Team:Groningen/Notebook/5 September 2009

GVP Cluster
Plates with colonies were stored in fridge, the indicates a red colony. Plates without colonies were left on the bench.

Metal Accumulation
Colony PCR  Colony PCR of SmtA colony #1 using Biobrick VF2/VR primers (lane 1) and RBSforward and SmtAreverse primers (lane 2). Probably a different RBS primer was used, explaining the lack of product. SmtA (including RBS) is 678 bp, PCR product using VF2/VR primers should yield a band of 989 bp. Left and right are both the same 1% agarose (1xTBE) gel on different time points.  Restriction analysis Restriction analysis of of SmtA colony #1 using PstI and XbaI. Expected fragment was ~700, plasmid was pSB1AC3 about 3000 bp. 1% agarose gel (1xTBE)