August/19 August 2009

1.before Min prep Checked the cell cultures transformed and plated out yesterday (8/10) for colony formation and made an approximate count of the number of colonies.

(sample no.) (no. of colonies) ③                    10     (Amp) ④                    50~   (Amp) ⑤                     1     (Kan) ⑥                   ~10    (Amp) 4 colony (each ③,④,⑥) inoculate to iquid medium(5mL + Amp 5uL)

2. 0.1M MgCl2, 0.1M CaCl2 buffer

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