IBB Pune/30 September 2009

We performed many ligations today. They have been digested with the following enzymes and been ligated in the following sets:

Set 1:

Ak3-EP

GFP-XP

PR-ES

Set 2:

pLuxR-ES

LuxI- XP

Ak3-EP

Set 3:

PR-ES

GFP-EX

Set 4:

pLuxR-ES

LuxI-EX