Team:TorontoMaRSDiscovery/3 September 2009

=September 3, 2009=
 * 1) only 1+2 (1), (4) showed growth in C
 * 2) Miniprepped Enc+5, 1+2 (1), (4)
 * 3) Digested Enc+5 with E,P, 1+2 (1), (4) with E,S, 3+2a with X,P for 1.5h
 * 4) Ran digests and old Tet digest from Monday on a gel
 * 5) *Forgot to add plasmid to controls
 * 6) *Tet digest still good
 * 7) *Enc+5 digest doesn't have ~900bp band -> not confirmed
 * 8) Started overnight ligation of 1+2+3+2/Tet
 * 9) Started overnight cultures: Ampx1, Kx2 Enc+5 x3, Tet, BB7