Team:Waterloo/Notebook/strainlist

Strain list
{| width="40%" border="1" cellpadding="2" !Location!!Strain #!!Name!!Chassis/Strain!!Plasmid Backbone!!Registry Part!!Antibiotic Resistance!!Description!!Reference Date


 * iGEM strain box 1
 * 1
 * rTT + PocI
 * DH5α
 * pMA (GeneArt)
 * BBa_K093003
 * Amp100
 * Synthesized. Contains BioBrick prefix and suffix. Reverse orientation transcriptional terminator R0015 with λ Pr promotor.
 * 2008:09:09
 * 2008:09:09


 * iGEM strain box 1
 * 2
 * rPlacTT
 * DH5α
 * pMA (GeneArt)
 * BBa_K093004
 * Amp100
 * Synthesized. Contains BioBrick prefix and suffix. Reverse BBa_R0011 and transcriptional terminator BBa_B0015.
 * 2008:09:09
 * 2008:09:09


 * iGEM strain box 1
 * 3
 * PmeI
 * DH5α
 * pMA (GeneArt)
 * BBa_K093002
 * Amp100
 * Synthesized ORF. Contains BioBrick prefix and suffix.
 * 2008:09:09
 * 2008:09:09


 * iGEM strain box 1
 * 4
 * rfp'-14
 * DH5α
 * pSB1A2
 * BBa_K093005
 * Amp100
 * RFP (BBa_E1010) with RBS (BBa_E0034) colony 14.
 * 2008:09:09
 * 2008:09:09


 * iGEM strain box 1
 * 5
 * rfp''-2
 * DH5α
 * pSB1AK3
 * Amp100, Kan25
 * RFP (BBa_E1010) with RBS (BBa_E0034) and transcriptional terminator (BBa_B0015) colony 2. Might need testing
 * 2008:09:09
 * RFP (BBa_E1010) with RBS (BBa_E0034) and transcriptional terminator (BBa_B0015) colony 2. Might need testing
 * 2008:09:09


 * iGEM strain box 1
 * 6
 * Pconst-rfp''-1
 * DH5α
 * pSB1A2
 * BBa_K093012
 * Amp100
 * RFP (BBa_E1010) with RBS (BBa_E0034) and transcriptional terminator (BBa_B0015) under a constitutive promoter (BBa_J23118). Might need testing.
 * 2008:09:09
 * 2008:09:09


 * iGEM strain box 1
 * 7
 * pRecA-3
 * DH5α
 * psb2k3
 * BBa_K093000
 * Kan25
 * Engineered RecA promoter with lexA consensus sequence.
 * 2008:10:17
 * 2008:10:17


 * iGEM strain box 1
 * 8
 * Pconst-rfp' 1
 * DH5α
 * psb1a2
 * Amp100
 * RFP (BBa_E1010) with RBS (BBa_E0034) under a constitutive promoter (BBa_J23118)
 * 2008:10:17
 * RFP (BBa_E1010) with RBS (BBa_E0034) under a constitutive promoter (BBa_J23118)
 * 2008:10:17


 * iGEM strain box 1
 * 9
 * c0051'
 * DH5α
 * psb1a2
 * BBa_K093006
 * Amp100
 * BBa_C0051 Repressor for Lambda Pr promoter with RBS (BBa_E0034). Never got sequencing results of prefix. Rest was good
 * 2008:10:17
 * 2008:10:17


 * iGEM strain box 1
 * 10
 * PmeI - 14
 * DH5α
 * psb2k3
 * BBa_K093002
 * Kan25
 * PmeI cloned into BioBrick vector
 * 2008:10:17
 * 2008:10:17


 * iGEM strain box 1
 * 11
 * PrecA RFP'
 * DH5α
 * pSB2K3
 * BBa_K093009
 * Kan25
 * RFP (BBa_E1010) with RBS (BBa_E0034) under the control of BBa_K093000 promoter
 * 2008:10:17
 * 2008:10:17


 * iGEM strain box 1
 * 12
 * PmeI'
 * DH5α
 * pSB1A2
 * BBa_K093013
 * Amp100
 * PmeI (BBa_K093002) with RBS (BBa_E0034)
 * 2008:11:04
 * 2008:11:04


 * iGEM strain box 1
 * 13
 * Pr RFP'
 * DH5α
 * psb1a2
 * BBa_K093010
 * Amp100
 * RFP (BBa_E1010) with RBS (BBa_E0034) under Lamda Pr promoter (R0051)
 * 2008:11:04
 * 2008:11:04


 * iGEM strain box 1
 * 14
 * T7 gene 6
 * DH5α
 * pSB1A2
 * BBa_K093001
 * Amp100
 * good one, correct stop codon taataa
 * 2009:02:13
 * 2009:02:13


 * iGEM strain box 1
 * 15
 * PkNG101
 * DH5α λ pir
 * Strep100
 * Only replicates in λ pir lysogen (r6k origin of replication) http://bccm.belspo.be/db/lmbp_plasmid_details.php?NM=pKNG101
 * 2009:02:xx
 * Strep100
 * Only replicates in λ pir lysogen (r6k origin of replication) http://bccm.belspo.be/db/lmbp_plasmid_details.php?NM=pKNG101
 * 2009:02:xx


 * iGEM strain box 1
 * 16
 * H1-4 pJET
 * DH5α
 * pJET
 * Amp100
 * E. coli melA genomic DNA for homologous recombination (“left sequence”/H1) and attP site downstream
 * 2009:03:18
 * E. coli melA genomic DNA for homologous recombination (“left sequence”/H1) and attP site downstream
 * 2009:03:18


 * iGEM strain box 1
 * 17
 * CI'' pJET
 * DH5α
 * pJET
 * Amp100
 * CI repressor for lamda Pr promoter. BBa_C0051 with RBS (BBa_E0034) and transcriptional terminator (BBa_B0015) in pJET (made by crossover PCR)
 * 2009:03:xx
 * CI repressor for lamda Pr promoter. BBa_C0051 with RBS (BBa_E0034) and transcriptional terminator (BBa_B0015) in pJET (made by crossover PCR)
 * 2009:03:xx


 * iGEM strain box 1
 * 18
 * pFB9002
 * DH5α lamda pir
 * pfb9001
 * Strep 100
 * E. coli melA genomic DNA for homologous recombination (“left sequence”/H1) and attP site downstream
 * 2009:03:xx
 * E. coli melA genomic DNA for homologous recombination (“left sequence”/H1) and attP site downstream
 * 2009:03:xx


 * iGEM strain box 1
 * 19
 * pJET H2
 * DH5α
 * pJET
 * Amp100
 * E. coli melA genomic DNA for homologous recombination (“right sequence”/H2) and attP site upstream
 * 2009:04:09
 * E. coli melA genomic DNA for homologous recombination (“right sequence”/H2) and attP site upstream
 * 2009:04:09


 * iGEM strain box 1
 * 20
 * Pfb9003
 * DH5α lamda pir
 * pfb9001
 * Strep100
 * E. coli melA genomic DNA for homologous recombination (“right sequence”/H2) and attP site upstream
 * 2009:04:23
 * E. coli melA genomic DNA for homologous recombination (“right sequence”/H2) and attP site upstream
 * 2009:04:23


 * iGEM strain box 1
 * 21
 * pfb9004
 * DH5α lamda pir
 * pfb9001
 * Strep100
 * Contains two attP sites flanked by E. coli melA genomic DNA for homologous recombination (H1 and H2)
 * 2009:04:23
 * Contains two attP sites flanked by E. coli melA genomic DNA for homologous recombination (H1 and H2)
 * 2009:04:23


 * iGEM strain box 1
 * 22
 * pJET rfp-1
 * DH5α
 * pJET
 * Amp100
 * insert: Pconst (BBa_J23118) rfp (BBa_E1010) EcoRV fragment for propagating pJET
 * 2009:04:24
 * insert: Pconst (BBa_J23118) rfp (BBa_E1010) EcoRV fragment for propagating pJET
 * 2009:04:24


 * iGEM strain box 1
 * 79
 * pJET rfp-2
 * DH5α
 * pJET
 * Amp100
 * insert: Pconst rfp EcoRV fragment
 * 2009:04:24
 * insert: Pconst rfp EcoRV fragment
 * 2009:04:24


 * iGEM strain box 1
 * 81
 * pJET rfp-3
 * DH5α
 * pJET
 * Amp100
 * insert: Pconst rfp EcoRV fragment
 * 2009:04:24
 * insert: Pconst rfp EcoRV fragment
 * 2009:04:24


 * iGEM strain box 1
 * 23
 * RBS
 * DH5α
 * pSB1A2?
 * B0034
 * Amp100
 * Ribosome binding site (Elowitz)
 * Ribosome binding site (Elowitz)


 * iGEM strain box 1
 * 24
 * Pconst55%
 * DH5α
 * BBa_J61002
 * BBa_J23118
 * Amp100
 * Constitutive promoter. Expression level is 55% in comparison with the highest expression levels from this Pconst family (ex. wildtype promoter)
 * Constitutive promoter. Expression level is 55% in comparison with the highest expression levels from this Pconst family (ex. wildtype promoter)


 * iGEM strain box 1
 * 25
 * TT
 * DH5α
 * pSB1AK3
 * BBa_B0015
 * Amp100, Kan25
 * Default double transcriptional terminator
 * Default double transcriptional terminator


 * iGEM strain box 1
 * 26
 * rfp
 * DH5α
 * pSB2K3
 * E1010
 * Kan25
 * Red fluorescent protein (shows up pink even without appropriate wavelength of light and filter)
 * Red fluorescent protein (shows up pink even without appropriate wavelength of light and filter)


 * iGEM strain box 1
 * 27
 * Pseudomonas mendocina
 * kr 1
 * Wrong strain for cloning PmeI (received from Paul Hatzinger at Shaw Environmental NJ). Degrades N-nitrodimethylamine (NDMA). Appl Environ Microbiol. 2006 Oct;72(10):6693-8. Epub 2006 Sep 1
 * 2008:08:xx
 * Wrong strain for cloning PmeI (received from Paul Hatzinger at Shaw Environmental NJ). Degrades N-nitrodimethylamine (NDMA). Appl Environ Microbiol. 2006 Oct;72(10):6693-8. Epub 2006 Sep 1
 * 2008:08:xx
 * Wrong strain for cloning PmeI (received from Paul Hatzinger at Shaw Environmental NJ). Degrades N-nitrodimethylamine (NDMA). Appl Environ Microbiol. 2006 Oct;72(10):6693-8. Epub 2006 Sep 1
 * 2008:08:xx


 * iGEM strain box 1
 * 28
 * Plac
 * DH5α
 * pSB1A2
 * BBa_R0010
 * Amp100
 * Wildtype LacI repressable promoter
 * Wildtype LacI repressable promoter


 * iGEM strain box 1
 * 29
 * PtetR
 * DH5α
 * pSB1A2
 * BBa_R0040
 * Amp100
 * Promoter that is repressed by tetR and derepressed by tetracyclin.
 * Promoter that is repressed by tetR and derepressed by tetracyclin.


 * iGEM strain box 1
 * 30
 * CI
 * DH5α
 * pSB1A2
 * BBa_C0051
 * Amp100
 * CI repressor for λ Pr promoter
 * CI repressor for λ Pr promoter


 * iGEM strain box 1
 * 31
 * rTT
 * DH5α
 * pSB1A2
 * B0025
 * Amp100
 * Reverse transcriptional terminator (BBa_B0015)
 * 2008:xx:xx
 * 2008:xx:xx


 * iGEM strain box 1
 * 32
 * Taq
 * DH5α
 * Amp100
 * Taq strain from Moffatt lab for purifying taq
 * 2008:08:xx
 * Amp100
 * Taq strain from Moffatt lab for purifying taq
 * 2008:08:xx


 * iGEM strain box 1
 * 33
 * Pconst+
 * DH5α
 * pSB1A2
 * BBa_J23119
 * Amp100
 * Wildtype promoter─strongest of this promoter family (BBa_J23100-BBa_J23119)
 * Wildtype promoter─strongest of this promoter family (BBa_J23100-BBa_J23119)


 * iGEM strain box 1
 * 34
 * LacI
 * DH5α
 * pSB1A2
 * BBa_C0012
 * Amp100
 * LacI repressor for Plac Promoter (BBa_R0010) with LVA degredation tag
 * LacI repressor for Plac Promoter (BBa_R0010) with LVA degredation tag


 * iGEM strain box 1
 * 35
 * Pconst4%
 * DH5α
 * BBa_J61002
 * BBa_J23109
 * Amp100
 * Constitutive promoter. Expression level is 4% in comparison with the highest expression levels from this Pconst family (ex. wildtype promoter)
 * Constitutive promoter. Expression level is 4% in comparison with the highest expression levels from this Pconst family (ex. wildtype promoter)


 * iGEM strain box 1
 * 36
 * TTbi
 * DH5α
 * pSB1AK3
 * BBa_B0014
 * Amp100,Kan25
 * Double terminator─not as strong as BBa_B0015 but more even efficiency for forward and reverse use.
 * Double terminator─not as strong as BBa_B0015 but more even efficiency for forward and reverse use.


 * iGEM strain box 1
 * 37
 * Pconst100%
 * DH5α
 * BBa_J61002
 * BBa_J23100
 * Amp100
 * Constitutive promoter. Expression level is 100%─the highest expression level for this Pconst family (i.e., wildtype promoter)
 * Constitutive promoter. Expression level is 100%─the highest expression level for this Pconst family (i.e., wildtype promoter)


 * iGEM strain box 1
 * 38
 * PlacCI''
 * DH5α
 * pSB2K3
 * Kan25
 * cI repressor (BBa_C0051) with RBS (BBa_E0034) and transcriptional terminator (BBa_B0015) under the promoter Plac (BBa_R0011).
 * 2009:04:xx
 * cI repressor (BBa_C0051) with RBS (BBa_E0034) and transcriptional terminator (BBa_B0015) under the promoter Plac (BBa_R0011).
 * 2009:04:xx


 * iGEM strain box 1
 * 39
 * pTC300
 * DH5α
 * pUC19
 * Cm10, Kan25
 * Strain containing Kanamycin resistance gene, and SacB negative selection gene flanked together by BamHI sites
 * 2009:05:30
 * Strain containing Kanamycin resistance gene, and SacB negative selection gene flanked together by BamHI sites
 * 2009:05:30


 * iGEM strain box 1
 * 40
 * DB 3.1
 * DB 3.1
 * CCDB resistant strain, contains mutation in GyrA
 * 2009:05:30
 * CCDB resistant strain, contains mutation in GyrA
 * 2009:05:30
 * CCDB resistant strain, contains mutation in GyrA
 * 2009:05:30


 * iGEM strain box 1
 * 41
 * DH5α
 * DH5 alpha
 * For making competent cells. Default strain for
 * 2007:xx:xx
 * For making competent cells. Default strain for
 * 2007:xx:xx
 * For making competent cells. Default strain for
 * 2007:xx:xx


 * iGEM strain box 1
 * 42
 * pFB9006
 * DH5α λ pir
 * pFB9001
 * Strep100/Kan20
 * pFB9005+KanSac. pFB9001 containing H2 homologous region to E. coli melA gene. Does not contain H1 region.
 * 2009:06:xx
 * pFB9005+KanSac. pFB9001 containing H2 homologous region to E. coli melA gene. Does not contain H1 region.
 * 2009:06:xx


 * iGEM strain box 1
 * 43
 * pSB1A3
 * DH5α
 * pSB1A3
 * Amp 100
 * BioBrick vector from 2009 registry.
 * 2009:06:16
 * BioBrick vector from 2009 registry.
 * 2009:06:16


 * iGEM strain box 1
 * 44
 * luxI''
 * DH5α
 * pSB1AK3
 * Amp100, Kan 25??
 * luxI (BBa_C0061) +RBS (BBa_E0034) and transcriptional terminator (BBa_B0015)
 * 2007:xx:xx
 * luxI (BBa_C0061) +RBS (BBa_E0034) and transcriptional terminator (BBa_B0015)
 * 2007:xx:xx


 * iGEM strain box 1
 * 45
 * PlaclasR''
 * DH5α
 * pSB1A2
 * Amp100
 * lasR (C0079) with RBS (BBa_E0034) and TT (BBa_B0015) under the control of a lac promoter (unsure which promoter - would need testing/sequencing)
 * 2007:xx:xx
 * lasR (C0079) with RBS (BBa_E0034) and TT (BBa_B0015) under the control of a lac promoter (unsure which promoter - would need testing/sequencing)
 * 2007:xx:xx


 * iGEM strain box 1
 * 46
 * Plux rfp'
 * DH5α
 * pSB2K3
 * Kan25
 * RFP (BBa_E1010) with RBS (BBa_E0034) under Plux promoter
 * 2007:xx:xx
 * RFP (BBa_E1010) with RBS (BBa_E0034) under Plux promoter
 * 2007:xx:xx


 * iGEM strain box 1
 * 47
 * Plas rfp'
 * DH5α
 * pSB2K3
 * Kan25
 * RFP (BBa_E1010) with RBS (BBa_B0034) under Plas promoter (BBa_R0079)
 * 2007:xx:xx
 * RFP (BBa_E1010) with RBS (BBa_B0034) under Plas promoter (BBa_R0079)
 * 2007:xx:xx


 * iGEM strain box 1
 * 48
 * Ptet rfp'
 * DH5α
 * pSB2K3
 * Kan25
 * RFP (BBa_E1010) with RBS (BBa_B0034) under Ptet promoter (BBa_R0040)
 * 2007:xx:xx
 * RFP (BBa_E1010) with RBS (BBa_B0034) under Ptet promoter (BBa_R0040)
 * 2007:xx:xx


 * iGEM strain box 1
 * 49
 * PompR rfp'
 * DH5α
 * pSB2K3
 * Kan25
 * RFP (BBa_E1010) with RBS (BBa_B0034) under PompR promoter
 * 2007:xx:xx
 * RFP (BBa_E1010) with RBS (BBa_B0034) under PompR promoter
 * 2007:xx:xx


 * iGEM strain box 1
 * 50
 * tetR''
 * DH5α
 * pSB2K3
 * Kan25
 * tetR repressor (BBa_C0040) with RBS (BBa_B0034) and TT (BBa_B0015)
 * 2007:xx:xx
 * tetR repressor (BBa_C0040) with RBS (BBa_B0034) and TT (BBa_B0015)
 * 2007:xx:xx


 * iGEM strain box 1
 * 51
 * DH5α
 * pSB2K3
 * Kan25
 * pSB2K3
 * Kan25
 * Kan25


 * iGEM strain box 1
 * 52
 * lasI''
 * DH5α
 * pSB2K3
 * Kan25
 * lasI (BBa_C0078) with RBS (BBa_E0034) and TT (BBa_B0015)
 * 2007:xx:xx
 * lasI (BBa_C0078) with RBS (BBa_E0034) and TT (BBa_B0015)
 * 2007:xx:xx


 * iGEM strain box 1
 * 53
 * tetR''
 * DH5α
 * pSB2K3
 * Kan25
 * tetR repressor (BBa_C0040) with RBS (BBa_B0034) and TT (BBa_B0015)
 * 2007:xx:xx
 * tetR repressor (BBa_C0040) with RBS (BBa_B0034) and TT (BBa_B0015)
 * 2007:xx:xx


 * iGEM strain box 1
 * 54
 * tetR'
 * DH5α
 * pSB1A2
 * Amp100
 * tetR repressor (BBa_C0040) with RBS (BBa_B0034)
 * 2007:xx:xx
 * tetR repressor (BBa_C0040) with RBS (BBa_B0034)
 * 2007:xx:xx


 * iGEM strain box 1
 * 55
 * lasR''
 * DH5α
 * pSB1A2
 * Amp100
 * lasR (BBa_C0079) with RBS (BBa_B0034) and TT (BBa_B0015)
 * 2007:xx:xx
 * lasR (BBa_C0079) with RBS (BBa_B0034) and TT (BBa_B0015)
 * 2007:xx:xx


 * iGEM strain box 1
 * 56
 * Plac IA7K
 * DH5α
 * pSB1A2
 * Amp100
 * Amp100
 * Amp100


 * iGEM strain box 1
 * 57
 * pSB1A2 attB TG
 * DH5α
 * pSB1A2
 * Amp100
 * Was previously Pr RFP', RFP was cut out and attB TG was inserted. Flanked by EcoRI and PstI sites.
 * 2009:07:06
 * Was previously Pr RFP', RFP was cut out and attB TG was inserted. Flanked by EcoRI and PstI sites.
 * 2009:07:06


 * iGEM strain box 1
 * 58
 * pSB1A2 attB TT
 * DH5α
 * pSB1A2
 * Amp100
 * Same as Strain #57, except the inserted fragment was attB TT instead. This is the wild-type attB.
 * 2009:07:06
 * Same as Strain #57, except the inserted fragment was attB TT instead. This is the wild-type attB.
 * 2009:07:06


 * iGEM strain box 1
 * 59
 * pSB1A2 attB CT
 * DH5α
 * pSB1A2
 * Amp100
 * Same as Strain #57, except the inserted fragment was attB CT instead.
 * 2009:07:06
 * Same as Strain #57, except the inserted fragment was attB CT instead.
 * 2009:07:06


 * iGEM strain box 1
 * 60
 * rPlacTT + PlacCI
 * DH5α
 * pSB2K3
 * 2009:06:15
 * 2009:06:15
 * 2009:06:15
 * 2009:06:15
 * 2009:06:15


 * iGEM strain box 1
 * 61
 * 2008 Construct w/o Promoter
 * DH5α
 * pSB1A2
 * Amp100
 * RBS + Pmel + rPlacTT + PlacCI. 2008 project.
 * 2009:07:10
 * RBS + Pmel + rPlacTT + PlacCI. 2008 project.
 * 2009:07:10


 * iGEM strain box 1
 * 62
 * 2008 Constant
 * DH5α
 * Amp100
 * 2009:07:15
 * Amp100
 * 2009:07:15
 * 2009:07:15
 * 2009:07:15


 * iGEM strain box 1
 * 63
 * pSB3C5
 * DB3.1
 * pSB3C5
 * Cm25
 * Low copy # plasmid, no promoter, no RBS, "CCDD" (BBa 15001/15002)?
 * Cm25
 * Low copy # plasmid, no promoter, no RBS, "CCDD" (BBa 15001/15002)?


 * iGEM strain box 1
 * 64
 * BBa
 * pSB3C5
 * pSB3C5
 * pSB3C5


 * iGEM strain box 1
 * 65
 * pKNG101
 * DH5α
 * pKNG101
 * Strep100
 * Plasmid backbone for the pFB9xxx series.
 * 2009:08:07
 * Plasmid backbone for the pFB9xxx series.
 * 2009:08:07


 * iGEM strain box 1
 * 66
 * DH5α λ pir
 * DH5 alpha lambda pir
 * None
 * None
 * DH5α λ pir stock for making competent cells.
 * 2009:07:24
 * DH5α λ pir stock for making competent cells.
 * 2009:07:24


 * iGEM strain box 1
 * 67
 * pFB9007
 * DH5α λ pir
 * pFB9001
 * Strep100
 * Contains H2 region and an attP site. Same as pFB9004 except w/o the truncated SacB gene on the plasmid backbone.
 * 2009:08:05
 * Contains H2 region and an attP site. Same as pFB9004 except w/o the truncated SacB gene on the plasmid backbone.
 * 2009:08:05


 * iGEM strain box 1
 * 68
 * pFB9008
 * DH5α λ pir
 * pFB9007
 * Strep100
 * Same as pFB9007, but it has RFP cloned in upstream of the attP site and the H2 region.
 * 2009:08:05
 * Same as pFB9007, but it has RFP cloned in upstream of the attP site and the H2 region.
 * 2009:08:05


 * iGEM strain box 1
 * 69
 * pFB9009 #2
 * DH5α λ pir
 * pFB9008+H1
 * Strep100/Kan20
 * Same as pFB9008+H2, but it has Kanr and sacB genes cloned in between the H1&H2 regions, directly downstream adjacent to the RFP.
 * 2009:08:28
 * Same as pFB9008+H2, but it has Kanr and sacB genes cloned in between the H1&H2 regions, directly downstream adjacent to the RFP.
 * 2009:08:28


 * iGEM strain box 1
 * 70
 * 70


 * iGEM strain box 1
 * 71
 * pSB4A5
 * DH5α
 * pSB4A5
 * Amp100
 * Low copy number, no promoter, no RBS.
 * 2009:08:28
 * Low copy number, no promoter, no RBS.
 * 2009:08:28


 * iGEM strain box 1
 * 72
 * pSB4C5
 * DB3.1
 * pSB4C5
 * Cm25
 * Low copy number, no promoter, no RBS.
 * 2009:08:28
 * Low copy number, no promoter, no RBS.
 * 2009:08:28


 * iGEM strain box 1
 * 73
 * OriT-L #13
 * DH5α
 * pSB1A2
 * Amp100
 * 2009:08:28
 * Amp100
 * 2009:08:28
 * 2009:08:28


 * iGEM strain box 1
 * 74
 * OriT-L #18
 * DH5α
 * pSB1A2
 * Amp100
 * 2009:08:28
 * Amp100
 * 2009:08:28
 * 2009:08:28


 * iGEM strain box 1
 * 75
 * BBa_J23116 Pconst
 * DH5α
 * BBa_J61002
 * Amp100
 * 2009:08:28
 * Amp100
 * 2009:08:28
 * 2009:08:28


 * iGEM strain box 1
 * 76
 * BBa_J23118 Pconst
 * DH5α
 * BBa_J61002
 * Amp100
 * 2009:08:28
 * Amp100
 * 2009:08:28
 * 2009:08:28


 * iGEM strain box 1
 * 77
 * pFB9008+H1 #13
 * DH5α λ pir
 * pFB9007
 * Strep100/Kan20
 * Same as pFB9008, except that H1 region and its attP site are cloned in upstream of the RFP gene to flank the RFP. Low copy number.
 * 2009:08:28
 * Same as pFB9008, except that H1 region and its attP site are cloned in upstream of the RFP gene to flank the RFP. Low copy number.
 * 2009:08:28


 * iGEM strain box 1
 * 78
 * pSB4C5-1
 * DB3.1
 * pSB4C5
 * Cm25
 * 2009:08:20
 * Cm25
 * 2009:08:20
 * 2009:08:20


 * iGEM strain box 1
 * 79
 * pSB4C5-2
 * DB3.1
 * pSB4C5
 * Cm25
 * 2009:08:20
 * Cm25
 * 2009:08:20
 * 2009:08:20