Duke University/18 June 2009

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June 18, 2009

 * E: Assembly PCR, Gels to check PCRs

2x Phusion mix 12.5 ul insert 7.14 ul H2O 5.36 ul 25 ul

98°C 30s 1x 98°C 10s 70-55°C 5s 72°C 30s 25x 72°C 2m 4°C infinity 1x

Fragment

Length of plasmid: 8360 bp + 4 Histags * 6 aa * 3 bp = 8432 bp
 * R: Smear because of misformed gel, no band
 * C: Change conditions