Imperial College London/Notebook/16 September 2009

Protocol considerations

 * Overnight cells were diluted in fresh media 1:12
 * The cells were allowed to grow until their OD reached around 0.6 (around 4 hours)
 * IPTG was added from a stock solution of 1M IPTG and 0.1M IPTG.  Note that IPTG takes some time to defrost
 * 180ul of Media with relevant IPTG conc was added to each 96 well plate. 20ul of cells were added.
 * Put in the plate reader overnight using protocol IGEM Fluor Abs (do not use)

Results
[[Media:Lac-RFP 1609.xls| Download raw data]]

Conclusions

 * There was no change in the cell growth rate with IPTG
 * Since a Lac-RFP construct was used, this shows that IPTG, for both its toxicity and protein production stress, does not have much effect on cell growth at the concentrations tested

Suggestions/Improvements
After overnight readings, the regular absorbance readings were obtained, but no fluorescence readings were obtained. Therefore, the results had to be converted to a normal cell growth analysis.
 * The script merged the fluorescence readings into the absorbance readings.
 * Try to give the script test runs before leaving them overnight, and check on results regularly if possibly