August/6 October 2009

1. coloony check sample no. no. o f colony 59                       0   61                        0   59                        1   61                        4   64                       ++   65                        6   X5                        10 X6                       + X7                       10 X8                       10 1-24E                   50

2. min prep sample     conc. 58           174 ng/uL 60           220   62            267   63            237    64            151   65            75   67            252   68            233   X3           236 X4          193

3.transformation sample no. 11, 20 , 21 , 2-6O , 1-15J , 1-15L

4.digation K204052

K204067

K204067

K204068

↓ 37°C, 2hr gel cut & purification ligation O/N

5.prepared LA pate

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