LOCUS DsbA+Strep+Dig+S 5765 bp DNA circular SYN 20-OCT-2009 DEFINITION Ligation of XbaI_DsbA_AgeI into Strep+Dig+Split+FokA_pEX ACCESSION DsbA+Strep+Dig+S KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 5765) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by SciEd Central, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: Strep+Dig+Split+FokA_pEX Fragment ends: NgoMIV and XbaI Fragment size: 5703 Insert molecule: XbaI_DsbA_AgeI Fragment ends: XbaI and AgeI Fragment size: 62 FEATURES Location/Qualifiers CDS 1528..2823 /gene="ORF-1" /SECDrawAs="Gene" CDS 1530..1585 /gene="DsbA" /SECDrawAs="Gene" CDS 1588..1615 /gene="'Strep-Tag'" /SECDrawAs="Gene" CDS 1618..2173 /gene="'Dig'" /SECDrawAs="Gene" CDS 2179..2229 /gene="Split-Linker" /SECDrawAs="Gene" CDS 2233..2825 /gene="'Fok active'" /SECDrawAs="Gene" CDS 3175..4035 /gene="AmpR" /SECDrawAs="Gene" ORIGIN 1 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga 61 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg 121 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa 181 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac 241 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc 301 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg 361 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc 421 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca 481 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga 541 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc 601 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg 661 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag 721 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga 781 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa 841 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatttcggta gtgggatacg 901 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc 961 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga 1021 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata 1081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 1141 cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag 1201 gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg 1261 tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg 1321 tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt 1381 ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga 1441 attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga 1501 gcaattgacc aacaaggaga aatctagatg aaaaagattt ggctggcgct ggctggttta 1561 gttttagcgt ttagcgcatc ggcgaccggc tggagccatc cgcagtttga aaaaaccggc 1621 gacgtgtacc acgacggcgc ctgccccgaa gtgaagcccg tggacaactt cgactggtcc 1681 cagtaccacg gcaagtggtg gcaggtggcc gcttatcccg accacatcac caagtacggc 1741 aagtgcggct gggccgagta cacccccgag ggcaagagcg tgaaggtgtc ccggtacagc 1801 gtgatccacg gcaaagagta cttcagcgag ggcaccgcct accctgtggg cgacagcaag 1861 atcggcaaga tctaccacag ctacaccatc ggcggcgtga cccaggtggg cgtgagcaac 1921 gtgctgtcca ccgacaacaa gaactacatc atcggctact tttgcagata cgacgaggac 1981 aagaagggcc actgggacgc cgtgtgggtg ctgtcccggt ccatggtgct gaccggcgag 2041 gccaagaccg ccgtggagaa ctacctgatc ggcagccccg tggtggacag ccagaaactg 2101 gtgtacagcg acttctccga ggccgcctgc aaagtgaaca acagcaactg gtcccacccc 2161 cagttcgaaa agaccggccg accagcctgt aagattccaa atgacctgaa gcagaaagtt 2221 atgaatcaca ccggcaaatc tgaactggag gagaaaaaat ccgagctgcg ccacaaactg 2281 aaatatgtgc ctcacgagta tatcgaactg atcgagatcg cccgtaatag tacccaagac 2341 cgtatcctgg aaatgaaagt gatggagttc ttcatgaaag tctatggcta tcgtggcaaa 2401 catctgggtg gtagccgtaa acctgatggt gccatttata ccgttggttc cccgatcgat 2461 tatggcgtta tcgttgatac caaagcctat agcgggggtt ataacctgcc aattggtcag 2521 gctgatgaga tgcagcgtta tgtgaaagag aaccagactc gtaacaaaca catcaacccg 2581 aacgaatggt ggaaagtgta tccgtcaagc gttacagagt tcaaattcct gttcgtgagc 2641 ggccatttta aaggcaacta taaagcacag ctgacccgtc tgaaccataa aaccaatagc 2701 aatggcgccg ttctgtcagt agaagagctg ctgattggcg gtgaaatgat caaagccggg 2761 accctgacac tggaagaagt tcgccgtaaa ttcaacaatg gggagatcaa ttttaccggt 2821 taatactagt agcggccgct gcagaagctt caaataaaac gaaaggctca gtcgaaagac 2881 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg 2941 ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg 3001 ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg 3061 tttctacaaa ctctttcggt ccgttgttta tttttctaaa tacattcaaa tatgtatccg 3121 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 3181 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 3241 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 3301 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 3361 cgtttcccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt 3421 gacgccgggc aagagcaact cggtcgccac atacactatt ctcagaatga cttggttgag 3481 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 3541 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 3601 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 3661 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 3721 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 3781 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 3841 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 3901 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 3961 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 4021 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttcctt 4081 aggactgagc gtcaaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 4141 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 4201 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 4261 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 4321 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 4381 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 4441 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 4501 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 4561 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 4621 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 4681 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 4741 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 4801 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 4861 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac 4921 gcatctgtgc ggtatttcac accgcatata aggtgcactg tgactgggtc atggctgcgc 4981 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg 5041 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat 5101 caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtgc agcgattcac 5161 agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct 5221 ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct 5281 ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc 5341 tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac 5401 aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct 5461 tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc 5521 ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac 5581 cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg 5641 ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc 5701 gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg 5761 aaatt //