ULB/22 July 2009



 iGEM Team:ULB-Brussels Wiki  

  /* Design for http://2009.igem.org/Team:ULB-Brussels

Université Libre de Bruxelles

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 Home Team Project</a></li> Parts</a></li> Safety</a></li> Notebook</a></li> Sponsors</a></li> </ul> <ul>

</ul>

<style type="text/css">

table.calendar         { margin: 0; padding: 10px; } table.calendar td      { margin: 0; padding: 2px; vertical-align: top; } table.month .heading td { padding:2px; background-color:#3E6DA4; color:#FFFFFF; text-align:center; font-size:120%; font-weight:bold; } table.month .dow td    { color:#aaa; text-align:center; font-size:110%; } table.month td.today   { background-color:#ddd; } table.month td { border: none; margin: 0; padding: 1pt 1.5pt; font-weight: bold; font-size: 12pt; text-align: right; background-color: #eee; } .day-active { color:blue } .day-empty { color:grey }
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{| align="right"

Amplification
PCR on:
 * hfsG gene
 * hfsH gene
 * promotor
 * terminator
 * RBS + RFP

PCR cycles
3 different PCR cycles have been carried out :
 * at 55°C
 * at 60°C
 * a special « deletion programme» (for longer primers)

Running on a agarose gel (55° and 60° programs)

Results

 * biobricks : ok
 * hfsG and hfsH gene: same results as previously