Virginia Commonwealth/1 July 2009

Results
Maria and Afton Trentay 20:29, 1 July 2009 (UTC)
 * E. coli cells DB3.1 with the plasmid backbone psB3k3 p1010 (death gene) had one visible colony
 * Positive controls have lots of cell growth
 * No visible colonies on the negative control

Tasks

 * Pick the colony
 * Have individual team meetings
 * Make new LB + Kan plates and broth

Wetlab
Maria and Afton Trentay 20:29, 1 July 2009 (UTC)
 * The one visible colony was picked and placed in 5mL of LB + Kan broth to incubate overnight.
 * The negative control that grew on the LB + Kan June 30, 2009 was replated on two Kan plates
 * one plate was made 6/8/09 and the other was made 7/1/09
 * If there is no growth on the most recent plates, then we know the previous Kan plates have degrated
 * If there is growth, someting is there giving the bacteria a resistence

Adam and Kevin
 * Adam and Kevin transformed and plated parts BBa_J23101, BBa_J23119, BBa_E0840, and pSB3K3 w/ P1010. See lab notebook page 1-3 for details