Template:Team:KULeuven/5 August 2009/VanillinProduction

Miniprep of pairs:
 * RBS + sam5 conc: 101,3 ng/ul
 * RBS + sam8 conc: 95.7 ng/ul
 * sam8 conc: 81,8 ng/ul
 * COMT, conc: 122,9 ng/ul

Restriction digestion A1: sam8, EcoRI en SpeI A2: sam5, EcoRI en XbaI B1: RBS, EcoRI en SpeI B2: COMT, EcoRI en XbaI C1: fcs, EcoRI en SpeI C2: ech, EcoRI en XbaI

The mixture was incubated in 37°C for 1h30...

...followed by gel electrophoresis of the digested parts