Team:Groningen/Notebook/22 September 2009

GVP Cluster
Several ON cultures did grow

Several plates did grow

All stored at 4°C

Metal Accumulation
 ICP-MS

ON starter cultures continued for creating biomass for As uptake experiments combined with promotor activation and methalotein binding. Use E. coli + control vector, E. coli + pArsR-RFP, E. coli + pLac-fMT Take OD600 samples every 1-1.5 hrs of E. coli + pLac-fMT. Induce E. coli + pLac-fMT at OD600 ~0.6 with 0.5mM IPTG.