Team:Stanford/NotebookPage/22 July 2009

Daily Work
Goals
 * Write-up and establish Wiki goals
 * Begin wiki revamp
 * Learn lab techniques
 * Familiarity w/ protocols for mini-preps and transformations
 * Successful mini-prep concentrations

Project
 * Mini-preps and transformations in the lab

Accomplishments
 * Documented Wiki work
 * Two rounds of mini-preps - both had low results
 * One Round of transformations


 * Made more vBOL images

Problems
 * Low concentrations/ poor results

Alternative Solutions
 * Get more familiar w/ lab techniques
 * Switch away from kit

Protocols
 * [[Media: Miniprep.doc | Plasmid DNA Miniprep Kit Protocol]]
 * [[Media: Transformation.pdf | Transformation protocol

Events

 * 10 am wiki meeting with Bobby, Ming, and Joe
 * See Accomplishments for Designs
 * Goals established and designs made


 * 11:30am Mini-preps with Jerome
 * Low Yields... will redo in the afternoon


 * 2pm Talk Bernard Palsson Clark Center S360
 * Systems Biology


 * 3:15pm Modeling Team Presentation


 * Lab Work w/ Isis
 * Transformations
 * Mini-preps redo

Comments

 * The mini-prep concentrations were very low....

Weekly Goals

 * Work through the code for templates
 * We need to get as many transformations and as much lab work done as possible this week without the genes