August/28 August 2009

1. Conducted miniprep followed by nanodrop concentration check on the following: 10-A, 10-B, 10-D, 10-E. Results of nanodrop check are as follows: Conc. 260/280 260/230 10-A     35.2     1.98     1.76 10-B    20.7     1.82     1.59 10-D    37.3     1.90     1.84 10-E    23.7     1.94     1.76

2. Conducted transformation for the following ligated parts: (12), (13), (14), (15), (16), (18), (19), (20), (21). Also BioBrick part 1-6I was transformed as a positive control. The plated-out cultures were incubated at 17:30pm.

3. Prepared 42 plates of amphicilin-resistance LA culture medium.

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