September/7 September 2009

1.ligation 16degree 3hr ↓ transformation sample No.27, 28 , 29 , 30 , 1-1D , 9 , 10-D,E , 24 37degree O/N

2.ligation check for yesterday sample 23-A,B, vector , 22 , 25-A,B,C,D , vector , ladder ligaton OK

3.Min prep result of nano drop sample No. conc. 5           14.9 ng/uL 18           14.6 ng/uL 26           11.1 ng/uL

4.digestion and ligation digestion with restriction enzyme

K204009

K204024

K204031

K204032

↓ 37degree, 3hr Agarose Gel Electro-phoresis No.9-I,V, No.24-I,V , No.31-I,V , No.32-I,V gel cut purification ligation

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