IBB Pune/8 October 2009

I] Assembly.

Digestions:- ES   Constitutive promoter    Promoter RBS         YcdB

XP    LacO       mRFP              GFP RBS

BKBN A2 from fGFP

ligations Constitutive promoter + LacO Promoter RBS + mRFP Constitutive promoter + RBS YcdB + GFP Promoter RBS + GFP

II] PCR of YcdB with M13f and M13r

III] LuxI (AHL) assay with FeCl3 and hydroxylamine. FAILED :{

IV] Transformation of ligated samples