Team:TorontoMaRSDiscovery/8 August 2009

=August 8, 2009=
 * 1) BB1 transformants showed many colonies
 * 2) *plates (a plate full of colonies)
 * 3) *-> increasing heat shock to 90s increased efficiency (Note: 3x DNA used in this transformation)
 * 4) *Calvin mentioned he does heat shock for 120s
 * 5) *Calvin also suggested to increase tranformation time, ie. after adding DNA, hold on ice for an hour
 * 6) DH-5alpha replates all grew on the plates, demonstrating LB can be re-autoclaved and poured as plates
 * 7) *there was no difference between transformants directly plated after adding LB+glucose or incubating for an hour
 * 8) Miniprepped overnights (1+2) samle 1,2,4, and K