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Virginia Commonwealth/4 August 2009
From 2009.igem.org
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* pSB1C3 w/ P1010 was removed from Well 5E on Plate 1 of the Distribution Kit and resuspended in 10µL of TE Buffer. | * pSB1C3 w/ P1010 was removed from Well 5E on Plate 1 of the Distribution Kit and resuspended in 10µL of TE Buffer. | ||
* Transformation was done using DB 3.1 | * Transformation was done using DB 3.1 | ||
| - | {| | + | {|border="1" |
|+ | |+ | ||
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|- | |- | ||
! pSB1C3 | ! pSB1C3 | ||
| - | | CM|| | + | | CM|| n/a || |
|- | |- | ||
! (+) Control | ! (+) Control | ||
| - | | LB || | + | | LB || n/a || |
|- | |- | ||
! (+) Control zapped | ! (+) Control zapped | ||
| - | | LB || | + | | LB || n/a || |
|- | |- | ||
! (-) Control | ! (-) Control | ||
| - | | CM || | + | | CM || n/a || |
|} | |} | ||
[[User:Trentay|Trentay]] 22:33, 4 August 2009 (UTC) | [[User:Trentay|Trentay]] 22:33, 4 August 2009 (UTC) | ||
Revision as of 13:38, 5 August 2009
Contents |
Tuesday 4 August 2009
Results
Maria and Afton
- Overnight culture of I1352 plated on AMP:
- Sample taken from plate after PSB4C5 ligation attempt successfully grew expressing RFP. The color of the sample was pink
- Sample taken from plate after ligation with itself did not grow at all, and expressed no RFP
- There is a possibility the pSB4C5 backbone may be a bad part
- previous years it has not functioned properly
- Gel electrophoresis indicates DNA Gel Purification may be necessary
- Possible inefficiency of BioBrick enzymes may have come from repeated freezing and thawing
- They will be aliquoted into smaller amounts
Trentay 22:25, 4 August 2009 (UTC)
Tasks
Maria and Afton
- Run a Gel Electrophoresis
- Transform and grow up pSB1C3 with the death gene P1010
Trentay 22:11, 4 August 2009 (UTC)
Wetlab
- New BioBrick Assembly Kit came in and the following were aliquoted into their respective amounts
| Name | Amount/Tube | # Tubes | Extra in Stock | |
|---|---|---|---|---|
| XbaI | 10 µL | 15 | No | |
| SpeI | 5 µL | 10 | No | |
| PstI | 10 µL | 25 | Yes | |
| EcoRI-HF | 10 µL | 25 | Yes | |
| BSA | 5 µL | 8 | Yes | |
| T4 Ligase Buffer | 10 µL | 25 | Yes | |
| T4 Ligase | 10 µL | 8 | No |
Trentay 22:33, 4 August 2009 (UTC)
Maria and Afton
- Gel Electrophoresis was run
- pSB4C5 and I1352 digested and undigested (right after Miniprep) were run on a gel and results showed excessive bands in the undigested DNA. DNA Gel Purification will be done on future Miniprepped DNA
- pSB1C3 w/ P1010 was removed from Well 5E on Plate 1 of the Distribution Kit and resuspended in 10µL of TE Buffer.
- Transformation was done using DB 3.1
| Name | Plate | Growth Observation | |
|---|---|---|---|
| pSB1C3 | CM | n/a | |
| (+) Control | LB | n/a | |
| (+) Control zapped | LB | n/a | |
| (-) Control | CM | n/a |
Trentay 22:33, 4 August 2009 (UTC)
