Team:TorontoMaRSDiscovery/10 June 2009

=June 10, 2009=
 * 1) Poured 10 Tet plates following procedure on June 5, 2009
 * 2) Due to problems with DNA ladder and restriction enzyme digest yesterday, procedures were followed again today with the following adjustments
 * 3) *DNA was diluted and run on lanes 1-5 of gel:
 * 4) **Lane 1 - 1X
 * 5) **Lane 2 - 1/6X
 * 6) **Lane 3 - 1/36X
 * 7) **Lane 4 - 1/10X
 * 8) **Lane 5 - 1/100X
 * 9) *Restriction enzyme digest was repeated with an incubation time of 4 h at 37 degrees Celsius and heat shock at 80 degrees Celsius was done in hot water bath
 * 10) *Results: Dilution did not work well (ladder could not be visualized). Digest appeared to work as 3 bands could be seen in last 2 lanes
 * 11) *Adjustments for tomorrow:
 * 12) **Spin down enzymes before using
 * 13) **Overnight digest