August/23 August 2009

1.transformed check Checked the cell cultures transformed and plated out yesterday (8/22) for colony formation and made an approximate count of the number of colonies.

(ligation sample No.) (no. of colonies) 9                   1          10                   100~          11                   100~ inoculate to iquid medium(5mL + Amp 5uL or Kan 25uL)

2.digestion and ligation

'8/22 digestion sample ↓ gel electrophoresis UP  : ladder, No.8-1.2.3.4.5. , No.7-1,2,3,4,5, DOWN : ladder, 1-18L , 1-23J , 1-23L , No.3 , 1-2M , 2-8G ladder, 1-2M , 1-14H , 1-23L , No.6 gel cut ↓ purification by [QIAquick Nucleotide Removal Kit] ↓ ligation

ligation DNA       44 10* buffer 5 ligation  1 total     50uL

↓ 16°C 3~4hr ↓ transfromation

3.transfromation ligation sample ⑤⑫⑬⑭⑮ 2-18H 1-8E 1-4E 2-16N 2-16L

back to NOTES