Team:DTU Denmark/parts

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   Also check out our Danish website<br><br>
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   View all parts in registry<br><br>
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     <a href="http://www.bio.dtu.dk/igem.aspx" CLASS=leftbar>DTU iGEM 2009</a><br>
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     <a href="http://partsregistry.org" CLASS=leftbar>Parts registry</a><br>
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Revision as of 14:30, 3 October 2009

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cln2 PEST destabilization domain for rapid protein turnover

C-terminal domain of Saccharomyces cerevisiae cyclin 2 (CLN2) gene. It has been shown that this region of the protein, which is rich of PEST motifs, leads to a destabilization of the protein. Hence this Tag can be used to increase the turn-over rate of a protein. In the article by Mateus and Avery: "Destabilized green Fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry" they show that addition of these 178 carboxyl-terminal amino acid residues changes the half-life of a GFP from 7h and down to 30 minutes.

Registry id: BBa_K194000

GFP (a yeast- and FACS-optimized GFP variant)

Green Fluorescent Protein (GFP) codonoptimized for yeast.

Registry id: BBa_K194001

Destabilized GFP for yeast

This variant of biobrick BBa_K194001 has been destabilized by fusing biobrick BBa_K194000. In the article by Mateus and Avery: "Destabilized green Fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry" they show that addition of these 178 carboxyl-terminal amino acid residues of the Cln2 PEST signal, changes the half-life of a GFP from 7h and down to 30 minutes.

Registry id: BBa_K194002

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