Wisconsin-Madison/3 September 2009

September 3, 2009
miniprep digest and gel of potential clones

transformation of ProU/GsSDMT

'''nudF sequencing

.5 uL DNA (100 ng)

1 uL Big Dye

1.5uL Big Dye Buffer

.25uL Primer (fwd or rev)

5.75uL H2O

Sequencing clean-up using magnetic beads

Confirm sequencing results that nudF worked

Digestion confirmation of GSMT, photosystem promoter and pSB1A2