Team:Chiba/Notebook/Calendar/2 September 2009

>Go to the Notebook page

(Lab Work Start! |>3_September_2009)



Making Fluorescent Transfer Curve for the Practice of Lab Work (Day 1)

 * prior culture

medium:liquid culture medium antibiotic:Amp

We took this medium to centrifuging tube 50 mL and added antibiotic(Cm) 50 &mu;L. Then we transferred the medium to test tube 2 mL and added glycerol stock(BW25113, &Delta;Tdk,pCIA3-LuxR,pLux-GFP) and then started shake culture at 37 degrees Celsius. (21:40-)