Making delayed-LuxR mutants

Experiments

 * 1) luxR gene was mutated by error-prone PCR using Mn2+(Joyce, 1996) and cloned it into a pUC vector.
 * 2) This library was transformed into XL10-Gold, and the plasmid was mini-prepped to get an plasmid library (pUC-[luxR]).
 * 3) The plasmid library was transformed into JW1226 harboring pAC-plux-gfp, which will glow green when an active LuxR was present.
 * 4) We screened for the fast/dull mutant of LuxR, and collected the clone's plasmid.