Virginia Commonwealth/27 July 2009

Results
Kevin & Adam

Maria and Afton Trentay 16:57, 28 July 2009 (UTC)
 * Nothing is glowing except one colony, J23110 w/ 702

Tasks
Kevin & Adam
 * Discuss and continue to study design principles for T7 and various other inducible/repressible promoters.

Maria and Afton Trentay 16:57, 28 July 2009 (UTC)
 * Regrow, Miniprep, Take spectrophotometry measurements, Digest, Ligate, Transform ligated parts:
 * pSB4C5, J23110, J06702,
 * Troubleshoot and Repeat this process until RFP is expressed
 * Make overnight cultures of parts pSB4C5, J23110, J06702, J23110 w/ 702

Wetlab
Kevin & Adam
 * Digest and Ligate synthetic promoter designs 1-9.

Maria and Afton Trentay 16:57, 28 July 2009 (UTC)
 * Overnight cultures were made (5mL of selective broth with 100 microliters of DNA)
 * Samples taken from cryogenic stocks
 * J23110 (AMP)
 * J06702 (AMP)
 * pSB4C5 (Cm)
 * Samples taken from plates
 * J23110 w/ 702