Wisconsin-Madison/28 September 2009

September 28, 2009
set up assay to compare and contrast growth in Salt Free LB and Minimal Media, also observe effects of placing GsSDMT on pBAD18

200uL total:

.2 amp

20uL cells grown to .4 OD 600

either:

180uL media

164uL media 16uL 5M NaCl

or

156uL media 24uL 5M NaCl

.2uL DMG

.15uL 15% arabinose

double transformation of 33/GSMT and 18/GsSDMT

developed assay for testing both genes

Digestion of cloning, pBAD35, photopromoter, MetK+Pbad35