Team:Brown/Notebook Meetings/9-11-09

iGEM Meeting

9/11/09

SFH 218


 * 8:00 AM: Gary reminds us of our responsibility. We need to plan this semester!
 * These next few weeks are the most important


 * Adrian: Deadlines
 * Jamboree is in 7 weeks!
 * Wiki due date: 6 weeks. Adrian recommends we have it done in 5 weeks
 * Presentation practice should be started in 5 weeks
 * Data collection should be heavy these next few weeks
 * Gary:
 * Judging is done by a criteria. Criteria is available to us.
 * Regular meetings: Friday 8AM in SFH 218


 * Team 1 update
 * Did not have any expression on the western blots
 * Made new BL21 competent cells, however no transformations
 * Test competence of cells
 * End of next week goal: protein expression


 * Team 2 update
 * Made all of the constructs needed
 * Agr-GFP ligated
 * Trg-EnvZ
 * Signal Pep-GFP
 * Trouble is transforming into S. epi
 * Looking for new vectors – talking to same guy that sent us PLM6
 * Primers designed for adapting to new vector
 * Will’s computer program worked; designed for Tar
 * He is concerned about mutagenesis – time consuming
 * Submit for synthesis
 * Will will pick 5 candidates


 * Team 3 update
 * We have OmC-RFP and Taz in pNoTat
 * Ashley did stratagene, however her positive control plate did not work
 * We can test with aspartate
 * Will also test ribose binding protein
 * If double transformation does not work, sequential transformation


 * Everybody: come up with a week-by-week goal sheet.


 * The team is meeting next Tuesday at 8AM.