Virginia Commonwealth/12 June 2009

Results

 * Data from plasmid purification (Miniprep)
 * Buffer EB was used instead of water and 500 microliters was used instead of 50 microliters so the DNA will have to be concentrated.
 * 114 microliters of buffer and 6 microliters of sample (20x dilution)


 * 100 microliters of buffer and 20 microliters of sample (6x dilution)

MandM 19:17, 12 June 2009 (UTC)

Tasks

 * Review Literature

Wetlab
MandM 19:01, 12 June 2009 (UTC)
 * Mini-Prep on the promoter.
 * Maxi-Prep on the reporter and the backbone.
 * Maxi-Prep yielded no DNA so the cells were re-cultivated overnight to perform 4 mini-preps on them tomorrow. All cells were re-cultivated over night to prepare for freezing.

Bussingkm 15:40, 13 June 2009 (UTC)