EPF-Lausanne/8 July 2009

  

8 July 2009

Wet Lab
1. R. Palustris culture grew. A glycerol stock has been done. A pellet is on the fridge level 2, waiting for a miniprep.

2. iGEM parts have been transformed:

Cloning Strategy
Inducible LOVTAP biobrick strategy
 * Problem to overcome:
 * PstI sites in LOVTAP sequence.
 * Our goal:
 * Biobrick consisted of LacI promoter-RBS-LOVTAP-Term (in this order).
 * Material:
 * Biobrick of LacI promoter, RBS, LOVTAP, Term separately ( LOVTAP obtained from previous section and the rest from iGEM Spring 2009 distribution Kit plate).
 * Strategy:
 * LacI promoter-RBS ligation with iGEM protocol (LacI promoter digested with ES, RBS digested with XP, plasmid containing an other antibiotic digested with EP).
 * LOVTAP is digested with ES and inserted into Term plasmid (which was digested with EX previously).
 * Finally, LacI promoter-RBS digested with ES to be inserted into LOVTAP-Term plasmid, digested with EX.

People in the lab

 * Heidi, Tu, Nath, Rafael, Basile

 