Virginia Commonwealth/25 June 2009

Results

 * At 9:30 am the plate with the e.coli transformed with part pSB3K3 on LB+KAN grew approximately 50 small colonies.
 * The plate growing the pSB3K3+BBa_J23102+E0240 assembly on LB+KAN grew approximately 50 small colonies. Neither growth expressed fluorescence.

Tasks

 * 1 Promoter Design Meeting
 * we discussed different methods of promoter design.
 * Using a paper that charted the % variance in the -10/-35 sigma binding region, we will design 16 promooters by changing the column with the smallest variances. Promoters will be documented upon completion.
 * After designing 16 promoters, we will change multiple columns at the same time, creating several more promoters to test.
 * Additional design and testing ideas include:
 * Creating and changing an 'up element' and testing how it changes the strength (more reading is necessary since this is a field of uncharted waters).
 * Adding the up element to our promoters and find the bonding strength of the sigma factors (sigma 70, sigma 38, and sigma 34).
 * Adding positive and negative controls to each set up and test for strength and if anything was altered or affected in any way by the addition. A design tree for this idea can be found on page 14 of the lab notebook.

Wetlab

 * Colonies from the pSB3K3 plate were picked and grown overnight in LB+KAN culture.