Team:Warsaw/Calendar-Main/21 August 2009

Assembly of endosomal detection operon Marcin Task 1: Prepare the bacterial cultures for isolation of following constructs:  p53 CDS +  BBa_B0032 on  pSB1A3 plasmid cro CDS +  pSB1A3 plasmid Digest of isolated plasmids and transformation of chemocompetent E. coli strain DH5&alpha with  BBa_C0040</a> +  BBa_R0080</a> on  pSB1A3</a> were done by Kuba

Making of the plac-RBS-llo part Jarek

Tasks:  Separation of digestion products from 20 August in 0,8% agarose gel. </li> Digestion of 8 plasmid DNA samples with XbaI and PstI endonucleases.</li> Separation of digestion products in 0,8% agarose gel.</li> Transformation competent E.coli strain with DNA sample containing plac+RBS+llo part and plating the transformated culture on the LB+Amp plates. </li> </ul>

Cloning switch 1 regulatory parts [ K177012</a>-PcI.RBS.LacI, K177033</a>-PcI.RBS.LacI.PcI.RBS.RFP.terminator, K177011</a>-PLacI.RBS.cI.terminator, K177038</a>-PLacI.RBS.cI.terminator.PLacI.RBS.GFP.terminator ] into two compatible low copy number plasmids of different antibiotic resistance Ania Tasks:   </li> </ul>