Virginia Commonwealth/27 May 2009

Tasks

 * 1) Transform NEB10beta with J04450 and I13521, using frozen stock of comp. cells.

Results

 * 1) Kept all tubes and cuvettes on ice at all times, and thawed one tube of NEB10beta competent cells (250uL).  Each of the following transformation was performed individually:
 * 2) * (-) control, with no DNA added
 * 3) * 1uL J04450 and 50uL cells
 * 4) * 2uL J04450 and 50uL cells
 * 5) * 1uL I13521 and 50uL cells
 * 6) Added 50uL cells to cold microcentrifuge tube.
 * 7) To cells, added appropriate volume and type of DNA, mixing well by pipette, and returning to ice to incubate for 1min.
 * 8) Transfer mix to cold 2mm electrotransformation cuvette, tapping to get mixture to the bottom.
 * 9) Pulsed cuvette on Ec2 setting, then immediately added 1mL LB.
 * 10) Transferred mixture to 15ml centrifuge tube and incubated for 30min at 37*C.
 * 11) Spread 35uL on either kan (J04450 and ctrl) or amp (I13521), incubated at 37*C overnight.