Team:Paris/9 August 2009

NoteBook
August 9th  

Lab work
ON culture New transformation PCR
 * tri strain (for Caroline) 6ml LB
 * pSB2K3 in order to have new clones because I can't get the right digestion profil
 * NB: 1h20 of incubation at 37°C under agitation (Miam time --!)
 * For A11 (ex A4) ??name??: Matrix = Genomic DNA of K12, Oligo = O11 and O12
 * For A13 (ex A5) ??name??: Matrix = Genomic DNA of K12, Oligo = O1 and O13
 * 1:98°C 30s
 * 2:98°C 10s
 * 3:70°C 30s
 * 4:72°C 8s
 * Goto 3 29x
 * 5:72°C 10min
 * 6: 4°C ~

NB:1h at 98°C because I haven't push the "enter" buton after it warm up to 98°C...and all evaporate.  