Team:Warsaw/Calendar-Main/16 August 2009

Assembly of endosomal detection operon Marcin Task 1: Transformation of chemocompetent E. coli strain TOP10 to reveal the quality of prepared bacteria Comment  The quality of this bacteria is superior to previously prepared ones Task 2: Transformation of chemocompetent E. coli strain TOP10 Constructs to transform:  p53 CDS +  BBa_B0032 on  pSB1A3   <a href="http://partsregistry.org/Part:BBa_C0040"> BBa_C0040</a> + <a href="http://partsregistry.org/Part:BBa_R0080"> BBa_R0080</a> on <a href="http://partsregistry.org/Part:pSB1A3"> pSB1A3</a> </ul> Methods:  Ligation prepared in <a href="http://2009.igem.org/Team:Warsaw/Calendar-Main/15_August_2009">15.08.09</a> was stopped via thermal inactivation in 80&deg;C for 20 minutes</li> Detailed protocol of transformation is described <a href="http://2009.igem.org/Team:Warsaw/Calendar-Main/5_July_2009">here</a>.</li> </ul> Task 3:  Isolate backbone plasmid <a href="http://partsregistry.org/Part:pSB1A3"> pSB1A3</a> </li></ul> Methods:  Plasmids were isolated using the A&A plasmid mini kit. Detailed procedure of the isolation is described <a href="http://aabiot.com/products/dna_purification/plasmid_dna/plasmid_mini/protocol_plasmid_mini.pdf" here>here</a></li></ul>