Template:Team:KULeuven/14 September 2009/VanillinProduction


 * Miniprep of COMT
 * Restriction with S/P, SAMS2 P (was already cut with E/X) and EF3 with E/S
 * Another attempt at electroporating EFT using EFT ligation from last week
 * Gel electroforesis of 5 &mu;l from the restriction sample (30 &mu;l)
 * Nothing in EF lane (no DNA???)
 * Promotor showed again two results of 900bp and 2k bp (wut is going on?)
 * An attempt was made to seperate the restriction enzymes by PCR purification on the remaining restriction sample, however nanodrop showed very confusing results, conclusion: let's not try this again. Next time heat inactivation of restriction enzymes to make sure they are inactive.
 * Made mother plate of SAMS