Team:Chiba/Notebook/Calendar/24 September 2009



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(23_September_2009 <|>25_September_2009)

Examine limit of AHL generation(1)-3
Yesterday's operation is here.

14:00
 * Today's operation

We made plates from each of mixture and 10 mL of LB-Amp, Cm solution medium.


 * Element of mixtures

Transformation(2)-2
Yesterday's operation is here.

11:15-
 * Today's operation

We picked colony(plux-GFP only) and cultured it.

15:50-

Main culture

20:30-

Mini prep.

And resultant DNA was saved in freezer.

Transformation(3)-1
ptet-GFP pSB1A2 BBa_I13522(Amp)
 * Plasmids

ptet-RFP pSB1A3 BBa_I13521(Amp)

ptet-CFP pSB1A2 BBa_I13600(Amp)

mRFP without Nco1 site(Cm)

JW1262
 * Competent Cells

21:50-

We cultured each plates.

Digestion Test
1 : ECFP(BBa_I6057)
 * Samples

2 : mCherry(BBa_E2060)

3 : V-YFP(BBa_K084003)

4 : mOrange(BBa_E2050)

5 : LacZ &alpha;(BBa_T9003)

6 : mRFP without Nco1 site


 * Doble Digestion


 * Doble Digestion's Master Mix


 * Single Digestion


 * Single Digestion's Master Mix

12:50

Warm up at 37 degrees Celsius

13:30

Gel electrophoretic analysis

To judge character of LuxR mutants(3)-1
We poured 1 mL of LB-Amp, Cm liquid medium in 96 deep well and added glycerol stocks.

22:10-

We cultured and shook it at 37 degrees Celsius.