IBB Pune/3 August 2009

experimenting with plasmid isolation protocols


 * Add glucose to solution 1 in Birnboin and Dolly.
 * ReSuspend plasmid in DI water instead of TE
 * Increase Lysozyme from 25mg/ml to 100mg/ml
 * Decrease incubation time with solution 2
 * Make fresh Phenol-Chloroform.
 * Go back to Chen and Liu, adn Kado and Olsen protocols.

phew, by the end of it we are left wiping sweat from our brows....