Team:Newcastle/Meetings/8 October 2009

= 8 October 2009 - iGEM Meeting =
 * Chair: Mat
 * Students: Jess, Jane, James, Hanny, Goksel, Mat, Arun
 * Minutes: James
 * Instructors: Jen, Neil
 * Advisors: Dan, Morgan
 * Guests: Zoltan

Agenda

 * General News
 * Review last week's general action points
 * Review last week's specialist action points
 * Give summaries in the following areas:
 * Lab Work (Goksel and Hanny)
 * Wiki (Mat)
 * Presentation (Arun)
 * Poster (Jess)
 * Modelling (James)
 * T-shirt/Marketing (Jess)
 * Questions

Last Week's General Action Points

 * Design sequencing primers for pMutin4 and GFP-rrnB (Arun)
 * Matt Pocock needs to fill in ESTA form
 * Create a page on the wiki about ethics (Mathew)
 * Fill Valencia's questionnaire (everyone)
 * Get Hanny guest student card
 * Search for other websites for those green "bugs" (Jess)
 * Contact Nigel Robinson to use his lab and the electronmicroscope
 * Citate the papers in the wiki for metal sensing. Quote short sentences where necessary.
 * Goksel to send DNA off to Parts Registry.

Lab

 * Hanny to sequence BioBricks

Wiki

 * Need to update lab work section
 * Need to study Imperial 2008 iGEM wiki's video for ideas.
 * Redraw and add colour to diagram on front page currently (the process chart) - possibly using Omnigraffle for Mac
 * The "Eco Warrior" story, which is currently on the front page of the wiki, needs to go in the 'Fun and Games' section.
 * Put a diagram of a Bacillus subtilis' cell on the front page of the wiki with elements of the project e.g. Cadmium sensing, stochastic switch, etc included
 * Make a date for Sunday 11th October for students meetup to take photos around the university/city.

Presentation

 * Sub-project teams need to send at least two slides with some information on them.
 * Presentation practice to be held on 23rd October - times to be decided

Poster

 * Jess needs to update team by next week
 * Each team to create document for the poster creation.

Modelling

 * Make a date for a Modelling meeting to work out parameter values, and how to integrate into population model.

T-shirt/Marketing

 * Get t-shirt designs to printing company

Minutes

 * Introduced the team to Zoltan

Labwork

 * Hanny - kinA worked, now in E.coli, transforming into B.subtilis - need to make sure it has gone into amyE - plating out onto starch and IPGT plates looking for spores and halos - if done, characterised!
 * Goksel - cotC, re-PCRed, re-cut into pMutin4 - should work now.
 * Stocastic Switch still stuck at 85% complete.
 * Metal Sensor also stuck at 85% complete.
 * Safety Officer visted the lab - Arun, Mat and Hanny were not wearing lab coats - Mat and Arun should not have been there.
 * Goksel is to go on some safety courses, if he is to be in the lab in the future.
 * Sequence Biobricks - not done yet - tell Phil that we will pay.
 * Request for variance still pending.
 * May need to clone kinA into Biobrick compatible vector.

On last weeks APs

 * For pMutin4 sequencing primers, just use pMutin2 sequence.
 * Dan emailed Matt Pocock to check if he has done the ESTA.
 * Mat still needs to do the ethics page.
 * Goksel and Jess still need to do Valencia's questionairre.
 * Hanny still needs a guest card.
 * Could make our own green bugs
 * Nigel Robinson replied - need to ask him about other metals.
 * Need to cite papers.
 * Prepare DNA for submission - watch for postal strike.

Wiki

 * Lab Rota to be removed. (James - done)
 * Duplicate project overview to be removed. (James - done)
 * Header photo to be changed.
 * Add images/text for Next and Previous.
 * Reviewed animations - liked, and using.
 * Need to change format of Matt's video, and upload. (James)
 * All teams need to update lab book.
 * Photo date set for Saturday 10th at 10:00am.

Presentation

 * Practice Presentation with the Bioinformatics group is set for Friday 23rd October at 1-2pm.
 * Still doing a presentation in Sunderland?
 * Use Sir Bac Man as first slide.
 * Explain the life cycle and how we are changing it.
 * Arun has prepared a document.
 * Jen and Neil are away in Spain 17-21 October.
 * Each team is to send Arun more detail.

Poster

 * Jen in to send links to designs.
 * Jess' draft has too much small text - more images and talking!
 * Need to get back to sponsors to show them what we have achieved.
 * Could use a complex wheel to point to various points of the poster.
 * Each team is to send Jess more detail.

Modelling

 * Send a list of parameters for the population model (James - done)
 * Inspect G.g.n's code for online model (James)
 * Arrange a modelling meeting - Monday (internal) (James)

T-shirts

 * Due to the situation - reduce the number to about 15, and increase the price £20-30
 * Could get it partially done by professionals, then iron on bits - small logos.
 * Could use white shirts.
 * Could use other companies.