Team:TorontoMaRSDiscovery/22 August 2009

=August 22, 2009=
 * 1) Miniprepped overnights:
 * 2) *Tet1: Did 2 minipreps, one with normal protocol, one with low copy plasmid protocol
 * 3) **Normal protocol gave a higher yield
 * 4) *Tet with ampicillin: used low copy plasmid protocol, but cell pellet was much bigger therefore got the same yield as Tet1 normal protocol
 * 5) Digested the 2 samples with higher yields (500ng)
 * 6) Ran digests on gel, but still couldn't see ccdb gene
 * 7) Started to doubt restriction enzyme function, therefore redigested Tet H (1ug) and C plasmid (500ng)
 * 8) (1.5h digests for all digests today)
 * 9) Ran Tet H and C digests on a gel
 * 10) *A faint ~700bp band was observed in the Tet lane
 * 11) *More plasmid should be used for plasmid digests
 * 12) Started overnight ligation of 4+5 in Tet backbone