EPF-Lausanne/14 July 2009

  

14 July 2009

Wet Lab
The digestion of the Terminator (BBa_B0010) was done, in order to purify it once more, using gel extraction.

Miniprep of LacI-RBS and Inverter TetR, and PCR of the two of them.

The linearized Terminator (BBa_B0010) was runned on an agarose gel and purified with a gel extraction kit.

Even though linearized Terminator (BBa_B0010) concentration was very low, we tried to ligate the previously amplified LOVTAP (08.07.09) in linearized Terminator (BBa_B0010)

Cloning Strategy
[[Media: primer15+160709.pdf| Primers designed for LOVTAP read-out and RBphP project.]]

People in the lab

 * Heidi, Tu, Nath, Caro

 