Wisconsin-Madison/6 June 2009

June 6, 2009
Other: research

Ex 8: MiniPrep of iGEM plasmid Backbone (I) - (pSB1A3-1)

Boiling Lysis of Scott’s Plasmid (S) - (rMMBRraA) and Hawaii Plasmid (H) - (pRL1383A) (RSF1010 derived plasmids can not be minipreps)

Ran Gels and made freezer stock of DNA



No digest



pRL1383A1-4, Ladder, rMMBRraA 1-2



pSB1A3 1-4, Ladder, rMMBRraA 4,3

Results: Need to redo boiling lysis of Hawii Plasmid - after cleanup, DNA was lost

Need to be more careful or forego clean-up

iGEM - EcoR1 (wrong cut, need to redo, assumed only plasmid, has red gene) Hawaii- EcoR5 rMMBRraA - EcoR1