Team:KU Seoul/Overview

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Overview

 * Integrated Heavy Metal Detection System
 * Our team project is designing synthetic modules for simultaneous detection of multiple heavy metals such as
 * arsenic, zinc, and cadmium in E. coli. The ultimate goal is to build a micromachine sensing and determining of
 * the concentration of heavy metals in a sample solution (e.g. the waste water). In order to design the system,
 * we will employ two fluorescence proteins (GFP and RFP) and aryl acylamidase as signal reporters. Since each
 * heavy metal promoter produces unique fluorescence or color by those reporters, if more than two heavy metals
 * coexist in a solution, the results would be interpreted from the convoluted fluorescence and/or color rather
 * than a single signal detection. The successful construction of the synthetic modules in E. coli can be utilized
 * in the form of a lyophilized powder, which can be stored in a drug capsule to make it portable.


 * STEP1, Prepare plasmids.


 * STEP2. Gene cloning & Infusion Biobrick assembly(SLIC)
 * Using the SLIC method, infuse the biobricks inside of plasmid. There are two inserts just made through SLIC method.
 * [[Image:insert_yodA_amd.png|center]]
 * STEP2. Gene cloning & Infusion Biobrick assembly(SLIC)
 * Using the SLIC method, infuse the biobricks inside of plasmid. There are two inserts just made through SLIC method.
 * [[Image:insert_yodA_amd.png|center]]
 * Using the SLIC method, infuse the biobricks inside of plasmid. There are two inserts just made through SLIC method.
 * [[Image:insert_yodA_amd.png|center]]


 * STEP3.measuring the concentration of heavy metal
 * STEP3.measuring the concentration of heavy metal


 * [[Image:cd2+_detector.png|center]]
 * This picture is showing cadimum detection. Another case is similar to this experiments.


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