September/11 September 2009

1.colony check (ligation sample No.) (no. of colonies) 43                   +++            3-1M                    ++

2.ligation check UP  :  24A 24B 24C 24D 33A 33B 33C 1-23L ladder 29 2-18F 31C 32A DOWN : 34A 34B 34C 2-6E 35A 35B 1-2M ladder 40A 40B 1-6I 39 1-14N ligation OK! (but 35A, 39)

3.Min prep Result of Nanodrop concentration check (sample No.) (concentration) 14-A          14.8 14-B          16.4 22-A          16.9 22-B           8.8 41-A          31.0 41-B          22.0 42-A          22.8 42-B          17.7

4.digestion and ligation digestion with restriction enzyme

K204045

K204046

K204017

5.ligation check & gel purification UP  : 35A 1-2M 39 1-14N 27 1-6I 28 2-18H radder DOWN : 17 45  46 No.27 ligation OK! ligation sample No.17, 45 , 46 ↓ gel purification ↓ ligation 16 degree O/N

6. min prep  Result of Nanodrop concentration check (sample No.) (concentration) 24A           74.2 B           52.4 29            71.1     33A            59.3 B           41.8 34A           53.2 B           47.9 35B           34.5 40A           29.9 B           36.6

7.for ligation check digestion sample 14A 14B 22A 22B 41A 41B 42A 42B 24A 24B 29 33A 33B 34A 34B 35B 40A 40B

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