August/26 August 2009

Conducted DNA Miniprep on the following cell growth cultures from 8/25. Results of Nanodrop concentration check:

ng/ul    260/280    260/230 1-8E(A)    16.3       1.98       1.44 1-8E(B)    15.1       1.91       1.31

Checked the culture plates transforLAmed with plasmids (12) through (15) left in overnight incubation from 8/25. No colonies were found, hence transformation of plasmids (12) through (15) were all repeated.

Checking of assembled parts uptaken by plated cell cultures was conducted by electrophoresis. The following parts were checked: 10-A, 10-B, 10-C, 10-D, 10-E, 9-A, 9-B. Results were not satisfactory so another electrophoresis run will be repeated tomorrow.

DNA treated with restriction enzymes, reacted overnight then put in cold storage from 8/24 and 8/25 were taken out and run through electrophoresis, then extracted to be purified. The parts labelled (8), (10), as well as mOrange, YFP, CFP were all run through electrophoresis but only the YFP and CFP parts had well-defined vector and insert bands so only these were cut out, purified and mixed with ligase then left overnight to react at 16 degs.

20 petri dishes of LA culture medium were prepared.

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