Uppsala-Sweden/3 August 2009

Glycerol Stock for Z. Mobilis
The test protocol works really fine as prelimary test results show. Thus I made final gylcerol stocks for Z. Mobilis.

The protocol can be found here: [[Media:Glycerol_Stock_Z_mobilis.xls]]

--Karl.brune 12:42, 3 August 2009 (UTC)

Buffers for transformation
We prepared the buffers (TE and SOB) needed for the transformation.

The protocols can be found here and --Flormane 12:13, 4 August 2009 (UTC)

PCR, Kivd, ADH2 and PpetE
We started a PCR reaction to extract the Kivd gene from Lactoctococcus Lactis sp.Lactis, ADH2 from Saccharomyces cerevisiae and the promoter PpetE from Synechocystis.

The primers used was [[Media:primers_090803_adh2_kivd_ppete.xls]] --Flormane 12:13, 4 August 2009 (UTC)