Team:TorontoMaRSDiscovery/Notebook/April



=April 27, 2009=
 * 1) Received from Rosa (SPiT):
 * 2) *TM0785
 * 3) **Plasmid containing encapsulin
 * 4) **Recommend transfect into bacteria and re-sequence
 * 5) **See email note regarding sequence error
 * 6) *0.5 microliters TMG DNA 100 microgram/microliter
 * 7) **Use 0.4 microliter for 50 microliter PCR reaction =August 1, 2009=
 * 8) Overnight encapsulin cultures 1 and 6 (randomly selected) and the negative control were miniprepped
 * 9) The rest of the encapsulin cultures were stocked with 20% glycerol
 * 10) 6 digestions were done: Enc1, Enc6, Enc -ve, BB5, BB7, and C
 * 11) *16ul of BB5 plasmid was used
 * 12) *500ng of plasmid were used for the others
 * 13) The digestions were run on a 1.3% agarose gel in TAE (from here on, unless otherwise specified, all gels were 1.3% agarose)
 * 14) *BB5 was confirmed and all other parts were correct as well
 * 15) Overnight ligation of 7+Enc in the PCR machine