August/14 August 2009

miniprep
Conducted a "miniprep" to harvest and check concentration of plasmids from 14 sample.Result of Nano drop concentration check :(sample No.) (concentration) [260/280] [260/230]

R0071 (1-12C)  29.5ng/μL　 1.56  0.91 R0040 (1-6I)    7.5        1.88  2.79 R0079 (1-12A)  11.5       1.92  2.65 I14017 (1-18L)  28.6       1.81  2.46 B0034 (1-2M)   10.1       1.96  2.60 R0078 (1-16G)  14.0       1.99  2.47 C0171 (2-8I)    15.4       1.86  3.29 F2621 (2-21F)  17.9       1.99  2.60 I14015 (2-11N)  11.2       1.88  2.32 F1610 (2-24G)  19.5       1.97  2.34 C0161 (2-8O)   11.0       1.60  1.74 F2620 (2-6E)    5.5        1.46  2.03 K204001 - 3   16.1       1.78  2.40 K204001 - 8   14.4       1.77  2.38

transformation
to get new plasmid

sample No.    ③　④　⑤　⑥

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