Team:PKU Beijing/Notebook/AND Gate 2/T3

Notebook > AND_Gate_2 > T3

2009.7.30
12:30 T7 Polymerase store the strain 13:00 T3 Promoter + GFP Double digestion check 13:00 T3 Promoter Parts Double digestion check 13:45 T7 Polymerase Miniprep

2009.8.1
15:00 T7 Polymerase + 1-2M check

2009.8.5
18:20 T3 Promoter PCR

2009.8.6
21:30 Gel recycle of T3 Promoter PCR 23:45 T3 Promoter PCR Product Double digestion

2009.8.7
11:00 Purify of T3 Promoter Digested product 11:00 Three Counter Plasmid Miniprep 15:30 T3 Polymerase High pure PCR 18:00 Gel recycle of T3 Polymerase PCR

2009.8.8
11:00 Bi-stable Miniprep 12:00 Bi-stable Plasmid Double Digestion

2009.8.9
21:00 Ligation of T3 Promoter (insert) and PSB1K3 (Vector) 21:15 T3 Polymerase PCR Product Double Digestion

2009.8.10
6:00 T3 Polymerase Digestion Product Purify 6:30 Ligation of T3 Polymerase (insert) and PSB1K3 (Vector) 10:00 Transformation of T3 Promoter 22:00 Transformation of T3 Polymerase

2009.8.11
4:00 Sake the T3 Promoter Parts 4:00 PCR of T3 Promoter Parts 16:30 T3 Promoter Parts Miniprep

2009.8.13
13:30 Miniprep of T3 Polymerase 14:30 Double digestion of T3 Polymerase plasmid

2009.8.15
12:00 Minioreo of T3 Polymerase 14:15 Double digestion of T3 Polymerase plasmid 15:00 Sake the T3 Promoter Parts 20:00 Sake the T3 Polymerase

2009.8.16
14:15 Miniprep of T3 Promoter Parts 16:00 Miniprep of T3 Polymerase

2009.8.17
10:30 Double digestion of T3 Promoter Parts and T3 Polymerase 11:00 Miniprep of 3 Counter Plasmid and T3 Polymerase 22:00 Sake the T3 Polymerase

2009.8.18
12:00 Miniprep of T3 Polymerase 15:00 Double digestion of T3 Polymerase plasmid

2009.8.20
10:00 PCR of T3 Polymerase 14:00 PCR of T3 Promoter + GFP 17:00 PCR of T3 Promoter 20:00 Cut Gel

2009.8.22
12:00 Confirm the PCR product of T3 Promoter

2009.8.23
13:00 Gel cycle of T3 Polymerase and T3 Promoter + GFP 15:00 Double digestion of T3 Polymerase and T3 Promoter + GFP 21:00 Ligation of digested T3 Polymerase and T3 Promoter + GFP 23:00 Transformation of T3 Polymerase and T3 Promoter + GFP

2009.8.25
22:00 Shake the T3 Polymerase and T3 Promoter + GFP

2009.8.26
11:00 Miniprep of the T3 Polymerase and T3 Promoter + GFP 15:00 Double digestion of T3 Polymerase and T3 Promoter + GFP

2009.8.27
0:00 Shake the T3 Polymerase (Strain 3) and T3 Promoter + GFP (Strain 1) 13:00 Miniprep of T3 Polymerase (Strain 3) and T3 Promoter + GFP (Strain 1)

2009.8.28
10:00 Single Point Mutation of T3 Polymerase 20:00 Ligation of Mutated T3 Polymerase 21:00 Transformation of Mutated T3 Polymerase

2009.8.29
12:00 Double digestion of T3 Polymerase (Strain 3) and T3 Promoter + GFP (Strain 1) 16:00 Transformation of CI Promoter Parts

2009.8.30
16:00 Double digestion of T3 Polymerase and T3 Promoter + GFP 20:00 Shanke CI Promoter and 3 Counter 21:00 Cut Gel of T3 Polymerase

2009.9.2
11:30 Gel cycle of T3 Polymerase 14:00 Miniprep of 3 Coumter Plasmid

2009.9.4
12:00 Ligation of T3 Polymerase and 1-2M 16:00 Transformation of T3 Polymerase and 1-2M 20:00 PCR of T3 Polymerase (long primer)

2009.9.6
11:00 Shake the T3 Polymerase + 1-2M 15:00 Cut Gel of PCR Product of T3 Polymerase 15:30 Gel cycle

2009.9.7
11:00 Double digestion of T3 Polymerase PCR Product 11:30 Double digestion of T3 Promoter + GFP

2009.9.11
11:00 Shake 1-1M 12:00 Double digestion of T3 Promoter + GFP (High concerntration)

2009.9.13
12:00 Miniprep of 1-1M 16:00 Gel cycle of Double digestion of T3 Promoter + GFP (High concerntration)

2009.9.14
12:00 Single digestion to ensure the T3 Promoter + GFP Plasmid 16:00 Miniprep of 1-1M

2009.9.16
13:00 Double digestion of 1-1M

2009.9.18
13:00 Double digestion of 1-1M (High Concerntration)

2009.9.20
9:00 Cut Gel of 1-1M 9:15 Gel cycle 11:00 Shake T3 Promoter + GFP

2009.9.21
13:00 Miniprep of T3 Promoter + GFP 16:00 Double digestion of T3 Promoter + GFP

2009.9.22
10:00 Ligation of T3 Polymerase and 1-1M 18:00 Transformation of T3 Polymerase

2009.9.23
20:30 Shake T3 Polymerase +1-1M