Wisconsin-Madison/3 October 2009

October 3, 2009
Cloning

'''50uL / DNA / Buffer / H2O / Enzyme

ProU / 40 / 5 / 4 / EcoR I 1 SpeI 1.5

metK / 40 / 5 / 4 / EcoR I 1 PstI 1.5

pBAD35 / 40 / 5 / 4 / EcoR I 1 PstI 1.5

BTE / 40 / 5 / 4 / EcoR I 1 PstI 1.5

RFP / 40 / 5 / 4 / EcoR I 1 XbaI 1.5

Lac / 40 / 5 / 4 / EcoR I 1 SpeI 1.5

Hawaii / 40 / 5 / 4 / EcoR I 1 PstI 1.5

Photo system / 40 / 5 / 4 / EcoR I 1 PstI 1.5

PCR clean-up

Run gel

Ligation