Team:TorontoMaRSDiscovery/31 August 2009

=August 31, 2009=
 * 1) Stocked 4+5R culture (retransfection)
 * 2) Miniprepped overnight cultures of 4+5 (3,4,5,R), BB7, and EncX,Y,Z
 * 3) Digested EncC with E,S; 5 with X,P; Tet, 7, EncX,Y,Z with E,P
 * 4) Ran digests on a gel
 * 5) *EncY shows Enc insert
 * 6) *5 digest lane shows a thick band the same size of the linear isoform in the control
 * 7) Poured C plates
 * 8) 3+2 tranformation from Aug 27 showed a few colonies
 * 9) 3 largest colonies were picked to start overnight culture
 * 10) Started overnight ligation of Enc+5/Tet with negative control