Team:Team:Imperial College London/Wetlab/Protocols/Cellculture/CC11

=SLIC= Sequence and ligation independent cloning (SLIC) was undertaken to join multiple pieces of DNA in a single step. The procedure also takes less time than standard ligation techniques. The cloning team did, however, experience limited success with this method.

The In-Fusion Advantage PCR Cloning kit from Clontech was used. The protocol (in .pdf format) can be found here.