LOCUS Strep+FluA+longl 3607 bp DNA circular SYN 13-OCT-2009 DEFINITION Ligation of Strep+FluA+longlinker+FokA into pMA2_NgoMIV_SpeI ACCESSION Strep+FluA+longl KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 3607) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by SciEd Central, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: pMA2_NgoMIV_SpeI Fragment ends: SpeI and NgoMIV Fragment size: 2412 Insert molecule: Strep+FluA+longlinker+FokA Fragment ends: NgoMIV and SpeI Fragment size: 1195 FEATURES Location/Qualifiers CDS 129..989 /gene="AmpR" /SECDrawAs="Gene" CDS 2014..3204 /gene="'ORF-1" /SECDrawAs="Gene" CDS 2014..2041 /gene="'Strep-Tag'" /SECDrawAs="Gene" CDS 2047..2568 /gene="FluA" /SECDrawAs="Gene" CDS 2572..2611 /gene="'LongLinker'" /SECDrawAs="Gene" CDS 2614..3201 /gene="'FokA" /SECDrawAs="Gene" ORIGIN 1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 841 gtggttctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc 1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 1801 ctcattaggc accccaggct ttacccgaac gaccgagcgc agcgagtcag tgagcgagga 1861 agcggagagc gcccaatacg caaggaaaca gctatgacca tgttaatgca gctggcacga 1921 caggtttccc gactggaaag cgggcagtga gcggaaggcc catgaggcca gttaattaag 1981 aggtaccgaa ttcgcggccg cttctagatg gccggctgga gccatccgca gtttgaaaaa 2041 accggcgacg tgtaccacga cggcgcctgc cccgaagtga agcccgtgga caacttcgac 2101 tggtcccagt accacggcaa gtggtgggag gtggccaagt accccagccc caacggcaag 2161 tatggcaagt gcggctgggc cgagtacacc cccgagggca agagcgtgaa ggtgtccaga 2221 tacgacgtga tccacggcaa agaatacttc atggaaggca ccgcctaccc cgtgggcgac 2281 agcaagatcg gcaagatcta ccacagccgg accgtgggcg gctacaccag aaagaccgtg 2341 ttcaacgtgc tgtccaccga caacaagaac tacatcatcg gctacagctg ccgctacgac 2401 gaggacaaga agggccactg ggaccacgtg tgggtgctgt cccggtccat ggtgctgacc 2461 ggcgaggcca agaccgccgt ggagaactac ctgatcggca gccccgtggt ggacagccag 2521 aaactggtgt acagcgactt cagcgaggcc gcctgcaaag tgaacaacac cggcggtggt 2581 tctggtggtg gttctggtgg tggttctggt accggcaaat ctgaactgga ggagaaaaaa 2641 tccgagctgc gccacaaact gaaatatgtg cctcacgagt atatcgaact gatcgagatc 2701 gcccgtaata gtacccaaga ccgtatcctg gaaatgaaag tgatggagtt cttcatgaaa 2761 gtctatggct atcgtggcaa acatctgggt ggtagccgta aacctgatgg tgccatttat 2821 accgttggtt ccccgatcga ttatggcgtt atcgttgata ccaaagccta tagcgggggt 2881 tataacctgc caattggtca ggctgatgag atgcagcgtt atgtgaaaga gaaccagact 2941 cgtaacaaac acatcaaccc gaacgaatgg tggaaagtgt atccgtcaag cgttacagag 3001 ttcaaattcc tgttcgtgag cggccatttt aaaggcaact ataaagcaca gctgacccgt 3061 ctgaaccata aaaccaatag caatggcgcc gttctgtcag tagaagagct gctgattggc 3121 ggtgaaatga tcaaagccgg gaccctgaca ctggaagaag ttcgccgtaa attcaacaat 3181 ggggagatca attttaccgg ttaatactag tagcggccgc tgcagagctc atggcgcgcc 3241 taggccttga cggccttccg ccaattcgcc ctatagtgag tcgtattacg tcgcgctcac 3301 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc 3361 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgaaacgc 3421 ccttcccaac agttgcgcag cctgaatggc gaatgggagc gccctgtagc ggccactcaa 3481 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt 3541 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac 3601 aatttag //