Team:Newcastle/IntroductoryLabwork/6 July 2009

Introductory Lab Session: 6th July 2009
List of bricks for the day
 * 1) BBa_J04450 (plasmid:pSB1AK3). Location: Plate 1, Well 13A
 * 2) BBa_I13522 (plasmid:pSB1AK3). Location: Plate 2, Well 8A
 * 3) BBa_J04450 (plasmid:pSB1AT3). Location: Plate 1, Well 15A

Preparation of the tubes

 * We got 3 tubes containing cells from the freezer at 80C and placed them into the ice and waited for 30 minutes
 * We prepared 6 tubes (3 tubes for controls and 3 tubes for the plasmids) for the bricks
 * We added 40ul of cells to each of the six tubes
 * We added 3ul of DNA for the plasmid tubes and 3ul of water for the control tubes
 * We added 900ul of LB for each of the six tubes
 * We then placed the tubes in the shaking incubator for an hour.

Plating out
We prepared 9 plates with full strength (undiluted) and 9 plates with diluted tube content.


 * Plates with undiluted content
 * The first three plates having LB + Amp were used as the negative control. Hence the cells not having amp. resistance would not grow on these plates.
 * The next three plates with LB content were used as the positive control. Cells without amp. resistanec would still grow on these plates.
 * The other three plates were used for the plasmids.


 * Plates with diluted content
 * For the diluted ones we used 900ul of LB and 100ul of DNA from the corresponding tubes.
 * The same set of 9 plates were prepared for the diluted content.