August/8 October 2009

1.sequence check Today we conducted PCR in order to sequence the parts we have built over the summer. Using the chain-termination method and standard sequencing primers from the Parts Registry, we obtained chain-terminated clones of each sample and put them through an automatic DNA sequencer. The results will be picked up and analyzed tomorrow.

2.colony check sample       no. of colony 2-6O               +++ 11                  +++    20                   ++   21                   +++  1-15L               +++ 1-15J             +++ 52                   20    67                     2    68                     0    69                     0

3.ligation sample 70, 71 , 74 , 75

4.digation

K204059

K204072

↓ 37 degree, 3hr gel cut & ligation

5.transformation sample 70, 71 , 74 , 75 , X4 , X4

6.color intensity check plac+color, ptet+color

7.sequence check sample 22, 47 , 40 , 25 , 51 , 50 , 31 , 32

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