Team:Brown/Notebook Meetings/5-6-09

iGEM 2009 Meeting

May 6, 2009


 * 5:09: Meeting begins in SFH 218, with Adrian and Dana. Today we are presenting our powerpoints for Friday’s panel meeting
 * 5:09: Minoo: engineering yeast used to make breast to upregulate production 3 essential amino acid
 * A: LEU3 promotes transcription of genes that produces more amino acids
 * B: Increase production of pyruvate: amylase breaks down starch to produce more pyruvate; genes for this have already been made
 * C: packaging and secretion by ILVp gene – package into ILV rich proteins
 * Adrian: why don’t we find an endogenous protein in yeast that has high ILV content
 * Steal some pathways from Rice 2007


 * 5:29: Indu: solving allergies
 * Pro biotic – can we induce the protein secretion into the blood?


 * 5:59: CCDB – 3rd mechanism poisons DNA gyrase
 * It’s in the registry
 * Leaky promoters ? issue of cell dying when you want it to live
 * Find balance where expression of UCP is enough and where leakiness doesn’t affect cells
 * Selective pressure to keep the plasmid
 * If the UCP is not made then it will be mutated and not work