Team:KULeuven/Lab/Vanillin Production

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VANILLIN SYNTHESIS

Goal

Making vanillin from tyrosine in a five-step pathway.

Making vanillin from tyrosine

required

  • biobricks:
   SAM8: BBa_ I742142: coding sequence without RBS
   SAM5: no number: RBS site + PstI restriction site removed
   COMT: BBa_I742107: coding sequence without RBS
   FCS: BBa_I742115: RBS + fcs in pSB1A2 
   ECH: BBa_I742113: RBS + ech in pSB1A2

Where from

  • 1. COMT is available in 2009 spring distribution plate 1 well 19D

DNA is compatible

  • 2. SAM8, SAM5, COMT, FCS, ECH will be sent to us from the French Lab from the university Edinburgh