Team:KULeuven/Wetlab

The labwork is divided into four groups, each working on a specific aspect of Essencia coli

Blue Light Receptor Purifying the promotor region and creating it into a new biobrick

Vanillin Production Testing the three enzyme (from Tyrosine to Ferrulic acid) and two enzyme pathway (from Ferulic acid to Vanillin) and eventually combining the five enzymes.

Vanillin Receptor Removing unwanted restriction sites, sequencing and combining all elements into a standard iGEM plasmid.

Key/Lock/Antikey Ligating Key, Lock and Antikey into promotor plasmids and testing the system with a GFP assay.