Team:Chiba/Project/for painting

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To draw more better picture

 * we screened the "agar media condition" (the thickness & the agar conc.) to enlarge difference in the delay of the LuxR mutant.


 * Finding more useful condition of medium to demonstrate

We researched the best condition of medium to display our demonstration more clearly.

The protocol of this experiment is here.

First, we did noted above experiment using GFPuv as reporter. Also we did similar experiment using sfGFP, mRFP, mCherry, and mOrange to judge our mutant LuxR.
 * About the difference of responce of each reporters

As a result, we found that GFPuv is better reporter for our demonstration.

The protocol of this experiment is Distinction of fluorescence protein expression between reporters