Team:Kyoto/GSDD/Notebook/0831-0904



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To Do

 * Miniprep p2-1-1, p2-1-2, p2-4-2
 * Transformation p2-4-1(retry)
 * MPR

Miniprep

 * p2-1-1, p2-1-2, p2-4-2 was minipreped.

Transformation

 * p2-4-1 was transformed, and colony was observed.

MPR

 * xxx was MPRed.


 * Bands were observed on samples with Fw2.
 * Bands were longer on samples with Rev1 than with Rev2.

To Do

 * Electrophoresis of MPR produnts on 31 Aug.
 * Miniprep

Electrophoresis
(See lab-note on 31 Aug.)

To Do

 * Miniprep of p2-4-1
 * PCR of (7), (8), (10)
 * PCR of (7), (10) (retry)

Miniprep

 * p2-4-1 was minipreped.

PCR

 * (7), (8), (10) was PCRed.


 * (7), (10) was PCRed twice (again).

These concentration suggest that PCR was not achieved. Low concentration of primer causes PCR fault. We should have mix well primer when we add water in primer tube. We are going to retry PCR of (7),(10) the next day.

To Do

 * measuring concentration of (7), (10) (see lab-note on 2 Sept.)
 * PCR and measuring concentration of (7), (10) (retry)
 * Restriction Enzyme, and PCR purification (8).

To Do
(5)
 * PCR
 * PCR purification kit

(5), (7), (10)
 * Restriction Enzyme
 * purification kit
 * ligation