Virginia Commonwealth/2 July 2009

Results
Afton and Maria Trentay 18:48, 2 July 2009 (UTC)
 * All replated (-) controls grew on both KAN plates (lots of little colonies)
 * Troubleshooting this problem will be abandoned and new cells will be grown up and tested to ensure the protocols are being done correctly
 * The one visible colony on the psB3k3 p1010 backbone with DB 3.1 have grown. It's still just one colony but it is much bigger.
 * Overnight culture was successful

Kevin and Adam
 * parts J23101 and pSB3K3 showed no growth
 * (+) controls for NEB10B and DB3.1 showed lawn growth
 * (-) controls for NEB10B and DB3.1 showed no growth
 * part J23119 showed < 10 colonies
 * part E0840 showed hundreds of colonies

Tasks
Afton and Maria Trentay 18:48, 2 July 2009 (UTC)
 * MiniPrep overnight culture
 * Make stocks from overnight culture to freeze
 * Begin planning out/documenting Project A in Microsoft Word.
 * the goal is to keep a running draft

Wetlab
Afton and Maria Trentay 18:48, 2 July 2009 (UTC)
 * MiniPrep was done
 * Cryogenic storage of DB 3.1 with backbone was frozen in the -80 degree Celsius freezer

Kevin and Adam Bussingkm 23:28, 2 July 2009 (UTC)
 * parts J23119 and E0840 were cultivated overnight from plate growths
 * parts J23101 and pSB3K3 were cultivated overnight from cryogenic cell stock