EPF-Lausanne/12 August 2009

  

12 August 2009

Wet Lab
The plates from yesterday's transformation had A LOT of clones so we did a colony PCR of about 15 clones per plate to check the efficiency of the ligation.

Redid a normal PCR on the 1.5-step PCR products since concentrations weren't high enough.

Prepared LB-Agar plates with ampicillin and kanamycin antibiotics.

Prepared a tetracycline stock (to use as an inducer). Cells were left to grow in LB medium with different concentrations of this stock at 37°C as a test for their growth speed: OD checked every hour.

We did a ligation of our read out system 2 during 1h and we did a transformation with the obtained products. Plates were left overnight at 37°C.

People in the lab
Nath, Basile, Gab, Christian, Nicolas

 