August/21 August 2009

transformation
to get new plasmid
 * ⑤(RBS+rhlR+terminator+promoter(reg))
 * ⑦（RBS＋cinR)
 * ⑧（RBS＋luxI)

check the result of transformation
Checked the cell cultures transformed and plated out yesterday (8/10) for colony formation and made an approximate count of the number of colonies.

(plate number)-(location on plate) (no. of colonies) 2-8G                        4 2-21F                       0 2-6O                        2 2-13N                      <15 ⑤                         0

restriction and digestion
K204009

K204010

K204011

↓ 37°C, 2hr

↓ gel electrophoresis gel cut

'8/22 purification by [QIAquick Nucleotide Removal Kit] ↓ ligation ligation DNA       44 10* buffer 5 ligation  1 total     50uL ↓ 16°C, 4hr

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