Team:Paris/1 September 2009

NoteBook
September 1st

 

Lab work
Verif purif

Products of: PCR:
 * FecI
 * FecR (A) &(B) (B) Failed
 * Tg3p Failed

digestion


 * Plac rbs tetR
 * TE3
 * RBS
 * TER

purified digestion
 * pTet
 * Cly A (N term & C term)

purification on gel

Products:
 * FecI
 * ClyA N term
 * FecR (A)
 * RBS
 * TER

ligation with soufifi

Products:
 * pFec (A) / P17
 * TER/Plac rbs tetR
 * RBS/YFP
 * TE3/PSB1A3
 * RFP/pTET
 * RFP/TER