Team:Southampton/Project/Protocols

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">   University of Southampton Wiki     /*TOOLBAR*/ position:absolute; left:-2px; top:0px; width:978px; height:27px; z-index:100; } /*FOOTERPUSH*/ width:1px; height:1457px; } html, body { margin: 0; padding: 0; } /*LAYOUT BEGINS*/ width: 95%; }	position:absolute; left:0px; top:296px; width:975px; height:1200px; background-image:url('http://www.personal.soton.ac.uk/jeh2v07/images/bg.gif'); background-repeat:repeat-y; margin: 0; padding: 0; color: #5D5D5D; }	position:absolute; left:116px; top:1495px; z-index:150; color: #333; }	position:absolute; left:0px; top:1466px; width:975px; height:61px; z-index:100; }	color: #5D5D5D; }       Home <li class=" cssMenui"> Project <![if gt IE 6]></a><![endif]> <ul class=" cssMenum"> <li class=" cssMenui">Project Aims</a></li> <li class=" cssMenui">Project Introduction</a></li> <li class=" cssMenui">Project Description</a></li> <li class=" cssMenui">Project Achievements</a></li> <li class=" cssMenui">Project Projection</a></li> <li class=" cssMenui">Project Safety</a></li> <li class=" cssMenui">References and Links</a></li> <li class=" cssMenui"> Protocols <![if gt IE 6]></a><![endif]> <ul class=" cssMenum"> <li class=" cssMenui">Protocol Overview</a></li> <li class=" cssMenui">Protocol Theory</a></li> <li class=" cssMenui">Biobrick Transformation</a></li> <li class=" cssMenui">Colony PCR</a></li> <li class=" cssMenui">DNA Double Digestion</a></li> <li class=" cssMenui">Gel Electrophoresis</a></li> <li class=" cssMenui">High Fidelity PCR</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Project/Protocols/LBplates">LB Plates</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Project/Protocols/Ligation">Ligation</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Project/Protocols/Ligationtransformation">Ligation Transformation</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Project/Protocols/PlasmidPrep">Plasmid Preparation</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Project/Protocols/TA">TA Cloning</a></li> </ul> </li> </ul> </li> <li class=" cssMenui"><a class=" cssMenui" href="#">Biobrick Parts<![if gt IE 6]></a><![endif]> <ul class=" cssMenum"> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Parts/GoL">Game of Life</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Parts/RPS">Rock Paper Scissors</a></li> </ul></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Team"> Team <![if gt IE 6]></a><![endif]> <ul class=" cssMenum"> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Team">Team Profiles</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Team/Advisors">Advisor Profiles</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Team/Southampton">Southampton Profile</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Team/University">University Profile</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Team/Gallery">Gallery</a></li> </ul> </li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Modeling"> Modelling <![if gt IE 6]></a><![endif]> <ul class=" cssMenum"> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Modeling">Modelling Overview</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Modeling/GoL">Game Of Life</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Modeling/RPS">Rock Paper Scissors</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Modeling/Open">Open Access</a></li> </ul> </li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/HP"> Human Practices <![if gt IE 6]></a><![endif]> <ul class=" cssMenum"> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/HP">Overview</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/HP/Outreach">Outreach</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/HP/igemexper">iGEM Experience</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/HP/Political">Political Awareness</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/HP/Legacy">Our Legacy</a></li> </ul> </li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Notebook">Notebook</a><![endif]> <ul class=" cssMenum">
 * 1) toolbar {
 * 1) footerpush {
 * 1) notebooktable {
 * 1) maincont{
 * 1) sufooter {
 * 1) contfoot {
 * 1) maincont div #Table_01 {

<li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Notebook">Notebook</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Notebook/Labbook">Lab Book</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Notebook/Advisorblog">Advisor Blog</a></li> </ul> </li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Sponsors"> Acknowledgments <![if gt IE 6]></a><![endif]> <ul class=" cssMenum"> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Sponsors">Sponsors</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Southampton">Team Southampton</a></li> </ul> </li> </ul> <img style="width: 975px; height: 24px;" id="bg" src="http://www.personal.soton.ac.uk/jeh2v07/images/maintop.gif" alt="" /> <img style="width: 63px; height: 63px;" alt="" src="http://www.personal.soton.ac.uk/jeh2v07/images/logobox.jpg" /> Protocol Map As part of the preparation for iGEM 2009, we have peformed a number of experimental processes which are detailed in protocols. We are making these protocols available on our wiki to help future teams in their synthetic approach. These protocols can be accesed together with an explanation of the theoretical basis using the links embedded in the diagram below:

| <a href="#">Top</a> | University of Southampton iGEM 2009 <img src="http://www.personal.soton.ac.uk/jeh2v07/images/mainfoot.gif" width="975" height="61" />