ULB/7 September 2009



 iGEM Team:ULB-Brussels Wiki  

  /* Design for http://2009.igem.org/Team:ULB-Brussels

Université Libre de Bruxelles

html body { width: 910px; font-family: Arial; margin: 0 auto;

}

html body div.header { margin: 0px; padding: 0px; margin-top: -21px; /* strange... */ }

/* ===== banner ===== */

/* banner = large image on the top of the page, begind the title */

html body div.header div.banner { height: 171px; background-image: url(http://2009.igem.org/wiki/images/0/0a/Logoulb1.png); background-repeat: no-repeat; }

html body div.header div.banner h1 { padding-top: 50px; text-align: center; text-decoration: none; border: none;

color: #4E7DB7; font-size: 40px; }

html body div.header ul { /* these are common to both 'menu' and 'submenu' */ margin-top: 0px; margin-bottom: 0px; list-style-type: none; list-style-image: none; }

/* ===== menu ===== */

/* menu = horizontal bar containing the main links to other sections of the wiki */

html body div.header div.menu { background-color: #3E6DA4; color: white; height: 30px; padding: 0px; }

html body div.header div.menu li { float: left; text-align: center; width: 120px; margin: 0px; margin-top: 10px; } html body div.header div.menu li :hover { color: #5F7EB4; background-color: #FFFFFF;

margin: 0px;

padding-left: 15px; padding-right: 15px; padding-top: 4px; padding-bottom: 3px; }

/* class 'selection' refers to the 'selected' menu, in order to change it's color to white */ html body div.header div.menu li a.selection { color: #5F7EB4; background-color: #FFFFFF;

margin: 0px;

padding-left: 15px; padding-right: 15px; padding-top: 4px; padding-bottom: 3px; }

html body div.header div.menu li a { color: white; text-decoration: none; }

/* ===== submenu ===== */

/* submenu = changing horizontal bar containing the main links to subsections of other sections of the wiki */

html body div.header div.submenu { /*background-color: #404040;*/ background-color: white; height: 25px; font-size: small; border-bottom-style: solid; border-bottom-width: 1px; border-bottom-color: #AAAAAA; }

html body div.header div.submenu li { float: left; width: 120px; text-align: center;

margin: 0px; margin-top: 4px; }

html body div.header div.submenu li :hover { color: #5F7EB4; background-color: #FFFFFF;

margin: 0px;

padding-left: 15px; padding-right: 15px; padding-bottom: 3px; }

html body div.header div.submenu li a { color: #5F7EB4; text-decoration: none; }

/* ===== panel ===== */

/* panel = box containing mascotte, contdown and links to sponsors */

html body div.panel { float: right; border-left-style: solid; border-left-width: 1px; border-left-color: #AAAAAA; padding-left: 10px; margin-left: 10px; margin-top: 20px; width: 250px; font-size: small; }

/* appearance of the links */ html body div.panel a { text-decoration: none; font-weight: bold; color: #3E6DA4; }

html body div.panel a:hover { color: #F5B455; }

/* == countdown == */

html body div.panel div.countdown { /*margin-top: 20px; margin-bottom: 20px; height: 30px;*/ margin: 20px; padding: 20px; }

.countdown font { padding: 20px; margin: 20px; color: #5F7EB4; }

/* == sponsors == */

html body div.panel div.sponsor { margin-bottom: 10px; min-height: 50px; }

/* appearance of the sponsor's logos */ html body div.panel div.sponsor img { width: 50px; height: 50px; border-style: none; float: left; margin-right: 7px; }

/* ===== main ===== */

html body div.main { font-size: small; padding-left: 10px; padding-right: 10px; width: 910; text-align: justify; }

html body div.main p.abstract { font-weight: bold; }

html body div.main div.welcome img { width: 626px; height: 150px; margin-top: 10px; border-style: none; }

html body div.footer { }

 Home Team Project</a></li> Parts</a></li> Safety</a></li> Notebook</a></li> Sponsors</a></li> </ul> <ul>

</ul>

<style type="text/css">

table.calendar         { margin: 0; padding: 10px; } table.calendar td      { margin: 0; padding: 2px; vertical-align: top; } table.month .heading td { padding:2px; background-color:#3E6DA4; color:#FFFFFF; text-align:center; font-size:120%; font-weight:bold; } table.month .dow td    { color:#aaa; text-align:center; font-size:110%; } table.month td.today   { background-color:#ddd; } table.month td { border: none; margin: 0; padding: 1pt 1.5pt; font-weight: bold; font-size: 12pt; text-align: right; background-color: #eee; } .day-active { color:blue } .day-empty { color:grey }
 * 1) bodyContent table.month a { background:none; padding:0 }

{| align="right"

Results of ligation:


 * Colonies of ligation with IPTG present a filamentous appearance when we spade with a toothpick.


 * Phenotypic tests:


 * Spreading bacteria between a glass surface and a sheet of paper: we can feel resistance.

Extracting of plasmides 9, 10, 5, 6 and 2.
 * Comparaison of little balls of spreading bacteria from sticking with those from another transformation: the balls of sticky bacteria have pasted and blanck balls didn't paste.

Transformation of 5 ligations carried out on 9/5.

Dubbing of the ligation 1 with IPTG in IPTG medium.

Spreading of a large quantity of ligation 1 and 2. Ligation of promoter-RBS-RFP and hfsG-hFSH-terminator.
 * Results:No fluorescence

Following ligations:
 * 1) LIG1: Tube 4 (insert) + Tube 5
 * 2) Lig2: Tube 5 (insert) + Tube 9