Team:UNICAMP-Brazil/Notebooks/August 7

Genomic DNA Extraction for Hly Operon Manipulation

 * We performed the genomic DNA Extraction from four pathogenic E. coli hly+ strains. We used the Aubusel 1998 protocol with modifications, Protocol 1.

Marcos

Recovering the biobricks

 * 5 plates from yesterday transformation had colonies. The other 2 transformed bacteria were replated into two diferent antibiotics and in higher cell concentration, in the morning. At night one of these plates showed colonies.


 * We inoculated some of the grown colonies of each biobrick in liquid media to perform miniprep tomorrow.

Raíssa and Wesley