EPF-Lausanne/14 October 2009

  

14 October 2009

Wet Lab
Transformation of LOVTAP in iGEM plasmid worked --> Check if the insert is correct with Colony PCR & agarose gel

Also: our negative control worked (no clones grew)

Dh5-alpha RO2.4+BB1 n°3

We redid the initial experiment with these cells: incubated them for about 2h30 in 4 different conditions:

- +light+IPTG-Trp

- -light+IPTG-Trp

- -light-IPTG+Trp

- -light-IPTG-Trp

The results are the following:



RO1.1 + BB1 JRG1046 cells

Did an experiment on the new double-transformants for RO1 in the TrpR-mutated strains: 0.5 mL of overnight culture in 3.5 mL of fresh LB. Since we had 4 different clones, for 3 of them we did only the +light+IPTG and -light+IPTG conditions, and for the 4th one we added the -light-IPTG+/-Trp conditions. The cells were exposed to conditions during about 2h before we took the measurements of OD and fluorescence with the plate reader:





People in the lab
Gabriela, Tú

 