August/11 October 2009

1.colony check sample       no. of colony chiba 2            + chiba 3            ++ chiba 4            + 31                  +++  58                    5  62                    3   73                     0  76                    >20  77                     7    78                    >30

2. min prep sample               conc. 1-23L                  20 ng/uL 1-23L                  113 22                       196   47                        167   40                        130   25                        164   31                        31      X4                       265 X4                      269 59                      142   70                       325   72                       281

3.diagtion

K204079

K204080

K204081

K204082

K204083

K204084

K204067

K204068

K204071

K204075

↓ 37 degree, 2hr gel cut & purification ligation

4. min prep sample               conc. plac CFP              314 ng/uL ptet CFP              179 plac mOrange     45 ptet mOrange     170

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