Team:PKU Beijing/Notebook/Assembly/Rencheng Gao

Notebook > Assembly > Rencheng Gao's Note

2009.10.1
10:00 Double-enzyme digestion:

14:00 Gel purification

16:00 Ligation: Vector: Bistable Insert: T7-CI(1H, 2M, 2I, 2G, 2K, 1J) Ligase: 1μl Buffer: 1μl

18:00 Transformation

2009.10.2
10:00 Bacteria culture: Bistable-T7p-CI(1H, 2M, 2I, 2G, 5N, 1J)

24:00 Mini-prep: Bistable-T7p-CI(1H, 2M, 2I, 2G, 2K, 1J)

2009.10.3
09:00 Double-enzyme digestion: Bistable-T7p-CI(1H, 2M, 2I, 2G, 2K, 1J)

16:00 Ligation: Vector: Bistable on 1O and on 2K Insert: T7p-CI(1H, 2M, 2I, 2G, 2K, 1J)

20:00 Transformation

2009.10.4
09:00 Bacteria culture: 1O-Bistable-T7p-CI-2K, 1O-Bistable-T7p-CI-2G, 1O-Bistable-T7p-CI-2M, 12K-Bistable-T7p-CI-2I, 12K-Bistable-T7p-CI-1H, 12K-Bistable-T7p-CI-1J