Team:Wash U/Calendar-Home/11 June 2009

Characterization of System
(1) -puc Promoter via GFP (inserted with primers) -want high expression (denotes high conc. of light sensor) (2) -control: puc-OmpR-Term + OmpC-GFP (test native phosphorylation of OmpR) (3) –measurement: puc-Cph8-OmpR-Term + OmpC-GFP (test synthetic phos. of OmpR) -combination of puc and ompc characterization will give expression rates of puc, ompR, and how they integrate -compare natural R. sph. absorbance to new altered strain (LH2 levels) -extent and development rate -strength of native RBS -switch-on, switch-off behavior over time (step inputs of light) -measured in both GFP fluorescence setup and LH2 absorbance -degradation of LH2?

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