Team:KULeuven/24 July 2009

=Progress=
 * Received E. Coli odourless strain, put in 4C

Vanillin Receptor
Possible receptor is the VirA/VirG system from Agrobacterium, the VirA protein will bind to phenolic compounds and induce transcription of vir genes by phosphorylation of VirG. Protein ChvE results in a broader response to phenolic compounds. Papers:

[1] S C Winans, Two-way chemical signaling in Agrobacterium-plant interactions, Microbiol Mol Biol Rev. 1992 March; 56(1): 12-31 [2] Wen-Tao Peng, Yong-Woog Lee, and Eugene W. Nester, The Phenolic Recognition Profiles of the Agrobacterium tumefaciens VirA Protein Are Broadened by a High Level of the Sugar Binding Protein ChvE, Journal of Bacteriology, November 1998, p. 5632-5638, Vol. 180, No. 21 [3]Yong-Woog Lee, Shouguang Jin, Woong-Seop Sim, Eugene W. Nester, The sensing of plant signal molecules by Agrobacterium: genetic evidence for direct recognition of phenolic inducers by the VirA protein, Gene, Volume 179, Issue 1, 1996, Pages 83-88, ISSN 0378-1119, DOI: 10.1016/S0378-1119(96)00328-9. [4]Y W Lee, S Jin, W S Sim, and E W Nester,Genetic evidence for direct sensing of phenolic compounds by the VirA protein of Agrobacterium tumefaciens, Proc Natl Acad Sci U S A. 1995 December 19; 92(26): 12245–12249.

Vanillin synthesis
Received mail from U. Edinburgh: We will receive:
 * Sam5; no part number, Pst1 site removed
 * Sam8; coding sequence without RBS
 * ComT; coding sequence without RBS
 * ech; RBS + ech, in pSB1A2,
 * fcs; RBS + fcs, in pSB1A2,