Team:UAB-Barcelona/Primersn

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These were the primers we ordered for the nitrite:

•	Pnir: 		EcoR1-Not-Xba— nirk – Spe

Reverse: S    GTCAACGT ACTAGT CTGCACGAAAGACCTTTAAATAC    Annealing temperature = 62 ºC

Forward: E     N       X      CAGTAGTA GAATTC GCGGCCGC TCTAGA GTTGCGCGAAATACCGTAAC   Annealing temperature= 60 ºC

•	nsrR:		X – nsrR – S – N- P

Reverse: X    RBS    Linker GTCAACGT TCTAGA TAAGGAGG AATATAA ATGAGACTGACGAATTACAGCGATTAC  Annealing temperature = 76 ºC

Forward:

P     N       S      CAGTAGTA CTGCAG GCGGCCGC ACTAGT GCCAAGCTGACCTACAGTTTATTCC       Annealing temperature = 76 ºC