Indiana/6 July 2009

Grow liquid culture to prepare for mini prep

Preparation of Media:

The modified plasmid is kan resistant and the parts pulled from the tool kit are amp resistant, so both media were made.

Kan media:

50 mL LB

50 uL KAN

Amp media:

50 mL LB

50 uL amp

add 3 mL of media to a tube

transfer 1 colony into each tube

shake @ 37 C o/n