Team:Virginia/23 July 2009

Today

 * Ligated A09-10
 * ABP-HA E,P
 * pSB1C3 E,P
 * Versions 1, 2, and 3 reflect different ratios of vector to insert. 1:2, 1:6, 1:1 respectively.
 * Transformed A09-10 into DH5alphas
 * PCRed HA tag onto PCS
 * Ran gel of tagged PCS-HA
 * [[Image:PCS-HA.JPG‎]]
 * Product of PCR on PCS in attempt to add HA tag
 * Results indicate that there was no PCR product.