Duke University/4 June 2009

Return

June 4, 2009
E: Gel to check Bioplastics #1-5 R: All correct sized bands C: Use one as template for PCR

E: PCR with Bioplastics #5 as template

5x HF buffer 5 ul dNTPs 2 ul forward primer 1.25 ul reverse primer 1.25 ul DNA template (plasmid) 0.5 ul Phusion DNA polymerase 0.3 ul H2O 14.7 ul 25 ul

98°C 30s 1x 98°C 15s 55°C 30s 72°C 1m 10s 35x 72°C 5m 4°C infinity 1x

Fragment # Name Size/Length 1 pASKphaA 4.2 kb 2 phaB 900 bp 3 Termcat2 1.7 kb 4 phaC 1.9 kb