Wisconsin-Madison/2 June 2009

June 2, 2009
Other: Floors in Lab have new finish… No more leaking Autoclave juices pouring on the summer lab in the basement

Ex 6:	Inducible Cultures

Inoculated four 250 mL beakers of 100 mL salt-free liquid LB

Samp1: 100uL, amp 100uL Cm, 73.5 uL Tet : Triple

Samp2: 100uL, amp 100uL Cm, 73.5 uL Tet : Triple

Samp3: 100uL, amp 100uL Cm, 73.5 uL Tet : Triple

Samp4: 100uL, amp 100uL Cm : Empty Vectors pBad33 and pTrc99

Ex 5: Results from plating of competent cells from previous day:

No freezing produced colonies. Freezing for 1 hour produced no colonies.





Results: Have triple transformation with Combination of interest (MevT, MBI, NudF) For using comp cells: either fresh of frozen overnight

Primer: Designed Primers for NudF

Other:	Worked on Wiki Pages