Team:Gaston Day School/7 July 2009

 Spin 1.5mL of overnight culture for 30 sec (5') in microfuge Aspriate off all but 100x of the supernatant and resuspend the pellet by vortexing Add 30x of TENS and mix by inversion. The solution should become viscous. Add 150x of sodium acetate and vortex. A fine, white precipitate should form Centrifuge for 2.5 min at 10k TRANSFER the supernatant to a clean tube and add 2 volumes (1mL) of room temperature EtOH Vortex and pellet DNA by centrifugation for 2-5 minutes at 10k Wash pellet with 70% ethanol and allow the pellet to dry</dt> Resuspend the pellet in 30x of TE with RNAseA</dt> Digest:</dt> 10x DNA</dt> 3x Buffer</dt> 15x H2O</dt> 2x Xba</dt> 2x pst</dt> Threw out RFP</dt> Made more TENS</dt>