Wisconsin-Madison/3 July 2009

July 3, 2009
Ex 18, 10: Cyanobacteria and pTaq

Taq and Cyano Genomic DNA

Ex 10: Cyanobacteria

inoculated new 7942 and 7002 liquid cultures in appropriate media Prep M9 minimum medium prep

Ex 19: Parts from iGEM Registry

Transform RFP and lac+GFP from iGEM plate - NC & XD Note: these are Kanamycin resistant

Get IPTG inducible GFP from Doug's lab as positive control

Ex 16: Cloning

Inoculate lac & NudF smear colonies(to prepare for gel extraction)

Canamycin resistance

Re transform and replated

RFP is innculated from this

Lac +GFP worked

But also test our GFP with lac

Look up lac+GFP and GFP is the same

95% Pro U is not working bec

Preped a bunch of Nud F colonies