Team:UQ-Australia/Notebook/Experiment2



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/*...............................................................................................*/ /* Menu Bar 1 Drop-Down Menu #2 Side Menu #1 div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1, div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1 { left: 15.5em !important; /* Places the side menu to the right of the drop-down menu. Keep it sync with the drop-down menu width. */ } div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1, div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1, div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1 li a, div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1 li a { width: 10em; /* Side menu width. */ } /*...............................................................................................*/ /* Menu Bar 1 Drop-Down Menu #2 Side Menu #2 div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2, div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2 { left: 15.5em !important; /* Places the side menu to the right of the drop-down menu. Keep it sync with the drop-down menu width. */ } div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2, div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2, div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2 li a, div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2 li a { width: 10em; /* Side menu width. */ } /*...............................................................................................*/ /* Menu Bar 1 Drop-Down Menu #2 Side Menu #3 div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3, div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3 { left: 15.5em !important; /* Places the side menu to the right of the drop-down menu. Keep it sync with the drop-down menu width. */ } div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3, div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3, div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3 li a, div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3 li a { width: 10em; /* Side menu width. */ } /*...............................................................................................*/

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Experiment 2
'''Transformation of pSUTp. pG.pmt and pSUTp'''

The bacteria used for this transformation reaction was DH5α Max efficiency cells strain (chemical competent) pSUTp. pG. pmt and pSUTp solution collected from 02/10/09 (click HERE fore further details)                            (Can you please put the link to 02/10.09 nanodrop result here?) were diluted to 10ng/uL solution to use for the transformation reaction.

Transformation control: pUC19
 * Click HERE for detailed protocol of transformation reaction.
 * While bacteria containing pSUTp.pG.pmt and pUC 19 were cultured on two separate Amp plates, bacteria with pSUTp was grown on Kan plate.
 * Incubate went overnight at 370C.
 * Results: Bacteria colonies were shown on plate in following morning
 * 08/10/09: Pick isolate colonies from pSUTp and pSUTp.pG.pmt cultures.

Click HERE for detailed protocol of picking colonies. NOTE: MerT and MerP primers arrived.
 * 4 colonies from pSUTp culture were picked and transfered into new tubes named pSUTp Kan 1-4
 * 4 colonies from pSUTp.pG.pmt were picked and transferred into new tubes named pSUTp.pG.pmt Amp 1-4