ULB/14 August 2009



 iGEM Team:ULB-Brussels Wiki  

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Université Libre de Bruxelles

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 Home Team Project</a></li> Parts</a></li> Safety</a></li> Notebook</a></li> Sponsors</a></li> </ul> <ul>

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A glycerol is ready for bacteria having integrated the RBS.

Plasmids containing the RBS have been purified using the purification site (2x 1500µl of culture). 7µl of each purification were digested with 1µl of XbaI, PstI and 1µl of tampon H.

An electrophoresis on gel confirmed the presence of the plasmids in each tube. Parasites on the first tube are probably due to an incomplete digestion.



The quantity of purified plasmids was evaluated using a photometry analysis on a 100x dilution.

All plasmids show a concentration close to 50ng and a very acceptable purity.

hfsG and hfsH have been re-suspended in water (50µl) and then diluted 20 times.

The ligation of the promoter and the RBS in the destination plasmid have been stopped by freezing those at 80°C during 20 min.

Bacteria have been transformed with hfsG, hfsH (to keep a stock of plasmids) and our 2 ligations (in order to check wether it did work or not).