Virginia Commonwealth/9 July 2009

Results
Afton and Maria
 * Electrotransformation was successful

Trentay 14:06, 9 July 2009 (UTC)

Tasks
Afton and Maria Trentay 19:45, 9 July 2009 (UTC)
 * Pick cells for overnight culture

Kevin and Adam
 * Proceed with analytical work and ordering DNA.

Craig and Clay
 * The three parts for limonene synthesis (BBa_I742111, BBa_K118024, and BBa_K118025) will be electrotransformed today. They will also be plated and grown overnight on ampicillin-selective media.
 * Tomorrow colonies will be selected and grown in ampicillin-selective liquid media during the day. Later in the evening a miniprep will be performed on the grown parts.

Wetlab
Afton and Maria Trentay 21:13, 9 July 2009 (UTC)
 * Put 5 colonies of J23106 in 5mL of LB (two test tube)
 * Put 5 colonies of J06702 in 5mL of LB (one test tube)

Craig and Clay
 * The following parts for limonene synthesis were electrotransformed into the NEB 10 beta strain of E coli: BBa_I742111, BBa_K118024, and BBa_K118025. All DNA was obtained from the distribution plates.  These electrotransformed parts were then plated on ampicillin-selective plates and left to grow overnight.