Team:Paris/Production testing

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WetLab - Production
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a.menu_sub_active { padding-left: 7px; padding-right: 7px; color:#b0310e; font-weight:bold; }  Main |  Addressing|  Production|  Reception


 * 1) The delay
 * 2) Part I : Testing delay phase I
 * 3) Part I : Functional testing of the part I
 * 4) Part II : Testing delay phase II
 * 5) Part II : Functional testing of the part II
 * 6) The destabilization of the membrane

 

The delay
We separate the construction on two plasmids : Part I and Part II.

Part I : Testing delay phase I
The green color means the experiment was a success



Experiments ran :

Part I : Functional testing of the part I
PBAD LacI LVA- YFP was transforme into Top ten Bacteria and grew on medium :

1% arabinose

1% glucose

LB pure

To learn more, see the Conclusion page

<img style="width:40px; height:40px;" src="http://2009.igem.org/wiki/images/1/10/Paris_Up.png"/></a>

<div id="paris_content_boxtop"> <div id="paris_content">

Part II : Testing delay phase II
<font style="color:green;font-weight:bold; font-size:16px">The green color means the experiment was a success



<font style="color:black;font-weight:bold; font-size:18px">Experiments ran :</I>

Part II : Functional testing of the part II
Ptet RFP Plac TetR was tranforme into Top10 bacteria and grew on media :

0,1% IPTG.

1% glucose.

LB pure.

To learn more, see the Conclusion page

<img style="width:40px; height:40px;" src="http://2009.igem.org/wiki/images/1/10/Paris_Up.png"/></a>

<div id="paris_content_boxtop"> <div id="paris_content">

The destabilization of the membrane
<font style="color:green;font-weight:bold; font-size:16px">The green color means the experiment was a success



<font style="color:black;font-weight:bold; font-size:18px">Experiments ran :</I>

<img style="width:40px; height:40px;" src="http://2009.igem.org/wiki/images/1/10/Paris_Up.png"/></a>