Team:TorontoMaRSDiscovery/9 October 2009

=October 9, 2009=
 * 1) Miniprepped EncY overnight and digested with E,P
 * 2) Ran digest on a gel
 * 3) *Enc part confirmed
 * 4) Transfected Enc+5 ligations from Sunday/Monday into competent cells from Invitrogen
 * 5) *Used NEB Hight Efficiency Transformation Protocol C2987
 * 6) **did one 10-fold dilution
 * 7) **transformed 5ul of DNA
 * 8) **spread 100ul of cells on plate
 * 9) **shaker/incubator speed 232rpm