Template:Team:KULeuven/31 August 2009/BlueLightReceptor


 * 1) A miniprep and RD (with EcoRI and PstI) were performed on the four colonies that were ented on Sunday.
 * 2) * Nanoprop results
 * 3) * RD results on gel: the right signals were detected
 * 4) LigA was plated on LB medium. This will be the "motherplate" from which the glycerol stock and the testing cultures will be taken from.
 * 5) Electroporation of  in competent cells. It was plated and put overnight in the incubator.
 * 6) In order to be able to grow vector, which contains the toxic ccdB gene, we need special cells which carry the gyrA462 mutation. This is strain DB3.1 from E.coli. These were plated and put in the incubator overnight.