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Team:Tsinghua/Result2
From 2009.igem.org
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| + | This set of pictures show the expression of RFP. A shows the liquid of bacteria containing the Therapeutic DNA after 24h culture in LB. | ||
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Revision as of 07:21, 12 October 2009
The map of the Therapeutic DNA is listed above. It contains a incorporated cos site, O and P genes for replication and RFP expression segment. We should note that the RFP expression module in this Therapeutic DNA is downstream a prokaryotic promoter, which enable us to trace the function of the Therapeutic DNA according to red fluorescence. However, when applied to clinical use, the RFP expression segment can be replaced by the real therapeutic genes.
The above picture shows the purified Therapeutic DNA, which is actually a molecular cloning vector containing a cos site (can also be called as "cosmid").
This set of pictures show the expression of RFP. A shows the liquid of bacteria containing the Therapeutic DNA after 24h culture in LB.
PCR identification suggests that the therapeutic DNA does contain the cos site for the apckage into the GenSniper viroin.
