Team:Brown/Notebook Recipes/TSBPlates

Making Tyrpticase Soy Broth (TSB) Plates (For 1L = ~ 30plates)

1.	Add 30g of TSB into 2.	1 L ddH2O in 2L of Flask 3	Swirl the flask until all the LB is dissolved. 4.	Add 15g Agar 5	Bring the solution to a boil (Clear) on a hotplate with a stir bar stirring. (Temperature ~ 430 oC) 6	Autoclave the solution at 20min at 15psi and 121 oC. 7	Cool the temperature down to 50 oC - 60 oC OR cool to the touch. 8.	Add the selected antibiotic (the right concentration and the right volume) o	Ampicillin (Amp) = 1 ml of [50ug/ml] o	Kanamycin (Kan) = 1 ml of [50mg/ml] o	Tetracycline (Tet) = 1 ml of [12.50mg/ml] o	Chloramphenicol (Ch) = 1ml of [10mg/ml] 9.	Swirl the solution slowly to mix the antibiotics without forming a lot of bubbles. 10.	Pour the medium into the dishes as fast as you can without forming bubbles. o	Stack the plates on top of each other (~ 5 plates a stack) to lessen the chance of wetting the plates due to condensation.