Team:UC Davis

 Homepage_UCdavis       <span style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"> </b></a><span style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"> </b><a href="http://2009.igem.org/Team:UC_Davis/About_Us"><span style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"> </b></a><span style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"> </b><span style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"> </b><span style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">   <a href="http://2009.igem.org/Team:UC_Davis/About_Us"><img alt="" src="http://2009.igem.org/wiki/images/d/d1/UCDAVIS_PIC4.png" style="border: 0px solid ; width: 92px; height: 46px;"></a>   </b><span style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">   <a href="http://2009.igem.org/Team:UC_Davis/Project"><img alt="" src="http://2009.igem.org/wiki/images/b/b9/UCDAVIS_PIC8.png" style="border: 0px solid ; width: 92px; height: 46px;"></a>   </b><span style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">   <a href="http://2009.igem.org/Team:UC_Davis/Notebook"><img alt="" src="http://2009.igem.org/wiki/images/2/2f/UCDAVIS_PIC5.png" style="border: 0px solid ; width: 92px; height: 46px;"></a>   </b><span style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"> </b><span style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">   <a href="http://2009.igem.org/Team:UC_Davis/Parts"><img alt="" src="http://2009.igem.org/wiki/images/a/a6/UCDAVIS_PIC6.png" style="border: 0px solid ; width: 92px; height: 46px;"></a> <a href="http://2009.igem.org/Team:UC_Davis/Contacts_References"><img alt="" src="http://2009.igem.org/wiki/images/1/1d/UCDAVIS_PIC7.png" style="border: 0px solid ; width: 92px; height: 46px;"></a>   </b><span style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"> <img style="width: 191px; height: 147px;" alt="" src=""> </b> <hr style="width: 100%; height: 2px;"><span style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"> </b> <span style="color: rgb(0, 0, 0);"> <span style="font-size: 13pt; line-height: 115%; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><span style="font-family: Symbol;"> <span style="font-family: Arial Narrow; font-weight: bold;"> </b><span style="color: rgb(0, 0, 0); font-family: Arial Narrow; font-weight: bold;"><span style="font-size: 13pt; line-height: 115%;"> <p class="MsoNormal" style="margin-bottom: 0.0001pt; line-height: normal;"><span style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;"> </b><span style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black; font-weight: bold;"> It is estimated that about one in every 133 Americans suffer from an autoimmune disorder called Celiac Disease (14, 11). Celiac Disease is a condition in which the small intestines of an affected individual cannot properly digest gliadin, a protein present in gluten. When consumed, gliadin induces immune system responses, the side-effects of which are, but not limited to: abdominal pain, chronic diarrhea, and vomiting (14). Currently, the only accepted treatment for this disorder is adhering to a gluten-free diet (14, 12). This can be very difficult as great varieties of food contain gluten. This inspired us to imagine an alternative treatment based on a probiotic organism that can survive and take residence in the stomach, where it would secrete an enzyme that degrades gliadin. <span style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"> Then the design elements of this organism should satisfy at least two main criteria. First, it should be able to survive only in the stomach; second, it should secrete an enzyme that degrades gliadin. We envision that these parts would not only be useful for our project, but also to other future projects. <span style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p> <span style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"> We decided to focus our summer project on building the first of the two key elements required for our system:<o:p></o:p> 1. <a href="http://2009.igem.org/Team:UC_Davis/secretion">Produce an inducible secretion system</a>  2. <a href="http://2009.igem.org/Team:UC_Davis/ph_sensor1">Construct a biological pH sensor limiting this secretion system to the stomach</a> <a href="http://2009.igem.org/Team:UC_Davis/ph_sensor1"><span style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><span style="text-decoration: underline;"> </a><span style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><big style="font-weight: bold;"> <p class="MsoNormal" style="margin-bottom: 0.0001pt; line-height: normal;"><span style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"> <a href="http://2009.igem.org/Team:UC_Davis/ph_sensor1"><b style="color: rgb(0, 0, 0);"><span style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"> </b></a><span style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"> <a href="http://2009.igem.org/Team:UC_Davis/ph_sensor1"><span style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><span style="text-decoration: underline;"> </a><a href="http://2009.igem.org/Team:UC_Davis/Project_Summary"><img alt="" src="http://2009.igem.org/wiki/images/a/ac/UCDAVIS_summary.png" style="border: 0px solid ; width: 63px; height: 50px;"></a><span style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"> <a href="http://2009.igem.org/Team:UC_Davis/ph_sensor1"><b style="color: rgb(0, 0, 0);"><span style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"> </b></a><span style="font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"> <b style="color: rgb(0, 0, 0);"><span style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"> </b> <hr style="width: 100%; height: 2px;"> <p class="MsoNormal" style="margin-bottom: 0.0001pt; line-height: normal; color: rgb(0, 0, 0); text-align: center; background-color: rgb(255, 255, 255);"><b><span style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"> </b><b><span style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"> </b><b><span style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"> </b><b><span style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"> </b> <ul style="background-color: rgb(255, 255, 255); color: rgb(0, 0, 0);"> </ul> UC Davis iGEM team is sponsored by: <img src="http://2009.igem.org/wiki/images/8/88/UCDAVIS_Biomedical_engineering.jpg" alt="" style="width: 83px; height: 109px;"><img style="width: 105px; height: 109px;" alt="" src="http://2009.igem.org/wiki/images/4/43/UCDAVIS_college_of_engineering.png"><img style="width: 135px; height: 99px;" alt="" src="http://2009.igem.org/wiki/images/8/8e/UCDAVIS_Accutek1.jpg"><img style="width: 121px; height: 109px;" alt="" src="http://2009.igem.org/wiki/images/6/6b/UCDAVIS_mrgen.gif"><img style="width: 182px; height: 108px;" alt="" src="http://2009.igem.org/wiki/images/c/c6/UCDAVIS_genome_center.jpg"> <font size="-1">We want to thank Marc Facciotti for his helpful advice. Special thanks to Suzy Fenton, Wayne Fenton, Jeanne Joe-Fenton and Wayne Fenton Jr. Also we thank <b>Charles H. Halsted </b> for reviewing our material regarding celiac disease. <ul style="background-color: rgb(255, 255, 255); color: rgb(0, 0, 0);"> </ul> <p class="MsoNormal" style="margin-bottom: 0.0001pt; line-height: normal; color: rgb(0, 0, 0); text-align: left; background-color: rgb(255, 255, 255);"><b><span style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"> </b> <span style="color: rgb(0, 0, 0);">