Illinois/9 July 2009

July 9
3'-Acting sRNAs

We cloned the GadY gene as well as the 3' UTR of gadX. We used two different primers for gadX because we weren't sure which design would work the best. Primer 2 goes all the way to the start codon of gadW.

We did this with a successful PCR.



We then purified the PCR product that was not used in the gel. We also prepared an overnight of dh5&alpha; transformed with pXG-1.

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