Illinois/22 July 2009

July 22
Hybrid Promoter

The gel that was run to inactivate the digestion looked awful.



Reexamined protocol from the fermentas website and decided to attempt a double digest using Spe1 first and then EcoRI

Modeling

We retried PCRs for the pSB3K3 plasmid backbone, using two samples of template: Spring 2009 Plate 2 Well 15L and Spring 2008 Plate 1014 Well 1F. We ran four different PCRs, two for each under two different PCR programs: the program for amplifying PJU-334 and the program suggested for amplifying pSB3K3. Our gel indicated that none of the four PCRs worked.

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