September/13 September 2009

1.Min prep Result of Nanodrop concentration check (sample No.)     (concentration) 3-1M              27.6 ng/uL No. 30           162.0 ng/uL No. 36            67.6 ng/uL

2.digestion and ligation

digestion with restriction enzyme

K204047

K204048

3.ligation check

UP : ligation sample No.47 No.48 DOWN : for ligation check 14A 14B 27 28 29 30 35B 36 39

No. 47, 48 gel purification & litaion 16 degree O/N ligation check : failure ???

4.transfromation for DNA modification : No.41, No.42 , 1-1K , 1-16I transformation      : No.45, No.46 , 1-4K , 1-16C 37 degree O/N

5.digestion and ligation

digestion with restriction enzyme

K204017

K204037

K204038

37 degree O/N

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