Minnesota-experimental/1 July 2009

- Flow cytometry data was collected for samples from 6-29. (TTL, mutant at position 3).

- PCR products from 6-30 were cleaned using a QIAquick column protocol.

- TNN, TTN, and TTL promoter constructs mutant at position 5 were ligated into the pGlow-TOPO vector (Invitrogen).

- Ligation products were transformed into TOP10 cells; cells were spread on antibiotic LB agar plates and allowed to grow at 37 °C overnight.