User:DavidC/22 August 2009

DNA extraction
Lambda phage electrophoresis after Lambda DNA extraction.

PCR
Sample: D protein.

Cycle :

94°C 2minutes; (94°C 1 minute; 50°C 1 minutes; 72°C 1 minute(s)) X 5; (94°C 1 minute; 60°C 1 minute; 72°C 1 minute(s)) X 35; 72°C B + 3 minutes;

DNA electrophoresis
85 Volt, 15 minutes. 105 Volt, 40 minutes. Ladder fermentas 1 Kb.

Samples: D protein (D prt)