LOCUS middelLinker+Fok 3039 bp DNA circular SYN 20-OCT-2009 DEFINITION Ligation of NgoMIV_FokA_PstI into PstI_MiddelLinker_AgeI ACCESSION middelLinker+Fok KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 3039) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by SciEd Central, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: PstI_MiddelLinker_AgeI Fragment ends: PstI and AgeI Fragment size: 2425 Insert molecule: NgoMIV_FokA_PstI Fragment ends: NgoMIV and PstI Fragment size: 614 FEATURES Location/Qualifiers CDS 27..59 /gene="middelLinker38" /SECDrawAs="Gene" CDS 27..653 /gene="ORF-1" /SECDrawAs="Gene" CDS 63..650 /gene="'FokA" /SECDrawAs="Gene" CDS complement (1674..2534) /gene="AmpR" /SECDrawAs="Gene" ORIGIN 1 gagctcgaat tcgcggccgc ttctagatgg ccggcggtgg ttctggtggt ggttctggta 61 ccggcaaatc tgaactggag gagaaaaaat ccgagctgcg ccacaaactg aaatatgtgc 121 ctcacgagta tatcgaactg atcgagatcg cccgtaatag tacccaagac cgtatcctgg 181 aaatgaaagt gatggagttc ttcatgaaag tctatggcta tcgtggcaaa catctgggtg 241 gtagccgtaa acctgatggt gccatttata ccgttggttc cccgatcgat tatggcgtta 301 tcgttgatac caaagcctat agcgggggtt ataacctgcc aattggtcag gctgatgaga 361 tgcagcgtta tgtgaaagag aaccagactc gtaacaaaca catcaacccg aacgaatggt 421 ggaaagtgta tccgtcaagc gttacagagt tcaaattcct gttcgtgagc ggccatttta 481 aaggcaacta taaagcacag ctgacccgtc tgaaccataa aaccaatagc aatggcgccg 541 ttctgtcagt agaagagctg ctgattggcg gtgaaatgat caaagccggg accctgacac 601 tggaagaagt tcgccgtaaa ttcaacaatg gggagatcaa ttttaccggt taatactagt 661 agcggccgcc tgcagggtac ctcttaatta actggcctca tgggccttcc gctcactgcc 721 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa catggtcata gctgtttcct 781 tgcgtattgg gcgctctccg cttcctcgct cactgactcg ctgcgctcgg tcgttcgggt 841 aaagcctggg gtgcctaatg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 901 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 961 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 1021 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 1081 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 1141 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 1201 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 1261 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 1321 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg 1381 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 1441 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 1501 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 1561 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 1621 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 1681 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 1741 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 1801 gcaatgatac cgcgagaacc acgctcaccg gctccagatt tatcagcaat aaaccagcca 1861 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 1921 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 1981 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 2041 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 2101 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 2161 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 2221 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 2281 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 2341 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 2401 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 2461 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 2521 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 2581 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 2641 acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt 2701 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt 2761 ataaatcaaa agaatagacc gagatagggt tgagtggccg ctacagggcg ctcccattcg 2821 ccattcaggc tgcgcaactg ttgggaaggg cgtttcggtg cgggcctctt cgctattacg 2881 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc 2941 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgac gtaatacgac tcactatagg 3001 gcgaattggc ggaaggccgt caaggcctag gcgcgccat //