Team:Kyoto/GSDD/Notebook/0817-0823



 /* ul#navi_GSDD or ul#navi_CiC */ /* li.nav_i, li.nav_me, li.nav_g, li.nav_r, li.nav_p, li.nav_mo, li.nav_n */

background-position:left bottom !important; color:black; }
 * 1) navigation ul#navi_GSDD li ul li.nav_n a{
 * 1) Home
 * 2) GSDD
 * 3) Notebook

To Do

 * PCR
 * Restriction Enzyme Digestion
 * Electrophoresis
 * Gel Extraction kit
 * 3-O-2
 * pSB1A2

3-O-2

 * primerF:AACTGCAGAACCAATGCATTGGagaacgtctctcactgaac
 * primerR:aaaattttGGACTAGTCCttgaatcttagcccccttg


 * Length
 * 3-O-2+BBa_J63010:6103bp
 * 3-O-2 PCR product:2851bp

We can not find the bands of PCR products. So, we will do PCR again, changing the volume of MgSO4, 3ul, 4.5ul, 6ul.

pSB1A2



 * Length
 * pSB1A2:2079bp

We use pSB1A2 in order to insert PCR products and sequence them. But the concentrations of this sample are very low. So, we will try again.

To Do

 * PCR
 * Restriction Enzyme Digestion
 * Electrophoresis
 * Gel Extraction kit
 * 3-O-2
 * pSB1A2

3-O-2

 * primerF:AACTGCAGAACCAATGCATTGGagaacgtctctcactgaac
 * primerR:aaaattttGGACTAGTCCttgaatcttagcccccttg


 * Length
 * 3-O-2+BBa_J63010:6103bp
 * 3-O-2 PCR product:2851bp

pSB1A2

 * Length
 * pSB1A2:2079bp

We use pSB1A2 in order to insert PCR products and sequence them.