Team:Chiba/Notebook/Calendar/25 September 2009



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(24_September_2009 <|>26_September_2009)

To judge character of LuxR mutants (3)-2
Yesterday's operation is here.

10:45-
 * Today's operation

We transplanted E.coli by 48 pins to NC filter and cultured it.

22:20 measurement start
We transplanted E.coli, which has been cultured on NC filter, to solid medium which contains each concentration of AHL.

AHL concentration is : 0, 1, 10, 100, and 1000 nM

We decided this time is T=0 and observed condition of fluorescence every 30 min.


 * Mutants' Location

Pictures are here.

Examine limit of AHL generation(2)-1
11:05-

We did prior culture.

glycerol stock : plux-GFP with pCIA3-LuxR

23:35-

We attenuated culture solution 1/105 and dispersed on plate.

Then we cultured this plate at 37 degrees Celsius.

And We made plates from each of mixture and 10 mL of LB-Amp, Cm solution medium.


 * Element of mixtures

E. coli Painting (3)
We draw various pictures with ink brush.

ptet-GFP, ptet-RFP, ptet-CFP
 * Bacteria which used as color

23:45-

We cultured painted plate at 37 degrees Celsius.

Picture is here.