RNA - DNA Report

Report time                      2009-09-04 14:37:35                                  
Batch name                       C:\Documents and Settings\Ellenor                    
                                 Olsson\Desktop\iGEM\Plasmidprep_090904.BDN           
Application                      RNA - DNA Estimation 3.00(184)                       
Operator                                                                              

Instrument Settings
Instrument                       Cary 50                                              
Instrument version no.           3,00                                                 
Ordinate Mode                    Abs                                                  
Ave Time (sec)                   1,0000                                               
Replicates                       3                                                    
Wavelength 1 (nm)                260,0                                                
Wavelength 2 (nm)                280,0                                                
Background Correction            ON                                                   
Background Wavelength (nm)       320,0                                                
Protein Factor for 260,0 (P1)    757,3000                                             
Protein Factor for 280,0 (P2)    1552,0000                                            
Nucleic Factor for 260,0 (N1)    62,9000                                              
Nucleic Factor for 280,0 (N2)    -36,0000                                             

Ratio = (A[260,0] - Bg[320,0])/(A[280,0] - Bg[320,0])
Protein Conc = ((A[280,0] - Bg[320,0]) * P2) - ((A[260,0] - Bg[320,0]) * P1)
Nucleic Acid Conc = ((A[260,0] - Bg[320,0]) * N1) + ((A[280,0] - Bg[320,0]) * N2)

Analysis
Collection time                  2009-09-04 14:37:35                                  

    Sample        A[260]     A[280]    Bg[320]     Ratio     Protein   Nucleic Acid     SD       %RSD      Mean    
                                                              µg/ml       µg/ml                                    
___________________________________________________________________________________________________________________
RBS-Adh2Y_AC3       0,0212     0,0105    -0,0002     1,9957     0,4366       0,9610                                
                    0,0217     0,0108    -0,0001     2,0050     0,3648       0,9784                                
                    0,0219     0,0109    -0,0001     1,9860     0,5314       0,9847     0,0095      0,48    1,9956