Team:Warsaw/Calendar-Main/29 July 2009

Cloning of the mgtc promoter into the pSB1A3 plasmid Kamil

Tasks:  Bacteria transformation 

Methods:  A 200&mu;l batch o chemocompetent bacteria was transformed according to the protocol with 15&mu;l of ligation mix. 1x and 10x (100&mu;l each) concentrations of bacteria were incubated on Petri dishes containing LB broth and ampicilin. The bacteria were incubated overnight at 37&deg;C. 

Results:  Both dishes yielded considerable amounts of white colonies (pSB1A3 has a white/red selection)

Conclusions:  The ligation seems to be successful.</li> </ul>

Construction of <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K177012 ">K177012</a>  operon1_part2

Ania Tasks:  Digest the only only one promising clone obtained with SchI and PstI to confirm the sequence</li> </ul> Results: <img src="http://2009.igem.org/wiki/images/e/e0/Jarek_Ania_290709.jpg"> Comments: The obtained fragments are of the wrong size. The clone is not correct.