Team:Kyoto/CiC/Notebook/1012-1016



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To Do
No experiments were undertaken

To Do

 * sequence
 * sig-GFP generator
 * sig-EGFP generator(HeLa)

Results

 * We assured that the plasmid include the correct sequence by analyzing it with sequencer.(091015)

To Do
No experiments were undertaken

To Do

 * Experiments to approach subgoalA
 * Making of liposome by natural swelling method and extruder

To Do

 * Experiments to approach subgoalA
 * Making of liposome by natural swelling method and extruder
 * expression in vitro HIV-TAT-(LALAAAA)3-generator
 * add liposome to HeLa cells

Results
No NBD-PE signal was observed in HeLa cells. This might be because we did not collect the right fraction after density gradient centrifugation and thus lost the liposomes.

To Do

 * Experiments to approach subgoalA
 * add liposome including synthesized HIV-TAT-(LALAAAA)3 peptides to HeLa cells

Results
No NBD-PE signal was observed in HeLa cells.

To Do

 * observation by microscope

Results
We could't confirm that liposome was not taken by HeLa cells.