Team:Kyoto/CiC/Notebook/1005-1009



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To Do
No experiments were undertaken

To Do
No experiments were undertaken

To Do

 * sig-GFP generator
 * Restriction Enzyme
 * ligation
 * transformation
 * sig-EGFP generator
 * phosphorylation signal sequence primer
 * anneling signal sequence primer
 * ligation
 * transformation

Transformation
Too many colonies were observed that we could find no fignificant differences between the treated culuture the control. (091008)

To Do

 * sig-GFP generator
 * Restriction Enzyme
 * ligation
 * transformation
 * sig-EGFP generator
 * phosphorylation signal sequence primer
 * anneling signal sequence primer
 * ligation
 * transformation

Transformation
Sufficient,but not excessive number of colonies were observed,so we decided to move on to next step.(091010)

To Do

 * No experiments were undertaken

To Do

 * sig-GFP
 * miniprep
 * Restriction Enzyme
 * sig-EGFP(HeLa)
 * miniprep
 * Restriction Enzyme

Results
Plasmid construction is completed!!

To Do

 * Yeast Mitochondria isolation

Results

 * It is too difficult for us to isolate mitochondoria.

To Do

 * No experiments were undertaken