Team:Chiba/Notebook/Calendar/26 September 2009



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(25_September_2009 <|>27_September_2009)

Examine limit of AHL generation(2)-2
Yesterday's operation is here.

11:35-
 * Today's operation

We transplanted E.coli to NC filter and put these on each plates.

And then we took pictures every 30 min.

Picture are here.

PCR
1 : ECFP(BBa_I6057)
 * Template

2 : mCherry(BBa_E2060)

3 : V-YFP(BBa_K084003)

4 : mOrange(BBa_E2050)

5 : LacZ &alpha;(BBa_T9003)

6 : mRFP without Nco1 site

14:45

We drew up mixtures for PCR.


 * Element of mixtures

15:20-

PCR

Gel electrophoresis
15:30

We made gel for electrophoresis.

agar 0.14 g

TAE 20 mL

17:25-

Gel electrophoresis(135 V, 27 min)