Team:Warsaw/Calendar-Main/17 October 2009

Creation of the cro-box testing devices Kamil

Tasks:  Colonies selection R0010 brick isolation 

Methods:  Appropriate colonies carrying a cro-box+reporter device were selected (5 for each variant) and transferred to a new plate. Liquid cultures were established along the way. (in LB supplemented with ampicillin). Appropriate colonies carrying the I14018 brick were selected and transferred to a new plate. Liquid cultures were established along the way.(in non-supplemented LB medium). The R0010 birck was isolated 4 times from 3&mu;l of overnight culture using the Plasmid Mini kit (A&A Biotechnology). The transfer plate and the liquid cultures were incubated in 37&deg; overnight. 