September/10 September 2009

1.colony check ( sample No.) (no. of colonies) 24               7       24                0       29                1       30                0       30                0       33               100<       34                50<       35                2       36                0       37                0        38                0       39                1       40               10

2.transformation ligation sample No. 41, 42 , 14 , 22 3-1M 37degree 10hr

3.Min prep Result of Nanodrop concentration check (sample No.) (concentration) 9A            16.3 B            13.4 C            21.8 D            15.3 31A           17.4 B           14.3 C           15.3 32A           14.5 B           11.9

4.digestion and ligation

digestion with restriction enzyme

K204043 : 1-16I

K204044  :1-1K

↓ gel purification ↓ ligation 37degree O/N

5.ligation check UP  : 10-D, 10-E , 9-A , 9-B , 9-C , 9-D , ladder DOWN : 31-A, 31-B , 31-C , 32-A , 32-B , ladder

No.9 OK!

6.transformation ligation sample No. 24, 29 , 30 , 36 , 37 , 38 , 43 , 44

7.colony check ( sample No.) (no. of colonies) 3-1M            0 14            +++        22             +++        41             +++        42             ++

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