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August/27 August 2009
From 2009.igem.org
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2. Ligation check was conducted on samples marked '''9-A, 9-B, 10-A, 10-B, 10-C, 10-D, 10-E''' from test plate culture. <br> | 2. Ligation check was conducted on samples marked '''9-A, 9-B, 10-A, 10-B, 10-C, 10-D, 10-E''' from test plate culture. <br> | ||
In the same electrophoresis run, vector-insert pairs for restriction enzyme-treated parts '''(16), (18) and (19)''' were also applied and separated, then cut out, purified and incubated with ligase.<br> | In the same electrophoresis run, vector-insert pairs for restriction enzyme-treated parts '''(16), (18) and (19)''' were also applied and separated, then cut out, purified and incubated with ligase.<br> | ||
| - | + | [[Image:Osaka090827.jpg]] | |
| + | UP : No.8 , 1-23L , No.10 , 1-6I , 2-13N , 1-2M , ladder | ||
| + | DOWN : No.9-A,B , No.10-A,B,C,D,E , vector , ladder | ||
<br> | <br> | ||
3. From ligation check, '''9-A, 9-B, 10-C''' were found to be defective.<br> | 3. From ligation check, '''9-A, 9-B, 10-C''' were found to be defective.<br> | ||
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5. 200ml of LB culture solution prepared.<br> | 5. 200ml of LB culture solution prepared.<br> | ||
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| + | [https://2009.igem.org/Team:Osaka/NOTES back to NOTES] | ||
Latest revision as of 08:16, 12 October 2009
1. Conducted colony check of transformed and incubated cultures (12) through (15) from 8/26.
No colonies were found on any of the plates.
2. Ligation check was conducted on samples marked 9-A, 9-B, 10-A, 10-B, 10-C, 10-D, 10-E from test plate culture.
In the same electrophoresis run, vector-insert pairs for restriction enzyme-treated parts (16), (18) and (19) were also applied and separated, then cut out, purified and incubated with ligase.
UP : No.8 , 1-23L , No.10 , 1-6I , 2-13N , 1-2M , ladder DOWN : No.9-A,B , No.10-A,B,C,D,E , vector , ladder
3. From ligation check, 9-A, 9-B, 10-C were found to be defective.
10-A, 10-B, 10-D, 10-E were picked up from the test growth plate and put into culture medium for overnight growth.
Miniprep of the above plasmids will be conducted tomorrow.
4. Transformation of ligated parts (20) and (21) followed by incubation to amplify the ligated plasmids.
5. 200ml of LB culture solution prepared.
