Team:Gaston Day School/12 May 2009

 All 8 grew Spun 1.5 mL of overnight culture for 5 minutes in microfuge #1-4 left 5-8 o/n 37C Aspirated off all but 100microliters of the supernatant and resuspended the pellet by vortexing Refrig. o/n Make 2M NaOH (100mL)-- 8g/100mL = 2M 3m Na Acetate (100mL)--26.4g/100mL = 3M