Team:Paris/24 August 2009

NoteBook
August 24th

 

Lab work
Ligation Tranfo OmpAsignal A28 : pSB1A3

15min 20° then transfo // O/N 16° gel purif A40 TolRII

Transformation check

D40+PSB1A3->Nter RFP-->worked

D41+PSB1A3->RFP-->worked

D42+PSB1A3->Cter RFP-->worked

negativ control OK

PCR on colonies

D40+PSB1A3->Nter RFP-->ok

D41+PSB1A3->RFP-->not ok but don't need

D42+PSB1A3->Cter RFP-->ok

negativ control OK