Team:BIOTEC Dresden/Notebook2-3

14th September’09:

1. Minipreps of GB05MN-Fosmid-BsdR pSC101-SpectR has been done and concentration is measured.

2. A PCR for KanR2 was done using Kan-Fwd and Kan-Rev primers.

3. Colonies are saved from both plates.

4. A sequencing reaction has been set up for the positive clones of GB05 RhaFlpCmR with primerF and primerR primers using Taq polymerase.

5. A enzyme digest for GB05MN FosBsdR-pSC101SpectR has been set.

17th September’09:

1. A test digest run is performed with the minipreps from fosmids using HindIII enzyme.

18th September’09:

1. Minipreps of pF3-ZeoR-F3-RFP and TT-FRT-GFP-BsdR has been.