Team:Chiba/Project/medium

First, we made 4 styles of medium.

We put Nitro Cellulose filter on these 4 styles of medium and cultured 13 of mutants LuxR each 3 colonies.

After cultivation, the mudium gel with nitro cellulose filter was quarried and layered on 2000 nM AHL containing media with same thickness. Thereafter We obtained the fluorescence pictures.


 * Results

The No.4 medium afforded the most delayed time among the LuxR mutants.

Namely, the thin medium of agar was better for our animation than the thick medium.