Team:Imperial College London/Wetlab/Protocols

= Protocols =

Cell culturing/cloning protocols

 * CC1: Miniprep
 * CC2: Midiprep
 * CC3: Making cells competent
 * CC4: Ligation
 * CC5: Transformation into BL21 using electrical shock
 * CC6: Transformation into Top10 using chemical competence
 * CC7: Making LB plates
 * CC8: Extracting DNA from the registry
 *  CC9: Preparation of XL1-Blue electrocompetent cells & XL1-Blue quality control
 * CC10: PCR
 *  CC11: SLIC
 *  CC12: Biobricking parts from oligos
 * CC13: Genome Prep for Restriction Assay

Calibrations

 * CA1: Optical Density Calibration
 * GFP fluorescence calibration
 * CA2: External GFP fluorescence
 * CA3: Intracellular GFP fluorescence

Promoter Characterisation

 * PP1: Lac Promoter Characterisation

Autoinduction

 * AI1: Secondary Carbon Source Experiment
 * AI2: Glucose Time Delay

Protein Production

 * PP1: IPTG Toxicity
 * PP2: IPTG characterisation
 * PP3: Cellulase
 * PP4: PAH

Encapsulation

 * EN1: Colanic acid production amount
 * EN2: Colanic acid protection from pH
 *  EN3: Trehalose production

Killing

 * KI1: Thermoinduction
 * KI2: Restriction Enzyme activity