Team:Chiba/Notebook/Calendar/9 September 2009



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(8_September_2009 <|>10_September_2009)

08:00 main culture

 * divided injection 12.5ml of the culture to 10 50ml centrifuging tubes
 * WASH 3 times
 * Add 12.5ml new culture
 * Centrifuge the 1st tube 3000rpm 5min 20℃ and pick the upernatant
 * Do same operation every 15min to 2nd~10th tubes
 * after all, mix the supernatant and solid media at 1:1
 * make 10 concentration varieties of solid media