Team:BIOTEC Dresden/Results Vesicles

From 2009.igem.org

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[[Image:Our_1st_GUVs.jpg|500px]]
[[Image:Our_1st_GUVs.jpg|500px]]
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unfortunately, this method only produced that were unstable and had a wide size distribution.
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unfortunately, this method mostly produced GUVs that were unstable and had a wide size distribution.
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''Second approach: Mixing in Microfluidic Chamber''
''Second approach: Mixing in Microfluidic Chamber''
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And those pretty pictures were created, following the second protocol.
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A microfluidic chamber that created an intersection between aqueous and oil phases produced vesicles that were uniform in size and stable for up to 5 hours.  

Revision as of 18:47, 19 October 2009

Lipid Vesicles

First approach: Microjetting

Our first GUVs were made by shooting aqueous material through a lipid film using a microjetting setup.

Our 1st GUVs.jpg

unfortunately, this method mostly produced GUVs that were unstable and had a wide size distribution.


Second approach: Mixing in Microfluidic Chamber

A microfluidic chamber that created an intersection between aqueous and oil phases produced vesicles that were uniform in size and stable for up to 5 hours.


vesicles created in microfluid chamber