Team:Paris/7 August 2009

NoteBook
August 7th

 

Microscope
Microscopy We try using the time laps with two  filters

Molecular Biology
Reception of primers (oligos)
 * Primers from 08/03 arrived at Necker. References : O19 O20 O21 O22 O23 O24 O25 O26 O27 O28 O29 and O30.
 * All primers received by now are labeled and stored at -20°C in the "Oligos" box. Stock is 100µM. A 10µM solution (20µL) of each has also been prepared and is available in the "Oligos" box at -20°C. These 10µL solutions are to be used in priority.

Miniprep P15, P16, P17, P18, P19 Digestion P15, P16, P18, P19 by XbaI/PstI (Neb2), P17 by EcoRI/SpeI (Neb4) Migration Purification valeur Verification sur gel ON culture P1, P2, P17, S31 Overnight PCR compo  