Team:UNIPV-Pavia/Rebuilt

The last image reports the inverter behaviour of tetR QPI component in LB medium. Considering RPU of promoter regulating tetR expression as input and RPU of tetR QPI device as output it is possible to characterize the logic inverter reported in figure. NB: RPU of promoters BBa_J23100 and BBa_J23118 have been calculated as reported in the dedicated section, while the absence of promoter has been associated to RPU=0. When input (RPU of the promoter expressing tetR) is high, the output is low and the behavior is opposite for high input RPU.

Conclusions
The expected behaviour of this part was not to be affected by the presence of inducer, because the repressor should not be present. In fact, tetR production isn't regulated by any promoter and Ptet can work as a constitutive one.

Actually, the activity of promoter is not independent from aTc concentration, probably because of spurious transcription of tetR repressor in the high copy number plasmid. The experiments concerning this part have been performed in M9 supplemented medium. In LB medium the component has been tested only in absence of inducer and this has been useful to estimate three points of the transfer function of BBa_K173005 as a logic inverter.

We can say that the behaviour of BBa_K173005 as a logic inverter has been validated, even if more points of the transfer function have to be measured.

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