September/12 September 2009

1.colony check

(ligation sample No.) (no. of colonies) 24                     0            29                      0            36                      0            37                      0            38                      0

2.ligation and ligation check UP  :  24A 24B 29 33A 33B 34A 34B 35B 40A 40B marker 24 1-23L DOWN : 14A 14B 22A 22B 41A 41B 42A 42B marker 30 30 2-18F 36 1-14N ligation check : 24, 33 , 34 , 40 , 22 , 41 , 42 OK!

3.Min prep Result of Nanodrop concentration check (sample No.) (concentration) 27             147.2      28               96.1      39              108.8

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