LOCUS       Strep+FluA_pMA          2980 bp    DNA     circular SYN 20-OCT-2009
DEFINITION  Ligation of Strep/pMA (geschnitten) into FluA/pMA (geschnitten)
ACCESSION   Strep+FluA_pMA
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by SciEd Central, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  FluA/pMA (geschnitten)
               Fragment ends: NgoMIV and PstI
               Fragment size: 556
            Insert molecule:  Strep/pMA (geschnitten)
               Fragment ends: PstI and AgeI
               Fragment size: 2424
FEATURES             Location/Qualifiers
     CDS             540..1400
                     /gene="AmpR"
                     /SECDrawAs="Gene"
     CDS             2419..8
                     /gene="ORF-1"
                     /SECDrawAs="Gene"
     CDS             2425..2452
                     /gene="'Strep-Tag'"
                     /SECDrawAs="Gene"
     CDS             2458..2979
                     /gene="FluA"
                     /SECDrawAs="Gene"
ORIGIN      
        1 ccggttaata ctagtagcgg ccgctgcaga gctcatggcg cgcctaggcc ttgacggcct
       61 tccgccaatt cgccctatag tgagtcgtat tacgtcgcgc tcactggccg tcgttttaca
      121 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
      181 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgaa acgcccttcc caacagttgc
      241 gcagcctgaa tggcgaatgg gagcgccctg tagcggccac tcaaccctat ctcggtctat
      301 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
      361 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
      421 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
      481 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
      541 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
      601 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
      661 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
      721 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
      781 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
      841 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
      901 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
      961 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     1021 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     1081 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     1141 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     1201 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtggttctc
     1261 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     1321 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     1381 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     1441 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     1501 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     1561 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     1621 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     1681 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     1741 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     1801 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     1981 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     2041 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     2101 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     2161 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcattagg
     2221 caccccaggc tttacccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggagag
     2281 cgcccaatac gcaaggaaac agctatgacc atgttaatgc agctggcacg acaggtttcc
     2341 cgactggaaa gcgggcagtg agcggaaggc ccatgaggcc agttaattaa gaggtaccga
     2401 attcgcggcc gcttctagat ggccggctgg agccatccgc agtttgaaaa aaccggcgac
     2461 gtgtaccacg acggcgcctg ccccgaagtg aagcccgtgg acaacttcga ctggtcccag
     2521 taccacggca agtggtggga ggtggccaag taccccagcc ccaacggcaa gtatggcaag
     2581 tgcggctggg ccgagtacac ccccgagggc aagagcgtga aggtgtccag atacgacgtg
     2641 atccacggca aagaatactt catggaaggc accgcctacc ccgtgggcga cagcaagatc
     2701 ggcaagatct accacagccg gaccgtgggc ggctacacca gaaagaccgt gttcaacgtg
     2761 ctgtccaccg acaacaagaa ctacatcatc ggctacagct gccgctacga cgaggacaag
     2821 aagggccact gggaccacgt gtgggtgctg tcccggtcca tggtgctgac cggcgaggcc
     2881 aagaccgccg tggagaacta cctgatcggc agccccgtgg tggacagcca gaaactggtg
     2941 tacagcgact tcagcgaggc cgcctgcaaa gtgaacaaca
//