Uppsala-Sweden/25 August 2009

Assembly
The plasmids holding our bio bricks were digested according to the file below.

[[Media:assembly_Plasmid_digestion.xls]]

The concentrationa measured after digestion and purification (NucleoSpin PCR clean up kit)

[[Media:digest_purification_measur_09825.txt]]

The volumes used for ligation is shown in the file below "digest_purification_measur_09825.htm"

[[Media:Ligation voulume calcuator_pSB1AK3_090825.xls]]

This file "Biobricks and vectors" contains a list of ready bio bricked genes.

[[Media:Biobricks and vectors.xls]] --Flormane 18:16, 25 August 2009 (UTC)

Oligos for RNAi and Primers for PirA ordered
[[media:Primer_Biolegio_20090825.xls]]

--Karl.brune 18:37, 25 August 2009 (UTC)

Evaluating competence
Three sets of cells were transformed with 1 l each pUC19 control plasmid. The cells were Hsin Ho's, our earlier and our new cells. The usual transformation procedure was followed.' --Flormane 13:09, 26 August 2009 (UTC)