Team:Kyoto/GSDD/Notebook/0824-0830



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To Do

 * PCR
 * Electrophoresis
 * Gel Extraction kit
 * 3-O-22
 * 1-O-2

PCR

 * 3-O-22 PCR product (1,2):1232bp
 * 1-O-2 PCR product (3,4):462bp

To Do

 * Restriction Enzyme Digestion
 * purification kit
 * ligation
 * Transformation
 * 3-O-22
 * 1-O-2

transformation

 * colony
 * p-2(1)2:none
 * p-2(4)2:observed

We are going to try transformation of p【2】(4)② again.

To Do

 * (use p【2】(1)② after 0826 ligation)
 * Transformation
 * (use 【2】(1)① after 0825PCR, Gel extraction)
 * Restriction Enzyme Digestion
 * PCR purification kit
 * Ligation
 * Transformation

transformation

 * colony
 * p-2(1)2:observed
 * p-2(1)1A:none
 * p-2(1)1B:observed

We are going to make master plates of these sample.

To Do

 * 2(4)1(0825PCR)
 * Restriction Enzyme Digestion
 * PCR purification kit
 * ligation
 * transformation

(0825PCRed)
 * p-2(1)1
 * p-2(1)2
 * p-2(4)2
 * Make master plates.

transformation
No colony was observed.

We are going to try transformation of p【2】(4)① again.