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Virginia Commonwealth/9 July 2009
From 2009.igem.org
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''Afton and Maria'' | ''Afton and Maria'' | ||
| - | * | + | * Put 5 colonies of J23106 in 5mL of LB (two test tube) |
| - | * | + | * Put 5 colonies of J06702 in 5mL of LB (one test tube) |
| - | [[User:Trentay|Trentay]] | + | [[User:Trentay|Trentay]] 21:13, 9 July 2009 (UTC) |
Revision as of 21:13, 9 July 2009
Contents |
Thursday 9 July 2009
Results
Afton and Maria
- Electrotransformation was successful
- Control (+) NEB 10 beta on LB has lawn growth
- Control (+) NEB 10 beta on LB electroshocked has lawn growth
- Control (-) NEB 10 beta on AMP has no growth
- J23106 (promoter) on AMP has hundreds of little colonies
- J06702 (RET) on AMP has about one hundred colonies
Trentay 14:06, 9 July 2009 (UTC)
Tasks
Afton and Maria
- Pick cells for overnight culture
Trentay 19:45, 9 July 2009 (UTC)
Kevin and Adam
- Back bone pSB3K3 is a bad part. Cultivate backbone pSB1C3 from glycerol stock.
- Proceed with analytical work and ordering DNA.
Wetlab
Afton and Maria
- Put 5 colonies of J23106 in 5mL of LB (two test tube)
- Put 5 colonies of J06702 in 5mL of LB (one test tube)
Trentay 21:13, 9 July 2009 (UTC)
