Team:Warsaw/Calendar-Main/24 August 2009

Making of the plac-RBS-llo-intA part Jarek

Tasks:  Preparation of 24 liquid cultures from colonies that grew after transformation in LB+Amp. Incubation of liquid cultures in 37C degree. Isolation of plasmid DNA from liquid cultures. Digestion of the isolated DNA with EcoRI endonuclease. 

Cloning switch 1 regulatory parts [ K177012-PcI.RBS.LacI, K177033-PcI.RBS.LacI.PcI.RBS.RFP.terminator, K177011-PLacI.RBS.cI.terminator, <a href="http://partsregistry.org/Part:BBa_K177038">K177038</a>-PLacI.RBS.cI.terminator.PLacI.RBS.GFP.terminator ] into two compatible low copy number plasmids of different antibiotic resistance Ania Tasks:   </li> </ul>