Virginia Commonwealth/4 August 2009

Results
Maria and Afton
 * Overnight culture of I1352 plated on AMP:
 * Sample taken from plate after PSB4C5 ligation attempt successfully grew expressing RFP. The color of the sample was pink
 * Sample taken from plate after ligation with itself did not grow at all, and expressed no RFP
 * There is a possibility the pSB4C5 backbone may be a bad part
 * previous years it has not functioned properly
 * Gel electrophoresis indicates DNA Gel Purification may be necessary
 * Possible inefficiency of BioBrick enzymes may have come from repeated freezing and thawing
 * They will be aliquoted into smaller amounts
 * Gel Electrophoresis Results

Trentay 22:25, 4 August 2009 (UTC)

Tasks
Maria and Afton Trentay 22:11, 4 August 2009 (UTC)
 * Run a Gel Electrophoresis
 * Transform and grow up pSB1C3 with the death gene P1010

Wetlab
Trentay 15:24, 5 August 2009 (UTC)
 * New BioBrick Assembly Kit came in and the following were aliquoted into their respective amounts
 * To show how many times a tube has been thawed and refrozen, a hash mark will be placed on the side of the tube, each time it is thawed as a reference for others to see

Maria and Afton Trentay 22:33, 4 August 2009 (UTC)
 * Gel Electrophoresis was run
 * pSB4C5 and I1352 digested and undigested (right after Miniprep) were run on a gel and results showed excessive bands in the undigested DNA. DNA Gel Purification will be done on future Miniprepped DNA
 * pSB1C3 w/ P1010 was removed from Well 5E on Plate 1 of the Distribution Kit and resuspended in 10µL of TE Buffer.
 * Transformation was done using DB 3.1