ULB/23 July 2009



 iGEM Team:ULB-Brussels Wiki  

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Université Libre de Bruxelles

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 Home Team Project</a></li> Parts</a></li> Safety</a></li> Notebook</a></li> Sponsors</a></li> </ul> <ul>

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Results for the deletion program after running on a gel :


 * we get a band for the hfsG gene with DMSO. Parasite bands are very light.
 * hfsH gene: still lots of parasite bands…

Discussion on the PCR programs.

Maybe we should increase the annealing temperature or dilute the mix hfsH gene with DMSO ?

Gradient PCR on promoter : 44 to 56°C

Miniprep of bacterial culture to extract the assembly standart 21.