Illinois/16 July 2009

July 16
Modeling

We set up digestion reactions for our plasmids, GFP, and promoters. The promoters required digestion with two noncompatible cutters (EcoRI and SpeI), so we are digesting first with EcoRI. We did not have enough DNA to run digestion reactions for the two arabinose promoters, however, so we may have to redo those transformations and minipreps.

After three and a half hours, we ran a gel on our digestions to gauge how much longer digestion should continue. We ended up running all our digestions for eight hours, then we heat-inactivated the restriction enzymes and performed PCR cleanups on the DNA.

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