Wisconsin-Madison/5 July 2009

July 5, 2009
Ex 16: Cloning

Gel Extraction:

Made 2: 40 mL of 1% Gel – 0.4 g (8 wells)

3 uL Dye / 4 uL Sample

20 uL 1)NudF1, 2)NudF1, 3)NudF2, 4)NudF2, 5) Lac1, 6)1kb Ladder, 7)Lac2, 8)Lac2

Attempted gel extraction but couldn't see the band

Ex 19: Parts from the Registry

Inoculate:

1.	lac

2.	lac+GFP

3.	 RFP

Primer: Design for GsSDMT, new ProU(including ProV), MevT, MetK, pMBI