LOCUS DsbA+His+Dig+Spl 3700 bp DNA circular SYN 08-OCT-2009 DEFINITION Ligation of inverted DsbA+His+Dig+Split+FokA into pMA_cut ACCESSION DsbA+His+Dig+Spl KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 3700) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by SciEd Central, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: pMA_cut Fragment ends: XbaI and AgeI Fragment size: 2414 Insert molecule: DsbA+His+Dig+Split+FokA Fragment ends: XbaI and AgeI Fragment size: 1286 FEATURES Location/Qualifiers CDS complement (412..994) /gene="'Fok active''" /SECDrawAs="Gene" CDS complement (998..1048) /gene="Split-Linker" /SECDrawAs="Gene" CDS complement (1054..1609) /gene="'Dig'" /SECDrawAs="Gene" CDS complement (1613..1630) /gene="His Tag" /SECDrawAs="Gene" CDS complement (1635..1691) /gene="DsbA" /SECDrawAs="Gene" CDS complement (404..1693) /gene="ORF-1" /SECDrawAs="Gene" CDS complement (2712..3572) /gene="AmpR" /SECDrawAs="Gene" ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt 181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca 361 aggcctaggc gcgccatgag ctctgcagcg gccgctacta gtattaaccg gtaaaattga 421 tctccccatt gttgaattta cggcgaactt cttccagtgt cagggtcccg gctttgatca 481 tttcaccgcc aatcagcagc tcttctactg acagaacggc gccattgcta ttggttttat 541 ggttcagacg ggtcagctgt gctttatagt tgcctttaaa atggccgctc acgaacagga 601 atttgaactc tgtaacgctt gacggataca ctttccacca ttcgttcggg ttgatgtgtt 661 tgttacgagt ctggttctct ttcacataac gctgcatctc atcagcctga ccaattggca 721 ggttataacc cccgctatag gctttggtat caacgataac gccataatcg atcggggaac 781 caacggtata aatggcacca tcaggtttac ggctaccacc cagatgtttg ccacgatagc 841 catagacttt catgaagaac tccatcactt tcatttccag gatacggtct tgggtactat 901 tacgggcgat ctcgatcagt tcgatatact cgtgaggcac atatttcagt ttgtggcgca 961 gctcggattt tttctcctcc agttcagatt tgccggtgtg attcataact ttctgcttca 1021 ggtcatttgg aatcttacag gctggtcggc cggtcttttc gaactggggg tgggaccagt 1081 tgctgttgtt cactttgcag gcggcctcgg agaagtcgct gtacaccagt ttctggctgt 1141 ccaccacggg gctgccgatc aggtagttct ccacggcggt cttggcctcg ccggtcagca 1201 ccatggaccg ggacagcacc cacacggcgt cccagtggcc cttcttgtcc tcgtcgtatc 1261 tgcaaaagta gccgatgatg tagttcttgt tgtcggtgga cagcacgttg ctcacgccca 1321 cctgggtcac gccgccgatg gtgtagctgt ggtagatctt gccgatcttg ctgtcgccca 1381 cagggtaggc ggtgccctcg ctgaagtact ctttgccgtg gatcacgctg taccgggaca 1441 ccttcacgct cttgccctcg ggggtgtact cggcccagcc gcacttgccg tacttggtga 1501 tgtggtcggg ataagcggcc acctgccacc acttgccgtg gtactgggac cagtcgaagt 1561 tgtccacggg cttcacttcg gggcaggcgc cgtcgtggta cacgtcgccg gtatgatgat 1621 gatgatgatg gccggtcgcc gatgcgctaa acgctaaaac taaaccagcc agcgccagcc 1681 aaatcttttt catctagaag cggccgcgaa ttcggtacct cttaattaac tggcctcatg 1741 ggccttccgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaaca 1801 tggtcatagc tgtttccttg cgtattgggc gctctccgct tcctcgctca ctgactcgct 1861 gcgctcggtc gttcgggtaa agcctggggt gcctaatgag caaaaggcca gcaaaaggcc 1921 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 1981 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 2041 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 2101 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 2161 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 2221 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 2281 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 2341 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 2401 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 2461 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 2521 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 2581 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 2641 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 2701 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 2761 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 2821 ccatctggcc ccagtgctgc aatgataccg cgagaaccac gctcaccggc tccagattta 2881 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 2941 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 3001 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 3061 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 3121 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 3181 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 3241 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 3301 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 3361 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 3421 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 3481 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 3541 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 3601 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 3661 caaatagggg ttccgcgcac atttccccga aaagtgccac //