ULB/29 July 2009
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(New page: == July 29, 2009 == PCR (programme délétion) de RBS+Promoteur (avec et sans DMSO) en utilisant l’amorce forward du promoteur et l’amorce reverse du RBS<br/> PCR des gènes hfsH et hf...) |
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+ | |- | ||
+ | !align="right"|{{#calendar: title=ULB |year=2009 | month=08}} | ||
+ | |- | ||
+ | !align="right"|{{#calendar: title=ULB |year=2009 | month=09}} | ||
+ | |||
+ | |} | ||
+ | |||
+ | |||
+ | *PCR (deletion program) by RBS + promoter (with and without DMSO) using the forward primer of promoter and reverse primer of RBS. | ||
+ | |||
+ | *PCR of '''hFSH''' and '''hfsG''' with new primers. | ||
+ | |||
+ | *Gel migration of purified plasmid. | ||
+ | |||
+ | *Result: band of interest is thin. |
Latest revision as of 10:47, 21 October 2009
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- PCR (deletion program) by RBS + promoter (with and without DMSO) using the forward primer of promoter and reverse primer of RBS.
- PCR of hFSH and hfsG with new primers.
- Gel migration of purified plasmid.
- Result: band of interest is thin.