Team:Sheffield

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===SWITCHED ON !!!===
For the second time running [http://www.sheffield.ac.uk/ University of Sheffield] is proud to undertake another remarkable iGEM journey. The team constitute of three members specializing in different fields of engineering such as biomedical and system control. 












===Project Description===
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By modifying E.coli so that it can use a phytochrome- with a light receptor- from cyanobacteria as a trigger of protein generation. This pathway is controlled by a certain wavelength of red light, acting as a system switch for lacZ production. LacZ can react with substrate X-gal and form a blue precipitate as a reporter. However, other reporter genes can be attached to the lacZ gene, so different reporters can be expressed. From the fact that this mechanism is sensitive to a certain wavelength of light, we hope to create a system that can be sensitive to various wavelengths and hence triggering different protein generation. Through this the E.coli can become a wavelength sensor; a different wavelength can trigger a different production of protein, for example various types of fluorescent protein, giving a different a colour-scaled indication of the wavelength of the environment around the E.coli.

Our aim is to design an E.coli system that is sensitive to multiple wavelengths of light and therefore produce a colour indication of the specific wavelength it is exposed to. A pictorial description is shown below:


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==Sponsors==

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Special thanks to:

Prof Philip Wright, Prof Visakan Kadirkamanathan, Prof Alan Matthews, Dr Jagroop Pandhal, Dr Josselin Noirel, Tara 
Baldacchino and everyone in the bioincubator.





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