Team:TorontoMaRSDiscovery/11 June 2009

=June 11, 2009=
 * We did not have enough isolated plasmid DNA to justify doing another gel so we decided to perform another gel so we decided to perform another miniprep to get some more
 * Used DB3.1 transformed bacteria from shaker(measured spec 0.794)
 * Miniprep
 * potential issue our microcentrifuge only spins @ 10,000 not required 12,000
 * Binding DNA
 * did both optional steps preheated TE + washed w/ W10


 * measured UV absorbance = 4.2ng/ul
 * in all there is ~ 74 ul of TE should indicated ~370 ng of plasmid.
 * Digest
 * NOTE: plasmid length w/e biobric is 3189 bp biobrick length is 3255bp this can explain lack of 2 clear bands on gel after ligation.
 * nevertheless, we will perform another Gel to make sure we can properly run ladder to determine fragment sizes


 * digest measurements: 250ng plasmid all else half except BSA