Wisconsin-Madison/17 September 2009

September 17, 2009
'''GSMT+BB, GSMT+33, ProU+RFP sequencing

.5 uL DNA (100 ng)

1 uL Big Dye

1.5uL Big Dye Buffer

.25uL Primer (fwd or rev)

5.75uL H2O

Sequencing clean-up using magnetic beads

Confirm sequencing results that GSMT+BB, GSMT+33 and ProU+RFP worked.

rearanged plasmids in freezer

Plasmid Isolation of pBAD35

Cyano Talk with Daniel

Wrote abstract and sent

'''Inoculation:
 * pHawaii (strep)
 * pBAD35 (kan)

Clean and Ran Digestion Product

Digestion and Ligation
 * SDMT:pBAD18