File:Colony PCR of SmtA-1 - 05september2009.jpg

Colony PCR of SmtA colony #1 using Biobrick VF2/VR primers (lane 1) and RBSforward and SmtAreverse primers (lane 2). Probably a different RBS primer was used, explaining the lack of product. SmtA (including RBS) is 678 bp, PCR product using VF2/VR primers should yield a band of 989 bp. Left and right are both the same 1% agarose (1xTBE) gel on different time points.