Lab July 14 2009

Came in to check on DH5α plates. No obervable growth.

I put two of the four plates in the 37 degree incubator to see if anything would grow.

I took the remaining SOB plate and asked crystal to show me her streak technique. She uses a sterile wooden stick to spread a good large chunk of frozen cells onto the first quadrant of the plate, then a second sterile stick to streak across that first quadrant onto the rest of the plate.

This is hopefully where the problem lies.

Allison and Crystal confirmed that we do not need to regenerate seed stocks, so tomorrow if there is colony growth we can innoculate the 250 ml liquid medium directly from the plate. Since we are growing the plate at room temperature (per protocol, and Hanahan paper, but contrary to Crystal's usual procedure) we need to give it at least 24 full hours before we can expect to see growth.

So tomorrow will be a short one. Late Thursday afternoon or first thing Friday morning before we are likely to get to the cell density we need for centrifuging...

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