LOCUS His+Dig+Split+Fo 5011 bp DNA circular SYN 20-OCT-2009 DEFINITION Ligation of Fragment 1 into Fragment 2 ACCESSION His+Dig+Split+Fo KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 5011) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by SciEd Central, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: Fragment 2 Fragment ends: NotI and XbaI Fragment size: 3761 Insert molecule: Fragment 1 Fragment ends: XbaI and NotI Fragment size: 1250 FEATURES Location/Qualifiers CDS 74..1165 /gene="lacI reg" /product="CDS CDS Unknown feature type:Regulatory_Seq Unknown feat ure type:Regulatory_Seq Unknown feature type:Regulator y_SeqUnknown feature type:Regulatory_Seq" /SECDrawAs="Gene" CDS 1387..2622 /gene="ORF-1" /SECDrawAs="Gene" CDS 1396..1413 /gene="His Tag" /SECDrawAs="Gene" CDS 1417..1972 /gene="'Dig'" /SECDrawAs="Gene" CDS 1978..2028 /gene="Split-Linker" /SECDrawAs="Gene" CDS 2032..2624 /gene="'Fok active'" /SECDrawAs="Gene" CDS 3372..4031 /gene="CAT marker" /product="CDS CDS Unknown feature type:Marker Unknown feature type :Marker Unknown feature type:MarkerUnknown feature type:Marker" /SECDrawAs="Gene" ORIGIN 1 acccgacacc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga 61 gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc 121 cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa 181 aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc 241 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct 301 gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag 361 cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa 421 tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc 481 cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca 541 gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca 601 tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc 661 ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat 721 agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct 781 gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc 841 aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gacatctcgg tagtgggata 901 cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt 961 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt 1021 gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa 1081 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt 1141 ttcccgactg gaaagcgggc agtgagcggt acccgataaa agcggcttcc tgacaggagg 1201 ccgttttgtt ttgcagccca cctcaacgca attaatgtga gttagctcac tcattaggca 1261 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa 1321 caatttcaca caggaaacag ctatgaccat gattacgaat ttctagcata acgagggcaa 1381 atctagatgg ccggccatca tcatcatcat cataccggcg acgtgtacca cgacggcgcc 1441 tgccccgaag tgaagcccgt ggacaacttc gactggtccc agtaccacgg caagtggtgg 1501 caggtggccg cttatcccga ccacatcacc aagtacggca agtgcggctg ggccgagtac 1561 acccccgagg gcaagagcgt gaaggtgtcc cggtacagcg tgatccacgg caaagagtac 1621 ttcagcgagg gcaccgccta ccctgtgggc gacagcaaga tcggcaagat ctaccacagc 1681 tacaccatcg gcggcgtgac ccaggtgggc gtgagcaacg tgctgtccac cgacaacaag 1741 aactacatca tcggctactt ttgcagatac gacgaggaca agaagggcca ctgggacgcc 1801 gtgtgggtgc tgtcccggtc catggtgctg accggcgagg ccaagaccgc cgtggagaac 1861 tacctgatcg gcagccccgt ggtggacagc cagaaactgg tgtacagcga cttctccgag 1921 gccgcctgca aagtgaacaa cagcaactgg tcccaccccc agttcgaaaa gaccggccga 1981 ccagcctgta agattccaaa tgacctgaag cagaaagtta tgaatcacac cggcaaatct 2041 gaactggagg agaaaaaatc cgagctgcgc cacaaactga aatatgtgcc tcacgagtat 2101 atcgaactga tcgagatcgc ccgtaatagt acccaagacc gtatcctgga aatgaaagtg 2161 atggagttct tcatgaaagt ctatggctat cgtggcaaac atctgggtgg tagccgtaaa 2221 cctgatggtg ccatttatac cgttggttcc ccgatcgatt atggcgttat cgttgatacc 2281 aaagcctata gcgggggtta taacctgcca attggtcagg ctgatgagat gcagcgttat 2341 gtgaaagaga accagactcg taacaaacac atcaacccga acgaatggtg gaaagtgtat 2401 ccgtcaagcg ttacagagtt caaattcctg ttcgtgagcg gccattttaa aggcaactat 2461 aaagcacagc tgacccgtct gaaccataaa accaatagca atggcgccgt tctgtcagta 2521 gaagagctgc tgattggcgg tgaaatgatc aaagccggga ccctgacact ggaagaagtt 2581 cgccgtaaat tcaacaatgg ggagatcaat tttaccggtt aatactagta gcggccgctg 2641 cagaagcttg acctgtgaag tgaaaaatgg cgcacattgt gcgacatttt ttttgtctgc 2701 cgtttaccgc tactgcgtca cggatcccca cgcgccctgt agcggcgcat taagcgcggc 2761 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc 2821 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa 2881 tcggggcatc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact 2941 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt 3001 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa 3061 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt 3121 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac 3181 aatttcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 3241 aatacattca aatatgtatc cgctcatgtc gagacgttgg gtgaggttcc aactttcacc 3301 ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta 3361 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc 3421 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg 3481 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc 3541 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggagttc cgtatggcaa 3601 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg 3661 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc 3721 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag 3781 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg 3841 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt 3901 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtctgtg 3961 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg 4021 gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt gctacgcctg 4081 aataagtgat aataagcgga tgaatggcag aaattcgaaa gcaaattcga cccggtcgtc 4141 ggttcagggc agggtcgtta aatagccgct tatgtctatt gctggtttac cggtttattg 4201 actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct gaggccagtt tgctcaggct 4261 ctccccgtgg aggtaataat tgctcgacat gaccaaaatc ccttaacgtg agttttcgtt 4321 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 4381 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 4441 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 4501 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 4561 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 4621 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 4681 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 4741 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 4801 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 4861 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 4921 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 4981 cctggccttt tgctggcctt ttgctcacat g //