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Team:Tsinghua/Result1
From 2009.igem.org
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Showed above are the maps of the GenSniper using bottom-up approach. The genes of Nu1, A, W, B and lysis genes (S, R, Rz) are incorporated into pET-28a, while C, D, E, FI and FII are synthesized into pACYCDuet-1. In other word, the GenSniper will have two linkage groups. | Showed above are the maps of the GenSniper using bottom-up approach. The genes of Nu1, A, W, B and lysis genes (S, R, Rz) are incorporated into pET-28a, while C, D, E, FI and FII are synthesized into pACYCDuet-1. In other word, the GenSniper will have two linkage groups. | ||
| - | [[Image:j21.png| | + | [[Image:j21.png|500px|center|j21]] |
| + | We have successfully cloned the Nu1-A, W-B, C and D-E-FI-FII gene segments from the bacteriophage lambda genome. | ||
| + | |||
[[Image:j22.png|600px|center|j22]] | [[Image:j22.png|600px|center|j22]] | ||
[[Image:j23.png|600px|center|j23]] | [[Image:j23.png|600px|center|j23]] | ||
Revision as of 04:49, 12 October 2009
Contents |
Synthesis of the Genome of GenSniper
Bottom-Up Approach
Showed above are the maps of the GenSniper using bottom-up approach. The genes of Nu1, A, W, B and lysis genes (S, R, Rz) are incorporated into pET-28a, while C, D, E, FI and FII are synthesized into pACYCDuet-1. In other word, the GenSniper will have two linkage groups.
We have successfully cloned the Nu1-A, W-B, C and D-E-FI-FII gene segments from the bacteriophage lambda genome.
Top-Down Approach
GenSniper Production Evaluation
