Team:Wash U/Calendar-Home/1 September 2009

Tuesday September 1, 2009
9/1 Minipreps

Did 2 digests of RBS30_cph8 with 1.5 ug each according to prefix-insertion instructions from the Silver lab- cut with EcoRI and PstI (A1 and A2)

Did 1 digest each of 1.5 ug of ompR_term_ompC_GFP (B) and ompR_term_ompC_pucB/A (C) as the vector for prefix insertion- cut with EcoRI and XbaI.

The Results of this digest are:

The first 4 lanes are the two rxns for RBS30_cph8. The top band is the one that was cutout (ab. 2.3 kb)

The next 2 lanes are ompR_term_ompC_GFP and the last two are ompR_term_ompC_pucB/A



Concentrations: of products after Gel purification:

A1) A2) B) C)

The products were ligated in the following ratios after gel purification for 1 hr at 22 deg C, followed by 20 min at 80 deg C:

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