Team:TUDelft/Synthetic Transcriptional Cascade: The plan

=Synthetic Transcriptional Cascade: The plan=

Comparing advantages and disadvantages of both approaches (mainly the leakage problems), the negative synthetic transcriptional cascade was selected. The construction of the plasmids is as showed in figures 6 and 7.





After addition of IPTG, the repressor LacI will change its configuration; this will allow the transcription of TetR in plasmid 2. This molecule will act as repressor of the promoter Ptet in plasmid 2, this stops the production of the repressor cI and red fluorescent protein (RFP). After these events, the promoter lp will be free to allow the production of GFP after a delay in time from the addition of IPTG to the final expression of GFP (Figure 8).



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