Wisconsin-Madison/5 June 2009

June 5, 2009
Ex 5: Results Triple Freezer Test is good



Ex 6: Sample 1: Results: Nothing from visible testing with match (video) Isopentenol is flammable and we expect a small poof or flame from the sample



Sample 2: Placed 2.5 ml of sample to into fresh 100ml LB, 50uL Amp, 73.5uL Cm, 100uL Tet OD 600

1. 0.078

2. 0.067

3. 0.077

4. 0.112

5. 0.146

6. 0.208	Induced with 1.02g Arabinose

7. 0.406 	Induced with 100uL IPTG

LET SIT OVER WEEKEND

Ex 7 results: Ran .7% Gel for verification of DNA, good



Primer: Spinach cDNA Primer Design

Other: research

Ex 8: Obtaining Additional Promotors: pSB1A3-1, BBa_J33210, rMMBRraA

1. Plated High copy BioBrick assembly plasmid

[]

pSB1A3-1 is a high copy number plasmid carrying ampicillin resistance. Well K1 of 2009 Spring Kit Plate 1 --- Plasmid pSB1A3 with part BBa_J04450

2. Broad host range BioBrick plasmid

[] BBa_J33210 - low to medium copy number, derived from pRL1383a, Streptomycin Spring 2009 Distribution, 2009 Kit Plate 3, Well 17B

3. Brian’s RFS1010 derived Plasmid - rMMBRraA

- transformed competent DH10B cells with pRL1383a and pSB1A3 (successful) - NP