Wisconsin-Madison/3 June 2009

June 3, 2009
Other: 1. Made freezer stock of triple transformation and double transformation 2. Nate attempts to fix autoclave and went to lab and made new pipe 3. Prepare for advisor meeting tomorrow 4. Received Streptomycin in Mail

Primer: NudF Primer finalized Designing yHfR and ProU Primers

Ex 7: Isolation of Genomic from gram negative DNA from E.Coli K12 using Promega kit, Ptotocol for later isolation of Pro U operon and promoter Results: Spec DNA: 337mg/ml

- made freezer stocks of pNudF, pMBI, pMevT and pBAD33, pTrc99A transformed e coli - NP

- made a freezer stock of K12 and extracted its genomic DNA - NP