Imperial College London/Notebook/30 September 2009

Nuri: -Did lacI/lactose diagram - Will read papers and continue CSS tutorial

Harvard GFP

 * Problems:
 * Fluorescence was saturated for our positive control


 * Conclusions:
 * Fluorescence for the shifted sample (SS) shows increase of fluorescence overtime indicating that the temperature sensitive promoter (pLambda cI) is activated when shifted to 37°C
 * However we could not determined whether the shifted sample reached the fluorescence level of the positive control as fluorescence readings were saturated on the fluorometer.
 * The non shifted sample (kept at 28°C) was constant at fluorescence levels similar to the blanks. This suggests that the promoter repression is tight at 28°C.
 * Blanks were constant