Team:Kyoto/CiC/Notebook/0928-1002



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To Do

 * sig-EGFP generator(HeLa)
 * Restriction Enzyme Digestion(Hind3)

Results
They were unexpectedly digested by Hind3,the Restriction Enzyme which would not work if the signal sequence were inserted in the vector correctly.

To Do

 * sig-GFP generator
 * PCR(the second PCR)
 * sig-EGFP generator(HeLa)
 * phosphorylation signal sequence primer
 * annealing signal sequence primer
 * Restriction Enzyme Digestion(EcoR1 + Xho1)
 * ligation
 * transformation

Results
No colony was observed (090930)

To Do

 * sig-GFP generator
 * miniprep I712074(T7 promoter)
 * Restriction Enzyme Digestion
 * ligation
 * transformation
 * sig-EGFP generator(HeLa)
 * ligation
 * transformation

Transformation
Too many colonies were observed that we could find no fignificant differences between the treated cluture the control. (091001)

To Do
No experiments were undertaken

To Do
No experiments were undertaken

To Do
No experiments were undertaken

To Do
No experiments were undertaken

To Do
No experiments were undertaken