Team:Todai-Tokyo/Protocols/Transformation

Transformation Protocol
From iGEM Plates 

 After ligation or from miniprep
 * 1) Add 15µl TE (Tris-EDTA, pH 8.0) to well containing part and pipette up and down to resuspend
 * 2) Take 1µl of the above solution and mix with partly thawed competent cells on ice
 * 3) Leave on ice for 30 min.
 * 4) Heat Shock for at 42ºC  for 45 seconds
 * 5) Return eppendorf containing cells to ice and leave for 5 min.
 * 6) Add 500µl LB and culture at 37C for 30 min.
 * 7) Spread on plate with appropriate antibiotic resistance
 * 8) Culture plate at 37ºC  overnight


 * 1) Take 1µl (if from miniprep)/5µl (if from ligation) of DNA solution and mix with partly thawed competent cells on ice
 * 2) Leave on ice for 30 min.
 * 3) Heat Shock for at 42ºC  for 45 seconds
 * 4) Return eppendorf containing cells to ice and leave for 5 min.
 * 5) Add 500µl LB and culture at 37ºC  for 30 min.
 * 6) Spread on plate with appropriate antibiotic resistance
 * 7) Culture plate at 37ºC  overnight