Team:Kyoto/GSDD/Notebook/0914-0918



 /* ul#navi_GSDD or ul#navi_CiC */ /* li.nav_i, li.nav_me, li.nav_g, li.nav_r, li.nav_p, li.nav_mo, li.nav_n */

background-position:left bottom !important; color:black; }
 * 1) navigation ul#navi_GSDD li ul li.nav_n a{
 * 1) Home
 * 2) GSDD
 * 3) Notebook

To Do

 * p-2-(1)(4)(7)(8)(10)
 * Miniprep
 * Restriction Enzyme Digestion
 * p-2-(1)(4) E.X
 * PCR purification kit
 * p-2-(1)(7)(8)(10) E.S
 * Gel extraction
 * 4-(1)(2)(3)
 * Colony PCR
 * Insert check

p-2-(1)(7)(8)(10) E.S

 * Gel extraction kit

4-(1)(2)(3)

 * This result indicates that (1)a, (2)b and (3)a were inserted properly.

To Do
Further to 14 Sept.


 * p-2-(1)(4)(7)(8)(10)
 * Miniprep
 * Restriction Enzyme Digestion
 * p-2-(1)(4) E.X
 * PCR purification kit
 * p-2-(1)(7)(8)(10) E.S
 * Gel extraction
 * 4-(1)(2)(3)
 * Colony PCR
 * Insert check

To Do

 * ligation
 * transformation

Transformation
No colony was observed (090917 10:00)

To Do

 * Make repetitive sequence (MPR)
 * Transformation(retry)