Duke University/17 June 2009

Return

June 17, 2009

 * E: PCR with Bioplastics #4 as template, using Phusion enzyme protocol (shorter denaturation time, lower annealing temperature), Gel to check PCRs

98°C 15s 1x 98°C 1s 50°C 5s 72°C 30s 35x 72°C 2m 4°C infinity 1x


 * R: Correct bands
 * C: Cut out bands


 * E: Measured concentration of DNA fragments 3 and 4
 * R: Concentrations high
 * C: Do assembly PCR