Team:Newcastle/Meetings/16 April 2009

Instructor Meeting 16 April 2009
Students: Jess, James, Hanny, Goksel Instructors:

Apologies: Craig, Matt, Jane, Arun

Agenda of the Meeting

 * More about flowchart/table/modelling
 * iGEM wiki changes
 * Europe Workshop: Most likely May 30/31 in London, UK at Imperial College.
 * Lethbridge - Bacteria over Batteries - bacterial battery that uses photosynthesis to generate electricity.
 * EPF-Lausanne - Brainstorm of 10 ideas.

Action Points of the Meeting

 * Need to show normal growth on diagram
 * Model population dynamics
 * Ask Nigel Robinson about Metallisation
 * Make powerpoint more detailed (biobrick slides)
 * Name promoters that we are going to use
 * Put them in the right order
 * Finding a homologue comK

Minutes of the Meeting

 * Knock out comK, and replace with one from another organism
 * Sigma D Motility, provides bi-stability
 * Work in a mutant background for biofilm formation, if we use sinI/sinR stochastic switch. Knocking out biofilm 'genes'
 * Use KinA
 * Methylase, no GFP. Instead of DNA rearrangement?
 * RNAs Restriction enzyme, instead of DNAase to prevent germination, but don't know how it will work
 * recA mutants, can repair themselves
 * Metallothiene incorporate with whole set of coat proteins of the spore
 * GFP and RFP with smtA to see where it binds on the coat of the spore
 * Knock out phosphotases rap. spo0E sporelation
 * Natural switches instead of synthetic ones
 * Streptococci Stochastisity Competence
 * Sigma F (spore specific) to control DNA rearrangement
 * Negative feedback to control smtA expression
 * Control mntH using mntR, also controls mntABCD, lets in Fe
 * Strong promoters - Sigma K, cotC, cotA - can be used for smtA expression
 * Sigma D is expressed all the time, provides bi-stability
 * May be able to use E-Coli biobricks for DNA rearrangement