Wisconsin-Madison/23 June 2009

June 23, 2009
Ex 10:	 Set up fish tank with bubbler of CO2

12 hour timer – or else cyanos will photo-bleach with too much light

Ex: Miniprep of

1.	gsSDMT

2.	(pSBA1) ProU BB

3.	(pSBA1) ProU, GFP BB 	Digestion:



Digested(proUBB, GFP+ProUBB, proU, GFP+ProU) Ladder undigested (proU, GFP+ProU)

NO IDEA WHAT THIS MIGHT MEAN!

ProU, GFP BB

Might get better readings with smaller load – could be super concentrated

Ex 18: pTaq induction

Ex 17: GFP Regulation by ProU Promoter

Test 1:

Test of GFP and Pro Biobrick

'''Conc. / Culture (uL) / 5M NaCl (uL)'''

0.0 / 200 / -

0.2 / 192 / 8

0.4 / 184 / 16

0.6 / 176 / 24

0.8 / 168 / 32

1.0 / 160 / 40

Well Assignment:

'''Conc. /  Wells'''

0.0 / D5 / E8 / C6 / F9

0.2 / E5 / D8 / F6 / C5

0.4 / D6 / F4 / C8 / F8

0.6 / E9 / D5 / C7 / F7

0.8 / D9 / E4 / D7 / C4

1.0 / C9 / F5 / E6 / E8

All other wells were filled with MilliQ water

Ex 10: Cyanobacteria

Inoculated two cultures of PCC 7942 to test the new aeration system (one normal flow, one restricted flow) - NP

-tested freezer stock of PCC 7942 by plating on BG11 (used entire 1mL stock) check ~June 30 for results (plates dehydrated so unsuccessful) – NP