Virginia Commonwealth/4 August 2009

From 2009.igem.org

(Difference between revisions)
(Tuesday 4 August 2009)
(Tuesday 4 August 2009)
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** Sample taken from plate after PSB4C5 ligation attempt successfully grew expressing RFP. The color of the sample was pink
** Sample taken from plate after PSB4C5 ligation attempt successfully grew expressing RFP. The color of the sample was pink
** Sample taken from plate after ligation with itself did not grow at all, and expressed no RFP
** Sample taken from plate after ligation with itself did not grow at all, and expressed no RFP
-
[[User:Trentay|Trentay]] 22:11, 4 August 2009 (UTC)
+
* There is a possibility the pSB4C5 backbone may be a bad part
 +
** previous years it has not functioned properly
 +
** Gel electrophoresis indicates DNA Gel Purification may be necessary
 +
* Possible inefficiency of BioBrick enzymes may have come from repeated freezing and thawing
 +
** They will be aliquoted into smaller amounts
 +
[[User:Trentay|Trentay]] 22:25, 4 August 2009 (UTC)
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===Tasks===
===Tasks===
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''Maria and Afton''
''Maria and Afton''
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*
+
* Gel Electrophoresis was run
 +
** pSB4C5 and I1352 digested and undigested (right after Miniprep) were run on a gel and results showed excessive bands in the undigested DNA. DNA Gel Purification will be done on future Miniprepped DNA

Revision as of 22:25, 4 August 2009

Contents

Tuesday 4 August 2009

Results

Maria and Afton

  • Overnight culture of I1352 plated on AMP:
    • Sample taken from plate after PSB4C5 ligation attempt successfully grew expressing RFP. The color of the sample was pink
    • Sample taken from plate after ligation with itself did not grow at all, and expressed no RFP
  • There is a possibility the pSB4C5 backbone may be a bad part
    • previous years it has not functioned properly
    • Gel electrophoresis indicates DNA Gel Purification may be necessary
  • Possible inefficiency of BioBrick enzymes may have come from repeated freezing and thawing
    • They will be aliquoted into smaller amounts

Trentay 22:25, 4 August 2009 (UTC)


Tasks

Maria and Afton

  • Run a Gel Electrophoresis
  • Transform and grow up pSB1C3 with the death gene P1010

Trentay 22:11, 4 August 2009 (UTC)


Wetlab

  • New BioBrick Assembly Kit came in and the following were aliquoted into their respective amounts
    • XbaI (10 µL)
    • SpeI (5 µL)
    • PstI (10 µL)
    • EcoRI-HF (10 µL)
    • BSA (5 µL)
    • T4 Ligase Buffer (10 µL)
    • T4 Ligase (5 µL)

Maria and Afton

  • Gel Electrophoresis was run
    • pSB4C5 and I1352 digested and undigested (right after Miniprep) were run on a gel and results showed excessive bands in the undigested DNA. DNA Gel Purification will be done on future Miniprepped DNA