Team:UNIPV-Pavia/Notebook/Week4Sep

 = Week from September 21st, to September 27th, 2009 =

September, 21st

 * Wiki updating

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pH sensor

 * LB NaCl 70 mM pH 5,5 - 6,6 - 7,5 - 8,5 + Amp preparation.
 * Infection of 4 ml LB + Amp with 8 ul from glycerol stock of:
 * RBS33
 * A2
 * pNhaA
 * Overnight incubation of these three cultures at 37°C, 220 rpm.

Experiment with Tecan F200
 * Download Protocol

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pH sensor
2nd experiment
 * We put 50 ul into 5 ml LB NaCl 70 mM + Amp pH 6,6 falcon for each of the overnight cultures RBS33 - A2 - pNhaA and incubated them for four hours and a half at 37°C, 220 rpm.
 * OD measure of the three falcons with TECAN in order to make dilutions with a final volume of 3 ml and an OD equal to 0,02.
 * We diluted:
 * RBS into:
 * LB NaCl 70 mM + Amp pH 5,5
 * LB NaCl 70 mM + Amp pH 6,6
 * LB NaCl 70 mM + Amp pH 7,5
 * LB NaCl 70 mM + Amp pH 8,5
 * pNhaA into:
 * LB NaCl 70 mM + Amp pH 5,5
 * LB NaCl 70 mM + Amp pH 6,6
 * LB NaCl 70 mM + Amp pH 7,5
 * LB NaCl 70 mM + Amp pH 8,5
 * A2 into:
 * LB NaCl 70 mM + Amp pH 5,5
 * LB NaCl 70 mM + Amp pH 6,6
 * LB NaCl 70 mM + Amp pH 7,5
 * LB NaCl 70 mM + Amp pH 8,5
 * We started this 6 hours' experiment that performed absorbance and fluorescence measures (on three different wells) for each sample every 5 minutes.

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September, 24th

 * Fermentation experiment setup
 * Wiki updating

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pH sensor

 * Preparation of LB NaCl 151 mM at pH 5,5 - 6,6 - 7,5 - 8,5.
 * Preparation of LB NaCl 250 mM at pH 5,5 - 6,6 - 7,5 - 8,5.

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September, 26th

 * Fermentation experiment
 * Cloning
 * Wiki updating

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pH sensor

 * Infection of 4 ml LB + Amp with 8 ul from glycerol stock of:
 * RBS33
 * A2
 * pNhaA
 * Overnight incubation of these three cultures at 37°C, 220 rpm.

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