Team:TorontoMaRSDiscovery/27 August 2009

=August 27, 2009=
 * 1) Digested 4+5 (1), (2), BB7 with E+P
 * 2) Ran digstes on gel
 * 3) *wall between lane 5 and 6 was broken upon loading
 * 4) religated BB3 digest and 3+2 into C plasmid for 2 hours at room temperature
 * 5) plated ligations: 2ul of ligation in 25ul of cells