Wisconsin-Madison/24 August 2009

August 24, 2009
digest of possible pBAD24 clone with AflIII should give fragments of size 1500, 1680, 3470

2uL DNA

1uL AflIII

13uL Water

1.8uL NEB Buffer III

.18uL BSA

two hour digest

Inoculated possible ProU/RFP in salt solutions, plated on NaCl amp plate.