Imperial College London/Notebook/6 October 2009

Dineka

 * Midi on RcsB pst1
 * Midi on PAH
 * Mini's (x12) on OP 1-6 and OP+His 1-6
 * Restriction Digest on Dam New 1-8 as sequencing failed 1st time round - wrong DNA sent?!
 * Midi culture set off overnight for CRP-RBS+AK3 colony 1 from replica plate 17/9/09.
 * Housekeeping - tips (all sizes), 1.5ml epis, 2ml epis, and pcr tubes sent to autoclave.

Protocol considerations

 * Cells were grown overnight in 5ml of M9 media
 * Cells were then diluted and grown at 28 and 42 degrees
 * The cells were allowed to acclimatise to their respective temperatures for 3 hours before the temperature shift took place

Results


[[Media:Hvd-GFP 06-10.xls| Download raw data]]

Conclusions
Waiting for data analysis

Suggestions/Improvements
Waiting to be uploaded