Team:Todai-Tokyo/Protocols/Miniprep

Miniprep Protocol
Preparation (previous night)


 * 1) Pick Single colonies from a transformation plate and culture in 4ml Luria Broth
 * 2) Culture overnight at 37ºC with vigorous shaking

Miniprep


 * 1) Aliquot 2ml of the bacterial suspension from above into a 2ml eppendorf
 * 2) Spin down for 5 min. at max speed to pellet cells
 * 3) Use the Promega miniprep kit according to instructions

We usually elute the plasmid DNA from the spin column by 50µl MilliQ and measure nucleotide concentration on a spectrophotometer to label on the tube.