Team:Chiba/Project

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[[Team:Chiba/Project/LuxR Mutants|-LuxR Mutants]]
[[Team:Chiba/Project/LuxR Mutants|-LuxR Mutants]]

Revision as of 08:12, 2 September 2009

Home

The Project

1, Parallel communication

-LuxR Mutants

2, Series communication

-Las Check

Modeling

Conclusions


Contents

Introduction

Project Design

Signaling System

In this project, we use acylated homoserine lactones (AHLs), signaling molecules used for [http://en.wikipedia.org/wiki/Quorum_sensing quorum sensing] in gram negative bacteria. Senders express LuxI or similar enzymes, which catalyze the production of AHLs, under the control of a constitutive (Tet) promoter. Each cell thus generates AHL more or less at a constant rate. AHL can freely permeate cell membranes and are detected by neighboring cells. Receivers constitutively express LuxR proteins (or a similar ortholog), the protein that detects AHL concentrations. When AHLs bind LuxR proteins, the AHL-LuxR complex activates the Lux promoter. The threshold [AHL] at which switching occurs is determined by the affinity of AHL for the particulr LuxR ortholog. (more about quorum sensing)


時限装置の集合体で時間の流れを表す

1, parallel communication

Team:Chiba/Project/Parallel

2, Series communication

Conclusions