Team:Groningen/Notebook/30 August 2009

=Weekend stuff=

Metal accumulation
There was doubt on the identity of the vector in which the inducible promotors were build, seeing as it did not grow on LB supplemented with chloramphenicol it is most probably not pSB1AC3, perhaps pSB1A2. Cultures were put in glycerol stocks, except for pBAD & pLac whose identity first need to be confirmed.

Transformation
was in fact pSB3K3! Therefore no colonies could grow. 5 colonies of each promotor were inoculated for an ON culture, colony PCR was postponed to monday.

Transporters
HmtA

the second PCR1.2 and PCR2 worked