Virginia Commonwealth/6 August 2009

Results
Kevin -Bussingkm 00:21, 7 August 2009 (UTC)
 * Everything that I digested, ligated, and transformed yesterday grew over night. However, I used the wrong plasmid backbone.  Both my reporter and backbone insert reported RFP.  Will be growing same backbone containing the death gene (BBa_P1010).  We will use E. coli strain Db3.1 to grow plasmid.  The DNA will be minipreped Friday and digested and ligated on Monday.

Maria and Afton Trentay 01:12, 7 August 2009 (UTC)
 * Overnight culture was unsuccessful because not all of the parts were prepared for an overnight culture. Only pSB1C3 was picked from a plate. The other parts, J06702 and a promoter were not grown up so all parts will be grown up today.

Tasks
Kevin -Bussingkm 00:21, 7 August 2009 (UTC)
 * I need to finish writing a progress report for my fellowship. Hopefully the flow cytometer will be fixed soon so that we can finally get some results.  Confirmed that whenever we have florescing bacteria ready we can proceed with RT PCR.

Maria and Afton Trentay 01:12, 7 August 2009 (UTC)
 * Make overnight culture of J06702 and J23100/110 from frozen stocks
 * Pick colony from pSB1C3 for overnight culture

Wetlab
All parts came on the high copy, ampicillin resistant backbone pSB1A2
 * Picked colonies for several new plasmids that came in today. The plasmids contained parts:
 * BBa_I13600  tetR promoter with CFP RET
 * BBa_K118024 Craig and Clay's pathway sequence
 * BBa_K118025
 * BBa_I742111

-Bussingkm 00:21, 7 August 2009 (UTC)

Maria and Afton Trentay 01:12, 7 August 2009 (UTC)
 * Overnight cultures were made