Team:Wisconsin-Madison/Ligation

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Ligation

Concentration
 * 1 backbone : 5 insert
 * insert: [100 x (insert size bp/backbone size bp) x 5 / concentration(ng)] μL

Thermocycler
 * users: Dan A: Lig
 * 2 hours 16 C or overnight

Materials
 * 1μL T4 ligase
 * 1 μL T4 ligase buffer
 * backbone + insert =< 8μL (remainder H20)

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