Team:IBB Pune/project/Results

Characterization of And Gates: Even as the wiki freezes we are characterizing Const.Prom-LacO-mRFP for its expression. We hope we get positive results.

We carried out the following transformations successfully:

Native Biobricks


 * 1) pLL promoter               - BBa_I746365
 * 2) GFP coding sequence        - BBa_E0040
 * 3) RFP coding sequence
 * 4) GFP Translational unit
 * 5) RFP Translational unit
 * 6) Enhanced CFP
 * 7) Fast GFP
 * 8) Constitutive promoter      -BBa_J23102
 * 9) Promoter + RBS
 * 10) T7 promoter
 * 11) pLuxR
 * 12) Double terminator
 * 13) RBS
 * 14) Constitutive LuxI
 * 15) LuxR Translational unit

OUR PARTS


 * 1) YcdB
 * 2) TorA
 * 3) Lac Operator

Assembly


 * 1) Const. Promoter-LacO
 * 2) PluxR- LuxI
 * 3) {Promoter + RBS}-GFP
 * 4) {Promoter + RBS}-TorA
 * 5) {Promoter + RBS}-Ycdb
 * 6) luxR-PluxR-{RBS-LuxI}
 * 7) Promoter + RBS
 * 8) YcdB-GFP
 * 9) TorA-GFP
 * 10) {Promoter + RBS}-LuxR
 * 11) {Promoter + RBS}-YcdB-GFP
 * 12) {Promoter + RBS}-TorA-GFP
 * 13) Const. Promoter-LuxR

Failed Transformations

Backbones


 * 1) pSB1AK3 with CcdB gene
 * 2) pSB1T3 with CcdB gene
 * 3) pSB1C3 with CcdB gene
 * 4) pSB1K3 with CcdB gene
 * 5) pSB1A3 with CcdB gene
 * 6) pSB1AC3 with CcdB gene
 * 7) pSB1AK3 with mRFP
 * 8) pSB1T3 with mRFP
 * 9) pSB1C3 with mRFP
 * 10) pSB1K3 with mRFP
 * 11) pSB1A3 with mRFP
 * 12) pSB1AC3 with mRFP

Native Biobricks


 * 1) Lysis Cassette
 * 2) Constitutive GFP
 * 3) COnstitutive CFP

GEL PICTURES