Illinois/18 June 2009

June 18
We attempted another PCR of plasmid pJU-334 and ran a gel on it, using all 45 &mu;L of the reaction. We detected a faint band at ~3.1kbp (the expected size of the plasmid) and a strong band at ~1.9kbp. We purified the ~3.1kbp band and ran another PCR on the purified DNA.



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