IBB Pune/21 June 2009

I) Transformation with PCR product, dilute DNA 1:10 and use 4ul.

NO Transformants :-(

Why?
 * Dilution?
 * Linear DNA, try ligation before transformation.
 * Kan conc too high?

II) Ligation test. RE digest of pBR322 and pGFP, gel and ligation.