August/14 October 2009

1. min prep sample     conc. 81             15 ng/uL 81             24  81              40  73              39  84              40  73              35  79              37  79              30  82              38  67              28  83              35  73              52  83              23  79              28      75              62  86              56  88              29  89              15  89              54  89              15

2.dation

K204097

K204098

K204099

K204100

K204101

K204102

↓ 37 degree, 2hr gel cut & purification ligation

3.min prep sample   conc. 72           64  ng/uL 72           41  72            35   91          153   91          201   91          126

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