Wisconsin-Madison/27 May 2009

May 27, 2009
Ex 4: Keasling plasmid arrived on plates made 3 liquid cultures:

1.	pMevT: DH10B: Cm resistance

2.	pMBI: DH10B: Tet resistance

3.	pMBIS: DH10B: Tet resistance

Ex 3: Competent cell preparation results on amp plates: 50mL stock

Stock: 150 colonies, 100x: 8 colonies, 10,000x: ---



- Dr. Keasling's materials received, pMBI (Tc), pMBIS (Tc), pMevT (Cm) - NP

- inoculated liquid cultures of pMBI, pMBIS, and pMevT in LB with appropriate antibiotics