Team:Cambridge/Project/ME02

= Melanin Pigment =

Background Design Characterisation Reference

Constructing the BioBrick
Biobrick

Our aim is to make the MelA gene into a biobrick as follows:



In order to do so, we had to remove forbidden restriction sites within the gene using primers and the in-fusion PCR technique. Primers were ordered as shown by the image below, to add the prefix and suffix and change the PstI restriction site from CTGCAG to CTGCAA:



The PCR's will be done in the following steps, in order to remove both sites and prepare the gene as a biobrick: