Template:Team:KULeuven/26 August 2009/VanillinProduction

Nanodrop results:
 * Miniprep of fluid cultures

-We performed 2 simultaneous digests: one to verify the length of the product (Restriction2Test) using SAMS II and EF III and another to prepare for ligation (Restriction4Real) with promotor and terminator using SAMS III and EF II
 * Restriction:

-We cut with the 2 enzymes separately, allowing a one hour incubation in between. EF: E/S SAMS: E/S

-Restriction volumes:

Restriction2Test

Restriction4Real

Terminator: E/X
 * Terminator was also digested


 * Restriction2Test: Agarose gel electrophoresis to validate if the test samples have the correct length. Signals appeared that could not be explained