  k  v   s  r  y  d

<font color="#009900">      &gt;.............................FluA..............................&gt; <font color="#009900">   k  c  g   w  a  e   y  t  p  e   g  k  s   v  k  v   s  r  y  d

2221 gtgatccacg gcaaagaata cttcatggaa ggcaccgcct accccgtggg cgacagcaag cactaggtgc cgtttcttat gaagtacctt ccgtggcgga tggggcaccc gctgtcgttc <font color="#009900"> t  -  s  t   a  k  n   t  s  w   k  a  p  p   t  p  w   a  t  a <font color="#009900">       &gt;.............................ORF-1.............................&gt; <font color="#009900">   v  i  h   g  k  e   y  f  m  e   g  t  a   y  p  v   g  d  s  k

<font color="#009900">      &gt;.............................FluA..............................&gt; <font color="#009900">   v  i  h   g  k  e   y  f  m  e   g  t  a   y  p  v   g  d  s  k

2281 atcggcaaga tctaccacag ccggaccgtg ggcggctaca ccagaaagac cgtgttcaac tagccgttct agatggtgtc ggcctggcac ccgccgatgt ggtctttctg gcacaagttg <font color="#009900"> r  s  a  r   s  t  t   a  g  p   w  a  a  t   p  e  r   p  c  s <font color="#009900">       &gt;.............................ORF-1.............................&gt; <font color="#009900">   i  g  k   i  y  h   s  r  t  v   g  g  y   t  r  k   t  v  f  n

<font color="#009900">      &gt;.............................FluA..............................&gt; <font color="#009900">   i  g  k   i  y  h   s  r  t  v   g  g  y   t  r  k   t  v  f  n

2341 gtgctgtcca ccgacaacaa gaactacatc atcggctaca gctgccgcta cgacgaggac cacgacaggt ggctgttgtt cttgatgtag tagccgatgt cgacggcgat gctgctcctg <font color="#009900"> t  c  c  p   p  t  t   r  t  t   s  s  a  t   a  a  a   t  t  r <font color="#009900">       &gt;.............................ORF-1.............................&gt; <font color="#009900">   v  l  s   t  d  n   k  n  y  i   i  g  y   s  c  r   y  d  e  d

<font color="#009900">      &gt;.............................FluA..............................&gt; <font color="#009900">   v  l  s   t  d  n   k  n  y  i   i  g  y   s  c  r   y  d  e  d

2401 aagaagggcc actgggacca cgtgtgggtg ctgtcccggt ccatggtgct gaccggcgag ttcttcccgg tgaccctggt gcacacccac gacagggcca ggtaccacga ctggccgctc <font color="#009900"> t  r  r  a   t  g  t   t  c  g   c  c  p  g   p  w  c   -  p  a <font color="#009900">       &gt;.............................ORF-1.............................&gt; <font color="#009900">   k  k  g   h  w  d   h  v  w  v   l  s  r   s  m  v   l  t  g  e

<font color="#009900">      &gt;.............................FluA..............................&gt; <font color="#009900">   k  k  g   h  w  d   h  v  w  v   l  s  r   s  m  v   l  t  g  e

2461 gccaagaccg ccgtggagaa ctacctgatc ggcagccccg tggtggacag ccagaaactg cggttctggc ggcacctctt gatggactag ccgtcggggc accacctgtc ggtctttgac <font color="#009900"> r  p  r  p   p  w  r   t  t  -   s  a  a  p   w  w  t   a  r  n <font color="#009900">       &gt;.............................ORF-1.............................&gt; <font color="#009900">   a  k  t   a  v  e   n  y  l  i   g  s  p   v  v  d   s  q  k  l

<font color="#009900">      &gt;.............................FluA..............................&gt; <font color="#009900">   a  k  t   a  v  e   n  y  l  i   g  s  p   v  v  d   s  q  k  l

2521 gtgtacagcg acttcagcga ggccgcctgc aaagtgaaca acaccggccg accagcctgt cacatgtcgc tgaagtcgct ccggcggacg tttcacttgt tgtggccggc tggtcggaca <font color="#009900"> w  c  t  a   t  s  a   r  p  p   a  k  -  t   t  p  a   d  q  p <font color="#009900">       &gt;.............................ORF-1.............................&gt; <font color="#009900">   v  y  s   d  f  s   e  a  a  c   k  v  n   n  t  g   r  p  a  c

<font color="#009900">      &gt;....................FluA...................&gt;&gt; <font color="#009900">   v  y  s   d  f  s   e  a  a  c   k  v  n   n <font color="#009900">                                              Split-Linker &gt;&gt;..........&gt; <font color="#009900">                                                        r  p  a  c

2581 aagattccaa atgacctgaa gcagaaagtt atgaatcaca ccggcaaatc tgaactggag ttctaaggtt tactggactt cgtctttcaa tacttagtgt ggccgtttag acttgacctc <font color="#009900"> v  r  f  q   m  t  -   s  r  k   l  -  i  t   p  a  n   l  n  w

<font color="#009900">      &gt;.............................ORF-1.............................&gt; <font color="#009900">   k  i  p   n  d  l   k  q  k  v   m  n  h   t  g  k   s  e  l  e <font color="#009900">       &gt;..............Split-Linker.............&gt;&gt; <font color="#009900">   k  i  p   n  d  l   k  q  k  v   m  n  h <font color="#009900">                                                     &gt;&gt;.'Fok inaktiv'..&gt;

<font color="#009900">                                                 g  k   s  e  l  e

2641 gagaaaaaat ccgagctgcg ccacaaactg aaatatgtgc ctcacgagta tatcgaactg ctctttttta ggctcgacgc ggtgtttgac tttatacacg gagtgctcat atagcttgac <font color="#009900"> r  r  k  n   p  s  c   a  t  n   -  n  m  c   l  t  s   i  s  n <font color="#009900">       &gt;.............................ORF-1.............................&gt; <font color="#009900">   e  k  k   s  e  l   r  h  k  l   k  y  v   p  h  e   y  i  e  l

<font color="#009900">      &gt;.........................'Fok inaktiv'.........................&gt; <font color="#009900">   e  k  k   s  e  l   r  h  k  l   k  y  v   p  h  e   y  i  e  l

2701 atcgagatcg cccgtaatag tacccaagac cgtatcctgg aaatgaaagt gatggagttc tagctctagc gggcattatc atgggttctg gcataggacc tttactttca ctacctcaag <font color="#009900"> -  s  r  s   p  v  i   v  p  k   t  v  s  w   k  -  k   -  w  s <font color="#009900">       &gt;.............................ORF-1.............................&gt; <font color="#009900">   i  e  i   a  r  n   s  t  q  d   r  i  l   e  m  k   v  m  e  f

<font color="#009900">      &gt;.........................'Fok inaktiv'.........................&gt; <font color="#009900">   i  e  i   a  r  n   s  t  q  d   r  i  l   e  m  k   v  m  e  f

2761 ttcatgaaag tctatggcta tcgtggcaaa catctgggtg gtagccgtaa accagcaggt aagtactttc agataccgat agcaccgttt gtagacccac catcggcatt tggtcgtcca <font color="#009900"> s  s  -  k   s  m  a   i  v  a   n  i  w  v   v  a  v   n  q  q <font color="#009900">       &gt;.............................ORF-1.............................&gt; <font color="#009900">   f  m  k   v  y  g   y  r  g  k   h  l  g   g  s  r   k  p  a  g

<font color="#009900">      &gt;.........................'Fok inaktiv'.........................&gt; <font color="#009900">   f  m  k   v  y  g   y  r  g  k   h  l  g   g  s  r   k  p  a  g

2821 gccatttata ccgttggttc cccgatcgat tatggcgtta tcgtggccac aaaagcgtat cggtaaatat ggcaaccaag gggctagcta ataccgcaat agcaccggtg ttttcgcata <font color="#009900"> v  p  f  i   p  l  v   p  r  s   i  m  a  l   s  w  p   q  k  r <font color="#009900">       &gt;.............................ORF-1.............................&gt; <font color="#009900">   a  i  y   t  v  g   s  p  i  d   y  g  v   i  v  a   t  k  a  y

<font color="#009900">      &gt;.........................'Fok inaktiv'.........................&gt; <font color="#009900">   a  i  y   t  v  g   s  p  i  d   y  g  v   i  v  a   t  k  a  y

2881 tctggcggtt ataatctgcc gattggtcag gctgatgaga tggaacgtta tgtggaagag agaccgccaa tattagacgg ctaaccagtc cgactactct accttgcaat acaccttctc <font color="#009900"> i  l  a  v   i  i  c   r  l  v   r  l  m  r   w  n  v   m  w  k <font color="#009900">       &gt;.............................ORF-1.............................&gt; <font color="#009900">   s  g  g   y  n  l   p  i  g  q   a  d  e   m  e  r   y  v  e  e

<font color="#009900">      &gt;.........................'Fok inaktiv'.........................&gt; <font color="#009900">   s  g  g   y  n  l   p  i  g  q   a  d  e   m  e  r   y  v  e  e

2941 aatcagaccc gtaacaaaca tctgaacccg aacgaatggt ggaaagtgta tccgtcaagt ttagtctggg cattgtttgt agacttgggc ttgcttacca cctttcacat aggcagttca <font color="#009900"> r  i  r  p   v  t  n   i  -  t   r  t  n  g   g  k  c   i  r  q <font color="#009900">       &gt;.............................ORF-1.............................&gt; <font color="#009900">   n  q  t   r  n  k   h  l  n  p   n  e  w   w  k  v   y  p  s  s

<font color="#009900">      &gt;.........................'Fok inaktiv'.........................&gt; <font color="#009900">   n  q  t   r  n  k   h  l  n  p   n  e  w   w  k  v   y  p  s  s

3001 gtcaccgagt tcaaatttct gttcgtgagc ggccacttta aaggcaacta taaagcccag cagtggctca agtttaaaga caagcactcg ccggtgaaat ttccgttgat atttcgggtc <font color="#009900"> v  s  p  s   s  n  f   c  s  -   a  a  t  l   k  a  t   i  k  p <font color="#009900">       &gt;.............................ORF-1.............................&gt; <font color="#009900">   v  t  e   f  k  f   l  f  v  s   g  h  f   k  g  n   y  k  a  q

<font color="#009900">      &gt;.........................'Fok inaktiv'.........................&gt; <font color="#009900">   v  t  e   f  k  f   l  f  v  s   g  h  f   k  g  n   y  k  a  q

3061 ctgactcgtc tgaaccatat caccaatagc aatggggcag tgctgagtgt tgaggaactg gactgagcag acttggtata gtggttatcg ttaccccgtc acgactcaca actccttgac <font color="#009900"> s  -  l  v   -  t  i   s  p  i   a  m  g  q   c  -  v   l  r  n <font color="#009900">       &gt;.............................ORF-1.............................&gt; <font color="#009900">   l  t  r   l  n  h   i  t  n  s   n  g  a   v  l  s   v  e  e  l

<font color="#009900">      &gt;.........................'Fok inaktiv'.........................&gt; <font color="#009900">   l  t  r   l  n  h   i  t  n  s   n  g  a   v  l  s   v  e  e  l

3121 ctgatcggtg gagaaatgat caaagcaggc accctgactc tggaagaagt tcgccgtaaa gactagccac ctctttacta gtttcgtccg tgggactgag accttcttca agcggcattt <font color="#009900"> c  -  s  v   e  k  -   s  k  q   a  p  -  l   w  k  k   f  a  v <font color="#009900">       &gt;.............................ORF-1.............................&gt; <font color="#009900">   l  i  g   g  e  m   i  k  a  g   t  l  t   l  e  e   v  r  r  k

<font color="#009900">      &gt;.........................'Fok inaktiv'.........................&gt; <font color="#009900">   l  i  g   g  e  m   i  k  a  g   t  l  t   l  e  e   v  r  r  k

<font color="#0000FF">PstI <font color="#0000FF">--+ <font color="#0000FF">SpeI    <font color="#0000FF">NotI

<font color="#0000FF">-+ <font color="#0000FF">--+- 3181 ttcaacaatg gcgagatcaa ttttaccggt taatactagt agcggccgct gcagagctca aagttgttac cgctctagtt aaaatggcca attatgatca tcgccggcga cgtctcgagt <font color="#009900"> n  s  t  m   a  r  s   i  l  p   v  n  t  s   s  g  r   c  r  a <font color="#009900">       &gt;..............ORF-1..............&gt;&gt; <font color="#009900">   f  n  n   g  e  i   n  f  t  g   -

<font color="#009900">      &gt;..........'Fok inaktiv'.........&gt;&gt; <font color="#009900">   f  n  n   g  e  i   n  f  t  g

3241 tggcgcgcct aggccttgac ggccttccgc caattcgccc tatagtgagt cgtattacgt accgcgcgga tccggaactg ccggaaggcg gttaagcggg atatcactca gcataatgca <font color="#009900"> h  g  a  p   r  p  -   r  p  s   a  n  s  p   y  s  e   s  y  y

3301 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac gcgcgagtga ccggcagcaa aatgttgcag cactgaccct tttgggaccg caatgggttg <font color="#009900"> v  a  l  t   g  r  r   f  t  t   s  -  l  g   k  p  w   r  y  p

3361 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca aattagcgga acgtcgtgta gggggaaagc ggtcgaccgc attatcgctt ctccgggcgt <font color="#009900"> t  -  s  p   c  s  t   s  p  f   r  q  l  a   -  -  r   r  g  p

3421 ccgaaacgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggagcg ccctgtagcg ggctttgcgg gaagggttgt caacgcgtcg gacttaccgc ttaccctcgc gggacatcgc <font color="#009900"> h  r  n  a   l  p  n   s  c  a   a  -  m  a   n  g  s   a  l  -

3481 gccactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc cggtgagttg ggatagagcc agataagaaa actaaatatt ccctaaaacg gctaaagccg <font color="#009900"> r  p  l  n   p  i  s   v  y  s   f  d  l  -   g  i  l   p  i  s

3541 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt gataaccaat tttttactcg actaaattgt ttttaaattg cgcttaaaat tgttttataa <font color="#009900"> a  y  w  l   k  n  e   l  i  -   q  k  f  n   a  n  f   n  k  i

3601 aacgcttaca atttag ttgcgaatgt taaatc <font color="#009900"> l  t  l  t   i  -

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