Team:Brown/Notebook Recipes/SOB

Making SOB Medium ( For growing competent E.Coli cells)

•	Making 1L of SOB •	Please look at Pg 1356 of the Lab Reference Book for details. 1.	20g Bacto tryptone 2.	5 g of Bacto yeast Extract 3.	0.584g NaCl 4.	0.186g KCl 5.	1L ddH2O 6.	2.4g MgSO4 (OR MgCl2 in 90ml ddH2O ) ➢	Swirl the flask until the ingredients are completely dissolved. ➢	Autoclave the medium.