Team:Washington/Notebook/Standard curve

Generating a Standard curve for GFP concentration

 * 1) Purify GFP-fused protein using traditional purification protocol
 * 2) Measure absorption at 280nm to quantify protein amount
 * 3) Serially dilute protein into plate and read fluorescence, excitation 485nm, emission 525nm
 * 4) Compare values of protein concentration to fluorescence to create a standard curve allowing for the comparison of fluorescence to protein level