Template:Team:KULeuven/19 September 2009/VanillinProduction


 * Miniprep PAMS
 * Restriction digest PAMS with S/P
 * Restriction digest COMT 13, 11, 21, 31, 41, 42 with X/P
 * Ligation PAMS&CO (Promotor, Sam5, Sam8, ComT)
 * Again one part from gel extraction (UV)
 * One part directly after restriction digest
 * Electroporation PEFT and PEFT (UV), both twice.