Virginia Commonwealth/22 July 2009

Results
Maria and Afton Trentay
 * Overnight cultures were successful
 * However, vials had noticeable amounts of white E. coli at the bottom of the tube.
 * Flow Cytometer was repaired
 * Samples cannot be tested until protocols for using and maintaining the machine are figured out
 * Plated bacteria are still not expressing GFP and RFP

Tasks
Maria and Afton Trentay 19:57, 22 July 2009 (UTC)
 * Dilute E. coli into fresh LB + Cm in preparation for testing with the flow cytometer
 * nothing was tested today with the cytometer
 * Grow all BioBrick parts in 5 mL of LB + Cm overnight
 * 100 microliters was added to broth
 * plastic tubes had tops, so tops were loosened during the incubation period
 * cells were out of the -80 degree Celsius freezer for a prolonged amount of time, so viability is lower than usual.
 * If nothing grows, BioBrick DNA will be reinserted into fresh NEB 10 beta through electrotransformation.
 * Retransform J23100 w/ J06702
 * Transform NEB 10 beta and DB 3.1 to check for electrocompetency
 * Organize notebook and running draft

Wetlab
Maria and Afton Trentay 20:51, 22 July 2009 (UTC)
 * Dilute overnight cultures
 * 1 mL of sample was diluted with 6 mL of LB + Cm
 * only seven samples were saved however, most of these parts were recultured and the flow cytometer was not used today, so these cell cultures most likely will not be kept
 * Samples from cryogenic stocks were grown up in LB:
 * DB 3.1 (2 vials)
 * Samples from cryogenic stocks were grown up in LB + Amp:
 * pSB4C5
 * Samples from cryogenic stocks were grown up in LB + Cm:
 * Transformation of electrocompetent E. Coli stock and BioBrick with promoter J23100 and RET J06702