IBB Pune/2 October 2009

We pay our respects to Mahatma Gandhi

Transformations:

1 LuxR plate 2 well 40 pSB1A2 2 Negative control 3 Ligation product of PR-TorA-GFP 4 Ligation product of PR-YcdB-GFP

Plasmid Isolations:

1 TorA in pSB1Ak3 2 Constitutive promoter-RFP 3 pSB1Ak3

PCR confirmations with VF2+Vr:

1 lac operator 2 TorA-GFP

3 YcdB-GFP 4 PR

5 pSBAK3 6 TorA

7 LuxI 8 pLuxR

9 fast GFP 10 PR-GFP (AK3)

11 pLuxR-LuxI 12 PR-GFP

13 T7P 14 TorA-GFP-PR

15 YcdB-GFP-PR 16 AK3

All the PCR confirmations were successful!