LOCUS Strep+Dig+longli 3637 bp DNA circular SYN 08-OCT-2009 DEFINITION Ligation of Strep+Dig+longlinker+FokA into pMA2_NgoMIV_SpeI ACCESSION Strep+Dig+longli KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 3637) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by SciEd Central, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: pMA2_NgoMIV_SpeI Fragment ends: SpeI and NgoMIV Fragment size: 2412 Insert molecule: Strep+Dig+longlinker+FokA Fragment ends: NgoMIV and SpeI Fragment size: 1225 FEATURES Location/Qualifiers CDS 129..989 /gene="AmpR" /SECDrawAs="Gene" CDS 2014..3234 /gene="'ORF-1" /SECDrawAs="Gene" CDS 2014..2041 /gene="'Strep-Tag'" /SECDrawAs="Gene" CDS 2044..2599 /gene="'Dig'" /SECDrawAs="Gene" CDS 2602..2641 /gene="'LongLinker'" /SECDrawAs="Gene" CDS 2644..3231 /gene="'FokA" /SECDrawAs="Gene" ORIGIN 1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 841 gtggttctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc 1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 1801 ctcattaggc accccaggct ttacccgaac gaccgagcgc agcgagtcag tgagcgagga 1861 agcggagagc gcccaatacg caaggaaaca gctatgacca tgttaatgca gctggcacga 1921 caggtttccc gactggaaag cgggcagtga gcggaaggcc catgaggcca gttaattaag 1981 aggtaccgaa ttcgcggccg cttctagatg gccggctgga gccatccgca gtttgaaaaa 2041 accggcgacg tgtaccacga cggcgcctgc cccgaagtga agcccgtgga caacttcgac 2101 tggtcccagt accacggcaa gtggtggcag gtggccgctt atcccgacca catcaccaag 2161 tacggcaagt gcggctgggc cgagtacacc cccgagggca agagcgtgaa ggtgtcccgg 2221 tacagcgtga tccacggcaa agagtacttc agcgagggca ccgcctaccc tgtgggcgac 2281 agcaagatcg gcaagatcta ccacagctac accatcggcg gcgtgaccca ggtgggcgtg 2341 agcaacgtgc tgtccaccga caacaagaac tacatcatcg gctacttttg cagatacgac 2401 gaggacaaga agggccactg ggacgccgtg tgggtgctgt cccggtccat ggtgctgacc 2461 ggcgaggcca agaccgccgt ggagaactac ctgatcggca gccccgtggt ggacagccag 2521 aaactggtgt acagcgactt ctccgaggcc gcctgcaaag tgaacaacag caactggtcc 2581 cacccccagt tcgaaaagac cggcggtggt tctggtggtg gttctggtgg tggttctggt 2641 accggcaaat ctgaactgga ggagaaaaaa tccgagctgc gccacaaact gaaatatgtg 2701 cctcacgagt atatcgaact gatcgagatc gcccgtaata gtacccaaga ccgtatcctg 2761 gaaatgaaag tgatggagtt cttcatgaaa gtctatggct atcgtggcaa acatctgggt 2821 ggtagccgta aacctgatgg tgccatttat accgttggtt ccccgatcga ttatggcgtt 2881 atcgttgata ccaaagccta tagcgggggt tataacctgc caattggtca ggctgatgag 2941 atgcagcgtt atgtgaaaga gaaccagact cgtaacaaac acatcaaccc gaacgaatgg 3001 tggaaagtgt atccgtcaag cgttacagag ttcaaattcc tgttcgtgag cggccatttt 3061 aaaggcaact ataaagcaca gctgacccgt ctgaaccata aaaccaatag caatggcgcc 3121 gttctgtcag tagaagagct gctgattggc ggtgaaatga tcaaagccgg gaccctgaca 3181 ctggaagaag ttcgccgtaa attcaacaat ggggagatca attttaccgg ttaatactag 3241 tagcggccgc tgcagagctc atggcgcgcc taggccttga cggccttccg ccaattcgcc 3301 ctatagtgag tcgtattacg tcgcgctcac tggccgtcgt tttacaacgt cgtgactggg 3361 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc 3421 gtaatagcga agaggcccgc accgaaacgc ccttcccaac agttgcgcag cctgaatggc 3481 gaatgggagc gccctgtagc ggccactcaa ccctatctcg gtctattctt ttgatttata 3541 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 3601 cgcgaatttt aacaaaatat taacgcttac aatttag //