Team:Alberta/References/Publications/Scarless engineering of the Escherichia coli genome

Tamas Feher, Ildiko Karcagi, Zsuzsa Gyorfy, Kinga Umenhoffer, Balint Csorgo and Gyorgy Posfai

Methods Mol Biol. 2008;416:251-9

Abstract: E. coli K-12, being one of the best understood and thoroughly analyzed organisms, is the workhorse of genetic, biochemical, and systems biology research, as well as the platform of choice for numerous biotechnological applications. Genome minimization/remodeling is now a feasible approach to further enhance its benefi cial characteristics for practical applications. Two genome engineering techniques, a lambda Red–mediated deletion method and a suicide (conditionally replicative) plasmid-based allele replacement procedure are presented here. These techniques utilize homologous recombination, and allow the rapid introduction of virtually any modifi cations in the genome.

Link: NCBI