August/10 August 2009

1.Min prep Result of Nanodrop concentration check

(sample No.) (concentration) 1-6O           35.2 ng/uL 1-18L          41.8 ng/uL 1-10K          39.1 ng/uL

2.make competent cells(with CaCl2)

3.digestion and ligation digestion with restriction enzyme

↓ vector1 insert1 vector2 insert2 ladder failure

digestion with restriction enzyme insert : E+S vector : E+x

4.Transformation to get new plasmid 1-23L A 1-18C A  1-20F K  1-12H K  1-6I  A  2-6P  A  1-15N A used '8/10 competent cell

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