Team:Paris/ProtocolsMB

iGEM > Paris >  Protocols >  Molecular Biology

PCR with Quick load Taq2x Master Mix
 position: absolute; height: 23px; width: 30px; top: 0px; left: 40px; margin-top:10px; padding-top: 7px; background: url(http://2009.igem.org/wiki/images/1/1b/Left_menu_pari.png); z-index:4; }
 * 1) left-side {

height: 25px; width: 560px; position: absolute; top: 0px; left: 60px; margin-top:10px; padding-top: 5px; background: #dadada; z-index:5; }
 * 1) middle-side {

position: absolute; height: 23px; width: 30px; margin-top:10px; padding-top: 7px; top: 0px; left: 600px; background: url(http://2009.igem.org/wiki/images/4/40/Right_menu_paris.png); z-index:4; }
 * 1) right-side {

a.menu_sub { padding-left: 7px; padding-right: 7px; }

a.menu_sub_active { padding-left: 7px; padding-right: 7px; color:#b0310e; font-weight:bold; }  Main |  Microscope Protocols|  Adapted Protocols|  Culture Protocols|  Molecular biology


 * Mix for 50µl final:
 * 14µL H2O
 * 25µL Taq Quick load Master Mix 2X
 * 5µL Oligo Fw (0,2µM)
 * 5µL Oligo Rw (0,2µM)
 * 1µL Matrice (DNA)