Wisconsin-Madison/17 October 2009

October 17, 2009
'''Restriction Digestion (with NotI) to confirm our Biobricks:

pSB1A2+GSMT

pSB1A2+Gssdmt

pSB1A2+nudF

pSB1A2+metK

pSB1A2+ProU

2 fragments anticipated (Insertion+Backbone)

'''50uL / DNA / Buffer / H2O / Enzyme

ProU / 40 / 5 / 4 / EcoR I 1 SpeI 1.5

ECFP / 40 / 5 / 4 / EcoR I 1 XabI 1.5

GYFP / 40 / 5 / 4 / EcoR I 1 XabI 1.5

SCFP / 40 / 5 / 4 / EcoR I 1 XabI 1.5

PCR clean-up

Run gel

Ligation