Team:Chiba/Notebook/Calendar/17 September 2009



>Go to the Notebook page

(16_September_2009 <|>18_September_2009)

Mini Prep
10:30~
 * 1) I731012(1930bp) pSB1A2(2079bp)
 * 2) K091134(2048bp) pSB1AK3(3189bp)
 * 3) S03956(1041bp) pSB1A2(2079bp)
 * 4) K091118(1985bp) pSB1A2(2079bp)

Digestion Test
14:30~

Double Digestion

Single Digestion

Gel

agar(low electro-osmosis)---0.7%(W)---0.42 g

TAE---60 ml

Energization

135V 27min

Result

from left to right

marker 1 2 3 4(no treatment) marker 1 2 3 4(single digestion) marker 1 2 3 4(double digestion)

ALL:OK

Lux Mutants Sequence
18:30 Mini prep

21:00 PCR

Las Check
22:00 preculture LB-Amp 3 mL


 * 1) K084007
 * 2) K084012
 * 3) T9002
 * 4) K091134
 * 5) I731012

Lab Notebook