Team:Waterloo/Notebook/Protocols/Inoculation

 Inoculation Protocol 

Materials  DNA Samples Ampicillin (2µL/sample) Kanamycin (2.5µL/sample) Liquid LB Media (1 tube/sample)

Instructions  1. Label all tubes. 2. Do the following in the flow hood: - Pipette the correct amount of antibiotic into the corresponding DNA tubes. 2 µL for ApR, 2.5 µL KmR. - Dip the sterile stick into the DNA and into the corresponding media tube. 3. Incubate at 37 degrees Celsius overnight on the agitator.