Team:UC Davis/wiring pH sensor

             <span style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"> </b><b style="color: rgb(255, 255, 153);"><span style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">   <img alt="" src="http://2009.igem.org/wiki/images/b/b9/UCDAVIS_PIC8.png" style="border: 0px solid ; width: 78px; height: 36px;"></a>   </b><b style="color: rgb(255, 255, 153);"><span style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">   <img alt="" src="http://2009.igem.org/wiki/images/2/2f/UCDAVIS_PIC5.png" style="border: 0px solid ; width: 81px; height: 36px;"></a>   </b><b style="color: rgb(255, 255, 153);"><span style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"> </b><b style="color: rgb(255, 255, 153);"><span style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">   <img alt="" src="http://2009.igem.org/wiki/images/a/a6/UCDAVIS_PIC6.png" style="border: 0px solid ; width: 78px; height: 37px;"></a> <img alt="" src="http://2009.igem.org/wiki/images/1/1d/UCDAVIS_PIC7.png" style="border: 0px solid ; width: 83px; height: 37px;"></a>   </b><b style="color: rgb(0, 0, 0);"><span style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"> </b> <hr style="width: 100%; height: 2px;"> <span style="font-weight: bold; text-decoration: underline;">Wiring the pH sensor into our system: Th pH sensor: ChvG/ChvI is a two-component system and a suspected pH sensor. ChvG is the histidine kinase and ChvI is its cognate response regulator(2). Together they are believed to sense extracellular pH between 5.5 to 7 and relay this signal to regulate the expression of key virulence-related genes(2). In our design, we used this two-component system from <i>Agrobacterium tumefaciens</i> to regulate the expression of select target genes. <hr style="width: 100%; height: 2px;"> <span style="text-decoration: underline; font-weight: bold;">Possibility of Cross-Talk: Bacteria have numerous related two-component systems based on kinase/response-regulation(5). For instance, the A. tumefaciens genome is known to contain at least 25 two-component pathways (2). Therefore, there are possibilities of cross-talk between some genes present in E. coli that have the same mechanism as the ChvI/ChvG system. In fact, there is evidence supporting that ChvI can complement a PhoB deletion in E. coli. (3) Therefore it is highly likely that there is a possibility of cross-talk between our new sensory systems elements, present in E. coli. Many protein-protein interactions are not fully understood (5), which makes this project more challenging but very interesting. <hr style="width: 100%; height: 2px;"> <img style="width: 809px; height: 464px;" alt="" src="http://2009.igem.org/wiki/images/e/ea/UCDAVIS_pH_sensor1.jpg"> Click on the specific part or interaction for more information. Parts: ChvG</a>, ChvI</a>, PhoR</a>, PhoB</a> Interactions: ChvG---&gt; ChvI</a>, PhoR---&gt; ChvI </a>, PhoR---&gt;PhoB</a> ,ChvI---&gt;PhoA </a> <p class="MsoNormal" style="background: white none repeat scroll 0% 50%; text-indent: 0.5in; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial;"><span style="font-size: 14pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;"><o:p></o:p> </b>