Team:SDU-Denmark/Protocols/Primer-PCR

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=Protocol for Primer PCR=

dNTP mix (10mM)
10ul 100mM dGTP

10ul 100mM dATP

10ul 100mM dTTP

10ul 100mM dCTP

60ul H2O

(Total V=100ul)

PCR reaction
39ul H2O

5.0ul Pfx-buffer (÷MgSO4)

1.0ul 50mM MgSO4

1.5ul 10mM dNTP mix

1.5ul forward primer

1.5ul reverse primer

0.5ul Pfx enzyme (add ju1st before PCR run)