Team:LCG-UNAM-Mexico/Resources/Protocols/Transformation

Transformation Take 50micro liters of chemically competent cells (DH5alpha in our case) stored into a fridge at -70°C. Put the cells in ice. Add the plasmid to be transformed (The quantity is depending of the source and concentration). Let the samples in ice for 20 minutes. Apply a heat shock at 41°C for 1 minute. Incubate on ice for 5 minutes. Incorporate and mix well 1mL of liquid-LB medium. Mix at 250RPM for 1 hour. Centrifugue to concentrate for 1 minute at 13000RPM. Discard the supernatant. Plate into a petri box.