Wisconsin-Madison/4 August 2009

August 4, 2009
Cyanos: Transformations à 30°C room with Parafilm 1.     Low 1 – Culture started on 7/27/09 - 23 mg/ml 2.     Low 2 - Culture started on 7/27/09 – 32 mg/ml 3.     High 1 - Culture started on 7/20/09 – 23 mg/ml 4.     High 2 - Culture started on 7/20/09 – 23 mg/ml C: Plating: 1% BG-11 – no resistance: 40mL C: Plating: 1% BG-11 – Cm: 40mL Plating: 1% BG-11 – no resistance -20hrà  Cm C: Liquid: BG-11 – no resistance, 50:150 C: Liquid: BG-11 – Cm: 50:150 Liquid: BG-11 – no resistance 50:150 -20hrà  Cm
 * Liquid cultures of High 1 and Low 1 were placed in 30°C Shaker, minimal indirect light
 * Plated Cultures of High 1 and Low 1 were placed in minimal indirect light
 * Inoculated mevT, pBAD24, PSB1A3
 * prep of more PSB1A3
 * gel extraction of pBAD24, PSB1A3, GsSDMT, and PCR GsSDMT
 * double digest of the above with appropriate enzymes
 * ligation of the biobrick attempt
 * cyano transformation attempt