Virginia Commonwealth/30 June 2009

Results
Maria and Afton
 * E. coli cells Deb 3.1 with the plasmid backbone psB3k3 p1010 (death gene) had no visible colonies
 * Negative (-) Control of the Deb 3.1 on Kan has small colonies visible on it
 * other Negative (-) and positive (+) controls on LB plates had a lot of colonies.
 * Possible reasons for lack of growth on Kan plate
 * Kan plates were incubated at a 47 degrees Celsius (not 37) which may have caused a problem
 * Possible mislabeling of plates (negative kan control and plasmid plate)
 * Waiting too long to add the LB broth after zapping the cells

Tasks
Maria and Afton
 * Retransform cells

Wetlab
Maria and Afton Trentay 21:26, 30 June 2009 (UTC)
 * Cells were retransformed with DNA from BioBrick stock and e. coli cells DEB 3.1
 * The controls for the experiment are:
 * E. Coli Cells on LB (+)
 * E. Coli Cells on LB with KAN (-)
 * E. Coli Cells Zapped on LB (+)
 * Care was taken to keep everything chilled and to immediately add LB broth after zapping cells.
 * For the e. coli that was not zapped, 35 microliters was taken directly from the cell stock and placed on a Kan plate, and 35 microliters was placed on an LB plate
 * Cells will be incubated overnight