Virginia Commonwealth/18 August 2009

Results
Maria and Afton Trentay 14:11, 18 August 2009 (UTC)
 * Re-Inoculated cultures with 10µ sample in 5mL of LB+Amp show no expression
 * Overnight cultures made Sunday: only J23116 is expressing florescence (slightly pink)
 * Looking over the past results of transformations on June 11:
 * Only J23100-104 expressed florescence on the plates
 * The overnight cultures say, "all cells grew well"
 * It is not 100% clear whether ALL of the cells with promoter parts ever expressed RFP
 * The amount of time it takes for the cells to fluoresce will be monitored from now on
 * Electrocompetency was completed
 * 80 vials of 250µL Electrocompetent NEB10β was made
 * They will be tested today to ensure the procedure was successful

Tasks
Kevin
 * PCR

Maria and Afton
 * Do Spectrophotometry of E0240 and pSB1C3
 * Digest E0240 and pSB1C3 w/p1010
 * Run a gel on E0240 and pSB1C3 w/p1010
 * Ligate E0240 w/ pSB1C3 with J23100-104
 * Digests from last week will be used for ligation
 * However, I would prefer to transform Ligations with J06702 and see how well those grow before proceeding to further ligations

Trentay 14:20, 18 August 2009 (UTC)
 * Transform Ligations with pSB1C3 and J06702 with: J23100-J23107, R0011, R0040
 * Transform newly made electrocompetent cells to make sure they are working

Wetlab
Maria and Afton Trentay 02:25, 19 August 2009 (UTC)
 * Transformations were done