Team:UC Davis/Project1/Finding ph sensor

             <span style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"> </b><b style="color: rgb(255, 255, 153);"><span style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">   <img alt="" src="http://2009.igem.org/wiki/images/b/b9/UCDAVIS_PIC8.png" style="border: 0px solid ; width: 78px; height: 36px;"></a>   </b><b style="color: rgb(255, 255, 153);"><span style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">   <img alt="" src="http://2009.igem.org/wiki/images/2/2f/UCDAVIS_PIC5.png" style="border: 0px solid ; width: 81px; height: 36px;"></a>   </b><b style="color: rgb(255, 255, 153);"><span style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"> </b><b style="color: rgb(255, 255, 153);"><span style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;">   <img alt="" src="http://2009.igem.org/wiki/images/a/a6/UCDAVIS_PIC6.png" style="border: 0px solid ; width: 78px; height: 37px;"></a> <img alt="" src="http://2009.igem.org/wiki/images/1/1d/UCDAVIS_PIC7.png" style="border: 0px solid ; width: 83px; height: 37px;"></a>   </b><b style="color: rgb(0, 0, 0);"><span style="font-size: 13.5pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"> </b> <hr style="width: 100%; height: 2px;"> <p class="MsoNormal" style="background: white none repeat scroll 0% 50%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left; text-decoration: underline;"> <span style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;">Finding an appropriate biological pH sensor: </b> <p class="MsoNormal" style="background: white none repeat scroll 0% 50%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left;"><span style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;">Why is there a need for a pH sensor?<o:p></o:p> </b> <p class="MsoNormal" style="background: white none repeat scroll 0% 50%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left;"><span style="font-size: 12pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;">          We would like our probiotic organism to confine itself to the stomach. Since low pH is a hallmark of the stomach, it seems reasonable to assume that linking the production of a suicide gene to a dramatic increase in pH (to values found in the intestine) should allow us to sequester our organism to the stomach. There are no pH sensors available on the registry of biological parts. So, we will engineer a device to sense extracellular pH changes and design it so that it can be used to regulate the expression of any target gene in confinement by pH levels, and then add it to the registry!<o:p></o:p> <p class="MsoNormal" style="background: white none repeat scroll 0% 50%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left;"><span style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;">Finding an appropriate biological pH sensor: </b><span style="font-size: 13pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><o:p></o:p> </b> <p class="MsoNormal" style="background: white none repeat scroll 0% 50%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left;"><span style="font-size: 12pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;"> We have decided to design our pH sensor by adopting one of the pH sensors of <i>Agrobacterium tumefaciens</i>. The ChvG/ChvI two-component system of A.tumefaciens is suspected to sense extracellular pH changes and relay this environmental signal to numerous virulence-related promoters(2). <o:p></o:p> <p class="MsoNormal" style="background: white none repeat scroll 0% 50%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left;"><span style="font-size: 12pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;"> For more information regarding  <span style="font-style: italic;">ChvG/ChvI two component system <span style="font-size: 12pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;"><i>, go to ChvG</a>, ChvI</a>, ChvG---&gt;ChvI</a> </i> <hr style="width: 100%; height: 2px;"> <p class="MsoNormal" style="background: white none repeat scroll 0% 50%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left;"> <span style="font-size: 12pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;"> <span style="font-size: 12pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;;"><i><span style="color: blue;"> </i></a><o:p></o:p> <p class="MsoNormal" style="background: white none repeat scroll 0% 50%; line-height: normal; -moz-background-clip: -moz-initial; -moz-background-origin: -moz-initial; -moz-background-inline-policy: -moz-initial; text-align: left;"><i> <span style="font-size: 12pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: blue;"> </i><span style="font-size: 12pt; font-family: &quot;Times New Roman&quot;,&quot;serif&quot;; color: black;"><o:p></o:p>