Team:Southampton

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">   University of Southampton Wiki    /*FOOTERPUSH*/ width:1px; height:1202px; } /*FOOTER*/ position:absolute; left:102px; top:1247px; z-index:100; } position:relative; left:31px; top:-25px; width:50px; height:20px; z-index:100; } position:absolute; left:795px; top:275px; width:164px; height:333px }	position:absolute; left:806px; top:587px; width:137px; height:107px } /*TOOLBAR*/ position:absolute; left:-2px; top:0px; width:978px; height:27px; z-index:100; }	position:absolute; left:29px; top:34px; width:329px; height:108px; z-index:0; }    Home  Project <![if gt IE 6]><![endif]>  <li class=" cssMenui">Project Aims</a></li> <li class=" cssMenui">Project Introduction</a></li> <li class=" cssMenui">Project Description</a></li> <li class=" cssMenui">Project Achievements</a></li> <li class=" cssMenui">Project Projection</a></li> <li class=" cssMenui">Project Safety</a></li> <li class=" cssMenui">References and Links</a></li> <li class=" cssMenui"> Protocols <![if gt IE 6]></a><![endif]> <ul class=" cssMenum"> <li class=" cssMenui">Protocol Overview</a></li> <li class=" cssMenui">Protocol Theory</a></li> <li class=" cssMenui">Biobrick Transformation</a></li> <li class=" cssMenui">Colony PCR</a></li> <li class=" cssMenui">DNA Double Digestion</a></li> <li class=" cssMenui">Gel Electrophoresis</a></li> <li class=" cssMenui">High Fidelity PCR</a></li> <li class=" cssMenui">LB Plates</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Project/Protocols/Ligation">Ligation</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Project/Protocols/Ligationtransformation">Ligation Transformation</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Project/Protocols/PlasmidPrep">Plasmid Preparation</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Project/Protocols/TA">TA Cloning</a></li> </ul> </li> </ul> </li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Parts/GoL">Biobrick Parts<![if gt IE 6]></a><![endif]> <ul class=" cssMenum"> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Parts/GoL">Game of Life</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Parts/RPS">Rock Paper Scissors</a></li> </ul></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Team"> Team <![if gt IE 6]></a><![endif]> <ul class=" cssMenum"> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Team">Team Profiles</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Team/Advisors">Advisor Profiles</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Team/Southampton">Southampton Profile</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Team/University">University Profile</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Team/Gallery">Gallery</a></li> </ul> </li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Modeling"> Modelling <![if gt IE 6]></a><![endif]> <ul class=" cssMenum"> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Modeling">Modelling Overview</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Modeling/GoL">Game Of Life</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Modeling/RPS">Rock Paper Scissors</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Modeling/Open">Open Access</a></li> </ul> </li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/HP"> Human Practices <![if gt IE 6]></a><![endif]> <ul class=" cssMenum"> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/HP">Overview</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/HP/Outreach">Outreach</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/HP/igemexper">iGEM Experience</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/HP/Political">Political Awareness</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/HP/Legacy">Our Legacy</a></li> </ul> </li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Notebook">Notebook</a><![endif]> <ul class=" cssMenum">
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<li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Notebook">Notebook</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Notebook/Labbook">Lab Book</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Notebook/Advisorblog">Advisor Blog</a></li> </ul> </li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Sponsors"> Acknowledgments <![if gt IE 6]></a><![endif]> <ul class=" cssMenum"> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Sponsors">Sponsors</a></li> <li class=" cssMenui"><a class=" cssMenui" href="http://2009.igem.org/Team:Southampton/Southampton">Team Southampton</a></li> </ul> </li> </ul> <img style="width: 975px; height: 1005px;" id="bg" src="http://www.personal.soton.ac.uk/jeh2v07/images/BGHOME.gif" alt="" /> <img style="width: 63px; height: 63px;" alt="" src="http://www.personal.soton.ac.uk/jeh2v07/images/logobox.jpg" /> Project Description

01/08/2009 - Project As a new team to the iGEM competition, Southampton hopes to bring an innovative application of quorum sensing to the 2009 Jamboree. Using this method of bacterial communication, we have designed two systems, which demonstrate the classic games &lsquo;Rock-Paper-Scissors&rsquo; and &lsquo;Conway&rsquo;s Game of Life&rsquo;.We aim for these complementary systems to exhibit complex intercellular behaviours arising from the application of simple &lsquo;game&rsquo; rules. John Conway&rsquo;s Game of Life is a popular representation of cellular automata. As a zero player game, it proceeds without further input once initiated. We intend to utilize the Game&rsquo;s basic rules to develop a system on two separate plasmids that fluoresces in an imitation of the Game. Thereby we aim to initiate and thus control the creation of patterns produced by the oscillating bacteria.

Rock-Paper-Scissors&rsquo; is a well-known two-person hand game whereby the players seek to produce a gesture that will defeat that of their opponent. By coordinating the promoters and operators of three different bacterial cells, we have designed a system where each bacterium can disable the primary plasmid of specific opponent cells, whilst simultaneously enduring the attacks of their rival cells. Our aim is to have no bias within the system so that the end result is not predetermined. Learn more about the iGEM competition <a href="http://2009.igem.org/About">here</a> and about synthetic biology <a href="http://en.wikipedia.org/wiki/Synthetic_biology">here.</a> Updates 21/09/2009

Team <img style="width: 296px; height: 164px;" alt="" src="http://www.personal.soton.ac.uk/jeh2v07/images/team.jpg" /> <a href="http://www.wessexscene.co.uk/features/3394"><img src="http://2009.igem.org/wiki/images/0/04/Scene.png" alt="" width="329" height="108" border="0"/></a> <a href="http://www.facebook.com/home.php?#/group.php?gid=107692312882"><img style="width: 97px; height: 100px;" alt="" src="http://www.personal.soton.ac.uk/jeh2v07/images/facebook.jpg" /></a> From left :- Steph, Danie, Tom, Jon, Jo, Paul, Karolina, Claire, Richmond. For more information on our project, <a href="http://2009.igem.org/Team:Southampton/Project">Click Here.</a>

To find out more about us, go to the Team Page or look us up on <a href="http://www.facebook.com/home.php?#/group.php?gid=107692312882">facebook</a>. Final week, and what do we have to show for our iGEM experience? We performed TA cloning on our novel biobricks; pTac and GFP. It is the first (and only) successful ligation so far… We have a final meeting and farewell with a few refreshments… For more click <a href="http://2009.igem.org/Team:Southampton/Notebook" style="font-family: Arial, Helvetica, sans-serif; font-size: 13px;">here.</a> <a href="http://2009.igem.org/Team:Southampton/HP/igemexper"><img src="http://2009.igem.org/wiki/images/9/98/Experience.jpg" width="134" height="105" border="0" /></a>

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Sponsors

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