Template:Team:KULeuven/10 September 2009/VanillinProduction


 * Gel electrophoresis of TER restriction
 * The vector for the TER is Psb1AK3 and has 3100bp, with an insert of ±3200 bp
 * Gel extraction
 * Created a motherplate from EF1
 * Ligated EF restriction + TER restriction
 * Transferred the SAMS 1 and SAMS 2 colonies to liquid medium