Team:Warsaw/Calendar-Main/5 August 2009

Cloning of p53 coding sequence Marcin Task 1:  Prepare the bacterial cultures for isolation of p53 CDS inserted into  pSB1A3  Preparation of bacterial cultures:  Prepare LB medium with kanamycin Add 3.5 ml of the medium to the probes Add one bacterial colony to each probe Breed the bacteria about 7 hours Assembly of endosomal detection operon Marcin  Transformation of chemocompetent E. coli strain DH5&alpha;</li> </ul> Methods:  Detailed protocol of transformation is described here</a>. The only modification is usage of total volume of ligation mixture prepared 04.08.09</a></li> petri dish will be held in 37&deg;C for 16 hours</li> </ul>