Team:Freiburg bioware/cloning

From 2009.igem.org

(Difference between revisions)
Line 273: Line 273:
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<p align="justify"><td align="left">
-
MKKIWLALAGLVLAFSASATGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAA
+
MAGMKKIWLALAGLVLAFSASATGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAA
YPDHITKYGKCGWAEYTPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIG
YPDHITKYGKCGWAEYTPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIG
GVTQVGVSNVLSTDNKNYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIG
GVTQVGVSNVLSTDNKNYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIG
Line 305: Line 305:
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
+
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT
ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT
Line 339: Line 339:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
GWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP
+
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP
EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY
EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY
IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
Line 371: Line 371:
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
+
ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
Line 405: Line 405:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
HHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
+
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
Line 438: Line 438:
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
+
ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
Line 473: Line 473:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
HHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
+
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
Line 507: Line 507:
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
+
ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
Line 543: Line 543:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
HHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
+
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
Line 578: Line 578:
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
+
ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
Line 615: Line 615:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
HHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
+
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
Line 650: Line 650:
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
+
ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
Line 683: Line 683:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
HHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
+
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
Line 717: Line 717:
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
+
ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
Line 751: Line 751:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
HHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
+
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
Line 787: Line 787:
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
+
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT
ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT
Line 822: Line 822:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
GWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP
+
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP
EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY
EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY
IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
Line 859: Line 859:
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
+
ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
Line 894: Line 894:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
HHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
+
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
Line 932: Line 932:
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
+
ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
Line 965: Line 965:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
HHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
+
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
Line 997: Line 997:
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
+
ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
Line 1,031: Line 1,031:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
HHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
+
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
Line 1,065: Line 1,065:
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
+
ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
Line 1,100: Line 1,100:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
HHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
+
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
Line 1,136: Line 1,136:
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGG
+
ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG
CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC
ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC
Line 1,173: Line 1,173:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
HHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP
+
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP
EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY
EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY
IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
Line 1,210: Line 1,210:
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
+
ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
Line 1,246: Line 1,246:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
HHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
+
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
Line 1,284: Line 1,284:
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
+
ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
Line 1,321: Line 1,321:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
HHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
+
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
Line 1,360: Line 1,360:
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
GGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTG
+
ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTG
CCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCA
CCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCA
AGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAG
AGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAG
Line 1,393: Line 1,393:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
HHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEG
+
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEG
KSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYII
KSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYII
GYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACK
GYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACK
Line 1,428: Line 1,428:
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
+
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC
ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC
Line 1,463: Line 1,463:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
GWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP
+
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP
EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY
EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY
IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
Line 1,497: Line 1,497:
nucleotid sequence of ORF-1:<br>
nucleotid sequence of ORF-1:<br>
-
GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
+
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC
ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC
Line 1,533: Line 1,533:
<table border="0">
<table border="0">
<p align="justify"><td align="left">
<p align="justify"><td align="left">
-
GWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP
+
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP
EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY
EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY
IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
Line 1,551: Line 1,551:
<br>
<br>
<br>
<br>
 +
 +
 +
 +
 +
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+Dig+middellinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
 +
</div>
 +
<div class="art-PostContent">
 +
<div style="text-align: center;">
 +
</div><a name="4"></a>
 +
 +
<table border="5">
 +
<tr>
 +
<td align="center">
 +
<img src="https://static.igem.org/mediawiki/2009/thumb/9/94/Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokA_pMA.jpg" name="Strep+Dig+middellinker+FokA in pMA Vector" width="480" height="480" />
 +
<br><p align="center">Strep+Dig+middellinker+FokA in pMA Vector</p>
 +
</td>
 +
<td align="left">
 +
nucleotid sequence of ORF-1:<br>
 +
 +
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 +
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 +
AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC
 +
AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 +
GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG
 +
GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 +
TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC
 +
CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA
 +
AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 +
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 +
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA
 +
AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT
 +
GGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAA
 +
ATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAAC
 +
TGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAA
 +
GTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGG
 +
TGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCG
 +
ATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTG
 +
CCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGAC
 +
TCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAA
 +
GCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAAC
 +
TATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGC
 +
CGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCG
 +
GGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATC
 +
AATTTTACCGGTTAA
 +
 +
 +
 +
 +
 +
</td>
 +
</tr>
 +
</table>
 +
<br>
 +
<h4>AA sequence of ORF-1:<br></h4>
 +
<table border="0">
 +
<p align="justify"><td align="left">
 +
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY
 +
TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK
 +
NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 +
AACKVNNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARN
 +
STQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNL
 +
PIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNH
 +
KTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 +
 +
 +
 +
 +
 +
<br><br></p></td></table>
 +
 +
<h4>Commented GenBank file</h4>
 +
<li> <a href="https://static.igem.org/mediawiki/2009/9/9f/Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokA_pMA.txt">Strep+Dig+middellinker+FokA_pMA.txt</a></li>
 +
<br>
 +
<br>
 +
<br>
 +
 +
 +

Revision as of 18:54, 13 October 2009

FREiGEM

overview


His+FluA+Split+Foki_pMA
His+Dig+Split+FokA_pMA
DsbA+His+Dig+Split+FokA_pMA

 Parts

 His+FluA+Split+Foki_pMA


His+FluA+SplitLinker+Foki in pMA Vector

 nucleotid sequence of ORF-1:
ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA ACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAA GTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCT GCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGA TCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAG TTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCG TAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCG TTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGT CAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAA ACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCG AGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCC CAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAG TGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGA CTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACC GGTTAA

AA sequence of ORF-1:

MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP
EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY
IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
CKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQ
DRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIG
QADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITN
SNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

Commented GenBank file

  • His+FluA+Split+Foki_pMA.txt



    •  His+Dig+Split+FokA_pMA


    His+Dig+Split+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACCAGCC TGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCGGCAA ATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATG TGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAA GACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGG CTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTT ATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCC TATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCG TTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAAT GGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTG AGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCA TAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTG GCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGT AAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIE LIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKA YSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKA QLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+Split+FokA_pMA.txt



    •  DsbA+His+Dig+Split+FokA_pMA


    DsbA+His+Dig+Split+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGC ATCGGCGACCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACG ACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAG TACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAA GTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGA AGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGC ACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTA CACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCG ACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAG AAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGAC CGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGG TGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAA GTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACC AGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCG GCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAA TATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTAC CCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCT ATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCC ATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAA AGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGC AGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAAC GAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTT CGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGA ACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTG ATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCG CCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGMKKIWLALAGLVLAFSASATGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAA YPDHITKYGKCGWAEYTPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIG GVTQVGVSNVLSTDNKNYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIG SPVVDSQKLVYSDFSEAACKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEE KKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGA IYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSV TEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKF NNGEINFTG

    Commented GenBank file

  • DsbA+His+Dig+Split+FokA_pMA.txt



    •  Strep+FluA+ShortLinker+FokI_pMA


    Strep+FluA+ShortLinker+FokI in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA ACAACACCGGCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAA AAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGA ACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGA AAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTG GGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGAT CGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATC TGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAG ACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTC AAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCA ACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGG GCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGC AGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGA TCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFM KVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEENQ TRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELLI GGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+FluA+ShortLinker+FokI_pMA.txt



    •  His+FluA+middellinker+FokA_pMA


    His+FluA+middellinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAG AAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATAT CGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAA TGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACAT CTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCC GATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATA ACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAAC CAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCC GTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAG GCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAAT GGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAA AGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGG AGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFF MKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKEN QTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELL IGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+middellinker+FokA_pMA.txt



    •  His+FluA+middellinker+Foki_pMA


    His+FluA+middellinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAG AAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATAT CGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAA TGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACAT CTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCC GATCGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATA ATCTGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAAT CAGACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCC GTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAG GCAACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAAT GGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAA AGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCG AGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFF MKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEEN QTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELL IGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+middellinker+Foki_pMA.txt



    •  His+FluA+shortlinker+FokA_pMA


    His+FluA+shortlinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAG CTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGA GATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGG AGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGC CGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGG CGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTG GTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAAC AAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTAC AGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAG CACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTG TCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCT GACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTA CCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVY GYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRN KHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGE MIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+shortlinker+FokA_pMA.txt



    •  His+FluA+shortlinker+Foki_pMA


    His+FluA+shortlinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAG CTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGA GATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGG AGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGC CGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGG CGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTG GTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAAC AAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCAC CGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAG CCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTG AGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCT GACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTA CCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVY GYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEENQTRN KHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELLIGGE MIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+shortlinker+Foki_pMA.txt



    •  His+Dig+longlinker+FokA_pMA


    His+Dig+longlinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT TCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATC CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG TAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATT ATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCA ATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCG TAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCG TTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTAT AAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGT TCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGA CCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAAT TTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLP IGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHK TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+longlinker+FokA_pMA.txt



    •  His+Dig+longlinker+Foki_pMA


    His+Dig+longlinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT TCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATC CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG TAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATT ATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCG ATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCG TAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTG TCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTAT AAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGT GCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCA CCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAAT TTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLP IGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHI TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+longlinker+Foki_pMA.txt



    •  Strep+FluA+Split+Foki_pMA


    Strep+FluA+Split+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA ACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAA GTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCT GCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGA TCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAG TTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCG TAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCG TTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGT CAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAA ACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCG AGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCC CAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAG TGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGA CTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACC GGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQ DRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIG QADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITN SNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+FluA+Split+Foki_pMA.txt



    •  His+Dig+middellinker+FokA_pMA


    His+Dig+middellinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT TCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCA CAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCC GTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTC ATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACC TGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCG TTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCT GATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACAT CAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCA AATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTG ACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGA AGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGG AAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDR ILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQA DEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSN GAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+middellinker+FokA_pMA.txt



    •  His+Dig+middellinker+Foki_pMA


    His+Dig+middellinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT TCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCA CAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCC GTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTC ATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACC AGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCG TGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCT GATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCT GAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCA AATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTG ACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGA GGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGG AAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDR ILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQA DEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSN GAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+middellinker+Foki_pMA.txt



    •  His+Dig+shortlinker+FokA_pMA


    His+Dig+shortlinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTACCGGC AAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATA TGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCC AAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTAT GGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCAT TTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAG CCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAG CGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGA ATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCG TGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAAC CATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGAT TGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCC GTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEM KVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQ RYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVL SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+shortlinker+FokA_pMA.txt



    •  His+Dig+shortlinker+Foki_pMA


    His+Dig+shortlinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTACCGGC AAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATA TGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCC AAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTAT GGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCAT TTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAG CGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAA CGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGA ATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCG TGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAAC CATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGAT CGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCC GTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEM KVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEME RYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVL SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+shortlinker+Foki_pMA.txt



    •  His+Dig+Split+Foki_pMA


    His+Dig+Split+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACCAGCC TGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCGGCAA ATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATG TGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAA GACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGG CTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTT ATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAGCG TATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAACG TTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGAAT GGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTG AGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAACCA TATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCG GTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGT AAATTCAACAATGGCGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIE LIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKA YSGGYNLPIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKA QLTRLNHITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+Dig+Split+Foki_pMA.txt



    •  His+FluA+longlinker+FokA_pMA


    His+FluA+longlinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCT GAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCC TCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACC GTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTAT CGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATAC CGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCTATA GCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTAT GTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAATGGTG GAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCG GCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAA ACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGG TGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAAT TCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRY VKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSV EELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+longlinker+FokA_pMA.txt



    •  His+FluA+longlinker+Foki_pMA


    His+FluA+longlinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG GCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCT GAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCC TCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACC GTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTAT CGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTATAC CGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAGCGTATT CTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAACGTTAT GTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGAATGGTG GAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCG GCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAACCATATC ACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGG AGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAAT TCAACAATGGCGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV NNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV MEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERY VEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSV EELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+longlinker+Foki_pMA.txt



    •  His+FluA+Split+FokA_pMA


    His+FluA+Split+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTG CCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCA AGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAG TGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAG ATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACC CCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGC GGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAA CTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACT GGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCC AAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCA GAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACA CCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATG AATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCA CAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCC GTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTC ATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACC TGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCG TTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCT GATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACAT CAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCA AATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTG ACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGA AGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGG AAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEG KSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYII GYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACK VNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDR ILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQA DEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSN GAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • His+FluA+Split+FokA_pMA.txt



    •  Strep+Dig+longlinker+FokA_pMA


    Strep+Dig+longlinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCT GGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGA GAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATA TCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAA ATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACA TCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCC CGATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTAT AACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAA CCAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATC CGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAA GGCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAA TGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCA AAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGG GAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIA RNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGY NLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRL NHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+Dig+longlinker+FokA_pMA.txt



    •  Strep+Dig+longlinker+Foki_pMA


    Strep+Dig+longlinker+Foki in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCT GGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGA GAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATA TCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAA ATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACA TCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCC CGATCGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTAT AATCTGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAA TCAGACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATC CGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAA GGCAACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAA TGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCA AAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGC GAGATCAATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA CKVNNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIA RNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGY NLPIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRL NHITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+Dig+longlinker+Foki_pMA.txt



    •  Strep+Dig+middellinker+FokA_pMA


    Strep+Dig+middellinker+FokA in pMA Vector

    		 nucleotid sequence of ORF-1:
    ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT GGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAA ATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAAC TGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAA GTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGG TGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCG ATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTG CCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGAC TCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAA GCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAAC TATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGC CGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCG GGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATC AATTTTACCGGTTAA

    AA sequence of ORF-1:

    MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARN STQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNL PIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNH KTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG

    Commented GenBank file

  • Strep+Dig+middellinker+FokA_pMA.txt





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    Retrieved from "http://2009.igem.org/Team:Freiburg_bioware/cloning"