Team:TzuChiU Formosa/Result

  

Result
1.

'''Figure.3(a.)pSB1A3 digestion gel image. Start with left to right:(1.)100bs marker, (2.)Uncut pSB1A3, (2.)Uncut pSB1A3, (3.)Cut pSB1A3; (b.) (1.)100bps marker, (2.)Dephosphorelation of Pst1 cutting site in pSB1A3.

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When we confirm the PSB1A3 that contain OmpC promoter. And we amplify it, used Pst1 to cutting off the sequences and run the gel electrophoresis. Due to the cutting form plasmid will change circular to linear form caused molecular weight increasing so the gel electrophoresis speed will slow down and stop at the upper strata show at the Fig.3-a.

In order to improve the successful rate of putting insert into the vector we treated, Pst1 digested pSB1A3 with calf Intestinal Alkaline Phosphatase (CIP) to remove the phosphate group thus reducing the chance of self ligation