September/5 September 2009

1.Colony check sample No. (no. of colonies) No.5              10 No.9              0 No.18             0 No.20             0 No.22             1 No.23             2 No.24             0 No.25             4 No.27             0

ligation check and transformation again

2. Restriction digest for ligation

K204028

K204029

K204030

K204027

↓ 37°C, 3hr Agarose Gel Electro-phoresis No.27-I,V, No.28-I,V , No.29-I,V , No.30-I,V ,ladder gel cut purification

3.lagation check No.9-A,B,C,D, vector , 18-A,B,C , 26 , vector , 5 , vector , ladder No.18, 5 , 26 : ligaton OK

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