September/14 September 2009

1. colony check Checked the cell cultures transformed and plated out yesterday for colony formation and made an approximate count of the number of colonies.

(plate number)-(location on plate) (no. of colonies) 1-16I                      >50 1-1K                       >50 1-16C                      >20 1-4K                       >20

ligation sample No. 18                      >50 No. 41                      >50 No. 42                      >50 No. 45                      0 No. 46                      0

2.digestion for ligation check digestion with restriction enzyme

for sample No. 27, 28 , 29 , 30 , 35 , 36 , 39

3.DNA extraction 2-24I

4.ligation check and  gel purification UP  : for ligation sample No.17, 37 , 38 DOWN : ligation check No. 27, 28 , 29 , 30 , 35 , 36 , 39 ligation check : failure?

5.digestion and ligation sample No. 20, 29 , 35 , 27 , 28 , 29 , 30 , 36 , 39

K204020

K204029

K204035

K204027

K204028

K204030

K204036

K204039

6.transformation sample No. 14, 17 , 41 , 48 , 2-24I , 45 , 46 , 1-14N

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