Illinois/6 July 2009

July 6
sRNA Library

We performed another PCR of all of the new sRNA's, and we ran a gel with the 5 sRNA's from last week.



Lane 1   100bp ladder 2   ftsZ 3   ftsZ* 4   oppA 5   oppA* 6   dppa 7   dppa* 8   hns 9   galK 10  sodB



Lane 1   100bp ladder 2   dicF 3   dicF* 4   gcvB 5   gcvB* 6   rseX 7   spot42 8   ryhB 9   dsrA

The genes that have an asterisk are the samples from July 2st, and none of them seemed to show bands. SodB, spot42, and dsrA also didn't work.

We ran a digestion with the PCR products that did work, however we did not do the PCR purification.

Modeling

Transformations of GFP and the reference standard promoter were successful. We successfully miniprepped the DNA for both Biobricks.

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