Team:Paris/27 July 2009

NoteBook
July 27th

 

Lab work

 * Media: 15 LB agarosis ampiciline dishes were made.
 * PCR for oligo P1-P2 (tolR); P3-P4 (tolA); P5-P6 (tatABC); P7-P8 (clyA fusion).
 * Matrice is Genomic DNA of [S2] K12 1655 &Delta;recA::Kan. PCR Quick load protocol
 * PCR launched with a Tm at 60°C (programme quick load).
 * Buffer for Competent bacteria by RbCl
 * Buffer I (250ml)
 * Buffer II (125ml)


 * DH5 alpha competent bacteria by RbCl

 

To do list
migration of the PCR product on a BET gel.