Template:Team:KULeuven/3 September 2009/BlueLightReceptor


 * 1) A restriction digest on  with EcoRI and SpeI was performed. This will cut the promotor out of its vector  which has a RFP reporter gene. We will put  (cut with EcoRI and SpeI) in this vector. This way we will get a construct where RFP will be produced dependant on our blue light promotor. We can then compare this with the intensity of RFP produced by.
 * 2) Glycerol stock was made from LigA and . They are stored in the -80°C in S&P F8 67-68 and 69-70.
 * 3) Ecoli strain DB3.1 cells were made competent for electroporesis.
 * 4) Gel electorphoresis and extraction on