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Team:Queens/Notebook
From 2009.igem.org
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Laboratory One: Harry, Bogdan, James, Bryant Laboratory Two: Kate, Mike Tech Support and Laboratory Three: Parthiv, Chris, Chris |
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Laboratory One: Harry, Bogdan, James, Bryant 16/06/2009 -Lab Meeting -Finished the sequence for the surface expression construct -Finalized to-do list -Mini-prepped the following: -Linker K157013 Plate 3, Well 3G, plasmid Bba_K157000(A) -TEV Protease -I712078 (C-Terminus) Plate 2, Well 14M, plasmid J70003 (A) -I712077 (N-Terminus) Plate 2, Well 14K, plasmid J70003 (A) -pLux -R0062 (not leaky) Plate 1, Well 6O, plasmid pSB1A2 (A) -R1062 (median strength in the absence of luxR/HSL) Plate 1, Well 8G, plasmid pSB1A2 (A) -HO-pcyA -K098010 Plate 3, Well 11N, plasmid pSB3k3 (K) -Terminator -B0015 Plate 1, Well 23L, plasmid pSB1AK3 (AK) -RFP -E1010 Plate 1, Well 18F, plasmid pSB2K3 (K) |
