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Template:Team:KULeuven/12 August 2009/VanillinProduction
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JochemDeen (Talk | contribs) (New page: *miniprep * restriction digest on Sam5, Sam8 and Fcs ** There was no growth on Ech, thus new cultures in liquid medium * Gel electrophorese on Sam5, Sam8 and Fcs * Gel extraction on Sam5 a...) |
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| - | *miniprep | + | * miniprep on Sam5, Sam8 and Fcs |
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** There was no growth on Ech, thus new cultures in liquid medium | ** There was no growth on Ech, thus new cultures in liquid medium | ||
| + | * restriction digest on Sam5, Sam8 and Fcs | ||
* Gel electrophorese on Sam5, Sam8 and Fcs | * Gel electrophorese on Sam5, Sam8 and Fcs | ||
* Gel extraction on Sam5 and Fcs | * Gel extraction on Sam5 and Fcs | ||
Revision as of 14:42, 12 August 2009
- miniprep on Sam5, Sam8 and Fcs
- There was no growth on Ech, thus new cultures in liquid medium
- restriction digest on Sam5, Sam8 and Fcs
- Gel electrophorese on Sam5, Sam8 and Fcs
- Gel extraction on Sam5 and Fcs
Nanodrop results
| Part | concentration (ng/μl) | 260/280 λ | 260/230 λ |
|---|---|---|---|
| Fcs | 4,2 | 6,08 | |
| Sam5 | 7,3 | 2,76 |
Notes:
- Sam8 showed a very low concentration on the gel electrophorese, thus better to do this again.
- 260/280 for Fcs was very high, also the concentration was not too high, better to redo the extraction and use multiple tubes on the same filter to increase the concentration
