Imperial College London/Notebook/18 September 2009

Protocol considerations

 * The cells were grown for a day in medium containing FPA- presumably coated with colanic acid
 * Solutions of pH 1, 2, 3, 4 and water were prepared
 * Make 96 well plate layout
 * 200ul of each solution used as blank
 * 150ul of each solution added first, then 50ul of cells were added. Try to make addition of cells as close together in time as possible
 * Immediately placed in plate reader- using script pHTop10 180909x2 (3 min measurement intervals)

Results
[[Media:pHGFP_18-09.xls| Download raw data]]