Team:Kyoto/CiC/Notebook/1019-1023



 /* ul#navi_GSDD or ul#navi_CiC */ /* li.nav_i, li.nav_me, li.nav_g, li.nav_r, li.nav_p, li.nav_mo, li.nav_n */

background-position:left bottom !important; color:black; }
 * 1) navigation ul#navi_CiC li ul li.nav_n a{
 * 1) Home
 * 2) CiC
 * 3) Notebook

To Do

 * subgoal A --- Confirmation of function of TAT-liposome
 * observation by microscope
 * Subgoal B --- Liposomes with Mitochondrial Translocases
 * Mitochondoria isolation
 * expressing sig-GFP in vitro with mitochondoria
 * Subgoal C --- sig-EGFP expression in HeLa
 * transfomation

subgoal A --- Confirmation of function of TAT-liposome
Liposome was taken by HeLa cells.See the results.

Subgoal C --- sig-EGFP expression in HeLa
sig-EGFP　was made a localization in mitochondoria. See the results.

To Do

 * Subgoal B --- Liposomes with Mitochondrial Translocases
 * observation with microscope

Results
See the results.

To Do

 * subgoal A --- Confirmation of function of TAT-liposome
 * making liposome by natural swelling method and extruder
 * add liposome to HeLa cells

Results
According to the expectation,HIV-TAT-(LALAAAA)3-generator may work. Liposome is taken by HeLa Cells. See the results.