Team:Warsaw/Calendar-Main/2 July 2009

Cloning of mitochondrial targeting signal Sebastian

Tasks:  Isolation of the pAcGFP1-Mito plasmid (containing mitochondrial targeting signal from subunit VII ot the human cytochrome C oxidase (Cox))

Methods:

Isolation of plasmid DNA from 1,5ml of overnight bacterial culture with Plasmid Mini kit (A&A Biotechnology). Detailed procedure of the isolation is described here Plasmid concentration in isolated sample - 50ng/ul