Template:Team:KULeuven/14 September 2009/BlueLightReceptor


 * 1) miniprep of ligY colonies. nannodrop: 111 ng/µl and 116 ng/µl.
 * 2) restriction digest of ligY miniprep
 * 3) gelelectrophoresis of restricted ligY and compared to ligA. the ligY fragment was larger than ligA which means that the ligation Y fauled.  the promotor region probably ligated to RFP which was not restricted out of the vector properly.
 * 4) gel extraction of plasmid pSB1A2 (nanodrop: 10ng/µl)
 * 5) a new ligY was performed
 * 6) control of pcr-product . It was ok.