IBB Pune/18 July 2009

Sharvari transformed DH5aplha cells with BBa_J04450 using 4 ul DNA. The plates were spread by Samit. Meanwhile Swetha inoculated pGFP for the next day's plasmid isolation.

We also discussed if the model that Samit had designed was applicable to our expected working conditions. Conclusion: find out kinetics of all the reactions involved.

plasmids were not separating on TBE. Praveen sir told us to use TAE instead of TBE for plasmid gels(0.8%) and to run the gels at 10 volts overnight.

Made TAE. Ran isolated pGFP samples.