Template:Team:KULeuven/26 August 2009/BlueLightReceptor


 * 1) The plates with ligation A (blp + GFP) were fetched from the blue light installation. There was no GFP signal. The following actions will be taken:
 * 2) * The plasmids will be purified from the colonies and will be sequenced using primer 2260.
 * 3) * Next time, we will probably put them in liquid cultures under the LED's while shaking gently. Also, other parameters that need to be considered are being researched.
 * 4) * They will not be exposed to the light as long anymore. We decided that 1h will be more than enough.
 * 5) * Possible bleaching?
 * 6) A colony from all three plates with the LigA-construct was taken and ented in liquid culture. This was needed to check whether the colonies were still alive.
 * 7) * culture 13/08
 * 8) * culture 14/08 (1)
 * 9) * culture 14/08 (2)
 * 10) By 5pm, one of the liquid cultures had grown and was miniprepped. 20&mu;l was sent for sequencing together with 5&mu;M of primer 2260.

4. Two electroporations were performed and plated on LB medium. One with LigC ( + ) and one with DNA.