Team:Groningen/Parts/Used Parts

Used Parts

 * To get to the partsregistry site of a particular part one can click on the name of the part
 * To see what our main findings where (no details or derivations) regarding a particular part one can click on more information

Miscellaneous
 BBa_B0034 RBS ((Elowitz 1999) -- defines RBS efficiency)

 BBa_B0014 Double terminator (B0012-B0011)

 BBa_I750016 GVP Gas Vesicle Proteins

 BBa_P1010 ccdB cell death gene

Promotors
 J23100 Constitutive promoter family member (high expression)

 J23101 Constitutive promoter family member (high expression, reference)

 J23106 Constitutive promoter family member (medium expression)

 J23109 Constitutive promoter family member (low expression)

 R0010 Promoter (lacI regulated) This part is an inverting regulator sensitive to LacI and CAP. It contains two protein binding sites. The first binds the CAP protein, which is generally present in E.coli and is asocciated with cell health and availability of glucose. The second binds LacI protein.

 I0500 pBad/araC

Vectors
 pSB1AC3 High copy BioBrick assembly plasmid

 pSB1A2 pSB1A2 (Replaced by pSB1A3 in registry)  pSB1A2 is a high copy number plasmid carrying ampicillin resistance. Together with pSB1AC3 the vector was used for most assemblies of our team, because the high copy number made it easy to work with and easy isolation.

 pSB2K3 A low copy number base vector A low copy number base vector which as a resistance against Kanamycin

 pSB3K3 A low copy number base vector A low copy number base vector which as a resistance against Kanamycin

 BBa_J61002 A normal base vector A normal base vector which has a resistance against Ampicillin

 BBa_J61035 Vector we get with GVP It's the backbone of our GVP part is and it a resistance against Ampicillin/Gentamycin