Team:Groningen/Notebook/15 August 2009

Wet
Some plates were found in the incubator again, Frans'? AC3 plated out 13/8/9 had one colony, put to fridge.

GVP Cluster
E. coli TOP10 cells grown on plate with IPTG and resuspended in saline solution (0.9% NaCl). Left is control, no Gas Vesicle Proteins (GVP), Right is with IPTG induced GVP. Picture is after one day.

Metal Accumulation
MymT transformations All below had colonies in the morning (plated out on LBAmp 14august2009) Below had colonies at the end of the day Codes were copied from plates and not interpreted. All plates with colonies were put to fridge, all without were put back to 37 °C.
 * E. coli TOP10 with pSB1AC3-MymT 1(6x) [GNK conc.]
 * E. coli TOP10 with pSB1AC3-MymT 2(6x) [GNK conc.]
 * E. coli TOP10 with pSB1AC3-MymT 1(6x) [GNK conc.] (more colonies than the one above)
 * E. coli TOP10 with pSB1AC3-MymT 1(3x) [GNK conc.]
 * E. coli TOP10 with pSB1AC3-MymT 2(3x) [GNK conc.]
 * E. coli TOP10 with pSB1AC3-MymT 1(6x) [GNK]