LOCUS       His+FluA_pMA            2974 bp    DNA     circular SYN 20-OCT-2009
DEFINITION  Ligation of His/pMA (geschnitten) into FluA/pMA (geschnitten)
ACCESSION   His+FluA_pMA
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  FluA/pMA (geschnitten)
               Fragment ends: NgoMIV and PstI
               Fragment size: 556
            Insert molecule:  His/pMA (geschnitten)
               Fragment ends: PstI and AgeI
               Fragment size: 2418
FEATURES             Location/Qualifiers
     CDS             500..1360
                     /gene="AmpR"
                     /SECDrawAs="Gene"
     CDS             2379..2942
                     /gene="ORF-1"
                     /SECDrawAs="Gene"
     CDS             2388..2405
                     /gene="His Tag"
                     /SECDrawAs="Gene"
     CDS             2412..2933
                     /gene="FluA"
                     /SECDrawAs="Gene"
ORIGIN      
        1 cgcctaggcc ttgacggcct tccgccaatt cgccctatag tgagtcgtat tacgtcgcgc
       61 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
      121 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgaa
      181 acgcccttcc caacagttgc gcagcctgaa tggcgaatgg gagcgccctg tagcggccac
      241 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
      301 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
      361 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
      421 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      481 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
      541 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
      601 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
      661 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
      721 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
      781 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
      841 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
      901 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
      961 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     1021 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     1081 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     1141 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     1201 agccggtgag cgtggttctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     1261 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     1321 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     1381 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     1441 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1501 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1561 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2161 ctggcctttt gctcattagg caccccaggc tttacccgaa cgaccgagcg cagcgagtca
     2221 gtgagcgagg aagcggagag cgcccaatac gcaaggaaac agctatgacc atgttaatgc
     2281 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcggaaggc ccatgaggcc
     2341 agttaattaa gaggtaccga attcgcggcc gcttctagat ggccggccat catcatcatc
     2401 atcataccgg cgacgtgtac cacgacggcg cctgccccga agtgaagccc gtggacaact
     2461 tcgactggtc ccagtaccac ggcaagtggt gggaggtggc caagtacccc agccccaacg
     2521 gcaagtatgg caagtgcggc tgggccgagt acacccccga gggcaagagc gtgaaggtgt
     2581 ccagatacga cgtgatccac ggcaaagaat acttcatgga aggcaccgcc taccccgtgg
     2641 gcgacagcaa gatcggcaag atctaccaca gccggaccgt gggcggctac accagaaaga
     2701 ccgtgttcaa cgtgctgtcc accgacaaca agaactacat catcggctac agctgccgct
     2761 acgacgagga caagaagggc cactgggacc acgtgtgggt gctgtcccgg tccatggtgc
     2821 tgaccggcga ggccaagacc gccgtggaga actacctgat cggcagcccc gtggtggaca
     2881 gccagaaact ggtgtacagc gacttcagcg aggccgcctg caaagtgaac aacaccggtt
     2941 aatactagta gcggccgctg cagagctcat ggcg
//