Template:Team:KULeuven/10 October 2009/VanillinProduction

for 50 mg/&mu;l Ferrulic acid for 5 mg/&mu;l Ferrulic acid for 50 mg/&mu;l Tyrosine for 5 mg/&mu;l Tyrosine
 * Vanilla experiment take 2
 * From the grown cultures (EFT, PEFT, PAMSCOT, SAMS) transfer 1:100 to a new LB medium
 * Add 50 mg/L Tyrosine to 1 falcon and 5 mg/L to another test tube
 * Let the PEFT and EFT cells grow for 2 hours, then add 50 mg/L Ferrulic acid and 5 mg/L ferrulic acid
 * Note: Ferrulic accid is dissolved in alcohol, 10 mg/ml
 * Take samples for PAMSCOT and SAMS at: 0, 0', 30, 60, 120, 180, 240, 300 and 360 minutes
 * Take samples for PEFT and EFT (after 2 hours growth) at: 0, 0', 20, 40, 60, 90, 120, 180 and 240 minutes
 * Measure the OD at 595 nm