Team:Freiburg bioware/cloning1

From 2009.igem.org

(Difference between revisions)
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<a name="2"></a>
 
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<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+FluA+Split+Foki_pMA<span class="art-PostHeader"></span> </h3>
 
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<div class="art-PostContent">
 
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<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
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<table border="5">
 
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<tr>
 
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<td align="center">
 
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<img src="https://static.igem.org/mediawiki/2009/3/37/Freiburg09_His%2BFluA%2BSplit%2BFokI_pMA.jpg" name="His+FluA+SplitLinker+Foki in pMA Vector" width="480" height="480" />
 
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<br><p align="center">His+FluA+SplitLinker+Foki in pMA Vector</p>
 
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</td>
 
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<td align="left">
 
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nucleotid sequence of ORF-1:<br>
 
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ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG
 
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CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
 
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ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT
 
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GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
 
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GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG
 
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CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC
 
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GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA
 
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CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG
 
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GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
 
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GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
 
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CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA
 
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ACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAA
 
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GTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCT
 
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GCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGA
 
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TCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAG
 
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TTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCG
 
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TAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCG
 
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TTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGT
 
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CAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAA
 
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ACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCG
 
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AGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCC
 
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CAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAG
 
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TGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGA
 
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CTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACC
 
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GGTTAA
 
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</td>
 
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</tr>
 
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</table>
 
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<br>
 
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<h4>AA sequence of ORF-1:<br></h4>
 
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<table border="0">
 
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<p align="justify"><td align="left">
 
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MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP<br>
 
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EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY<br>
 
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IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA<br>
 
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CKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQ<br>
 
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DRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIG<br>
 
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QADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITN<br>
 
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SNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG<br><br></p></td></table>
 
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<h4>Commented GenBank file</h4>
 
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<li> <a href="https://static.igem.org/mediawiki/2009/d/d8/Freiburg09_His%2BFluA%2BSplit%2BFokI_pMA.txt">His+FluA+Split+Foki_pMA.txt</a></li>
 
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<br>
 
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<br>
 
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<br>
 
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</div><a name="3"></a>
 
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<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+Dig+Split+FokA_pMA<span class="art-PostHeader"></span> </h3>
 
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<div class="art-PostContent">
 
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<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
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-
<table border="5">
 
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<tr>
 
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<td align="center">
 
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<img src="https://static.igem.org/mediawiki/2009/3/34/Freiburg09_His%2BDig%2BSplit%2BFokA_pMA.JPG" name="His+Dig+Split+FokA in pMA Vector" width="480" height="480" />
 
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<br><p align="center">His+Dig+Split+FokA in pMA Vector</p>
 
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</td>
 
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<td align="left">
 
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nucleotid sequence of ORF-1:<br>
 
-
 
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ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG
 
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CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
 
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ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC
 
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GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
 
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GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG
 
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CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC
 
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ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA
 
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CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG
 
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GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
 
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GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
 
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CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA
 
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ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACCAGCC
 
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TGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCGGCAA
 
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ATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATG
 
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TGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAA
 
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GACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGG
 
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CTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTT
 
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ATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCC
 
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TATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCG
 
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TTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAAT
 
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GGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTG
 
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AGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCA
 
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TAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTG
 
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GCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGT
 
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AAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
 
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</td>
 
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</tr>
 
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</table>
 
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<br>
 
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<h4>AA sequence of ORF-1:<br></h4>
 
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<table border="0">
 
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<p align="justify"><td align="left">
 
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MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP
 
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EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY
 
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IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
 
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CKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIE
 
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LIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKA
 
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YSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKA
 
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QLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
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<br><br></p></td></table>
 
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<h4>Commented GenBank file</h4>
 
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<li> <a href="https://static.igem.org/mediawiki/2009/d/dd/Freiburg09_His%2BDig%2BSplit%2BFokA_pMA.txt">His+Dig+Split+FokA_pMA.txt</a></li>
 
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<br>
 
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<br>
 
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<br>
 
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</div><a name="4"></a>
 
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<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;DsbA+His+Dig+Split+FokA_pMA<span class="art-PostHeader"></span> </h3>
 
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</div>
 
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<div class="art-PostContent">
 
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<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
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<img src="https://static.igem.org/mediawiki/2009/4/4f/Freiburg09_DsbA%2BHis%2BDig%2BSplit%2BFokA_pMA.JPG" name="DsbA+His+Dig+Split+FokA in pMA Vector" width="480" height="480" />
 
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<br><p align="center">DsbA+His+Dig+Split+FokA in pMA Vector</p>
 
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</td>
 
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<td align="left">
 
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nucleotid sequence of ORF-1:<br>
 
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ATGAAAAAGATTTGGCTGGCGCTGGCTGGTTTAGTTTTAGCGTTTAGCGC
 
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ATCGGCGACCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACG
 
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ACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAG
 
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TACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAA
 
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GTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGA
 
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AGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGC
 
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ACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTA
 
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CACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCG
 
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ACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAG
 
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AAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGAC
 
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CGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGG
 
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TGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAA
 
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GTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACC
 
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AGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCG
 
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GCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAA
 
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TATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTAC
 
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CCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCT
 
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ATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCC
 
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ATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAA
 
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AGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGC
 
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AGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAAC
 
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GAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTT
 
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CGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGA
 
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ACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTG
 
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ATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCG
 
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CCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
 
-
 
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</td>
 
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</tr>
 
-
</table>
 
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<br>
 
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<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
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MAGMKKIWLALAGLVLAFSASATGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAA
 
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YPDHITKYGKCGWAEYTPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIG
 
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GVTQVGVSNVLSTDNKNYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIG
 
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SPVVDSQKLVYSDFSEAACKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEE
 
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KKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGA
 
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IYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSV
 
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TEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKF
 
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NNGEINFTG
 
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<br><br></p></td></table>
 
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<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/4/4d/Freiburg09_DsbA%2BHis%2BDig%2BSplit%2BFokA_pMA.txt">DsbA+His+Dig+Split+FokA_pMA.txt</a></li>
 
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<br>
 
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<br>
 
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<br>
 
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</div><a name="5"></a>
 
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<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+ShortLinker+FokI_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/0/03/Freiburg09_Strep%2BFluA%2BShortLinker%2BFokI_pMA.jpg" name="Strep+FluA+ShortLinker+FokI in pMA Vector" width="480" height="480" />
 
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<br><p align="center">Strep+FluA+ShortLinker+FokI in pMA Vector</p>
 
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</td>
 
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<td align="left">
 
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nucleotid sequence of ORF-1:<br>
 
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ATGGCC
 
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GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
 
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CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
 
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ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT
 
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GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
 
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GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG
 
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CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC
 
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GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA
 
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CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG
 
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GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
 
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GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
 
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CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA
 
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ACAACACCGGCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAA
 
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AAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGA
 
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ACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGA
 
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AAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTG
 
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GGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGAT
 
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CGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATC
 
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TGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAG
 
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ACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTC
 
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AAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCA
 
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ACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGG
 
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GCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGC
 
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AGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGA
 
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TCAATTTTACCGGTTAA
 
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-
 
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</td>
 
-
</tr>
 
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</table>
 
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<br>
 
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<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
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MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP
 
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EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY
 
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IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
 
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CKVNNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFM
 
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KVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEENQ
 
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TRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELLI
 
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GGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
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<br><br></p></td></table>
 
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<h4>Commented GenBank file</h4>
 
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<li> <a href="https://static.igem.org/mediawiki/2009/7/7e/Freiburg09_Strep%2BFluA%2BShortLinker%2BFokI_pMA.txt">Strep+FluA+ShortLinker+FokI_pMA.txt</a></li>
 
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<br>
 
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<br>
 
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<br>
 
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</div><a name="6"></a>
 
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<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+FluA+middellinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/a/ad/Freiburg09_FluA%2BHis%2Bmiddellinker%2BFokA_pMA.jpg/600px-Freiburg09_FluA%2BHis%2Bmiddellinker%2BFokA_pMA.jpg" name="His+FluA+middellinker+FokA in pMA Vector" width="480" height="480" />
 
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<br><p align="center">His+FluA+middellinker+FokA in pMA Vector</p>
 
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</td>
 
-
<td align="left">
 
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nucleotid sequence of ORF-1:<br>
 
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ATGGCCGGC
 
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CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
 
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CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
 
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GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
 
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GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA
 
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CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG
 
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TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC
 
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TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA
 
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CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG
 
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ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
 
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ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
 
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ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG
 
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GCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAG
 
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AAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATAT
 
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CGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAA
 
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TGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACAT
 
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CTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCC
 
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GATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATA
 
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ACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAAC
 
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CAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCC
 
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GTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAG
 
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GCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAAT
 
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GGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAA
 
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AGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGG
 
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AGATCAATTTTACCGGTTAA
 
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</td>
 
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</tr>
 
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</table>
 
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<br>
 
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<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
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MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
 
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SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
 
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YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
 
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NNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFF
 
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MKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKEN
 
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QTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELL
 
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IGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
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<br><br></p></td></table>
 
-
 
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<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/7/78/Freiburg09_FluA%2BHis%2Bmiddellinker%2BFokA_pMA.txt">His+FluA+middellinker+FokA_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
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<br>
 
-
 
-
</div><a name="7"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+FluA+middellinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/c/ce/Freiburg09_FluA%2BHis%2Bmiddellinker%2BFoki_pMA.jpg/600px-Freiburg09_FluA%2BHis%2Bmiddellinker%2BFoki_pMA.jpg" name="His+FluA+middellinker+Foki in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">His+FluA+middellinker+Foki in pMA Vector</p><br>
 
-
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243014">link to part registry</a>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGGCCGGC
 
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
 
-
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
 
-
GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
 
-
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA
 
-
CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG
 
-
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC
 
-
TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA
 
-
CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG
 
-
ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
 
-
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
 
-
ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG
 
-
GCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAG
 
-
AAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATAT
 
-
CGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAA
 
-
TGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACAT
 
-
CTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCC
 
-
GATCGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATA
 
-
ATCTGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAAT
 
-
CAGACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCC
 
-
GTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAG
 
-
GCAACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAAT
 
-
GGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAA
 
-
AGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCG
 
-
AGATCAATTTTACCGGTTAA
 
-
 
-
 
-
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
 
-
SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
 
-
YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
 
-
NNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFF
 
-
MKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEEN
 
-
QTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELL
 
-
IGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/9/92/Freiburg09_FluA%2BHis%2Bmiddellinker%2BFoki_pMA.txt">His+FluA+middellinker+Foki_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
 
-
</div><a name="8"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+FluA+shortlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/d/d4/Freiburg09_FluA%2BHis%2Bshortlinker%2BFokA_pMA.jpg/600px-Freiburg09_FluA%2BHis%2Bshortlinker%2BFokA_pMA.jpg" name="His+FluA+shortlinker+FokA in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">His+FluA+shortlinker+FokA in pMA Vector</p>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGGCCGGC
 
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
 
-
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
 
-
GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
 
-
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA
 
-
CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG
 
-
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC
 
-
TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA
 
-
CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG
 
-
ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
 
-
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
 
-
ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG
 
-
GCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAG
 
-
CTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGA
 
-
GATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGG
 
-
AGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGC
 
-
CGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGG
 
-
CGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTG
 
-
GTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAAC
 
-
AAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTAC
 
-
AGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAG
 
-
CACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTG
 
-
TCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCT
 
-
GACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTA
 
-
CCGGTTAA
 
-
 
-
 
-
 
-
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
 
-
SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
 
-
YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
 
-
NNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVY
 
-
GYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKENQTRN
 
-
KHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEELLIGGE
 
-
MIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/4/46/Freiburg09_FluA%2BHis%2Bshortlinker%2BFokA_pMA.txt">His+FluA+shortlinker+FokA_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
 
-
</div><a name="9"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+FluA+shortlinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/b/bc/Freiburg09_FluA%2BHis%2Bshortlinker%2BFokI_pMA.jpg/600px-Freiburg09_FluA%2BHis%2Bshortlinker%2BFokI_pMA.jpg" name="His+FluA+shortlinker+Foki in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">His+FluA+shortlinker+Foki in pMA Vector</p><br>
 
-
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243013">link to part registry</a>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGGCCGGC
 
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
 
-
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
 
-
GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
 
-
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA
 
-
CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG
 
-
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC
 
-
TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA
 
-
CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG
 
-
ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
 
-
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
 
-
ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG
 
-
GCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAG
 
-
CTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGA
 
-
GATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGG
 
-
AGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGC
 
-
CGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGG
 
-
CGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTG
 
-
GTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAAC
 
-
AAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCAC
 
-
CGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAG
 
-
CCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTG
 
-
AGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCT
 
-
GACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTA
 
-
CCGGTTAA
 
-
 
-
 
-
 
-
 
-
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
 
-
SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
 
-
YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
 
-
NNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEFFMKVY
 
-
GYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERYVEENQTRN
 
-
KHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSVEELLIGGE
 
-
MIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/7/7d/Freiburg09_FluA%2BHis%2Bshortlinker%2BFokI_pMA.txt">His+FluA+shortlinker+Foki_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
 
-
</div><a name="10"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+Dig+longlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/7/7d/Freiburg09_His%2BDig%2Blonglinker%2BFokA_pMA.jpg/600px-Freiburg09_His%2BDig%2Blonglinker%2BFokA_pMA.jpg" name="His+Dig+longlinker+FokA in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">His+Dig+longlinker+FokA in pMA Vector</p>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGGCCGGC
 
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
 
-
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
 
-
GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
 
-
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA
 
-
CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG
 
-
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC
 
-
GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA
 
-
CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG
 
-
ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
 
-
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
 
-
ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA
 
-
ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT
 
-
TCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATC
 
-
CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA
 
-
TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG
 
-
ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG
 
-
TAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATT
 
-
ATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCA
 
-
ATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCG
 
-
TAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCG
 
-
TTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTAT
 
-
AAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGT
 
-
TCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGA
 
-
CCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAAT
 
-
TTTACCGGTTAA
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
 
-
SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
 
-
YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
 
-
NNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS
 
-
TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLP
 
-
IGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHK
 
-
TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/0/07/Freiburg09_His%2BDig%2Blonglinker%2BFokA_pMA.txt">His+Dig+longlinker+FokA_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
 
-
</div><a name="11"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+Dig+longlinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/6/64/Freiburg09_His%2BDig%2Blonglinker%2BFoki_pMA.JPG/600px-Freiburg09_His%2BDig%2Blonglinker%2BFoki_pMA.JPG" name="His+Dig+longlinker+Foki in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">His+Dig+longlinker+Foki in pMA Vector</p>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGGCCGGC
 
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
 
-
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
 
-
GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
 
-
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA
 
-
CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG
 
-
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC
 
-
GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA
 
-
CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG
 
-
ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
 
-
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
 
-
ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA
 
-
ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT
 
-
TCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATC
 
-
CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA
 
-
TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG
 
-
ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG
 
-
TAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATT
 
-
ATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCG
 
-
ATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCG
 
-
TAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTG
 
-
TCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTAT
 
-
AAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGT
 
-
GCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCA
 
-
CCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAAT
 
-
TTTACCGGTTAA
 
-
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
 
-
SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
 
-
YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
 
-
NNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS
 
-
TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLP
 
-
IGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHI
 
-
TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/e/ef/Freiburg09_His%2BDig%2Blonglinker%2BFoki_pMA.txt">His+Dig+longlinker+Foki_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
 
-
 
-
</div><a name="12"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+Split+Foki_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/f/fa/Freiburg09_Strep%2BFluA%2BSplit%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2BSplit%2BFokI_pMA.jpg" name="Strep+FluA+Split+Foki in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">Strep+FluA+Split+Foki in pMA Vector</p>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGGCC
 
-
GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
 
-
CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
 
-
ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT
 
-
GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
 
-
GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG
 
-
CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC
 
-
GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA
 
-
CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG
 
-
GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
 
-
GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
 
-
CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA
 
-
ACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAA
 
-
GTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCT
 
-
GCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGA
 
-
TCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAG
 
-
TTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCG
 
-
TAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCG
 
-
TTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGT
 
-
CAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAA
 
-
ACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCG
 
-
AGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCC
 
-
CAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAG
 
-
TGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGA
 
-
CTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACC
 
-
GGTTAA
 
-
 
-
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP
 
-
EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY
 
-
IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
 
-
CKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQ
 
-
DRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIG
 
-
QADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITN
 
-
SNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
 
-
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/8/8d/Freiburg09_Strep%2BFluA%2BSplit%2BFokI_pMA.txt">Strep+FluA+Split+Foki_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
 
-
 
-
</div><a name="13"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+Dig+middellinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/c/c7/Freiburg09_His%2BDig%2Bmiddellinker%2BFokA_pMA.jpg/600px-Freiburg09_His%2BDig%2Bmiddellinker%2BFokA_pMA.jpg" name="His+Dig+middellinker+FokA in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">His+Dig+middellinker+FokA in pMA Vector</p>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGGCCGGC
 
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
 
-
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
 
-
GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
 
-
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA
 
-
CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG
 
-
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC
 
-
GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA
 
-
CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG
 
-
ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
 
-
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
 
-
ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA
 
-
ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT
 
-
TCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCA
 
-
CAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCC
 
-
GTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTC
 
-
ATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACC
 
-
TGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCG
 
-
TTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCT
 
-
GATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACAT
 
-
CAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCA
 
-
AATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTG
 
-
ACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGA
 
-
AGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGG
 
-
AAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
 
-
 
-
 
-
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
 
-
SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
 
-
YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
 
-
NNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDR
 
-
ILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQA
 
-
DEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSN
 
-
GAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
 
-
 
-
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/8/8a/Freiburg09_His%2BDig%2Bmiddellinker%2BFokA_pMA.txt">His+Dig+middellinker+FokA_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
 
-
 
-
</div><a name="14"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+Dig+middellinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/3/3b/Freiburg09_His%2BDig%2Bmiddellinker%2BFokI_pMA.jpg/600px-Freiburg09_His%2BDig%2Bmiddellinker%2BFokI_pMA.jpg" name="His+Dig+middellinker+Foki in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">His+Dig+middellinker+Foki in pMA Vector</p>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGGCCGGC
 
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
 
-
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
 
-
GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
 
-
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA
 
-
CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG
 
-
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC
 
-
GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA
 
-
CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG
 
-
ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
 
-
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
 
-
ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA
 
-
ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTGGTGGT
 
-
TCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCA
 
-
CAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCC
 
-
GTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTC
 
-
ATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACC
 
-
AGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCG
 
-
TGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCT
 
-
GATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCT
 
-
GAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCA
 
-
AATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTG
 
-
ACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGA
 
-
GGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGG
 
-
AAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA
 
-
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
 
-
SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
 
-
YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
 
-
NNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDR
 
-
ILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQA
 
-
DEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSN
 
-
GAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/9/9f/Freiburg09_His%2BDig%2Bmiddellinker%2BFokI_pMA.txt">His+Dig+middellinker+Foki_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
</div><a name="15"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+Dig+shortlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/5/58/Freiburg09_His%2BDig%2Bshortlinker%2BFokA_pMA.jpg/600px-Freiburg09_His%2BDig%2Bshortlinker%2BFokA_pMA.jpg" name="His+Dig+shortlinker+FokA in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">His+Dig+shortlinker+FokA in pMA Vector</p>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGGCCGGC
 
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
 
-
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
 
-
GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
 
-
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA
 
-
CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG
 
-
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC
 
-
GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA
 
-
CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG
 
-
ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
 
-
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
 
-
ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA
 
-
ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTACCGGC
 
-
AAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATA
 
-
TGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCC
 
-
AAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTAT
 
-
GGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCAT
 
-
TTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAG
 
-
CCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAG
 
-
CGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGA
 
-
ATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCG
 
-
TGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAAC
 
-
CATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGAT
 
-
TGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCC
 
-
GTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
 
-
 
-
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
 
-
SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
 
-
YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
 
-
NNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEM
 
-
KVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQ
 
-
RYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVL
 
-
SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/d/d2/Freiburg09_His%2BDig%2Bshortlinker%2BFokA_pMA.txt">His+Dig+shortlinker+FokA_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
 
-
</div><a name="16"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+Dig+shortlinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/1/15/Freiburg09_His%2BDig%2Bshortlinker%2BFoki_pMA.jpg/600px-Freiburg09_His%2BDig%2Bshortlinker%2BFoki_pMA.jpg" name="His+Dig+shortlinker+Foki in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">His+Dig+shortlinker+Foki in pMA Vector</p>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGGCCGGC
 
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
 
-
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
 
-
GGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTACGGCAAGTGC
 
-
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCCGGTA
 
-
CAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCGCCTACCCTG
 
-
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACCATCGGCGGC
 
-
GTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAACAAGAACTA
 
-
CATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGGGCCACTGGG
 
-
ACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
 
-
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
 
-
ACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGAACAACAGCA
 
-
ACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCTGGTACCGGC
 
-
AAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATA
 
-
TGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCC
 
-
AAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTAT
 
-
GGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCAT
 
-
TTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAG
 
-
CGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAA
 
-
CGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGA
 
-
ATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCG
 
-
TGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAAC
 
-
CATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGAT
 
-
CGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCC
 
-
GTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA
 
-
 
-
 
-
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTPEGK
 
-
SVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNYIIG
 
-
YFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
 
-
NNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEM
 
-
KVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEME
 
-
RYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVL
 
-
SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/b/b5/Freiburg09_His%2BDig%2Bshortlinker%2BFoki_pMA.txt">His+Dig+shortlinker+Foki_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
 
-
 
-
</div><a name="17"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+Dig+Split+Foki_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/d/d7/Freiburg09_His%2BDig%2BSplit%2BFokI_pMA.jpg/600px-Freiburg09_His%2BDig%2BSplit%2BFokI_pMA.jpg" name="His+Dig+Split+Foki in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">His+Dig+Split+Foki in pMA Vector</p><br>
 
-
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243018">link to part registry</a>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGGCCGGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGG
 
-
CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
 
-
ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC
 
-
GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
 
-
GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG
 
-
CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC
 
-
ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA
 
-
CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG
 
-
GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
 
-
GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
 
-
CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA
 
-
ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGACCAGCC
 
-
TGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACACCGGCAA
 
-
ATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATG
 
-
TGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAA
 
-
GACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGG
 
-
CTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTT
 
-
ATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAGCG
 
-
TATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAACG
 
-
TTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGAAT
 
-
GGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTG
 
-
AGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAACCA
 
-
TATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCG
 
-
GTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGT
 
-
AAATTCAACAATGGCGAGATCAATTTTACCGGTTAA
 
-
 
-
 
-
 
-
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP
 
-
EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY
 
-
IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
 
-
CKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIE
 
-
LIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKA
 
-
YSGGYNLPIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKA
 
-
QLTRLNHITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
 
-
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/1/11/Freiburg09_His%2BDig%2BSplit%2BFokI_pMA.txt">His+Dig+Split+Foki_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
 
-
 
-
</div><a name="18"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+FluA+longlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/9/9f/Freiburg09_His%2BFluA%2Blonglinker%2BFokA_pMA.jpg/600px-Freiburg09_His%2BFluA%2Blonglinker%2BFokA_pMA.jpg" name="His+FluA+longlinker+FokA in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">His+FluA+longlinker+FokA in pMA Vector</p>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGGCCGGC
 
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
 
-
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
 
-
GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
 
-
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA
 
-
CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG
 
-
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC
 
-
TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA
 
-
CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG
 
-
ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
 
-
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
 
-
ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG
 
-
GCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCT
 
-
GAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCC
 
-
TCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACC
 
-
GTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTAT
 
-
CGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATAC
 
-
CGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCTATA
 
-
GCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTAT
 
-
GTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAATGGTG
 
-
GAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCG
 
-
GCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAA
 
-
ACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGG
 
-
TGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAAT
 
-
TCAACAATGGGGAGATCAATTTTACCGGTTAA
 
-
 
-
 
-
 
-
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
 
-
SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
 
-
YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
 
-
NNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
 
-
MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRY
 
-
VKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSV
 
-
EELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
 
-
 
-
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/b/be/Freiburg09_His%2BFluA%2Blonglinker%2BFokA_pMA.txt">His+FluA+longlinker+FokA_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
 
-
 
-
</div><a name="19"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+FluA+longlinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/6/6c/Freiburg09_His%2BFluA%2Blonglinker%2BFokI_pMA.jpg/600px-Freiburg09_His%2BFluA%2Blonglinker%2BFokI_pMA.jpg" name="His+FluA+longlinker+Foki in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">His+FluA+longlinker+Foki in pMA Vector</p><br>
 
-
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243016">link to part registry</a>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGGCCGGC
 
-
CATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTGCCC
 
-
CGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCAAGT
 
-
GGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAGTGC
 
-
GGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAGATA
 
-
CGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACCCCG
 
-
TGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGCGGC
 
-
TACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAACTA
 
-
CATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACTGGG
 
-
ACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCCAAG
 
-
ACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCAGAA
 
-
ACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACACCG
 
-
GCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCT
 
-
GAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCC
 
-
TCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACC
 
-
GTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTAT
 
-
CGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTATAC
 
-
CGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAGCGTATT
 
-
CTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAACGTTAT
 
-
GTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGAATGGTG
 
-
GAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCG
 
-
GCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAACCATATC
 
-
ACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGG
 
-
AGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAAT
 
-
TCAACAATGGCGAGATCAATTTTACCGGTTAA
 
-
 
-
 
-
 
-
 
-
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEGK
 
-
SVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYIIG
 
-
YSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACKV
 
-
NNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
 
-
MEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMERY
 
-
VEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLSV
 
-
EELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/7/77/Freiburg09_His%2BFluA%2Blonglinker%2BFokI_pMA.txt">His+FluA+longlinker+Foki_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
 
-
 
-
 
-
 
-
 
-
</div><a name="20"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;His+FluA+Split+FokA_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/e/ef/Freiburg09_His%2BFluA%2BSplit%2BFokA_pMA.jpg/600px-Freiburg09_His%2BFluA%2BSplit%2BFokA_pMA.jpg" name="His+FluA+Split+FokA in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">His+FluA+Split+FokA in pMA Vector</p>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGGCC
 
-
GGCCATCATCATCATCATCATACCGGCGACGTGTACCACGACGGCGCCTG
 
-
CCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACCACGGCA
 
-
AGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTATGGCAAG
 
-
TGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGTGTCCAG
 
-
ATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCGCCTACC
 
-
CCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACCGTGGGC
 
-
GGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAACAAGAA
 
-
CTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGGGCCACT
 
-
GGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGCGAGGCC
 
-
AAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGACAGCCA
 
-
GAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGAACAACA
 
-
CCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATG
 
-
AATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCA
 
-
CAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCC
 
-
GTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTC
 
-
ATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACC
 
-
TGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCG
 
-
TTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCT
 
-
GATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACAT
 
-
CAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCA
 
-
AATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTG
 
-
ACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGA
 
-
AGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGG
 
-
AAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
 
-
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGHHHHHHTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTPEG
 
-
KSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNYII
 
-
GYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAACK
 
-
VNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDR
 
-
ILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQA
 
-
DEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSN
 
-
GAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/5/53/Freiburg09_His%2BFluA%2BSplit%2BFokA_pMA.txt">His+FluA+Split+FokA_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
 
-
 
-
 
-
</div><a name="21"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+Dig+longlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/8/8b/Freiburg09_Strep%2BDig%2Blonglinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BDig%2Blonglinker%2BFokA_pMA.jpg" name="Strep+Dig+longlinker+FokA in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">Strep+Dig+longlinker+FokA in pMA Vector</p>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGGCC
 
-
GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
 
-
CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
 
-
ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC
 
-
GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
 
-
GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG
 
-
CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC
 
-
ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA
 
-
CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG
 
-
GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
 
-
GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
 
-
CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA
 
-
ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCT
 
-
GGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGA
 
-
GAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATA
 
-
TCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAA
 
-
ATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACA
 
-
TCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCC
 
-
CGATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTAT
 
-
AACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAA
 
-
CCAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATC
 
-
CGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAA
 
-
GGCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAA
 
-
TGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCA
 
-
AAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGG
 
-
GAGATCAATTTTACCGGTTAA
 
-
 
-
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP
 
-
EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY
 
-
IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
 
-
CKVNNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIA
 
-
RNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGY
 
-
NLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRL
 
-
NHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/2/22/Freiburg09_Strep%2BDig%2Blonglinker%2BFokA_pMA.txt">Strep+Dig+longlinker+FokA_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
 
-
</div><a name="22"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+Dig+longlinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/6/65/Freiburg09_Strep%2BDig%2Blonglinker%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BDig%2Blonglinker%2BFokI_pMA.jpg" name="Strep+Dig+longlinker+Foki in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">Strep+Dig+longlinker+Foki in pMA Vector <br>
 
-
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243011">link to part registry</a></li>
 
-
</p>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGGCC
 
-
GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
 
-
CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
 
-
ACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACCAAGTAC
 
-
GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
 
-
GTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGGGCACCG
 
-
CCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCTACACC
 
-
ATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCACCGACAA
 
-
CAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACAAGAAGG
 
-
GCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
 
-
GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
 
-
CAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCAAAGTGA
 
-
ACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGTGGTTCT
 
-
GGTGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGA
 
-
GAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATA
 
-
TCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAA
 
-
ATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACA
 
-
TCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCC
 
-
CGATCGATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTAT
 
-
AATCTGCCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAA
 
-
TCAGACCCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATC
 
-
CGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAA
 
-
GGCAACTATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAA
 
-
TGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCA
 
-
AAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGC
 
-
GAGATCAATTTTACCGGTTAA
 
-
 
-
 
-
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEYTP
 
-
EGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNKNY
 
-
IIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
 
-
CKVNNSNWSHPQFEKTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIA
 
-
RNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGY
 
-
NLPIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRL
 
-
NHITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/4/4c/Freiburg09_Strep%2BDig%2Blonglinker%2BFokI_pMA.txt">Strep+Dig+longlinker+Foki_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
 
-
 
-
 
-
</div><a name="23"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+Dig+middellinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/9/94/Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokA_pMA.jpg" name="Strep+Dig+middellinker+FokA in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">Strep+Dig+middellinker+FokA in pMA Vector</p>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 
-
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 
-
AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC
 
-
AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 
-
GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG
 
-
GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 
-
TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC
 
-
CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA
 
-
AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 
-
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 
-
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA
 
-
AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT
 
-
GGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAA
 
-
ATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAAC
 
-
TGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAA
 
-
GTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGG
 
-
TGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCG
 
-
ATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTG
 
-
CCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGAC
 
-
TCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAA
 
-
GCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAAC
 
-
TATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGC
 
-
CGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCG
 
-
GGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATC
 
-
AATTTTACCGGTTAA
 
-
 
-
 
-
 
-
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY
 
-
TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK
 
-
NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 
-
AACKVNNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARN
 
-
STQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNL
 
-
PIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNH
 
-
KTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
 
-
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/9/9f/Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokA_pMA.txt">Strep+Dig+middellinker+FokA_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
 
-
 
-
 
-
</div><a name="24"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+Dig+middellinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/7/79/Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokI_pMA.jpg" name="Strep+Dig+middellinker+Foki in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">Strep+Dig+middellinker+Foki in pMA Vector<br>
 
-
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243012">link to part registry</a>
 
-
</p>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 
-
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 
-
AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC
 
-
AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 
-
GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG
 
-
GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 
-
TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC
 
-
CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA
 
-
AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 
-
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 
-
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA
 
-
AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT
 
-
GGTTCTGGTGGTGGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAA
 
-
ATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAAC
 
-
TGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAA
 
-
GTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGG
 
-
TGGTAGCCGTAAACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCG
 
-
ATTATGGCGTTATCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTG
 
-
CCGATTGGTCAGGCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGAC
 
-
CCGTAACAAACATCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAA
 
-
GTGTCACCGAGTTCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAAC
 
-
TATAAAGCCCAGCTGACTCGTCTGAACCATATCACCAATAGCAATGGGGC
 
-
AGTGCTGAGTGTTGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAG
 
-
GCACCCTGACTCTGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATC
 
-
AATTTTACCGGTTAA
 
-
 
-
 
-
 
-
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY
 
-
TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK
 
-
NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 
-
AACKVNNSNWSHPQFEKTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARN
 
-
STQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNL
 
-
PIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNH
 
-
ITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/7/73/Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokI_pMA.txt">Strep+Dig+middellinker+Foki_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
 
-
 
-
 
-
</div><a name="25"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+Split+FokA_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/9/97/Freiburg09_Strep%2BFluA%2BSplit%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2BSplit%2BFokA_pMA.jpg" name="Strep+FluA+Split+FokA in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">Strep+FluA+Split+FokA in pMA Vector</p>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 
-
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 
-
AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
 
-
AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 
-
GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
 
-
GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 
-
CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
 
-
CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
 
-
AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 
-
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 
-
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
 
-
AAGTGAACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAG
 
-
CAGAAAGTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATC
 
-
CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA
 
-
TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG
 
-
ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG
 
-
TAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATT
 
-
ATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCA
 
-
ATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCG
 
-
TAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCG
 
-
TTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTAT
 
-
AAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGT
 
-
TCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGA
 
-
CCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAAT
 
-
TTTACCGGTTAA
 
-
 
-
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
 
-
TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
 
-
NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 
-
AACKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS
 
-
TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLP
 
-
IGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHK
 
-
TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/7/73/Freiburg09_Strep%2BDig%2Bmiddellinker%2BFokI_pMA.txt">Strep+Dig+middellinker+Foki_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
 
-
 
-
 
-
</div><a name="26"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+Split+FokA_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/9/97/Freiburg09_Strep%2BFluA%2BSplit%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2BSplit%2BFokA_pMA.jpg" name="Strep+FluA+Split+FokA in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">Strep+FluA+Split+FokA in pMA Vector</p>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 
-
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 
-
AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
 
-
AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 
-
GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
 
-
GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 
-
CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
 
-
CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
 
-
AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 
-
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 
-
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
 
-
AAGTGAACAACACCGGCCGACCAGCCTGTAAGATTCCAAATGACCTGAAG
 
-
CAGAAAGTTATGAATCACACCGGCAAATCTGAACTGGAGGAGAAAAAATC
 
-
CGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGA
 
-
TCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTG
 
-
ATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGG
 
-
TAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATT
 
-
ATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCA
 
-
ATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCG
 
-
TAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCG
 
-
TTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTAT
 
-
AAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGT
 
-
TCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGA
 
-
CCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAAT
 
-
TTTACCGGTTAA
 
-
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
 
-
TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
 
-
NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 
-
AACKVNNTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEYIELIEIARNS
 
-
TQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLP
 
-
IGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHK
 
-
TNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/6/6a/Freiburg09_Strep%2BFluA%2BSplit%2BFokA_pMA.txt">Strep+FluA+Split+FokA_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
 
-
 
-
</div><a name="27"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+Dig+shortlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/f/fb/Freiburg09_Strep%2BDig%2Bshortlinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BDig%2Bshortlinker%2BFokA_pMA.jpg" name="Strep+Dig+shortlinker+FokA in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">Strep+Dig+shortlinker+FokA in pMA Vector</p>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 
-
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 
-
AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC
 
-
AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 
-
GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG
 
-
GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 
-
TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC
 
-
CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA
 
-
AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 
-
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 
-
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA
 
-
AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT
 
-
GGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCG
 
-
CCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCG
 
-
CCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTC
 
-
TTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAA
 
-
ACCTGATGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTA
 
-
TCGTTGATACCAAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAG
 
-
GCTGATGAGATGCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACA
 
-
CATCAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGT
 
-
TCAAATTCCTGTTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAG
 
-
CTGACCCGTCTGAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGT
 
-
AGAAGAGCTGCTGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACAC
 
-
TGGAAGAAGTTCGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGT
 
-
TAA
 
-
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY
 
-
TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK
 
-
NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 
-
AACKVNNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQD
 
-
RILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQ
 
-
ADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNS
 
-
NGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/4/45/Freiburg09_Strep%2BDig%2Bshortlinker%2BFokA_pMA.txt">Strep+Dig+shortlinker+FokA_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
 
-
 
-
</div><a name="28"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+Dig+shortlinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/4/4a/Freiburg09_Strep%2BDig%2Bshortlinker%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BDig%2Bshortlinker%2BFokI_pMA.jpg" name="Strep+Dig+shortlinker+Foki in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">Strep+Dig+shortlinker+Foki in pMA Vector</p>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 
-
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 
-
AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC
 
-
AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 
-
GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG
 
-
GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 
-
TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC
 
-
CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA
 
-
AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 
-
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 
-
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA
 
-
AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCGGT
 
-
GGTTCTGGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCG
 
-
CCACAAACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCG
 
-
CCCGTAATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTC
 
-
TTCATGAAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAA
 
-
ACCAGCAGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTA
 
-
TCGTGGCCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAG
 
-
GCTGATGAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACA
 
-
TCTGAACCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGT
 
-
TCAAATTTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAG
 
-
CTGACTCGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGT
 
-
TGAGGAACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTC
 
-
TGGAAGAAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGT
 
-
TAA
 
-
 
-
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY
 
-
TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK
 
-
NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 
-
AACKVNNSNWSHPQFEKTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQD
 
-
RILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQ
 
-
ADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNS
 
-
NGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/0/0f/Freiburg09_Strep%2BDig%2Bshortlinker%2BFokI_pMA.txt">Strep+Dig+shortlinker+Foki_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
 
-
 
-
 
-
</div><a name="29"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+Dig+Split+FokA_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/f/ff/Freiburg09_Strep%2BDig%2BSplit%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BDig%2BSplit%2BFokA_pMA.jpg" name="Strep+Dig+Split+FokA in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">Strep+Dig+Split+FokA in pMA Vector</p><br>
 
-
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243017">link to part registry</a>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 
-
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 
-
AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC
 
-
AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 
-
GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG
 
-
GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 
-
TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC
 
-
CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA
 
-
AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 
-
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 
-
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA
 
-
AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGA
 
-
CCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACAC
 
-
CGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGA
 
-
AATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGT
 
-
ACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGT
 
-
CTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTG
 
-
CCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACC
 
-
AAAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGAT
 
-
GCAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGA
 
-
ACGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTG
 
-
TTCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCT
 
-
GAACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGC
 
-
TGATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTT
 
-
CGCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
 
-
 
-
 
-
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY
 
-
TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK
 
-
NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 
-
AACKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEY
 
-
IELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDT
 
-
KAYSGGYNLPIGQADEMQRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNY
 
-
KAQLTRLNHKTNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/9/95/Freiburg09_Strep%2BDig%2BSplit%2BFokA_pMA.txt">Strep+Dig+Split+FokA_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
 
-
 
-
 
-
</div><a name="30"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+Dig+Split+Foki_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/f/fe/Freiburg09_Strep%2BDig%2BSplit%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BDig%2BSplit%2BFokI_pMA.jpg" name="Strep+Dig+Split+Foki in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">Strep+Dig+Split+Foki in pMA Vector</p><br>
 
-
<li> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K243015">link to part registry</a>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 
-
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 
-
AGTACCACGGCAAGTGGTGGCAGGTGGCCGCTTATCCCGACCACATCACC
 
-
AAGTACGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 
-
GAAGGTGTCCCGGTACAGCGTGATCCACGGCAAAGAGTACTTCAGCGAGG
 
-
GCACCGCCTACCCTGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 
-
TACACCATCGGCGGCGTGACCCAGGTGGGCGTGAGCAACGTGCTGTCCAC
 
-
CGACAACAAGAACTACATCATCGGCTACTTTTGCAGATACGACGAGGACA
 
-
AGAAGGGCCACTGGGACGCCGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 
-
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 
-
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCTCCGAGGCCGCCTGCA
 
-
AAGTGAACAACAGCAACTGGTCCCACCCCCAGTTCGAAAAGACCGGCCGA
 
-
CCAGCCTGTAAGATTCCAAATGACCTGAAGCAGAAAGTTATGAATCACAC
 
-
CGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGA
 
-
AATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGT
 
-
ACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGT
 
-
CTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTG
 
-
CCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACA
 
-
AAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGAT
 
-
GGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGA
 
-
ACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTG
 
-
TTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCT
 
-
GAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGC
 
-
TGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTT
 
-
CGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA
 
-
 
-
 
-
 
-
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWQVAAYPDHITKYGKCGWAEY
 
-
TPEGKSVKVSRYSVIHGKEYFSEGTAYPVGDSKIGKIYHSYTIGGVTQVGVSNVLSTDNK
 
-
NYIIGYFCRYDEDKKGHWDAVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 
-
AACKVNNSNWSHPQFEKTGRPACKIPNDLKQKVMNHTGKSELEEKKSELRHKLKYVPHEY
 
-
IELIEIARNSTQDRILEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVAT
 
-
KAYSGGYNLPIGQADEMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNY
 
-
KAQLTRLNHITNSNGAVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/6/63/Freiburg09_Strep%2BDig%2BSplit%2BFokI_pMA.txt">Strep+Dig+Split+Foki_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
 
-
 
-
</div><a name="31"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+longlinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/2/22/Freiburg09_Strep%2BFluA%2Blonglinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2Blonglinker%2BFokA_pMA.jpg" name="Strep+FluA+longlinker+FokA in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">Strep+FluA+longlinker+FokA in pMA Vector</p>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
GGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCACGACGG
 
-
CGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCCAGTACC
 
-
ACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGCAAGTAT
 
-
GGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGTGAAGGT
 
-
GTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAGGCACCG
 
-
CCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGCCGGACC
 
-
GTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCACCGACAA
 
-
CAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACAAGAAGG
 
-
GCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTGACCGGC
 
-
GAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGTGGTGGA
 
-
CAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCAAAGTGA
 
-
ACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCTGGTACC
 
-
GGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAA
 
-
ATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTA
 
-
CCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTC
 
-
TATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGC
 
-
CATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCA
 
-
AAGCCTATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATG
 
-
CAGCGTTATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAA
 
-
CGAATGGTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGT
 
-
TCGTGAGCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTG
 
-
AACCATAAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCT
 
-
GATTGGCGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTC
 
-
GCCGTAAATTCAACAATGGGGAGATCAATTTTACCGGTTAA
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
GWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEYTP
 
-
EGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNKNY
 
-
IIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSEAA
 
-
CKVNNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILE
 
-
MKVMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEM
 
-
QRYVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAV
 
-
LSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/d/d4/Freiburg09_Strep%2BFluA%2Blonglinker%2BFokA_pMA.txt">Strep+FluA+longlinker+FokA_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
 
-
 
-
</div><a name="32"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+longlinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/4/47/Freiburg09_Strep%2BFluA%2Blonglinker%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2Blonglinker%2BFokI_pMA.jpg" name="Strep+FluA+longlinker+Foki in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">Strep+FluA+longlinker+Foki in pMA Vector</p>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 
-
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 
-
AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
 
-
AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 
-
GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
 
-
GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 
-
CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
 
-
CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
 
-
AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 
-
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 
-
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
 
-
AAGTGAACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTGGTGGTTCT
 
-
GGTACCGGCAAATCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAA
 
-
ACTGAAATATGTGCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTA
 
-
ATAGTACCCAAGACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATG
 
-
AAAGTCTATGGCTATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGC
 
-
AGGTGCCATTTATACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGG
 
-
CCACAAAAGCGTATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGAT
 
-
GAGATGGAACGTTATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAA
 
-
CCCGAACGAATGGTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAAT
 
-
TTCTGTTCGTGAGCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACT
 
-
CGTCTGAACCATATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGA
 
-
ACTGCTGATCGGTGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAG
 
-
AAGTTCGCCGTAAATTCAACAATGGCGAGATCAATTTTACCGGTTAA
 
-
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
 
-
TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
 
-
NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 
-
AACKVNNTGGGSGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRI
 
-
LEMKVMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQAD
 
-
EMERYVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNG
 
-
AVLSVEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/c/ce/Freiburg09_Strep%2BFluA%2Blonglinker%2BFokI_pMA.txt">Strep+FluA+longlinker+Foki_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
 
-
 
-
</div><a name="33"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+middellinker+FokA_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/d/d8/Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokA_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokA_pMA.jpg" name="Strep+FluA+middellinker+FokA in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">Strep+FluA+middellinker+FokA in pMA Vector</p>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 
-
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 
-
AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
 
-
AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 
-
GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
 
-
GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 
-
CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
 
-
CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
 
-
AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 
-
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 
-
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
 
-
AAGTGAACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAA
 
-
TCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGT
 
-
GCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAG
 
-
ACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGC
 
-
TATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTA
 
-
TACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCT
 
-
ATAGCGGGGGTTATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGT
 
-
TATGTGAAAGAGAACCAGACTCGTAACAAACACATCAACCCGAACGAATG
 
-
GTGGAAAGTGTATCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGA
 
-
GCGGCCATTTTAAAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCAT
 
-
AAAACCAATAGCAATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGG
 
-
CGGTGAAATGATCAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTA
 
-
AATTCAACAATGGGGAGATCAATTTTACCGGTTAA
 
-
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
 
-
TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
 
-
NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 
-
AACKVNNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMK
 
-
VMEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
 
-
YVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLS
 
-
VEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/4/48/Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokA_pMA.txt">Strep+FluA+middellinker+FokA_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
 
-
 
-
</div><a name="34"></a>
 
-
<h3 class="art-PostHeaderIcon-wrapper"> &nbsp;Strep+FluA+middellinker+Foki_pMA<span class="art-PostHeader"></span> </h3>
 
-
</div>
 
-
<div class="art-PostContent">
 
-
<div style="text-align: center;"><font size="1"><a href="#1">back to index</a></font>
 
-
 
-
 
-
<table border="5">
 
-
<tr>
 
-
<td align="center">
 
-
<img src="https://static.igem.org/mediawiki/2009/thumb/b/b9/Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokI_pMA.jpg/600px-Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokI_pMA.jpg" name="Strep+FluA+middellinker+Foki in pMA Vector" width="480" height="480" />
 
-
<br><p align="center">Strep+FluA+middellinker+Foki in pMA Vector</p>
 
-
</td>
 
-
<td align="left">
 
-
nucleotid sequence of ORF-1:<br>
 
-
 
-
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA
 
-
CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC
 
-
AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC
 
-
AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT
 
-
GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG
 
-
GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC
 
-
CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC
 
-
CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA
 
-
AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG
 
-
ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT
 
-
GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA
 
-
AAGTGAACAACACCGGCGGTGGTTCTGGTGGTGGTTCTGGTACCGGCAAA
 
-
TCTGAACTGGAGGAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGT
 
-
GCCTCACGAGTATATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAG
 
-
ACCGTATCCTGGAAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGC
 
-
TATCGTGGCAAACATCTGGGTGGTAGCCGTAAACCAGCAGGTGCCATTTA
 
-
TACCGTTGGTTCCCCGATCGATTATGGCGTTATCGTGGCCACAAAAGCGT
 
-
ATTCTGGCGGTTATAATCTGCCGATTGGTCAGGCTGATGAGATGGAACGT
 
-
TATGTGGAAGAGAATCAGACCCGTAACAAACATCTGAACCCGAACGAATG
 
-
GTGGAAAGTGTATCCGTCAAGTGTCACCGAGTTCAAATTTCTGTTCGTGA
 
-
GCGGCCACTTTAAAGGCAACTATAAAGCCCAGCTGACTCGTCTGAACCAT
 
-
ATCACCAATAGCAATGGGGCAGTGCTGAGTGTTGAGGAACTGCTGATCGG
 
-
TGGAGAAATGATCAAAGCAGGCACCCTGACTCTGGAAGAAGTTCGCCGTA
 
-
AATTCAACAATGGCGAGATCAATTTTACCGGTTAA
 
-
 
-
 
-
 
-
</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<h4>AA sequence of ORF-1:<br></h4>
 
-
<table border="0">
 
-
<p align="justify"><td align="left">
 
-
MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY
 
-
TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK
 
-
NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE
 
-
AACKVNNTGGGSGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMK
 
-
VMEFFMKVYGYRGKHLGGSRKPAGAIYTVGSPIDYGVIVATKAYSGGYNLPIGQADEMER
 
-
YVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNSNGAVLS
 
-
VEELLIGGEMIKAGTLTLEEVRRKFNNGEINFTG
 
-
 
-
 
-
 
-
<br><br></p></td></table>
 
-
 
-
<h4>Commented GenBank file</h4>
 
-
<li> <a href="https://static.igem.org/mediawiki/2009/a/a3/Freiburg09_Strep%2BFluA%2Bmiddellinker%2BFokI_pMA.txt">Strep+FluA+middellinker+Foki_pMA.txt</a></li>
 
-
<br>
 
-
<br>
 
-
<br>
 

Revision as of 12:45, 17 October 2009

FREiGEM

Index


*His+FluA+Split+Foki_pMA

* His+FluA+longlinker+Foki_pMA

 Parts

 Strep+FluA+shortlinker+FokA_pMA

back to index

Strep+FluA+shortlinker+FokA in pMA Vector

nucleotid sequence of ORF-1:
ATGGCCGGCTGGAGCCATCCGCAGTTTGAAAAAACCGGCGACGTGTACCA CGACGGCGCCTGCCCCGAAGTGAAGCCCGTGGACAACTTCGACTGGTCCC AGTACCACGGCAAGTGGTGGGAGGTGGCCAAGTACCCCAGCCCCAACGGC AAGTATGGCAAGTGCGGCTGGGCCGAGTACACCCCCGAGGGCAAGAGCGT GAAGGTGTCCAGATACGACGTGATCCACGGCAAAGAATACTTCATGGAAG GCACCGCCTACCCCGTGGGCGACAGCAAGATCGGCAAGATCTACCACAGC CGGACCGTGGGCGGCTACACCAGAAAGACCGTGTTCAACGTGCTGTCCAC CGACAACAAGAACTACATCATCGGCTACAGCTGCCGCTACGACGAGGACA AGAAGGGCCACTGGGACCACGTGTGGGTGCTGTCCCGGTCCATGGTGCTG ACCGGCGAGGCCAAGACCGCCGTGGAGAACTACCTGATCGGCAGCCCCGT GGTGGACAGCCAGAAACTGGTGTACAGCGACTTCAGCGAGGCCGCCTGCA AAGTGAACAACACCGGCGGTGGTTCTGGTACCGGCAAATCTGAACTGGAG GAGAAAAAATCCGAGCTGCGCCACAAACTGAAATATGTGCCTCACGAGTA TATCGAACTGATCGAGATCGCCCGTAATAGTACCCAAGACCGTATCCTGG AAATGAAAGTGATGGAGTTCTTCATGAAAGTCTATGGCTATCGTGGCAAA CATCTGGGTGGTAGCCGTAAACCTGATGGTGCCATTTATACCGTTGGTTC CCCGATCGATTATGGCGTTATCGTTGATACCAAAGCCTATAGCGGGGGTT ATAACCTGCCAATTGGTCAGGCTGATGAGATGCAGCGTTATGTGAAAGAG AACCAGACTCGTAACAAACACATCAACCCGAACGAATGGTGGAAAGTGTA TCCGTCAAGCGTTACAGAGTTCAAATTCCTGTTCGTGAGCGGCCATTTTA AAGGCAACTATAAAGCACAGCTGACCCGTCTGAACCATAAAACCAATAGC AATGGCGCCGTTCTGTCAGTAGAAGAGCTGCTGATTGGCGGTGAAATGAT CAAAGCCGGGACCCTGACACTGGAAGAAGTTCGCCGTAAATTCAACAATG GGGAGATCAATTTTACCGGTTAA

AA sequence of ORF-1:

MAGWSHPQFEKTGDVYHDGACPEVKPVDNFDWSQYHGKWWEVAKYPSPNGKYGKCGWAEY TPEGKSVKVSRYDVIHGKEYFMEGTAYPVGDSKIGKIYHSRTVGGYTRKTVFNVLSTDNK NYIIGYSCRYDEDKKGHWDHVWVLSRSMVLTGEAKTAVENYLIGSPVVDSQKLVYSDFSE AACKVNNTGGGSGTGKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVMEF FMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRYVKE NQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNSNGAVLSVEEL LIGGEMIKAGTLTLEEVRRKFNNGEINFTG

Commented GenBank file

  • Strep+FluA+shortlinker+FokA_pMA.txt





  • text here




    Retrieved from "http://2009.igem.org/Team:Freiburg_bioware/cloning1"