Virginia Commonwealth/21 May 2009

Tasks

 * 1) Make stock of electrocompetent E. coli K-12
 * 2) Transform E. coli with the following:
 * 3) *BBa_I13521 (RPF PRET) - located in plate 2, well 6O AmpR
 * 4) *BBa_I13522 (GRP PRET) located in plate 2, well 8A AmpR
 * 5) *BBa_E0840 (GFP RET) - located in plate 1, well 12O AmpR
 * 6) *BBa_R0040 (Promoter) - located in plate 1, well 6I AmpR
 * 7) *pSB1AK3-J04450 (KanR plasmid w/ RPF insert) - located in plate 1, well 13A KanR
 * 8) *BBa_E0240 (GFP RET) - located in plate 1, well 12M AmpR
 * 9) *BBa_J23101 (Promoter) - located in plate 1, well 18E AmpR
 * 10) *BBa_I20260 (Standard promoter) - located in plate 2, well 17F KanR
 * 11) Plate cells on LB with appropriate antibiotic - grow overnight

Results

 * 1) Plates were prepared for today's work.