Team:TUDelft/5 August 2009

=5 August 2009=

Sriram
Today I did the restiction along with Daniel. After lot of calculation and discussion we did it successfully. Then I prepared a gel and loaded all the restricted samples in the gel and it could be seen in below image:



Then we decided to continue the assembly tomorrow. Daniel gave me the instruction and he decided to check with how to do the GFP expression experiments.

Tim Weenink




Orr
Prepared 1 liter of LB medium, and helped Calin with the Conjugation procedure.

Calin
Made test plate for Trimpethoprim.

Ran conjugation test with R751 (TRI) as donor and DH5 &alpha; cells with GFP plasmid and AMP as recipients.

Ran three conjugations in parallel, with a conjugation time of 1 hour.

Starting OD for recipients 0.502, for donors 0.542.

Made a total of 40 plates: