Template:Team:KULeuven/6 September 2009/VanillinProduction

A miniprep and restriction digest (ON) of sam5, sam8, ech and fcs was performed on Saturday. On Sunday, a gel was run to check the inserts. These were good so a motherplate for all the genes could be made.
 * Restriction digest overnight of sam5 (1,2) and sam8 (1,2)
 * Growth of EF Ligation (woohoo!)
 * Transferred 4 colonies from EF ligation to a new plate
 * Put the 4 transferred colonies on liquid medium