Team:UNICAMP-Brazil/Notebooks/August 15

Biobricks recovered: RBS and CFP digestion

 * Today we started the construction of our first biobrick. After quantification of the miniprep products (from August 11) we performed the digestion (Protocol 14) with SpeI/PstI and XbaI/PstI of plasmids containing the RBS and the CFP reporter respectively in order to connect these parts.


 * After the digestion we isolated the target bands from the agarose gel, according to Protocol 7.




 * The CFP digested band was correctly purified from the gel. Unfortunatly, we lost the RBS fragment. =( It must have been lost at some stage of the purification, therefore we could not ligate these two parts today.

Raíssa