Team:LCG-UNAM-Mexico/Resources/Protocols/Ligation

To ligate two biobricks into a base vector. Part 1: 	2 µl Part 2: 	2 µl Destination Plasmid: 	2 µl 10X T4 Ligase Buffer: 	2 µl T4 Ligase: 	1 µl H2O: 	11 µl

Incubate at room temperature for 10 minutes and then inactivate the enzyme at 80°C for 20 minutes.

Transform 5 μl of the ligation product into 50 μl of competent DH5-alpha E. coli cells.