EPF-Lausanne/3 September 2009

  

3 September 2009

Wet Lab
There is clones on every plate!
 * Results of the RO1:
 * → colony PCR on all clones with the Taq Platinium protocol

All media have worked! (except for the SC) They have different colors depending on media and clone after an overnight culture.
 * Results from the media try:

We tried the following media, with and without Trp :
 * Culture for characterization
 * LB
 * M9/min + thiamine
 * M9/min + AA + glycerol
 * M9/min + thiamine

The gel showed :
 * RO1: it works fine, all our constructs have the TrpO
 * RO2: #6 and #7 do not have the construct (we took the wrong clones), while #8 seems to have the construct!

People in the lab
Basile, Mélanie, Caroline

 