Team:Warsaw/Calendar-Main/28 May 2009

Cloning of hly gene into pKSII+ vector Marcin  Task:  Restriction digest of isolated plasmids pKS/hly to confirm the correctness of the ligation Methods:  Composition of reaction mixture: 1 &mu;l purified plasmid solution 0.5 &mu;l HindIII (Fermentas) 2 &mu;l Buffer Red (Fermentas) 15.5 &mu;l MQ water  Digest  Program: description of the program is here After digest samples were loaded into gel and electrophoretically separated Electrophoresis conditions: voltage - 100V time: 40 minutes  Fig.1. Restriction pattern of analysed samples Comment:

Sample 1 and 2 have expected resctricion pattern.