Team:Chiba/Notebook/Calendar/13 September 2009



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(12_September_2009 <|>14_September_2009)

Check of Sender's AHL generation (3)
7:10-
 * Main culture

12:20
 * Measuring OD

OD600=1.98

12:40
 * Wash

First, we dispensed culture solution about 12.5 mL to each 10 centrifuging tubes.

12:45

Next, we centrifuged samples 3 times(rpm=3000, 5 min, 20 degrees Celsius).

13:50
 * Centrifugation

We added more 12.5 mL of liquid medium and 12.5 &mu;L of 0.1 M IPTG to each tubes.

After that, we centrifuged one of samples, and cultured the others at 30 degrees Celsius.

And then, we picked 10 mL of supernatant solution and saved it.

-- We picked supernatant solution every 15 min.--

16:15

We prepared mixture and made solidified media using this.


 * mixture : 10 mL LB-agar solidified medium and 10 mL supernatant solution

17:20

We transferred colonies onto NC filters and cultured it at 37 degrees Celsius.

18:05

We put NC filters on plates which has been prepared a little while ago.

After that we started observation of GFP fluorescence.

Pictures are here.

21:05
(4)-(10) were became luminescent.

21:35
(3) has became luminescent.

Check of Sender's AHL generation (1)
This Sample use the AHL, making by the Sender's culture without IPTG.