Team:Washington/Accomplishments

=University of Washington 2009 iGEM Team Accomplishments=

Demonstrated that at least one new BioBrick Part of our own design works as expected

 * BBa_K215000 Target Expression Vector - expresses a favorite protein fused to streptavidin-binding Nano-Tag, secretion tag that directs export of the protein, and a His tag - all tags are removable by incubation with TEV protease. Find this in our wiki
 * BBa_K215091 Expresses OpdA, a nerve agent-degrading enzyme. Find this in our wiki
 * BBa_K215000 Protein Expression Vector - when IPTG-induced, expresses a protein with strong promoter and RBS (a good combination of BioBricks not previously found in the Registry). Find this in our wiki

Characterized existing BioBrick Parts and documented this information on the Registry

 * Find these in our wiki
 * BBa_J36848 2006 Harvard iGEM Streptavidin Cell Surface Display Construct - monomeric streptavidin, one OmpA transmembrane helix
 * BBa_J36849 2006 Harvard iGEM Streptavidin Cell Surface Display Construct - single chain dimeric streptavidin, one OmpA transmembrane helix
 * BBa_J36850 2006 Harvard iGEM Streptavidin Cell Surface Display Construct - monomeric streptavidin, five OmpA transmembrane helices
 * BBa_J36851 2006 Harvard iGEM Streptavidin Cell Surface Display Construct - single chain dimeric streptavidin, five OmpA transmembrane helices

Synthesized and BioBricked type I secretion system from Erwinia chrysanthemi

 * Find these in our wiki
 * BBa_K215107, Type I secretion system from Erwinia Chrysanthemi, constitutive expression, low copy, STRONG PROMOTER
 * BBa_K215108, Type I secretion system from Erwinia Chrysanthemi, constitutive expression, low copy, MEDIUM PROMOTER
 * BBa_K215109, Type I secretion system from Erwinia Chrysanthemi: constitutive expression, low copy, WEAK PROMOTER

Developed assay to quantify secreted protein

 * This assay assess for the presence of secreted His-tagged protein in the growth media by running media over a nickel column (His-tagged proteins will be retained), wash column, and elute protein. Eluted protein is quantified by comparing to a calculated standard curve.  Find this in our wiki

Designed new modular surface display system

 * This BioBrick is designed to express any protein as a cell surface display protein, using the well-characterized Lpp-OmpA cell surface display system. Generated BioBrick, functionality not confirmed.  Find this in our wiki

Worked on computational design of novel biotin-binding protein using Rosetta and Fold-It

 * Find this on our wiki

Gained real-world synthetic biology experience

 * We learned about the power of synthetic biology to address real-world problems, gained experience in designing and constructing our own molecular biology projects, and discovered that proficiency in troubleshooting is a must with synthetic biology. Find us in our wiki

=BioBricks=