Team:Virginia/29 June 2009

Today

 * Discussion with Prof Kozminski
 * Ligation: Need to use agarose certified for in-gel ligation
 * Protein purification/detection: probably want to use HA tag since we just to show we're getting protein expression; affinity purification overkill; need to consider size of tag


 * Miniprep of untransformed DH5a just for control's sake
 * Cut ABP (X,P)
 * Ligate @15C A09-3(3): ABP (E,P) pSB1C3 (E,P)
 * Ligate @15C A09-5: ABP (X,P) & J13002(S,P)
 * Ligate @15C A09-6: ABP (X,P) & J04500(S,P)
 * Starch iodine As assay - order reagents
 * Modeling: Installing software