Team:Brown/Notebook Meetings/5-4-09

iGEM 2009 Meeting

May 4, 2009


 * 6:13PM: Meeting starts in SFH Auditorium. Discussed War War Ko’s name.
 * 6:14: Project ideas. Adding Minoo’s “Pro Bread” to the list
 * Yeast that makes amino acids (I, L, V) and packages
 * 6:25: Pulling out E. Colirs
 * 6:29: Stephanie suggests T shirts
 * 6:31: http://2009.igem.org/Team:Brown
 * This is the iGEM Team Wiki that we can customize
 * 6:37: Tick and Treat
 * Need to find out what histamine does after it binds to a histamine receptor
 * EV131 HBP is a potential part to submit
 * Addressed the liver targeting protein issue – need to control this to make sure it grabs a histamine before it is uptaked by liver
 * Delivery system
 * Timeline?
 * 7:01: Safe Cell
 * Will – “This is how we can prevent government regulation”
 * Implementation: RFC biobrick – as part of the standard for making a device, you would have to have these genes included.
 * Applicable to prokaryotes and eukayotes
 * Media vs gel – we can put the repressor in the gel
 * Refine holin slide
 * Transcriptional repressor implementation?
 * Indu will make it asthetically pleasing


 * 7:21: Pro Bread
 * Background is good but we should get to the point quicker
 * Issues: do yeast have all the enzymes to make the essential amino acids?
 * Yes. If so, then we can upregulate these enzymes
 * Can we produce gram quantities?
 * Will depleting pyruvate affect cells ability to make amino acids because we are depleting some of its ATP source
 * Can we FIND the rate limiting step of yeast ILV biosynthesis pathways? Upregulating this step will produce more amino acids
 * Negative feedback – can we prevent this? Packaging?


 * 7:54: Eli’s Cog Sci Project
 * 7:58: Histamine: Indu, Eli, Ashley
 * Safe Cell: Ahmad, Michael, Flora
 * Pro Bread: Minoo, Will, Steph


 * 7:59: NEXT MEETING: Thursday afternoon 3:00 PM
 * BRING YOUR FINAL POWERPOINTS!! And make the changes.