User:Annlesn

Ann's IGEM Notebook

[[media:7_22_09_and_7_23_09_Transformation_of_RBS_for_Pu_and_Killer_genes.pdf| 7/22/09 and 7/23/09]]


 * Comp cell Protocol
 * Protocol for getting biobricks from the registry
 * Transformation Protocol
 * Transformation of RBS for Pu promoter and killer genes from the biobricks

[[media:7_24_09_Miniprep_and_Digest_of_Pu_promoter_and_killer_genes.pdf‎ | 7/24/09]]


 * Miniprep protocol
 * Miniprep of RBS for Pu promoter and killer genes

[[media:7_25_09_Nano_Drop_of_RBS_and_Killer_genes_and_Digest_with_EcoRI_and_XbaI_of_RBS_and_Bactreriophage_lysis_cassette.pdf| 7/25/09]]


 * Nanodrop protocol
 * Digestion protocol
 * DNA concentrations for Pu promoter and killer genes
 * Digestion of RBS for Pu promoter and Bacteriophage lysis cassette from Brown 2008 with EcoRI and XbaI

[[media:7_27_09_Gel_of_RBS_and_Bacteriophage_lysis_cassette_digest_Plates_for_Pu_promoter_colony_PCR_Streaking_out_Pu_luciferase_part_from_the_registry.pdf‎| 7/27/09]]


 * Electrophoresis protocol
 * Gel of digested RBS for Pu promoter and Bacteriophage lysis cassette from Brown 2008
 * Sodium Benzoate Minimal Plate Recipe
 * Plates for Pu promoter colony PCR
 * Starting up Pu luciferase stab from the registry