Wisconsin-Madison/25 August 2009

August 25, 2009
Sequencing Reaction on Gssdmt

.5 uL DNA (100 ng)

1 uL Big Dye

1.5uL Big Dye Buffer

.25uL Primer (fwd or rev)

5.75uL H2O

Sequencing clean-up using magnetic beads

Confirm sequencing results that Gssdmt worked.

'Cyanobacteria
 * optimum OD 685 - PCC 7002
 * reinoculated all samples in 5:1 ratios