Team:USTC/Standard & Protocol

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===Transformation===
===Transformation===
===Colony PCR===
===Colony PCR===
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==Measurement protocol==

Revision as of 16:57, 20 October 2009

USTC
Home Team Project Modeling Parts Standard & Protocol Software Tool Human Practice Notebook

Team:USTC/Standard & Protocol

Contents

Standard protocol

Minipreps

Performed with BIO BASIC INC. EZ-10 Spin Column Plasmid DNA MiniPreps Kit BS414

Digestion

The digestion enzymes we use are listed:

Pst I Fermentas ER0611 3000U

EcoR I Fermentas ER0271 5000U

Spe I Fermentas ER1251 1500U

Xba I Fermentas ER0681 400U

Gel Extraction

Performed with BIO BASIC INC. EZ-10 Spin Column DNA Gel Extraction Kit BS354

Ligation

For short segments:

TakaRa DNA Ligation Kit Ver2.0 Code D6022

BIO BASIC INC. FAST LIGATION KIT BS512

For long segments ligation:

TaKaRa DNA Ligation Kit LONG Code D6024

Transformation

Colony PCR

Measurement protocol