Team:UNICAMP-Brazil/Notebooks/September 3

Electrocompetent E. coli

 * Today we prepared solutions, materials and the pre-inoculum to prepare electrocompetent E. coli.

Hemolysin Operon Amplification

 * We did the PCR to amplify the hemolysin operon using the total DNA extration that we had made before. Our sequence have almost 4000bp so we used a extension time of 3 minutes, the annelling temperatures in Celsius degrees was 56, 54, 52 and 51. No DNA fragment was shown in the agarose gel.

Marcos

Terminator Biobrick

 * The terminator plasmid was quantified and we made another digestion, Protocol 14. We found by electrophoresis that it worked!



Wesley