Team:Warsaw/Calendar-Main/2 September 2009

Assembly of endosomal detection operon Marcin Task 1: Restriction digest of followed costruct:  BBa_K177037 from the  pSB1A3 plasmid Methods: Reaction mixture composition: 1. &mu;l purified plasmid DNA product 1 &mu;l XbaI (Fermentas) 1 &mu;l PstI (Fermentas) 2 &mu;l Buffer Tango (Fermentas) 15 &mu;l MQ water Digest was performed about six hours and subsequently thermal inactivated Results All isolated plasmids have no insert</li></ul> Task 2: Prepare the bacterial cultures for isolation of on  pSB1A3</a> plasmid containing  BBa_K177035</a> </ul>

Acquiring the internaline A gene from Listeria monocytogenes st. EDG-e genome
Jarek

Tasks:
 * Preparation of PCR reaction with L.monocytogenes st. EGD-e genome DNA
 * Separation of PCR products on the 0,8% agarose gel
 * Acquiring the pure DNA sample from PCR reaction with Clean-Up kit

Cloning of the mgtc promoter into the pSB1A3 plasmid Kamil

Tasks:  Colonies transfer</li> Liquid cultures establishment</li> </ul>

Methods:  Appropriate colonies (both of them) were transferred to a new plate containing ampicilin</li> Liquid cultures were established in 5 ml LB medium supplemented with ampicilin and incubated overnight with areation</li> </ul>

Cloning of the cro-box into the pSB1A3 plasmid Kamil

Tasks:  Colonies transfer</li> Liquid cultures establishment</li> </ul>

Methods:  Appropriate colonies (all 7 of them) were transferred to a new plate containing ampicilin</li> Liquid cultures were established in 5 ml LB medium supplemented with ampicilin and incubated overnight with areation</li> </ul>

Cloning switch 1 regulatory parts [ <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K177012">K177012</a>-PcI.RBS.LacI, <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K177033">K177033</a>-PcI.RBS.LacI.PcI.RBS.RFP.terminator, <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K177011">K177011</a>-PLacI.RBS.cI.terminator, <a href="http://partsregistry.org/Part:BBa_K177038">K177038</a>-PLacI.RBS.cI.terminator.PLacI.RBS.GFP.terminator ] into two compatible low copy number plasmids of different antibiotic resistance Ania Tasks: <ul> <li> </li> </ul>