Virginia Commonwealth/23 June 2009

Results

 * n/a

Tasks

 * Perform Digestion
 * Run a Gel Electrophoresis on Digests
 * Perform Ligation

Wetlab

 * Digested these parts: BBa_J23102, pSB3K3 (1), E0240 (1)
 * Gel Electrophoresis
 * Steps for making the gel:
 * 0.50 g Agarose
 * 50 mL 1x TAE Buffer
 * 0.5 uL cyber safe stain.
 * Heat in microwave. Pour in plate. Rake out bubbles and place well mold.

Gel Results
 * The ladders: 2uL were used with 4 uL of water
 * The DNA samples: 4 uL of dye were used with 20uL of sample


 * The digestion on the backbone did not work. The DNA had degraded, indicated by a sharp decrease in the concentration of the DNA sample that it was taken from.  The cell stock of the biobrick is being grown overnight so that more plasmid DNA can be purified.
 * Ligation
 * Since the backbone piece was not present on the gel electrophoresis, the ligation was frozen and not transformed. Another ligation will be done tomorrow morning as well as purification of the backbone.