Virginia Commonwealth/11 August 2009

Results
Kevin and Adam
 * I have cell growth on all plates. Yesterday's work was successful.  Colonies number from 2-5 colonies per plate at 4:00 PM.  I will wait until later to pick a colony.

Maria and Afton Trentay 19:17, 11 August 2009 (UTC)
 * 1kb ladder has not come in yet so, digests and minipreps will be run on gels when it comes in
 * Plated colonies from Friday (8/7/09) transformations are strongly espressing RFP
 * It might be better to pick colonies 3 to 4 days after transformation when colonies are strongly espressing RFP, instead of when the e. coli are pink the day after transformation
 * Spectrophotometry Data

Tasks
Maria and Afton Trentay 14:43, 11 August 2009 (UTC)
 * Take Spectrophotometry measurements of pSB1C3 p1010, J06702, J23100, J23102, J23103, J23104
 * Need to locate J23100 to be digested another day
 * Digest above parts
 * Ligate J06702 and pSB1C3 with the following promoters: 100, 102, 103, 104
 * Transform parts into NEB10β
 * Update Wiki
 * Make overnight cultures of I1352/pSB1C3
 * I1352/I1352 will not be kept because it never expressed RFP and there are plenty of stock of I1352 with Amp resistance
 * Make overnight cultures of pSB1C3 p1010 from cryogenic storage to Miniprep tomorrow
 * Make more LB+Cm plates

Wetlab
Maria and Afton Trentay 20:12, 11 August 2009 (UTC)
 * Spectrophotometry was done
 * Digest was done
 * Ligation was done
 * Transformation was done
 * Overnight cultures were made
 * LB+Cm plates were made

Adam and Kevin -Bussingkm 21:16, 11 August 2009 (UTC)
 * The growth on the plates remains slow. No colonies show fluorescence yet, for the sake of better selection I am going to wait until early tomorrow morning to pick colonies.  I will cultivate them tomorrow and then store them in the evening.  Hopefully we will be able to measure transcription levels Thursday.