Team:KULeuven/13 July 2009

= Project progress =

A & B (comparator), ideas
Key/lock system, see Riboregulators (Berkeley 2006)

Odor synthesis/sensor

 * Vanillin synthesis: DNA not yet complete/in library
 * Combination of 5 genes; 3 are available
 * Edinburgh Vanilla production (Synthesis pathway by Edinburgh igem 2007)
 * Vanillin production using metabolically engineered Escherichia coli under non-growing conditions, Ferrulic accid --> Vanillin, 5k bp

Light sensor

 * YcgF Gene, natural available in 3 E. Coli strains. Can use the promotor of YcgF gene for expression
 * Probable strain K12
 * Primer of YcgF Promotor:
 * AACAATCCAGGGTAATGGGTGAGGCGAGAGTAAGACGGTAACAGACATATCTTCTTG TGTCTTTCTTTTAATACCAAAACATAACCGTTTCTTTACATTGATAAAAAATGGAAAAAG TTGAACACTAGTTGGCGAAAAATCTTGTATAGATTGTCAGTTAAATGATGCAATATGTT TTATCATAACACATTGTTTTATATGCATTAGCACTAATTGCAAAAAATTAATTTATCATT CTGTACACATATTTCGTACAAGTTTGCTATTGTTACTTCACTTAACATTGATTAACATTTTTAACAGAGGCGTAGCATG (bron: [www.ncbi.nlm.nih.gov])

Odourless strain of E.coli

 * MIT 2006 used an indoline deficient strain of E.coli: YYC912.
 * Knockout of TNAa5- gene (tryptophanase) in various strains and selected the least 'smelly.'

=To do=
 * Blue light receptor
 * Decide on E.Coli strain
 * Try with K12
 * Vector in neural scent (indole knockout)
 * Decide on primers (!Pre + suffix!)
 * PCR of promotor
 * Couple promotor to GFP --> Standard iGEM plasmid
 * Test!
 * Obtain regulation site
 * Vanillin synthesis
 * Send mail to University of Tuscia
 * Presentations
 * Order receptor biobrick
 * Trg - Env2
 * PBP
 * PBP

= Drylab =

BioInformatics bookmarks

Wiki

 * Create Ideas page
 * Some idiot replaced the example logo Image:Example_logo.png with his own logo, so all references to it were removed from our wiki

= Progress of parts =