Team:Warsaw/Calendar-Main/26 August 2009

Making of the plac-RBS-llo-intA part Jarek

Tasks:  Separation of digested DNA in 0,8% agarose gel. Transformation competent E.coli strain with one of the DNA samples containing plac-RBS-llo-RBS-intA part Digestion of original pUC19-internaline A sample separetly with EcoRI and PstI endonuclease. 

Cloning switch 1 regulatory parts [ K177012-PcI.RBS.LacI, K177033-PcI.RBS.LacI.PcI.RBS.RFP.terminator, K177011-PLacI.RBS.cI.terminator, K177038-PLacI.RBS.cI.terminator.PLacI.RBS.GFP.terminator ] into two compatible low copy number plasmids of different antibiotic resistance Ania Tasks:   </li> </ul>