August/24 August 2009

1. LB+Amp plate

2.Min prep Result of Nanodrop concentration check

(sample No.) (concentration) 7-1         27.8   ng/uL 8-1         23.2      9             21.1      10-A         29.1 10-B        25.1 11-A        15.7 11-B        34.1

3.transformed check Checked the cell cultures transformed and plated out yesterday (8/23) for colony formation and made an approximate count of the number of colonies.

(sample No.) (no. of colonies) 1-4E           ~5 1-8E            0 2-16L           1 2-16N          ~15 2-18H           2 ligation sample 12            0       13             0       14             0       15             0        5             0  to iquid medium(5mL + Amp 5uL)

4.ligation check

↓ 37, 2hr ↓ gel electrophoresis

result 7 (RBS+cinR) 7-1    OK 8 (RBS+LuxI) 8-1    OK 9 (RBS+LasR+terminater) 9      fialure? 10 (RBS+EspE+terminater) 10    ??? 11(p(tetR)+RBS+GFP+terminater) 11-B   OK

No. 9 10 → ligation rapid check

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