August/7 October 2009

1. colony check sample no. no. of colony 11                  +++    20                   +++    21                   +++   2-6O                 ++ 1-15J               + 1-15L               +

2.transformation sample no. 67, 68 , 69 , 52 , 11 , 20 , 21 , 2-6O , 1-15J , 1-15L

3.digation

K204070

K204071

K204072

K204073

K204074

K204075

↓ 37 degree. O/N

4. min prep sample           conc. X4                  257 ng/uL X4                  266 X4                  171 X5                  169 X6                  293 X7                  294 X8                  151 59                  306  61                   285  64                   252  65                   278

5.digation

X4

↓ 37 degree, 2hr gel cut

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