Duke University/22 June 2009

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June 22, 2009
LB Agar Plates with Chlorophenicol (CAM), anhydrotetracycline, Nile Red (NR) stock plate CAM 50 mg/ul 20 ug/ml anhyd 2 mg/ml 150 ng/ml NR 0.25 mg/ml 0.5 ug/ml


 * E: PCR with fragments 1 and 2 and different conditions (decreased annealing and extension times and number of cycles), Gel to check PCR

2x Phusion mix 12.5 ul pASKphaA 4.76 ul phaB 0.60 ul H2O 7.14 ul 25 ul

98°C 30s 1x 98°C 10s 70-55°C 10s 72°C 1m 10s 20x 72°C 5m 4°C infinity 1x


 * R: No band/smear
 * C: Change conditions