Team:Paris/23 July 2009

NoteBook
July 23th

 

Brain work

 * Modelling:
 * Proteic binding force estimation for Tol/Pal system:
 * use a simple estimation based on the existing values.(E_Prot=10-4O kJ/Mol*N_Prot)
 * use a simple estimation based on the existing values of the OmpA familly.
 * Docking values given by our own work on TolA/Pal.(aborted)
 * Osmotic pressure estimation
 * A mean on the wall cycle of the bacteria: 75 lb (bionumber estimation)
 * Osmolarity of the periplasm:~300 mOsm
 * Total model:
 * Direct and simple shemtaics of the Model was designed: complet scheme begining of the choices for numerical values.

Sec-Tat Pathway:
 * reading of 2 articles on tranlocation cinetics via Sec & Tat pathway
 * the translocation rate of amino acids per time unit is constant on the Sec pathway

Ideas :
 * Use a model of active diffusion across membrane
 * Think about an evenemential simulation

 

Lab work

 * DH5 alpha competent bacteria by CaCl2 protocol

 

To do list
Find the constrcution that are necessary for the fec system that Braun can't provide us with.

Modelling
 * Get data on osmotic pressure.
 * Find information on active diffusion across membranes.
 * Find kinetics datas for complexation of tol-pal interaction as well as the translocation via tat and Sec export system.
 * Get a better understanding of PMF.


 * Test genomic DNA purification of S2
 * More competent bacteria