Team:Warsaw/Calendar-Main/9 September 2009

Acquiring the internaline A gene from Listeria monocytogenes st. EDG-e genome
Jarek

Tasks:
 * Transformation of competent E.coli strain TopF' with ligation.
 * Plating ligation on LB+X-Gal+IPTG+Amp plates and leaving for incubation in 37C degree.

Assembly of fusion proteins Marcin Task 1 (done by Kuba):
 * Prepare the bacterial cultures for isolation of following construct:
 * mitochondrial signal peptide from Cox CDS on pKSII plasmid

Task 2 Methods:
 * isolation of previously described construct
 * Plasmids were isolated using the A&A plasmid mini kit. Detailed procedure of the isolation is described here

Inverting PLacI regulated parts of the switch and assembling the switch on a single plasmid Ania Tasks:    