LOCUS       ShortLinker26+Fo        3027 bp    DNA     circular SYN 20-OCT-2009
DEFINITION  Ligation of NgoMIV_FokA_PstI into shortlLinker26_pMA_PstI_AgeI
ACCESSION   ShortLinker26+Fo
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  shortlLinker26_pMA_PstI_AgeI
               Fragment ends: PstI and AgeI
               Fragment size: 2413
            Insert molecule:  NgoMIV_FokA_PstI
               Fragment ends: NgoMIV and PstI
               Fragment size: 614
FEATURES             Location/Qualifiers
     CDS             27..47
                     /gene="shortLinker26"
                     /SECDrawAs="Gene"
     CDS             27..641
                     /gene="ORF-1"
                     /SECDrawAs="Gene"
     CDS             51..638
                     /gene="'FokA"
                     /SECDrawAs="Gene"
     CDS             complement (1662..2522)
                     /gene="AmpR"
                     /SECDrawAs="Gene"
ORIGIN      
        1 gagctcgaat tcgcggccgc ttctagatgg ccggcggtgg ttctggtacc ggcaaatctg
       61 aactggagga gaaaaaatcc gagctgcgcc acaaactgaa atatgtgcct cacgagtata
      121 tcgaactgat cgagatcgcc cgtaatagta cccaagaccg tatcctggaa atgaaagtga
      181 tggagttctt catgaaagtc tatggctatc gtggcaaaca tctgggtggt agccgtaaac
      241 ctgatggtgc catttatacc gttggttccc cgatcgatta tggcgttatc gttgatacca
      301 aagcctatag cgggggttat aacctgccaa ttggtcaggc tgatgagatg cagcgttatg
      361 tgaaagagaa ccagactcgt aacaaacaca tcaacccgaa cgaatggtgg aaagtgtatc
      421 cgtcaagcgt tacagagttc aaattcctgt tcgtgagcgg ccattttaaa ggcaactata
      481 aagcacagct gacccgtctg aaccataaaa ccaatagcaa tggcgccgtt ctgtcagtag
      541 aagagctgct gattggcggt gaaatgatca aagccgggac cctgacactg gaagaagttc
      601 gccgtaaatt caacaatggg gagatcaatt ttaccggtta atactagtag cggccgcctg
      661 cagggtacct cttaattaac tggcctcatg ggccttccgc tcactgcccg ctttccagtc
      721 gggaaacctg tcgtgccagc tgcattaaca tggtcatagc tgtttccttg cgtattgggc
      781 gctctccgct tcctcgctca ctgactcgct gcgctcggtc gttcgggtaa agcctggggt
      841 gcctaatgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      901 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      961 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     1021 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     1081 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     1141 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     1201 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     1261 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1321 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     1381 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1441 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1501 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     1561 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     1621 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     1681 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1741 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1801 cgagaaccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1861 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     1921 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     1981 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     2041 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     2101 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     2161 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     2221 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     2281 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     2341 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     2401 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     2461 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     2521 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     2581 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     2641 aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     2701 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     2761 aatagaccga gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg
     2821 cgcaactgtt gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     2881 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     2941 ttgtaaaacg acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg
     3001 aaggccgtca aggcctaggc gcgccat
//