August/8 August 2009

Today we carried out the following procedures (in rough chronological order):

1. Checked the cell cultures transformed and plated out yesterday (8/7) for colony formation and made an approximate count of the number of colonies.

(plate number)-(location on plate) (no. of colonies) ---            2-12H                        none 1-12C                       none 1-12A                       none 2-24G                       none 2-18F                       none 1-6O                        ~10 1-10K                       ~10 1-18L                       ~10

2. Conducted a 'miniprep' to harvest and check concentration of plasmids from 10 sample. (incubated since yesterday 8/7~) Result of Nanodrop concentration check:

(sample No.) (concentration) [260/280] [260/230] ---    B0015(1-23L)           24.9 ng/uL     1.77      1.77 K118002(2-16F)      36.0 ng/uL     1.77      1.99 I0462(1-8O)           19.3 ng/uL     1.65      1.59 B0034(1-2M)           18.4 ng/uL     1.64      2.01 I742158(1-19B)      40.9 ng/uL     1.56      0.83 K118003(2-16H)      27.7 ng/uL     1.68      1.38 C0076(1-14D)           9.3 ng/uL     1.66      2.31 C0179(2-8M)            9.9 ng/uL     1.65      1.90 S03878(2-16M)        24.6 ng/uL     1.72      1.24 C0077(1-14F)           2.5 ng/uL     1.11      0.25   failure? I1466(1-23J)           failure

3. gel electrophoresis

↓ 37°C,22hr ↓ failure　(SYBR Safe : 30min???)

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