Uppsala-Sweden/11 October 2009

Plasmid prep of PRPRAT and PRKRYT
data shown here: [[Media:091011_Plasmidprep.xls]]

Triparental mating
Today we put our antisense-RNA constructs in to Synecocystis via triparental mating. Protocol here: [[Media:Triparental+Mating.txt]]

Deviated in that we did not concentrate the Synecocystis culture and instead just it straight and at 10 times dilution, corresponding to 10 and 100 times dilution in the protocol.

Evaluating constructs in e.coli
The transformations made yesterday gave plenty of colonies and some were picked and verified in a colony PCR.