Team:Warsaw/Calendar-Main/22 August 2009

Assembly of endosomal detection operon Marcin Task 1:  Electrophoresis of plasmid isolated and subsequently digested in: 21.08.09 Results:   All samples of cro CDS on  pSB1A3 are without insert (samples 1-8) All ligations of p53 CDS were correct (samples A-D) Comment:  The most probable explanation of this situation is circularization of empty plasmid because I have forgotten to dephosphorylate vector using CIAP Task 2: Prepare the bacterial cultures for isolation of following construct:  BBa_C0040 with  BBa_B0032 +  BBa_R0080</a> on  pSB1A3</a> plasmid</li></ul>

Making BioBricks' stocks Justyna Task:  Making the glycerol stocks of bacteria bearing BioBricks.</li></ul> Following stocks were prepared: B0032+C0040, C0051+B0032, E0032+B0032, E0022+BOO32, P53+pSB1A3.</li> <BR>

Making of the plac-RBS-llo-intA part Jarek

Tasks:  Digestion of the the plac+RBS+llo and internalin A with PstI endonuclease.</li> Separation of digestion products in 0,8% agarose gel.</li> Cuting the agarose blocks containing the digested DNA from gel.</li> Preparation of pure DNA samples from agarose blocks with A&A GelOut kit.</li> Preparation of ligation containing digested internalin A and vector containing plac+RBS+llo part.</li> </ul>

Cloning switch 1 regulatory parts [ K177012</a>-PcI.RBS.LacI, K177033</a>-PcI.RBS.LacI.PcI.RBS.RFP.terminator, K177011</a>-PLacI.RBS.cI.terminator, K177038</a>-PLacI.RBS.cI.terminator.PLacI.RBS.GFP.terminator ] into two compatible low copy number plasmids of different antibiotic resistance Ania Tasks:  <li> </li> </ul>