Template:Team:KULeuven/29 August 2009/VanillinProduction


 * Split the restriction digest in 2 parts
 * 1 Part was directly used for ligation using double the mass required(SAMS II and EF II)
 * The 2nd part was first put on a gel electrophoresis and purified before it was used for ligation
 * Gel electrophoresis of restriction on sam5, sam8, fcs and ech
 * sam5 and sam8 showed expected results
 * fcs and ech had unexpected signals
 * fcs showed only 1 result on gel, expected two
 * ech showed 4 results in gel (all too short), expected one
 * sam5 and sam8 were purified and ligated (SAMS I)


 * ech and fcs were cut again and put overnight (over the weekend...) in 16°C