Team:Brown/Notebook Meetings/6-3-09

Brown iGEM Meeting

June 2, 2009

WebEx Conference Call


 * Attendees: Steph, Will, Indu, Eli, Michael


 * 8:20: Steph will be researching S. Aureus expression vectors, and the natural levels of Staph in the nasal colony
 * 8:25: We are looking into nanoparticle repressor system for Safe Cell
 * 8:31: For Histamine – We are going to focus on making a bifunctional protein – EV131 and IGE antibody binding protein
 * The protein will be made by a Staph strain endogenous to the nasal colony
 * We are targeting specifically the symptoms of Hay fever
 * Secretion and uptake into the bloodstream needs to be researched.
 * 8:36: We should look at past iGEM projects to see if any other teams have done projects with similar aspects as our current 2
 * Have any teams worked with S. epidermidis, or any gram positive bacteria?
 * 8:42: Since S. aureus might be dangerous to work with in the lab, we can do proof of concept with another well-studied gram positive bacteria
 * 8:44: Indu will put up the Histamine step by step project design
 * 8:47: For Safe Cell, we need to research the additional novel kill switch for the 3rd way to kill a cell.
 * We also need to find a solid repressor system.
 * 8:55: S. epidermidis is endogenous to the nose!
 * Can we order colonies of S. epidermidis? Eli is looking at that.
 * 9:00: Will is researching everything about S. epidermidis
 * Eli is looking into secretion / uptake
 * Indu and Michael are looking into creating a bifunctional proteins
 * 9:02: Flora, can you start looking up nanoparticles in a repressor system?
 * Ahmad, we need you to look into a 3rd killing mechanism for the cell.
 * Everyone should start fleshing out potential project designs.
 * 9:04: Indu will be making a list of “materials” we need
 * Can we get Histamine plates? Or IGE Antibody plates? Where can we get these?
 * How can we test binding affinity? We will having our binding proteins on a stationary phase. Wash over with fluorescent protein. (?)
 * 9:11: As you can see there are a lot of things to be researched! There is a new sign up sheet on the Google group.
 * 9:14: Next meeting is Monday 6PM Pacific Time.