Uppsala-Sweden/7 August 2009

Overview sheet
[[Media:Transformation Workorder.pdf]]

Ligation of ADH2(Z),pirB, pdc, PpetE, adh2(Y) into pSB1A3
Ligation with the pSB1A3 vector using the Quick Ligation Kit from NEB. Calculated amounts can be found here [[Media:Ligation Calc 07082009.xls]]

Transformation of ADH2(Z),pirB, pdc, PpetE, adh2(Y) into competent E. coli cells
Transformation was performed as written in the protocol form the Ligation kit from NEB

--Karl.brune 08:56, 10 August 2009 (UTC)