September/21 September 2009

1.colony check sample no. no. of colony 27           ++    28            ++    30            ++    35            +     45            +    46            1    48            ++    50           +++    51            +   1-1F          +++ 46           1   2-9A           + 2-3I          ++ 3-7G          ++ 3-7C          ++ 1-8K         +++

2.transformation sample no 36, 37 , 38 , 39 , 40

3.min prep sample no. conc. 27          285 ng/uL 28          181   30           194   35           173   45           290   48           35   50           153   51           146  1-1F          132

4.digation

X1-A

X2-A

X3-A

X4-A

↓ 37°C, 2hr ↓ gel electrophoresis gel cut purification ↓ ligation O/N

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