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Team:Berkeley Wetlab/Recipes
From 2009.igem.org
(Difference between revisions)
| Line 25: | Line 25: | ||
2% SDS<br> | 2% SDS<br> | ||
pH 7<br> | pH 7<br> | ||
| + | |||
| + | ====Precipitation Buffer==== | ||
| + | 2 mL of acetic acid<br> | ||
| + | Add concentrated NaOH until the pH is 5 | ||
| + | Add water to reach 10 mL total volume volume | ||
| + | 0.2 g ZnCl | ||
| + | Add 40 mL ethanol | ||
'''*For our Assay Protocols Visit the Passengers Tab''' | '''*For our Assay Protocols Visit the Passengers Tab''' | ||
Revision as of 20:49, 18 October 2009
|
Contents |
10X TBST
31.4 g TRIS HCl
80g NaCl
10ml Tween-20
bring volume to 1000ml
pH 7.5
1X TBS
605 mg Tris
876 mg NaCl
80 ml H2O
pH 7.4
bring volume to 100 ml
Artificial Seawater
500 ml ddH2O
12.2 g NaCl
2.6 g MgCl2
.57 g CaCl2
Tris Buffer + 2% SDS
50 mM Tris
150 mM NaCl
2% SDS
pH 7
Precipitation Buffer
2 mL of acetic acid
Add concentrated NaOH until the pH is 5
Add water to reach 10 mL total volume volume
0.2 g ZnCl
Add 40 mL ethanol
*For our Assay Protocols Visit the Passengers Tab
