Team:Brown/Notebook Meetings/8-17-09

iGEM Meeting

8-17-09

SFH 218

9 AM


 * Team 1 update:
 * Today they are making the Blotto solution with 6-His
 * Purchased Qiagen nickel column purification kit
 * After protein comes down in a nickel column
 * Tests for histamine binding; selective binding?


 * Possible ideas for after binding test; Will suggests Barnea lab competitive binding assay involving GPCR => fluorescence
 * Adrian suggestion: Look at crystal structure, and binding pocket; see if you can cut the rest of the protein off and make minimized histamine binding domain
 * Degradation mechanism?


 * Team 2 update:
 * “Chugging right along” all this before Thursday
 * Signal peptide construct finished in expression vector for S. epi
 * Agr promoter construct in S. epi
 * Checking Trg-EnvZ ligations tonight
 * S. epi is resistance to Tet
 * Looger sent us designs to modify Taz to a histidine receptor; then will move to histamine receptor


 * Team 3 update:
 * Mutagenic PCR….
 * Administrative
 * Bio 1950 - Fill out proposal form on bio website
 * Planning in advance
 * Practice presentation in front of people.
 * Google calendar? Weekly lab meetings
 * Wiki has to be done a week before the week its due
 * Cleaner lab book
 * Clean wiki


 * Mutagenic PCR