Virginia Commonwealth/26 May 2009

Results

 * Overnight cultures grew well.

Tasks

 * Inoculate 2.5 mL from each starter culture into 250mL LB media in 500ml flasks. Culture until ~0.6-1.0 A600.
 * Freeze some glycerol seed stocks of NEB10beta and DB3.1 (2 each), concentrate rest 100x and do cold glycerol washes to make competent for electrotransformation.
 * Freeze stocks of electrocompetent cells (-80C freezer in George's E. coli box).

Wetlab

 * n/a