Team:UNICAMP-Brazil/Notebooks/August 28

Kluyveromices lactis genes

 * The PCR for the K. lactis genes worked very well! =] We got specific bands for the 3 sequences (agarose gel above).

Terminator biobrick
The colony PCR was made to confirm the presence of terminator transformants, because the antibiotic resistence is not working to any antibiotics.
 * Colony PCR



We plated the cells in LB and picked up some colonies with a toothpick, inoculated on a backup LB plate and put the toothpick on a tube with 20µl H2O. Then, denatured these cells at 95°C during 5 minutes and heat shocked on ice for 5 minutes. Spinned the tubes and used 1 µl to make the PCR.

We also inoculated 10 colonies transformed with the terminator biobrick to perform the miniprep tomorrow.
 * Preparing for Miniprep

Wesley