Team:Warsaw/TOC

Cloning of the mgtc promoter into the pKSII plasmid Cloning of hly gene into pKSII vector PCR inv EMSA isolation of genomic DNA from S. typhimurium PCR mgtc, pho PCR pho, mgtc, inv Testing of Biobrick parts obtained from the registry Preparation of chemocompetent E.coli DH5α</a> Testing of BioBricks from 2009 distribution and pVDL9 plasmid</a> Team meeting</a> Making Llo fusion with secretion signal peptide</a> Cloning of mitochondrial targeting signal</a> Quality check (cro PCR product and pKS isolate)</a> Cloning of p53 coding sequence</a> Informal meeting concerning important issues of the Project</a> Miecznikowa team Aim: debug RFP-terminator ligation that did not work</a> Creating devices to test promoters in E. coli strains (devices BBa_K177024 and BBa_K177025)</a> Isolation of BioBricks from 2008 and 2009 Kit Plates</a> Construction of K177012 operon1_part2</a> Insertion of the pho gene into the pKSII plasmid</a> Evaluating the quality of bacterial medium</a> Franek lub Monika opisze minilizy dla Moniki</a> Assembly of endosomal detection operon</a> <a href="http://2009.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=4&amp;arg0=16_July_2009&amp;arg1=17_July_2009&amp;arg2=18_July_2009&amp;arg3=20_July_2009&amp;name=Testing%20different%20E.%20coli%20strains%20regarding%20lacI%20and%20AraC%20repressors">Testing different E. coli strains regarding lacI and AraC repressors</a> <a href="http://2009.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=22_July_2009&amp;name=Assembly%20of%20invasion%20operon">Assembly of invasion operon</a> <a href="http://2009.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=22_July_2009&amp;name=Parts%20preparations">Parts preparations</a> <a href="http://2009.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=8&amp;arg0=22_July_2009&amp;arg1=23_July_2009&amp;arg2=24_July_2009&amp;arg3=25_July_2009&amp;arg4=26_July_2009&amp;arg5=27_July_2009&amp;arg6=29_July_2009&amp;arg7=30_July_2009&amp;name=Construction%20of%20K177012%20%20%20operon1_part2">Construction of K177012  operon1_part2</a> <a href="http://2009.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=23_July_2009&amp;name=PCR%20of%20Cox%20mitochondrial%20export%20sequence">PCR of Cox mitochondrial export sequence</a> <a href="http://2009.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=1&amp;arg0=24_July_2009&amp;name=Preparation%20of%20the%20electrocompetent%20bacteria">Preparation of the electrocompetent bacteria</a> <a href="http://2009.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=5&amp;arg0=27_July_2009&amp;arg1=28_July_2009&amp;arg2=29_July_2009&amp;arg3=30_July_2009&amp;arg4=4_August_2009&amp;name=Cloning%20of%20the%20mgtc%20promoter%20into%20the%20pSB1A3%20plasmid">Cloning of the mgtc promoter into the pSB1A3 plasmid</a> <a href="http://2009.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&amp;num=2&amp;arg0=27_July_2009&amp;arg1=28_July_2009&amp;name=Construction%20of%20RBS.3-listeriolysin">Construction of RBS.3-listeriolysin</a>