Team:Todai-Tokyo/Protocols/Restriction Enzyme Digest

Restriction Enzyme Digest Protocol
1. Mix the following in a eppendorf tube:


 * Xµl　plasmid DNA （to a final concentration of 300ng/30ul）
 * 3µl　10 H　buffer
 * 1.5µl　Restriction Enzyme 1
 * (1.5µl　Restriction Enzyme 2, if applicable)
 * MilliQ　up　to　30ul

2. Incubate at 37ºC for 1h or more