Virginia Commonwealth/15 July 2009

Results

 * Results of Retransformation with pSB4C5 backbone in NEB 10 beta


 * Controls with NEB 10 beta

Trentay 16:59, 15 July 2009 (UTC)

Craig and Clay
 * The ampicillin plates again showed no growth. However, the non-selective plates displayed lawn growth.

Tasks
Maria and Afton Trentay 16:59, 15 July 2009 (UTC)
 * Make more electrocompetent NEB 10 beta
 * 500 mL LB autoclaved in 1 L flask
 * 1 L LB autoclaved
 * 2mL of NEB 10 beta overnight culture was diluted in 4mL of LB broth
 * Two bottles of LB Agar were prepared
 * for Amp and Cm plates
 * Three bottles of LB prepared
 * two liquid LB+Amp will be prepared
 * one liquid LB+Cm will be prepared
 * Let transformed BioBrick parts continue to grow overnight (on plates) before colonies are picked
 * Make cell stock of Biobrick part: J23106, J06702, pSB4C5
 * 2mL of BioBrick overnight culture was diluted in 4mL of LB broth
 * Organize Notebook(s)

Craig and Clay
 * The cells growing on the non-selective media will be streaked onto plates containing kanamycin and plates containing chloramphenicol.

Wetlab
Maria and Afton Trentay 14:13, 16 July 2009 (UTC)
 * All LB was prepared and autoclaved
 * Overnight culture dilutions were completed
 * Electrocompetency of NEB 10 beta was completed
 * LB and Sterile water was made
 * 10% glycerol stock was made

Kevin & Adam
 * run gel on digestion from yesterday assembly.

Craig and Clay
 * Cells possibly containing the limonene synthesis related parts grown on the plates containing no antibiotics were taken and plated onto kanamycin-selective and chloramphenicol-selective plates.