Team:Virginia/10 June 2009

Overnight Results

 * Only saw growth in pSB1C3 broth
 * Have colonies on A09-1(2) plate

Today

 * Made 5ml overnight broths from 3 colonies on A09-1(2)
 * Miniprepped pSB1C3
 * 18.4 ng/ul
 * Re-transformed vector DNA in DB3.1
 * included control transformation in DH5a, should yield no colonies
 * Prepared -20 glycerol stocks of knockouts JW3469 and JW3470.