Virginia Commonwealth/7 August 2009

Results
Trentay 23:49, 8 August 2009 (UTC)
 * Overnight Culture of psB1C3, I1352, and pSB4C5 was successful.
 * I1352 showed pink florescence

Tasks
Trentay 20:58, 6 August 2009 (UTC)
 * Plan for the next few Days

Maria and Afton Trentay 23:49, 8 August 2009 (UTC)
 * Make Cryogenic stocks of overnight cultures (2 vials each)
 * Miniprep two vials of pSB1C3 w/ P1010, and one vial of I1352 and pSB4C5
 * Run a Gel on the Miniprepped DNA
 * Gels are run at 100 volts for 1 hour
 * Take Spectrophotometry measurements
 * Digest parts
 * Run a Gel on Digested DNA
 * Ligate pSB1C3 with I1352 and I1352 with itself
 * Transform ligations into NEB10β
 * This was done, because if the Death gene P1010 went back into the 1C3 backbone, the cell would die, so only desirable parts would grown on an LB plate

Wetlab
-Bussingkm 22:42, 7 August 2009 (UTC)
 * 1 Cell stock of parts BBa_I13600, BBa_K118024, BBa_K118025, BBa_I742111 was made and stored in 15% glycerol in the -80C freezer.
 * 2 The same parts were minipreped using the standard protocol

Maria and Afton Trentay 23:57, 8 August 2009 (UTC)
 * Cryogenic stocks were made
 * Miniprep was done
 * Gel was run for miniprepped data
 * Digest was done
 * Gel was run for digested data
 * Gel will need to be redone because the wrong ladder was used, so bands could not be compared to the ladder
 * Ligations were done
 * Transformations were done