Team:Wisconsin-Madison/Cloning Protocols

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Overview of Cloning Protocol

Insert and Vector Insert Only Insert and Vector
 * Solid Culture
 * Liquid Culture
 * Plasmid Isolation
 * Restriction Enzyme Mapping for Verification
 * PCR to add BioBrick Restriction Cut Sites (Primer Design)
 * PCR Plasmid Cleanup
 * Concentration Verification
 * Digestion
 * Digestion Clean Up
 * Concentration Verification
 * Ligation
 * Transformation
 * Colony PCR Screening

** Procedures for these can be found in Protocol Section! ** Back to Protocols