Team:Illinois/MicA

Back to sRNA Library Team page

MicA
'''Primers Used: for the sRNA gene we used Forward Primer: GAA AGA CGC GCA TTT GTT ATC Temp: (4*9) + (2*12) = 60 degrees Second homology sequence: TTC CAG CCA CAC CGC AAA CGG ((TTCGGTATCA)) Reverse complement: CCG TTT GCG GTG TGG CTG G Temp: (4*13) + (2*6) = 64 degrees Cut site and overhang: GTTTTT TCTAGA Reverse Primer: GTTTTT TCTAGA CCG TTT GCG GTG TGG CTG G

For the Target Sequence we used the primers from the Urban and Vogel paper: JV-0432 and JV-0433 '''

June 16
We used PCR to extract the sRNA gene: MicA and target sequence : ompA. from the e.coli chromosome. We then ran a gel to make sure that we had the right DNA fragments. Our results corresponded to our predictions.



June 17
We are completing a digestion of MicA, OmpA, and OmpF. Following the digestion we will incubate with SAP and then run a gel to verify the digestion. We will then extract the DNA for the sRNA target sequence and sRNA gene.