Team:TorontoMaRSDiscovery/21 May 2009

=May 21, 2009=
 * 1) Retrieved autoclaved ddH20, glycerol solution
 * 2) Gel Electrophoresis (test run)
 * 3) *1% gel: 0.5 g agar mixed with 50 ml of 1X TBE
 * 4) *10X TBE diluted with 5 ml TBE + 45 ml ddH20 – did not use all 50 ml for 1 gel (gel will be too thick – make 2 instead)
 * 5) *Loading Dye: add glycerol solution to 25 mg bromophenol blue then add dH20 to 10 ml (to make 6X)
 * 6) *Running gel: match wells to black side, run at 120 mA
 * 7) Visualize Gel in UV
 * 8) Turn power on
 * 9) Gel in machine face up
 * 10) Close door securely
 * 11) Turn white light on
 * 12) Adjust zoom, contrast, focus from black dial on top of machine
 * 13) Turn white light off (turns on UV)
 * 14) Press ‘live’ toggle – acq. Should be 0.4 sec.
 * 15) Print if desired or save on floppy disk
 * 16) Turn power off
 * 17) Dispose of gel in proper container
 * 18) Close door