Team:TorontoMaRSDiscovery/19 May 2009

=May 19, 2009=
 * 1) 2L of TE buffer made (10X TE)
 * Recipe for 2L from stock solution (10X TE)
 * a) 10 ml 1M Tris-HCl pH 7.5-80.0 x 2 = 20 ml
 * b) 2 ml 0.5 M EDTA pH 8.0 x 2 = 4 ml
 * c) 988 ml ddH20 x 2 = 1976 ml
 * To make Tris-HCl, Tris base is used and adjusted to desired pH using HCl 
 * 1 M Tris base is required of which 20 ml is required – thereby 2.4228 g Tris base (due to scale limitations, 2.42g of Tris Base was used
 * 1) 500 ml of 1 M Tris Base made
 * mass of Tris used = 1M/1000 ml x 500 ml x 121.14 g/mol = 60.57 g
 * volume of water used = 500 ml
 * 1) 250 ml of 0.5 M EDTA solution was made
 * mass of EDTA used = 36.53 g
 * observations: EDTA did not dissolve in ddH2O on heat and being stirred