Uppsala-Sweden/7 September 2009

Plasmid Preps
Plasmid Prep with Sigma Gene Elute was performed with the following samples:

Subunit E2
 * 1) E21-1
 * 2) E22-3
 * 3) E23-3
 * 4) E24-1

Subunit E1 alpha
 * 1) E1A1-1
 * 2) E1A1-2
 * 3) E1A2-2
 * 4) E1A2-3
 * 5) E1A3-1
 * 6) E1A4-2

Subunit E1 beta
 * 1) E1B1-3
 * 2) E1B2-3
 * 3) E1B3-1
 * 4) E1B4-2

B0015, B0034-pirA, R0011-B0034, B0034-Yadh2-B0015
 * 1) T-1 - inoculation failed, new inoculation was performed
 * 2) RP-2
 * 3) RR-4
 * 4) RR-5
 * 5) RYT-1
 * 6) RYT-2

Furthermore these were prepped (Lux System etc.)

A340620, C0621, J237034
 * 1) A340620
 * 2) C0621
 * 3) J237034

and these here:
 * 1) R0062 (A)
 * 2) RARP 4, 5 ,7 (CH)

Plasmid Prep with Sigma Gene Elute was performed with the following samples:

RBS-pdc-RBS-ADH2(z) Three plasmid preps were performed for the large construct RBA-pdc-RBS-ADH2(z). With the final concenttations (100-td) [[Media:Plasmidprep_090907.TXT]]

The result was evaluated by cutting with EcoR1 and PstI and running on a gel.



--Flormane 06:27, 8 September 2009 (UTC)

--Karl.brune 14:12, 7 September 2009 (UTC)

Report for the above preps: [[Media:Plasmidprep_090907.txt]] --Anders 17:49, 7 September 2009 (UTC)

2nd Plasmid Prep
Prep of the B0015 (AC3) culture from this morning

--Karl.brune 21:50, 7 September 2009 (UTC)

Pouring Plates
I poured plates ( Kanamycin and Chloramphenicol ), stored at iGEM fridge

--Karl.brune 21:50, 7 September 2009 (UTC)

Inoculation for PP

 * 1) pSB1A3
 * 2) pSB1AC3 (2)
 * 3) pSB1AK3 (2)
 * 4) B0034 (A) (2)
 * 5) I13504 (A) (2)

Overnight at 37C, 250RPM in 6ml LB with specific antitbiotic

--Karl.brune 21:50, 7 September 2009 (UTC)