Team:Paris/21 July 2009

NoteBook
July 21th  

Brain work
Waiting for the return of Mr. Labs and his signature for the commisions.

Meeting at 14:


 * redistribution of the role and global progress :
 * g3p: raiders of the lost phage ... We will maybe amplify the g3p by ourself with a phage m13.
 * snare: there is still hope with the future plasmide to come.
 * fecA: demanding construction but still in slow progression
 * TMG: new idea to bypass our transduction problem .... let's say that the research begin.

 

Lab work
Overnight at 37°C, 200rpm
 * Medium for [S1] DH5alpha and [S2] K12 MG 1655 delta recA::Kan
 * 5ml of LB + Clone of [S1]
 * 5ml of LB + 5µl of Kan 1000x + Clone of [S2]

 

To do list

 * Glycerol stock for [S1] and [S2]