Team:Warsaw/Calendar-Main/18 June 2009

Testing of BioBricks from 2009 distribution and pVDL9 plasmid Monika, Marek

 Isolation of plasmid DNA from 3ml of overnight bacterial cultures with Plasmid Mini kit (A&A Biotechnology) Restriction digest of minipreps with EcoRI and PstI (ca. 200ng of DNA, 0.3ul (3U) of each enzyme, 2ul of Orange buffer, H2O to final volume of 20ul) for 2h. Electrophoresis of digests in 1% agarose gel 

(place for the photo of the gel)