Illinois/10 July 2009

July 10
sRNA Library

Hopefully now the ligation will be working. After many many attempts we got a decent looking gel! The resolution isnt very good. This was an attempt to fit both the insert and the vector on the same gel but that didn't work at all. Its 1% with a 1kbp ladder.



The large, slow bands are hopefully the ligated plasmids. The ones just below that don't look like they worked. They look reasonably bright. Each lane is a variant of Insert:Vector ratio, ranging from 3:1 to 15:1 which were all ligated at room temperature for 4 hrs. (Protocol called for 1hr)

3'-Acting sRNAs

Today we performed a midiprep to extract the plasmid DNA from dH5α.

Hybrid Promoter

Today we came up with a schematic of a possible decoder:

We looked more closely at the biobricks: BBa_I14032, BBa_R0040, BBa_K137125, BBa_K091101.

BBa_R0040 and BBa_K091101 were available to us in the registry distribution so we transformed these into cells to test them.

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