Virginia Commonwealth/6 July 2009

Results

 * n/a

Tasks
Maria and Afton Trentay 19:36, 6 July 2009 (UTC)
 * Gather information and choose parts for experiments (promoter, backbone, and RET)
 * Write running draft
 * Transfer information to new lab notebook

Kevin and Adam
 * Continue research and further analyze and hone the list of DNA sequences to be synthesized

Craig and Clay
 * It was decided to continue work done by the 2008 Eidenburg team by engineering a metabolic pathway for limonene synthesis. Three parts are currently available in the distribution plates (BBa_I742111, BBa_K118024, and BBa_K118025).  BBa_I742111 contains a ribosomal binding site attached to the limonene synthase gene (LIMS1) from citrus lemon.  BBa_K118024 contains the former as well as ribosomal binding sites and a dxs gene and appY gene.  BBa_K118025 contains the ribosomal binding sites and the three aforementioned genes as well as a promoter.  We will begin by electrotransforming these parts into the NEB 10 beta strain of E. coli once the electrotransformation cuvettes arrive.

Wetlab
Maria and Afton Trentay 19:36, 6 July 2009 (UTC)
 * n/a