Team:Chiba/Notebook/Calendar/23 September 2009



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(22_September_2009 <|>24_September_2009)

E. coli Painting (2)
We draw various pictures with ink brush and throwaway chopsticks.

Yesterday's operation is here.

To judge character of LuxR mutants (2)-2
Yesterday's operation is here.

10:30
 * Today's operation

We transplanted E.coli, which has been cultured on NC filter, to solid medium which contains each concentration of AHL.

AHL concentration is : 0, 1, 10, 100, and 1000 nM

We decided this time is T=0 and observed condition of fluorescence every 30 min.


 * Mutants' Location

Pictures are here.

Examine limit of AHL generation(1)-2
Yesterday's operation is here.

11:00
 * Today's operation

We did main culture.

22:00

We measured OD.

OD600=1.98

Then, we spun down this culture solution and add 12.5 mL of flesh LB-Amp.

22:10

We add 12.5 &mu;L of IPTG to the culture solution and started culture at 37 degrees Celsius.

Transformation(2)-1
plux-GFP(Cm, p15A)
 * Plasmid

JW1262(pCIA3-LuxR(Amp))
 * Competent Cells

JW1262(Null)

21:35

We transplanted these bacteria each plates.

pCIA3-LuxR and plux-GFP --> LB-Amp, Cm plate

plux-GFP only --> LB-Cm plate

21:50-

We cultured these plates.