Team:Tokyo-Nokogen/Project

   







 The first induction with green light induces the expression of antigen 43, which causes the E.coli to self-aggregate and settle to the bottom. The medium is then decanted and replaced with a small volume of buffer. The aggregated cells are once again irradiated with green light, inducing the expression of holin and endolysin, which causes the autolysis of the bacterial cells. ESCAPES will allow us to escape from the annoying centrifugation and lysis steps, to conveniently produce crude extracts of our target protein.

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