Team:Newcastle/Meetings/3 September 2009

03 September 2009 - iGEM Meeting

 * Chair: Mat
 * Students: Mat, Jess Goksel, James, Hanny, Arun
 * Minutes: James (in Jane's absence)
 * Instructors: Neil, Jen
 * Advisors: Morgan

Agenda

 * Review last week's action points
 * Lab summary
 * Questions
 * Next week's lab plan

Action Points during last meeting

 * Need some sequencing primers for each BioBrick
 * Tidy up some of the cloning strategies
 * Either put the virtual constructs on the hard drive of the computer in Phil's office OR place them onto a shared network
 * Need to review timeline
 * Each team needs to come up with a five minute summary of their sub-project
 * Ask Nigel Robinson's advice on how to help Cornell
 * E-mail UQ University and enquire about helping them with modelling
 * Negotiate swap with Cornell and enquire about modelling
 * Follow up Groningen's e-mail
 * T-shirts designs
 * Integration Vector?
 * Promoter library?

Reviewing last meeting's Action Points

 * Need some sequencing primers for each BioBrick
 * Tidy up some of the cloning strategies
 * Put current versions on the wiki, even if they are not finished; just to have them in one place.
 * Either put the virtual constructs on the hard drive of the computer in Phil's office OR place them onto a shared network
 * use Dropbox (getdropbox.com)
 * Need to review timeline
 * Students to ammend timeline.
 * Team members
 * Hanny has returned, after handing in her work.
 * Jane has left for a period of one month.
 * Jen has returned.
 * Each team came up with a five minute summary of their sub-project, James, Mat and Jess
 * 5 Minute kit has arrived.
 * Some of the PCRs are not working.
 * Use a different Taq.
 * Redesign primers, maybe bigger and without cut sites (2 days).
 * Use less genomic DNA.
 * More ethanol is needed (Chris is to be reminded next wednesday).
 * Only small gloves are currently available in the lab - we are to order some.
 * Ask Nigel Robinson about working with Cadmium, and an electromicroscope.
 * Ask Nigel Robinson's advice on how to help Cornell
 * Mat emailing him; he has indirectly assisted Groningen.
 * E-mail UQ University and enquire about helping them with modelling
 * Mat to email.
 * Modellers are to meet to discuss and work.
 * Negotiate swap with Cornell and enquire about modelling
 * Mat to email?
 * Modellers are to meet to discuss and work.
 * Follow up Groningen's e-mail
 * Mat to email?
 * T-shirts designs
 * Numbers of shits - 11 are going to Jamboree, plus other members, backups, etc.
 * Personalise some of the shirts - make name or initials look like plasmid ID.
 * Purple is a possible colour.
 * Design needed soon.
 * Poster
 * Can be no larger than A0 (1.2m2)
 * Look at possible design layouts.
 * Must be sent for printing 2 weeks before Jamboree.
 * Presentation
 * Mock presentations are to be given to:
 * The Bioinformatics research group on a Friday morning in late October.
 * Noel's student group in Sunderland.
 * Prestenation should be around 20 mins; in a 30 min time slot.
 * 10-20 slides.
 * Come up with our content, before designing slides
 * Use Camtasia to record models, etc.
 * Some parts are to be simplified (eg. gene names), more tech parts to be kept (eg. equations).
 * Try to focus on the Biology, not the 'experience'.
 * Integration Vector?
 * Promoter library?
 * Mat is working under Neil and Jen's supervision on this in the lab; cloning into pgfp, etc.
 * Sequence the AND gate?
 * Needed for Tuesday.
 * Boston
 * Research ESTA for USA trip.
 * Neil to send out full details of flights and hotels.
 * Noel's microscope in Sunderland.
 * Goksel to be trained.
 * Lab rota
 * New rota to be devised.
 * Current 'free-for-all' cannot continue.
 * Teams are to take time out of lab to work on t-shirts, posters, modelling, etc.
 * Labwork
 * Lab work is to continue at fast pace.
 * Shared labbooks are to remain in the lab.
 * Gel UV images are to be placed in Dropbox.
 * Print out photos of gels, etc, and stick into lab books.
 * Use the lab scanner for copying lab book content for wiki.
 * Neil to send off Plasmids.
 * Various PCR primers and enzymes have arrived.