Team:Groningen/Notebook/24 September 2009

Team groningen giving a talk at the Kluyver Centre.

The Management Team of the Kluyver Centre has decided to offer the opportunity to all Dutch iGEM -teams 2009 to present their idea during the 2nd Programme Day of the Kluyver Centre. This event will take place at the Wageningse Berg on the 24th of September. Every team will have 10 minutes to present in front of the scientific community of the Kluyver Centre. The first presentation will start at around 14.15.   14.00-15.15: Plenary session
 * KC announcements (20 min)
 * iGEM VUA (10 min)
 * iGEM Delft (10 min)
 * iGEM RUG (10 min)
 * Ethics and Synthetic Biology (25 min)

Metal Accumulation

 * Grow E.coli for As(III) uptake experiment
 * Grow 1L TB medium with E.coli WT (pSB1A2 + pLac), E.coli + pArsR-RFP
 * Grow 0.5L TB medium E.coli + pLac-fMT
 * Grow @ 30&deg;C and 200rpm
 * Take OD samples every 1-2hrs (grew slowely)
 * Add 0.5uM IPTG to culture with E.coli + pLac-fMT to induce fMT @ OD=0.6
 * Grow o/n