RNA - DNA Report

Report time                      2009-08-31 16:59:19                                  
Batch name                       C:\Documents and Settings\Ellenor                    
                                 Olsson\Desktop\iGEM\digest_purification_measur_098   
                                 31.BDN                                               
Application                      RNA - DNA Estimation 3.00(184)                       
Operator                                                                              

Instrument Settings
Instrument                       Cary 50                                              
Instrument version no.           3,00                                                 
Ordinate Mode                    Abs                                                  
Ave Time (sec)                   1,0000                                               
Replicates                       3                                                    
Wavelength 1 (nm)                260,0                                                
Wavelength 2 (nm)                280,0                                                
Background Correction            ON                                                   
Background Wavelength (nm)       320,0                                                
Protein Factor for 260,0 (P1)    757,3000                                             
Protein Factor for 280,0 (P2)    1552,0000                                            
Nucleic Factor for 260,0 (N1)    62,9000                                              
Nucleic Factor for 280,0 (N2)    -36,0000                                             

Ratio = (A[260,0] - Bg[320,0])/(A[280,0] - Bg[320,0])
Protein Conc = ((A[280,0] - Bg[320,0]) * P2) - ((A[260,0] - Bg[320,0]) * P1)
Nucleic Acid Conc = ((A[260,0] - Bg[320,0]) * N1) + ((A[280,0] - Bg[320,0]) * N2)

Analysis
Collection time                  2009-08-31 16:59:19                                  

    Sample        A[260]     A[280]    Bg[320]     Ratio     Protein   Nucleic Acid     SD       %RSD      Mean    
                                                              µg/ml       µg/ml                                    
___________________________________________________________________________________________________________________
RBS-B0034_E+S       0,0062     0,0041    -0,0024     1,3104     3,6458       0,3024                                
                    0,0061     0,0029    -0,0016     1,7199     1,1205       0,3241                                
                    0,0064     0,0023    -0,0013     2,1098    -0,1673       0,3535     0,3997     23,33    1,7134 

Adh2Y_E+P           0,0060     0,0036    -0,0012     1,4949     2,0103       0,2778                                
                    0,0066     0,0020    -0,0024     2,0396     0,0328       0,4070                                
                    0,0068     0,0021    -0,0024     2,0201     0,1012       0,4158     0,3090     16,69    1,8515 

Adh2Y_E+X           0,0069     0,0024    -0,0025     1,9202     0,4812       0,4171                                
                    0,0076     0,0026    -0,0031     1,8816     0,7264       0,4706                                
                    0,0077     0,0035    -0,0027     1,6777     1,7442       0,4309     0,1303      7,13    1,8265 

Adh2Y-filt_E+P      0,0061     0,0025    -0,0025     1,7166     1,2608       0,3601                                
                    0,0070     0,0041    -0,0028     1,4249     3,2426       0,3677                                
                    0,0067     0,0032    -0,0018     1,7052     1,3074       0,3574     0,1652     10,23    1,6156 

Adh2Y-filt_E+X      0,0065     0,0032    -0,0023     1,5800     1,9822       0,3534                                
                    0,0064     0,0040    -0,0014     1,4391     2,4980       0,2947                                
                    0,0093     0,0039    -0,0024     1,8522     0,9420       0,5079     0,2100     12,93    1,6238