Team:Warsaw/Calendar-Main/22 September 2009
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(New page: {{WarNotebook}} <!-- do not edit above me! --> <h3>Preparatio of biobricks to send</h3> <h4>Marcin</h4> Task 1: Preparation of bacterial cultures: '''Comment''': I prepare liquid bacte...) |
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* Reaction mixture composition: | * Reaction mixture composition: | ||
<pre> | <pre> | ||
- | + | 15 μl purified plasmid DNA product | |
1 μl enzyme 1 (Fermentas) | 1 μl enzyme 1 (Fermentas) | ||
1 μl enzyme 2 (Fermentas) | 1 μl enzyme 2 (Fermentas) | ||
- | + | 5 μl Buffer Tango (Fermentas) | |
- | + | 28 μl MQ water | |
</pre> | </pre> | ||
* Reactions were carried out 9 hour | * Reactions were carried out 9 hour |
Revision as of 13:12, 24 September 2009
Contents |
Preparatio of biobricks to send
Marcin
Task 1: Preparation of bacterial cultures:
Comment:
I prepare liquid bacterial cultures for isolation of followed biobricks:
- [http://partsregistry.org/Part:BBa_K177016BBa_K177016]
- [http://partsregistry.org/Part:BBa_K177035BBa_K177035]
- [http://partsregistry.org/Part:BBa_K177036BBa_K177036]
- [http://partsregistry.org/Part:BBa_K177042BBa_K177042]
- [http://partsregistry.org/Part:BBa_K177044BBa_K177044]
- [http://partsregistry.org/Part:BBa_K177046BBa_K177046]
Task 2: Isolation of plasmid from cultures described in Task 1
Methods:
- Plasmids were isolated using the A&A plasmid mini kit. Detailed procedure of the isolation is described [http://aabiot.com/products/dna_purification/plasmid_dna/plasmid_mini/protocol_plasmid_mini.pdf here]
Assembly of endosome detection operon
Marcin
Task 1: DNA digest to create following biobricks:
- [http://partsregistry.org/Part:BBa_K177037BBa_K177037]
- [http://partsregistry.org/Part:BBa_K177044BBa_K177044]
Methods:
- Constructs to digest:
- [http://partsregistry.org/Part:BBa_K177035BBa_K177035] - PstI, SpeI
- [http://partsregistry.org/Part:BBa_K177035BBa_K177035] - PstI, XbaI
- [http://partsregistry.org/Part:BBa_K177036BBa_K177036] - PstI, SpeI
- [http://partsregistry.org/Part:BBa_K177044BBa_K177044] - PstI, XbaI
- Reaction mixture composition:
15 μl purified plasmid DNA product 1 μl enzyme 1 (Fermentas) 1 μl enzyme 2 (Fermentas) 5 μl Buffer Tango (Fermentas) 28 μl MQ water
- Reactions were carried out 9 hour
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