September/25 September 2009

From 2009.igem.org

(Difference between revisions)
(New page: 1.colony check<br> sample no. no. of colony 2-18B >50 53 >50 X5 >50 X6 >10 X7 2 X8 5 <br> 2.min...)
 
Line 80: Line 80:
  sample no
  sample no
   55 , 54
   55 , 54
 +
 +
[https://2009.igem.org/Team:Osaka/NOTES back to NOTES]

Latest revision as of 11:12, 20 October 2009

1.colony check

sample no.   no. of colony
  2-18B          >50
   53            >50
   X5            >50
   X6            >10
   X7             2
   X8             5


2.min prep
result of nano drop.

sample no.      conc.
   11          309 ng/uL
  3-1M         14
   28          260
   29          241
   30          219
   39          188
   46          224
   52          218
  X4-A         196
  X4-B         184


3.transformation

sample no. 37


4.digation


K204054

VectorInsert
2-16L10283
EcoRI1EcoRI1
XbaI1SpeI1
No.22No.22
dH2O6dH2O13
total20uLtotal20uL


K204055

VectorInsert
294286
EcoRI1EcoRI1
XbaI1SpeI1
No.22No.22
dH2O12dH2O10
total20uLtotal20uL



K204056

VectorInsert
1-23L12303
EcoRI1EcoRI1
XbaI1SpeI1
No.22No.22
dH2O4dH2O13
total20uLtotal20uL


37degree , 3hr
Agarose Gel Electro-phoresis

gel cut
purification
ligation



5.transformation

sample no
  55 , 54

back to NOTES