Team:Wash U/Protocol
From 2009.igem.org
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* Buffer AW2 | * Buffer AW2 | ||
* Buffer AE | * Buffer AE | ||
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'''Procedures''' | '''Procedures''' | ||
# Harvest cells by taking 1.5mL from overnight cultures and spinning them down to a pellet. | # Harvest cells by taking 1.5mL from overnight cultures and spinning them down to a pellet. | ||
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# Place the mini column in a clean collection tube and pipet 200 uL Buffer AE on the DNeasy membrane. Incubate at room temperature for 1 minute and then centrifuge for 1 minute at 12,000RPM. | # Place the mini column in a clean collection tube and pipet 200 uL Buffer AE on the DNeasy membrane. Incubate at room temperature for 1 minute and then centrifuge for 1 minute at 12,000RPM. | ||
# Repeat elution as described in step 9 with same collection tube to combine both elutes. | # Repeat elution as described in step 9 with same collection tube to combine both elutes. | ||
- | + | [[Team:Wash_U/Protocol#Procedures|Back To Top]] | |
Revision as of 19:12, 9 July 2009