Week5



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1.FACS run with different conc. of IPTG (100,250,500 microM/ml) for CFP and YFP-no signal(reason: sample stored after pelleting)

2.FACS run with different conc. of IPTG (100,250,500 microM/ml) for CFP and YFP-

signal obtained for YFP with gradation as per IPTG conc.

no signal obtained for CFP


 * CFP culture problem suspected