Template:Team:KULeuven/18 September 2009/VanillinProduction


 * Miniprep EFT


 * Restriction digest EFT with X/P
 * Gel electrophoresis EFT for 1,5 hour, because the difference between insert (2600bp) and vector (3100bp) is small.
 * EFT is present in all 4 samples
 * Gel extraction of EFT
 * Ligation of the gel extract and the promotor, and ligation of the non-gel extract and the promotor.
 * Transfered 6 colonies of PAMS to a new plate and on liquid culture.