IBB Pune/25 June 2009

I) DISCUSSIONS WITH PRAVEEN SIR...


 * Ligation needs to be practised.


 * PCR-ligation-Transformation takes time to come. no colonies even after 16 hours.


 * Troubleshooting,
 * Kan too High? Try lowering KAn.
 * Ligation, try blunt end with the cohesive
 * Is PCR the right way to manna? Try transformation.
 * Proceed with the current thing, wait patiently for colonies to appear.

II)Plasmid isolation started from single colony from P4N.