August/22 August 2009

1.transformed check Checked the cell cultures transformed and plated out yesterday (8/21) for colony formation and made an approximate count of the number of colonies.

(ligation sample No.) (no. of colonies) ⑤                 failure ⑦                   100~        ⑧                    100~


 * ⑤  try back (digestion and ligation)
 * ⑦,⑧    ligation rapid check

2.Min prep Result of Nanodrop concentration check (sample No.) (concentration) 1-13N        30.3 ng/uL 2-8G         15.5 2-6O         24.4 26.0    ③-C          15.8 ④-A         25.4 ⑥-A         16.2

3.transfromation ligation sample ⑨, ⑩ , ⑪

4.digestion and ligation

K204005

K204012

K204013

K204014

K204015

↓ 37°C, overnight

back to NOTES