Team:Wash U/Calendar-Home/7 July 2009

Tuesday July 7, 2009
- Ran Gel of digestion and ligation products from Biobrick Assembly

- Performed colony PCR on colonies transformed with plasmid from ligation reaction Results: Gel Colonies Red colonies indicate incorrect ligation

- Made glycerol stock solutions: 1. pSB1AC3 2. puc19 E. coli 3. BBa_I15010 pSB2K3 4. S17-1 E. coli 5. BBa_R0082 pSB1A2 6. pSB1T3 7. BBa_E0240 pSB1A2 8. BBa_B0034 pSB1A2 9. pSB1AT3 10. pRKCBC3 11. pSB1A3 12. BBa_B0015 pSB1AK3 13. pucHS27 14. pSB1K3 15. R. sphaeroides 2.4.1

-Purified genomic DNA of E. coli and R. sphaeroides for PCR DNA Quantities: 1. E.coli (pellet from 1.5 mL of culture): 54.5 ng/uL 2. E.coli (pellet from 3.0 mL of culture): 70.0 ng/uL 3. R.sphaeroides (from 3.0 mL of culture): 62.5 ng/uL 4. R.sphaeroides (from 1.5 mL of culture): 40.5 ng/uL

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