Team:Warsaw/Calendar-Main/8 August 2009

Assembly of endosomal detection operon Marcin Task 1:  Assembly of  BBa_B0032 - cro construct  Gel electrophoresis and subsequent purification of digested products via gel-out   Evaluation of results of the digestion  Procedure:  Fragments of agarose gel were carefully cut out and in the next step DNA was extracted from the gel using the A&A gel-out kit. Detailed procedure is described here</li></ul> Task 2:  Cloning cro coding sequence to  pSB1A3</a> vector</li> </ul> Methods:  Ligation mixture composition: 16 &mu;l digested cro 10 &mu;l digested vector 3 &mu;l ligation buffer (Fermentas) 1 &mu;l ligase T4 (Fermentas) </li> Duration of ligation was about 18 hours; reaction was conducted in 19 &deg;c (approximately).</li> </ul>