Uppsala-Sweden/19 September 2009

Transformation evaluation
Crappy transformations for some reason, only three colonies for the pUC19 control. Good transformation for the PRYT construct.

Re transformation of R-KRYT, pPetE-RPRA, PRYT and PpetE.

Transformation protocol evaluation
Due to bad transformations we decided to evaluate the protocol, experimental table:




 * 1) 3 wins hands down, but hard to tell if the real cause is batch variance.... Result still a far cry from the 80+ colonies recorded in the initial trial, degraded plasmid?

--Anders 21:16, 19 October 2009 (UTC)