User:DavidC/18 October 2009

Tuesday the 13th to Sunday the 18th
Growth of bacteria in the goal to extract the bio-bricks:

BBa_J23100 + BBa_B0014; BBa_B0030 + BBa_J23100 + BBa_B0014; BBa_R0010 + BBa_B0030; BBa_P1003 + BBa_B0014; BBa_B0014 + BBa_P1003 + BBa_B0014; BBa_C0012 + BBa_B0014; BBa_B0030 + BBa_C0012 + BBa_B0014; BBa_I712669 + BBa_B0014;

5mL of LB medium + 5µL of correct antibiotic (kanamycin = 50mg/mL, ampicillin = 50mg/mL and tetracycline = 12,5mg/mL).

DNA extraction
By using miniprep from promega:

BBa_J23100 + BBa_B0014; BBa_B0030 + BBa_J23100 + BBa_B0014; BBa_R0010 + BBa_B0030; BBa_P1003 + BBa_B0014; BBa_B0014 + BBa_P1003 + BBa_B0014; BBa_C0012 + BBa_B0014; BBa_B0030 + BBa_C0012 + BBa_B0014; BBa_I712669 + BBa_B0014; D protein from lambda phage

Quantification
Quantification of miniprep with an electrophoresis. Mix : 2µL of loading buffer + 2 µL of DNA.

85 Volt, 15 minutes. 105 Volt, 40 minutes. Ladder fermentas 1 Kb.