Team:Warsaw/Calendar-Main/2 October 2009

cloning of cro (another shot) Kuba

Task: Obtain a brick vector carrying the cro gene, with a full, correct prefix and suffic

Method used:
 * digest of pSB and cro PCR product with XbaI and SpeI
 * ligation
 * transformation
 * isolation
 * control digest with XbaI (in the inverse orientation, the insert eliminates this restriction site)

time elapsed: 3 days

results: the brick was succesfully obtained