Virginia Commonwealth/9 July 2009

From 2009.igem.org

(Difference between revisions)
(Tasks)
(Tasks)
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===Tasks===
===Tasks===
''Afton and Maria''
''Afton and Maria''
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* Pick cells for overnight culture
+
* Pick cells for overnight culture  
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* Make a glycerol stock to go in the -80 degree Celsius freezer
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[[User:Trentay|Trentay]] 19:45, 9 July 2009 (UTC)
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[[User:Trentay|Trentay]] 14:06, 9 July 2009 (UTC)
+
 
''Kevin and Adam''
''Kevin and Adam''
* Back bone pSB3K3 is a bad part. Cultivate backbone pSB1C3 from glycerol stock.
* Back bone pSB3K3 is a bad part. Cultivate backbone pSB1C3 from glycerol stock.

Revision as of 19:45, 9 July 2009

Contents

Thursday 9 July 2009

Results

Afton and Maria

  • Electrotransformation was successful
    • Control (+) NEB 10 beta on LB has lawn growth
    • Control (+) NEB 10 beta on LB electroshocked has lawn growth
    • Control (-) NEB 10 beta on AMP has no growth
    • J23106 (promoter) on AMP has hundreds of little colonies
    • J06702 (RET) on AMP has about one hundred colonies

Trentay 14:06, 9 July 2009 (UTC)


Tasks

Afton and Maria

  • Pick cells for overnight culture

Trentay 19:45, 9 July 2009 (UTC)

Kevin and Adam

  • Back bone pSB3K3 is a bad part. Cultivate backbone pSB1C3 from glycerol stock.
  • Proceed with analytical work and ordering DNA.

Wetlab

Afton and Maria

  • 1
  • 2

Trentay 14:06, 9 July 2009 (UTC)