August/29 August 2009

1. Colony check of cultures transformed on 8/28. Results were as follows: Part label or Plate location          Approx. number of colonies (12)                                    10     (13)                                     20     (14)                                     20     (15)                                     20     (16)                                     over 100 (18)                                    3     (19)                                     20     (20)                                     0     (21)                                     0     1-6I                                     over 100

Plates (18), (20) and (21) were returned to incubator (37 degrees) to check for colony growth on the following day.

2. (12) to (16), (18), (19), and 1-6I were inoculated into growth culture solution. Miniprep should be conducted on the resulting cell cultures tomorrow.

3. (12), (15), (16) were streaked on a culture plate together with 1-23L as reference vector. Also, (13), (14), (19) were streaked together with 1-2M as reference vector. Ligation check (by electrophoresis) should be conducted on these tomorrow.

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