Team:UAB-Barcelona/Primersp

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These were the primers we ordered for the phosphate:

•	phoA: 		EcoR1-Not-Xba— phoA – Spe

Reverse: S    5’ GCTCATA ACTAGT TTTATTTTCTCCATGTACAAATACATTAAAA 3’ Annealing temperature = 74 ºC

Forward: E     N       X      5’ AGATTCA GAATTC GCGGCCGC TCTAGA GTTTCAACAGCTGTCATAAAGTTGTCAC  3’   Annealing temperature= 74 ºC