Team:Warsaw/Calendar-Main/6 September 2009

Assembly of endosomal detection operon Marcin Task:1 Construct to transform: Methods: Task 2: Methods: 2 &mu;l purified plasmid DNA product 1 &mu;l RsaI (NEB) 2 &mu;l Buffer Tango (Fermentas) 15 &mu;l MQ water Results:
 * Transformation of chemocompetent E. coli TOP10 strain
 * BBa_K177037 on pSB1A3 plasmid
 * Detailed protocol of transformation is described here
 * Digest isolated plasmid with Bax CDS to verify the correctness of the ligation:
 * Reaction mixture composition:
 * Digest was performed 3 hours

Comments: Bistable switch 1 testing Ania Tasks:    
 * The enzym must have been deactivated due to presence of two forms of the plasmid. If circular plasmid is cut there are only one linear form in the sample. Althought in each case there was two forms - circular and supercoiled.