Team:Newcastle/Meetings/22 May 2009

IGEM Meeting 22 May 2009
Attendees: Neil Wipat, Hennifer Hallinan, Goksel Misirli, Hang Zhao

Action Points

 * We want to find an answer to whether SigmaA is expressedin late sporulation stages. To find the answer we need to use microarray datasets specific for sporulation.
 * Assign PoPS for different SigmaA promoter using the microarray data. Hence we will have different sigmaA promoterw with different strength. It is difficult to say the promoter strength from a DNA sequence but microarray data can be used to assign relative PoPS then this criteria can be used to rank the promoters based on their strenghts.
 * Build a promoter libray by changing the flanking sites, by changing the consensus sequences
 * Add a column to the microarray data and display which sequences of sigmaA is being used.
 * Take the alignment and order from the strongest to the weakest
 * Use microarray data to define the consensus sequence for sigmaA
 * Survey of DNA synthesis.