September/22 September 2009

1. colony check sample no  no. of colony 36             5    37              0    38              1    29              0

2.min prep sample no      conc. 3-7G         94.8 ng/uL 2-3I         114 1-8K         144 2-18F        120 136  46            77   2-9A          132 3-7C         149 27B          104 29A          7.9 46           65

3.transfromation sample no. X1-A, X2-A , X3-A 1-12M

4.digestion

K204028

K204030

↓ 37°C, 2hr ↓ gel electrophoresis gel cut purification ↓ ligation O/N

5.transformation sample no.  22, 25 , 40 , 47

back to NOTES