Wisconsin-Madison/14 September 2009

September 14, 2009
'''Colony PCR Gssdmt and GSMT

12.5 uL Master Mix

.5uL     Fwd Primer

.5uL     Rev Primer

11uL    DNA in H2O

'''Cycle

95C  5min

35*:

95C  30s

53C  155s

72C  60s

72C  5min

4C    Forever