LOCUS       AY822461                1774 bp    DNA     linear   SYN 23-APR-2005
DEFINITION  Yeast expression vector pJG515 chloramphenicol resistance protein
            (CmR) and CcdB (ccdB) genes, complete cds.
ACCESSION   AY822461
VERSION     AY822461.1  GI:62722841
KEYWORDS    .
SOURCE      Yeast expression vector pJG515
  ORGANISM  Yeast expression vector pJG515
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Geiser,J.R.
  TITLE     Recombinational cloning vectors for regulated expression in
            Saccharomyces cerevisiae
  JOURNAL   BioTechniques 38 (3), 378 (2005)
   PUBMED   15786805
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Geiser,J.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2004) Biological Sciences, Western Michigan
            University, 1903 West Michigan Avenue, Kalamazoo, MI 49008, USA
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Yeast expression vector pJG515"
                     /mol_type="other DNA"
                     /db_xref="taxon:308702"
     misc_feature    19..143
                     /note="attR1"
     gene            252..911
                     /gene="CmR"
     CDS             252..911
                     /gene="CmR"
                     /note="antibiotic resistance"
                     /codon_start=1
                     /product="chloramphenicol resistance protein"
                     /protein_id="AAX94725.1"
                     /db_xref="GI:62722842"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            1253..1558
                     /gene="ccdB"
     CDS             1253..1558
                     /gene="ccdB"
                     /note="for negative selection in E. coli with functional
                     DNa gyrase"
                     /codon_start=1
                     /product="CcdB"
                     /protein_id="AAX94726.1"
                     /db_xref="GI:62722843"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     misc_feature    1599..1723
                     /note="attR2"
ORIGIN      
        1 actagtggat cccccatcac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat
       61 ataaatatca atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa
      121 acacaacata tccagtcact atggcggccg cattaggcac cccaggcttt acactttatg
      181 cttccggctc gtataatgtg tggattttga gttaggatcc ggcgagattt tcaggagcta
      241 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
      301 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
      361 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
      421 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa
      481 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
      541 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
      601 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
      661 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg
      721 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
      781 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtctgtg
      841 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg
      901 gcggggcgta aacgcgtgga tccggcttac taaaagccag ataacagtat gcgtatttgc
      961 gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa
     1021 gaggtgtgct atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct
     1081 caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc
     1141 cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc
     1201 cggtttattg aaatgaacgg ctcttttgct gacgagaaca gggactggtg aaatgcagtt
     1261 taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga
     1321 tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc
     1381 agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat
     1441 gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct
     1501 cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat
     1561 gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg
     1621 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta
     1681 tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggtgatgggc tgcaggaatt
     1741 cgatatcaag cttatcgata ccgtcgacct cgag
//

