Team:Washington/Future

=Overview=
 * Target Construct
 * 1) Attempt to add additional proteins into the vector and test for functionality
 * 2) Vary linker lengths
 * 3) Make a simpler version for trouble shooting the secretion system: [6x-His]-[NheI]-[prtB]
 * 4) Transfer to a vector with the same origin and resistance as described in the original secretion system (pBR322+Carb).
 * 5) Add a lacI into the expression and target vectors so repression is hard-coded into the vector and expression can be induced regardless of the cell line.


 * Secretion System
 * 1) Transfer to a Chlor resistance, p15A origin vector as used in the original papers
 * 2) Add original upstream DNA (50bp) before the native RBS to ensure proper function
 * 3) Add an arabinose inducible promoter for better control over secretion system activation
 * 4) Combine with target vector so entire secretion system is contained in one plasmid.


 * Display System
 * 1) Construct a new modular display system:  CDS
 * 2) Use computational protein design to create a monomeric protein that binds tightly to biotin:  FoldIt

Continue to Accomplishments and & Submitted BioBricks