Lab July 3 2009

July 3, 2009

500 mL of 100 mM EDTA at pH 7.0

14.61 g EDTA in ~250mL water

pH to 7.0 and top up to 500 mL

1L TE buffer @ 10mM Tris pH 8.0 and 1mM EDTA

10 mL Tris 8.0 stock, 10 mL EDTA stock, top up to 1L

1L P1 buffer @ 50 mM Tris pH 8.0 and 10 mM EDTA

50 mL Tris 8.0 stock

100 mL 100 mM EDTA stock

(RNaseA to be added at usage time)

1L PE

10mM Tris-HCL pH 7.5, 80% ethanol

842 mL 95% ethanol (equiv to 800 ml of 100% ethanol)

10ml Tris 7.5 1M stock

top up to 1L with distilled water

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