Uppsala-Sweden/15 October 2009

Triparental mating
The transfer of PRKRYT to cynecosystis were started today awaiting to be continued tomorrow.

Evaluating transformations
The colonies that were picked yesterday were evaluated on a gel.



(From left)

1. Fermentas 1kb ladder

2-6. PRPRYT(AC) 1-5

7-11. PRKRYT(XAK) 1-5

12. Fermentas 1kb ladder



(from left)

1. Fermentas 1kb ladder

2-6. PRPRAT(XAK) 1-5

7-11. PE1B3T(XAK) 1-5

12. Fermentas 1kb ladder

Inoculations of PRPRYT 2 & 4 and PRKRYT 4.

New colonies were pciked for the failing ones but with poor result again, as suspected. Only one colony for PRPRAT had the right length, and this one were inoculated as well.