Uppsala-Sweden/27 July 2009

2009-07-27 11:15,OD measurement of Butanol at 750nm,

pirA PCR was conducted, using 1. Primer:pirA forward pirA reverse pirB forward at three different template concentration,

2. Primer:pirA reverse pirB forward at three different template concentration, Control pirB

PCR PROGRAM denaturing temperature 98 denaturing time: 30s cycle: 5 denaturing temperature 98 denaturing time: 10s anealing temperature: 63, time 30s extension time:32s extension temperature 72 cycle: 30 denaturing temperature 98 denaturing time: 10s annealing temperature: 72,time 30s extension time:32s extension temperature 72 hold 5 min

-- Emily