September/25 September 2009

1.colony check sample no. no. of colony 2-18B         >50 53           >50    X5            >50 X6           >10 X7            2 X8            5

2.min prep result of nano drop. sample no. conc. 11         309 ng/uL 3-1M        14 28         260    29          241    30          219    39          188    46          224    52          218   X4-A         196 X4-B        184

3.transformation sample no. 37

4.digation

K204054

K204055

K204056

↓ 37degree, 3hr Agarose Gel Electro-phoresis ↓ gel cut purification ligation

5.transformation sample no  55, 54

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