Team:Chiba/Notebook/Calendar/29 September 2009

From 2009.igem.org

(Difference between revisions)
(Gel electrophoresis)
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== Gel electrophoresis ==
== Gel electrophoresis ==
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Yesterday's oparation is [https://2009.igem.org/wiki/index.php?title=Team:Chiba/Notebook/Calender/28_September_2009 here].
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Yesterday's oparation is [https://2009.igem.org/Team:Chiba/Notebook/Calendar/28_September_2009 here].
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Gel electrophoresis again(135 V, 27 min)
Gel electrophoresis again(135 V, 27 min)
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== Digestion Test ==
== Digestion Test ==

Revision as of 07:48, 3 October 2009

>Go to the Notebook page

(28_September_2009 <|>30_September_2009)



Contents

Gel electrophoresis

Yesterday's oparation is here.


  • Today's operation

10:40-

Mini Prep.


13:30-

Gel electrophoresis(135 V, 27 min)


---DNA Clean & Concentrator---


15:50-

Gel electrophoresis again(135 V, 27 min)

Digestion Test

  • Sample

Vector(plux-GFP)


18:55-

Gel electrophoresis(135 V, 25 min)


19:45

Took a picture



Transformation

  • Sample

Competent Cells : XL10G(K)

Plasmid : plux-GFP(Cm)


21:45-

CmのプレートにまいてCultured it at 37 degrees Celsius.



Digestion

  • Sample

Vector : plux-GFP(p15A)

Insert : sfGFP

Enzymes : Nco1, BamH1


21:17

We added Enzymes and took it 37 degrees Celsius.


---3 h---


We took it 65 degrees Celsius for 酵素を失活させる


---20 min---

24:42

We kept it in refrigerator.



PCR

  • Template

plux-HSUTK