September/16 September 2009

1. colony check

sample No.  no.of colony 17           0      45            0      46            0      48            0     1-1D          +++ 2-18F        +++ 19          +++     1-8I          +++ 1-2M         +++ 17           3      38            0      45            0      46            0      20           >50      27           >10      28           >50      29            0      30           >10      35            3 for DNA modification 1-1K         +++ 41          +++      42           +++     1-16I         +++ 18          +++

2.digation for ligation

K204049

49-I,V, 1-14A , 2-24I , 14A , 48 , 47B 14, 47 : OK

3.Mini prep

result of nano drop sample no. conc. 1-1K m+      71.8 ng/uL 1-4K         21.6 ng/uL 1-16C        27.8 ng/uL 1-16I m+     46.1 ng/uL 18 m+       49.3 ng/uL 41 m+       58.4 ng/uL 42 m+       83.2 ng/uL

4.transfromation No. 45, 46 , 49

5. ligation quick check

UP  : 17-A,B,C,D, 20A,B , 27-A,B , 1-6I , ladder , 35-A,B,C , vector DOWN : 29-A,B, 2-18H , 30-A,B , 2-18F , laldder , 48-A,B , vector , 31-A,B

6.Mini prep result of nano drop sample No. conc. 1-8I          11.2 ng/uL 1-2M          12.7 ng/uL 1-1D          10.2 ng/uL 2-18F         13.6 ng/uL

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