Team:Gaston Day School/19 June 2009

 High eff. E. coli for (RFP, stop codon, constit. RFP) Thaw E. coli till ice disappears Pippete 50x into tube X3 Add and mix plasmid X3 Suspend the well in 15x TE Add 5x of plasmid to E. coli Place on ice for 30 mins Heat shock at 42C for 30 sec</dt> Ice for 5 mins</dt> Add 950x SOC</dt> Place in 37C for 60 mins</dt> Spin 5 mins</dt> Pipette out SOC</dt> Resuspend in 150X SOC</dt> Spread 50x00x onto plates</dt>