Team:Newcastle/Labwork/30 September 2009

=Formal Lab Session - 30th September 2009=

Introduction

 * Since we got the Midi Prep of kinA transformation, we have enough plasmid which are enought for digestion test. We take Chris's advice to use new ladder which would be more accurate in measuring DNA's size.

Digestion
dd H2O                    7ul 10X fast digest buffer    2ul Fast BamHI              0.5ul Fast HindIII            0.5ul Mini Prep DNA            10ul --                           20ul
 * Digestion reaction


 * K1: kinA midi result digested by EcoRI and NheI
 * K1: kinA midi result digested by HindIII
 * Other three digest sample came from Jess's mini prep for sac transformation.

Conclusion

 * Lane 3,4 were K1, K2 digested result. The kinA fragment is 2048bp, so the kinA band should be at the same position with the 6th band of lamda ladder or the 8th band of 1kb ladder. But the gel picture showed that those three bands were not at the same level. Even the ladder are not consist with each other. To confirm our kinA clone result, we need digested pMK-RQ as a standard control.