Wisconsin-Madison/19 June 2009

June 19, 2009
Ex 14: Ran gel of Miniprep DNA – no digestion

Gel was very thin for some reason. Probably wrong amount of TAE

We have something: 1) GFP1 2) GFP2 3) Trip1 4) Trip2 5) 1k Ladder 6) SDMT1 7) SDMT2

(GEL PICTURE)

Ex 13: Redigestion of Choline, Pro U, and YhfR with different EcoRI (problems reported with Brian’s Enzyme and we just received our own)

Plate Results: Platting of final transformed cells after cloning show no colonies on (F, C, and Y). Pro U (upper right) shows one red colony. Either our digestion or ligation did not work.

Digestion and Ligation of Second half of Phussion PCR Product:

Ex 15: Inducing various Modified (Triple) E.Coli Strain Cultures 3

Made liquid Cultures of E.Coli Strains;

1.	BL21 (DE3)

2.	K-12 MG1655 AaraBAD

3.	K-12 MG1655 (wildtype)

4.	DH10B