Team:Newcastle/Labwork/14 October 2009

=Formal Lab Session - 14th October 2009=
 * This morning we check yesterday's transformation result, it turned out we got transformed cell for all three strains.
 * we patched the colonies on LB+EM+CHL+Starch plate to check whether the pGFP-rrnB:kinA part were integrated in amyE part or not.
 * After 6 hours grow on antibiotic and starch plate, we got some right colonies.



Microscopy Test to characterize KinA
We inoculated 6 cultures for the microscopy test from haloless colonies since tehy were transformed with our biobrick. We inoculated them into MM competence medium overnight


 * 1) Colony 1 from BFS2426 transformation
 * 2) Colony 18 from BFS2426 transformation
 * 3) Colony 13 from BFS40 transformation
 * 4) Colony 18 from BFS240 transformation
 * 5) Colony 19 from BFS867 transformation
 * 6) Colony 22 from BFS867 transformation
 * 7) Colony 5 from BFS2426 with halo. (As a negative control)
 * 8) No cell (Control)

Transformations
We did transformations from midipreps to have stock for our biobricks. We will freeze them later. We transformed ara, sac, ara+sspB Biobricks.