Duke University/9 June 2009

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June 9, 2009
Gel Extraction
 * 1) Weigh cut gel piece(s). Add at least 1 ul binding buffer per mg of gel.
 * 2) Place tube at 50-60°C for 10-15 min or until gel is completely dissolved. Vortex every few minutes.
 * 3) Add solution to spin column. Centrifuge at max speed for 30s. Discard filtrate.
 * 4) Add 300 ul binding buffer to column. Centrifuge for 30s. Discard filtrate.
 * 5) Add 500 ul wash buffer. Centrifuge for 30s. Discard filtrate.
 * 6) Repeat step 5.
 * 7) Centrifuge tubes for at least 2 min. Discard collection tube.
 * 8) Obtain 1.7 ml centrifuge tube. Add 40-50 ul elution buffer to spin column. Centrifuge for 1 min. Discard spin column.


 * E: Measured concentration of DNA fragments
 * R: Very low concentrations of fragments 1 and 3
 * C: Do PCR with fragments as template, greater volume to increase amount
 * E: PCR with fragments 1 and 3 as templates