Virginia Commonwealth/10 July 2009

Results
Afton and Maria
 * Overnight culture was successful
 * Miniprep/Spectrophotometry Results

Trentay 13:13, 10 July 2009 (UTC)

Craig and Clay
 * The overnight culture of the three parts related to limonene synthesis were unsuccessful. Possible reasons include bad parts, errors in electrotransformation procedure, and incorrect resistance.

Tasks
Afton and Maria Trentay 13:13, 10 July 2009 (UTC)
 * Make stocks of parts J23106 and J06702
 * Miniprep of parts J23106 and J06702
 * Digestion of parts J23106 and J06702, (and J23100)
 * Electrophoresis test of parts J23106 and J06702
 * May Ligate parts with backbones pSB1C3 and pSB4C5 and store DNA
 * Write out complete plan for experimentation
 * Make plans to MaxiPrep
 * Write out a plan to organize ideas

Trentay 17:05, 13 August 2009 (UTC)

Craig and Clay
 * BBa_I742111, BBa_K118024, and BBa_K118025 need to be plated again.
 * If the new plates also show no growth, electrotransformation of the parts will be performed again.
 * If this proves to be unsuccessful, the cells from this second electrotransformation will also be plated a second time. The cells will also be plated on non-resistant media.
 * If growth is again unsuccessful, the cells grown on the non-resistant media will be streaked on kanamycin and chloramphenicol plates.

Wetlab
Afton and Maria Trentay 20:35, 10 July 2009 (UTC)
 * Dilution of overnight culture
 * 2 mL of overnight culture was added to 4 mL of LB broth (6 mL)
 * 2 dilutions were done for each test tube
 * Cells not used in the MiniPrep will be stored in the -80 degree Celsius freezer
 * 500mL of 30 percent Glycerol stock was made
 * Cell stocks were made
 * 10 vials of J06702 were put in the -80 degree Celsius freezer
 * 5 vials of J23106 were put in the -80 degree Celsius freezer
 * MiniPrep was done on parts: J23106 and J6702
 * Amount of DNA was calculated with a Spectrophotometer
 * Digestion was performed on MiniPrepped parts
 * There are two digested J06702 PCR tubes
 * Digests will be frozen until Monday
 * Electrophoresis test will be held off until then
 * Ligation as well

Craig and Clay
 * The electrotransformed parts were plated again (ampicillin plates) due to unsuccessful growth. Fifty microliters was used instead of 35 microliters.