Team:Wash U/Calendar-Home/14 July 2009

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(Difference between revisions)

Revision as of 21:30, 14 July 2009

Tuesday, July 14, 2009

-Colony PCR of cells transformed with previous ligations
-PCR to add KpnI and BamHI sites to GFP
-Spectrophotometry of dbcOmega strains in ATCC media
-PCR to add BioBrick prefix and suffix to Cph8 and pRKCBC3
-Transformed S17 competent cells to check efficiency. OD = 0.115
-Performed Triligation and Silver Lab Ligations to fuse OmpC+GFP
-Started cultures of dbcOmega in LB+Strep in lieu of ATCC

Gel #1: Silver digestion protocol

1) Ladder

2) #1 OmpC (108 bp)

3) #1 OmpC

4) #1 GFP (876 bp)

5) #1 GFP

6) Ladder

Gel #2: BioBrick digestion protocol

1) Ladder

2) #2 OmpC

3) #2 OmpC

4) #2 Destination plasmid (pSB1K3)

5) #2 Dest. plasmid (pSB1K3)

6) #2 GFP

7) #2 GFP

8) Ladder

Back to Calendar

@@ Line 10: / Line 10: @@
 -Started cultures of dbcOmega in LB+Strep in lieu of ATCC <br>
-Gel #1: Silver
+Gel #1: Silver digestion protocol
 ) Ladder
-) #1 OmpC
+) #1 OmpC (108 bp)
 ) #1 OmpC
-) #1 GFP
+) #1 GFP (876 bp)
 ) #1 GFP
@@ Line 24: / Line 24: @@
 ) Ladder
-Gel #2: BioBrick
+Gel #2: BioBrick digestion protocol
 ) Ladder