Team:Wash U/Chinese/Project



摘要
我们项目的目标是最大限度地光合生产率文化的红假单胞菌下的高，低光照强度的生物反应器的综合调控的规模，捕光天线. 我们选择进行这样一个项目在河米由于其良好的光合特征和遗传系统. 该天线系统功能扩展频谱的光进行光合作用可吸收不同波长比反应中心. 从本质上讲，它是像周围的菜的主要接收任何天线. 海洋细菌，如河米，形成非常大的天线复合吸收自然光环境下也有很大的竞争光子. 其结果是，饱和光合机制在相当低的光照强度的一种合成非竞争的环境，如生物反应器. 这将导致高达95 ％的附带光子散失的热量或荧光的细菌在外部的生物反应器，通过这个过程被称为非光化学猝灭（热耗散） （ Mussgnug等. ， 2007年）. 从本质上讲，这些光子正在降低热耗散浪费轻渗透到生物反应器和挨饿细胞内部的光子. 一种方法已被证明是提高光合效率，减少捕光天线尺寸（ Polle等. ， 2002年， Mussgnug等. ， 2007年）. 虽然，目前的办法，为此依靠基因敲除，因此很难精确地控制视野下的代谢工程和合成生物学. 我们的意图是建立一个更有活力的制度，以不同的天线大小，依赖于偶然的光照强度，并且可以非常容易地优化利用合成生物学的原则. 这个系统应该导致细菌在外部的生物反应器表达少捕光天线蛋白比细胞内部，减少热耗散，同时保持高吸光度附带光子. 我们将集中在改变的数量，捕光复合2 （ LH2 ）通过调节pucB /阿基因代码，这两个亚单位的复杂. LH2最大限度地吸收光子的波长为842 nm和渠道其能源LH1和反应中心. 比例LH2复合物，以LH1自然范围从3.0至6.7在不同光线条件（ Scheuring等. ， 2005年）. 我们建议，如果这一比率到LH1是LH2改为从0-7或以上的回应附带光照强度，那么，光合效率和生产力，文化的河细菌可能是最大的. 回页首

分析
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结果
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结论
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