Minnesota-experimental/6 July 2009

- TNN, TTN, and TTL promoter constructs mutant at position 6 were constructed by SOEing PCR.

- SOEing PCR products were cleaned using a QIAquick column protocol. Cleaned products were amplified by PCR and stored at 4°C.

- Plasmids containing TNN, TTN promoter constructs mutant at position 4 were transformed into DH5αPro cells. Transformed cells were spread on antibiotic LB agar plates and grown overnight at 37 °C.