Team:Newcastle/Labwork/29 September 2009

=Formal Lab Session - 29th September 2009=

Today we did miniprep for the ten ON cultures. Run them on thel gel.

After the results we did a restriction digest with HindIII and BamHI to find out the right colony.

However we did not get good results and we could not find the right colony we were looking for.

Introduction

 * Two transformations need to be done today, one for psc-pMutin4 and another for kinA-pGFP-rrnB

Transformation for E.coli

 * Followed Phil's protocal.

Digest the Mini Prep result for smtA:pMutin4 transformation
dd H2O                    7ul 10X fast digest buffer    2ul Fast BamHI              0.5ul Fast HindIII            0.5ul Mini Prep DNA            10ul --                           20ul
 * This transformation was carried out by Matt and Arun.
 * HindIII and BamHI were used for digestion.
 * 37C 1 hour.
 * Run the sample on 0.8% agarose gel.

Conclusion

 * After the digestion of smtA:pMutin4 Mini result, the 10 strains are not the right clone.