August/9 October 2009

We collected sequence data from the automated sequencer and used sequence alignment software to check the sequences against those obtained from the Parts Registry. The results were as follows: Senders: LuxI - mismatched LasI - OK RhlI - OK CinI - unconfirmed (DNA sample concentration too low?) Receivers: LuxR - OK LasR - mismatched RhlR - OK CinR - unconfirmed (DNA sample concentration too low?) The DNA for Cin signaling system will be transformed, amplified and purified to obtain at higher concentration and PCR sequencing repeated on them. As time is running out, for the final circuit we shall use parts sent from Chiba University to replace our faulty ones.

2.colony check sample  no. of colony 32                +++    X4                  ~30 X4                   7 70                    0    71                     4    74                      5    75                      7

3.min prep sample  conc. 1-15J   98 ng/uL 1-15L   87 43        169   44          172   52          114   65         214   67         148   67         126

4.transfromation saple 59, 72 , 68 , 69 chiba team parts ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9)

5.digation

K204058

K204062

K204072

K204077

K204078

↓ 37 degree, 2hr gel cut & purification ligation

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