Team:TorontoMaRSDiscovery/11 August 2009

=August 11, 2009=
 * 1) Digested 4, 5, Enc, C(E+P), C(X+S)
 * 2) *BB4 did not digest
 * 5, C is stored in fridge
 * 1) Calvin suggested increasing the concentration of enzyme and increase time of digestion due to thawing enzymes over weekend in fridge
 * 2) Miniprepped 1+2(1) with new kit, got great yield ~100ng/ul
 * 3) Gel extracted C (X+S) after it was CIPed
 * 4) Overnight ligation of Enc+C in PCR machine
 * 5) Calvin gave us 2 vials of RbCl cells in -80C
 * 6) *one time use only
 * 7) *thaw and add DNA