Wisconsin-Madison/19 May 2009

May 19, 2009
 Chemical Safety Training: [ChemTraining]

 Biological Satety Training: [BioTraining]

Ex 1: 	YhfR grew on Amp resistance plate

NudF grew on Amp resistance plate

Made liquid cultures of NudF and YhfR

Ex 2: Plated plasmids obtained from Dan;

1.	pBAD33 –Cm resistance

2.	pTrc 99A – Amp resistance

3.	pBt2BAD18 – Kan resistance

- inoculated pNudF and pYhfR liquid cultures in LB media for overnight growth - NP

- obtained plasmids pBAD33 (cm), pTrc99A (amp), and pBT2bad18 (kan) from Dan Agnew and grew on appropriate plates overnight - NP