Team:UNICAMP-Brazil/Notebooks/September 1

Gallus gallus cDNA synthesis

 * We decided to make cDNA synthesis of the 4 samples of RNA (Blood1, Blood3, Spleen1 and Oviduct2). We used both pDN6 and oligodT primers for the synthesis.

Taís

RBS+CFP Digestion

 * We performed a new digestion of the plasmids containing the ligation RBS+CFP. We followed Protocol 14. The digestion lasted four hours and we used less plasmid comparing to the first digestion.

Wesley