Team:LCG-UNAM-Mexico/Resources/Protocols/ColonyPCR

Colony PCR Take the colony and resuspend in 200uL of TE 10:1 NaCl 10M Heat the suspension for 10 minutes at 95°C Centrifugue for 2 minutes at 14000 RPM Use 10uL of this DNA extract as template for the PCR

The mixture to perform the PCR is: 23uL H2O 2.5uL Forward primer 2.5uL Reverse primer 3.5uL dNTPs 2.5uL MgCl2 5uL Buffer10X 10uL DNA template 1uL taqDNA polymerase.