UCL London/Protocol/Gel Electrophoresis

Gel Electrophoresis


 * Materials:


 * Agarose
 * TAE buffer
 * TAE buffer


 * Method:


 * 1) Weigh 1.50g agarose, and add 1×TAE to make up to 150mL.
 * 2) Cool down the agarose and add 15 µL ethidium bromide into the agrose.
 * 3) Pour the gel into the tray before it settles down.
 * 4) Spin the digested DNA for 5 sec.
 * 5) Add 5 µL orange loading buffer (glycerol & dye) to the lid; spin for few sec.
 * 6) Cover the gel by TAE.
 * 7) Load the gel and note down the sequence.
 * 8) At 110V, running for 30 min (or 1hr depends on the equipment).
 * 9) Check with UV light & take picture.