Team:UCL London/From the lab/Results

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Growth Cruves Experiments

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Note on Copper

Copper is an essential trace metal for all living organisms. Nevertheless, free intracellular copper is very toxic and has a negative impact on most cells even at very low concentrations. Even though the system for the control of copper levels not is fully understood in E.coli two systems, CueR and CusR, have been identified as regulatory systems for copper homeostasis. According to some researchers, increased levels of extra cellular copper may have the effect of denaturing some membrane proteins. (Yamamoto and Ishihama 2005) This has however not yet been proved so there is still a risk that the CpxAR system could be activated by some additional copper mediated mechanism independent of the presence of misfolded proteins in the periplasm.

Graphs

UCL Cu op 2h.png UCL Cu op night.png CuCl2graph.png CuSO4graph.png Irongraph.png

Growth Cruves Experiments

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Graphs

UCL Growth Curve Expt.pngFig.2a UCL TetR GC.pngFig.2b

UCL Fluorescent Bar Chart.png

UCL OD&F Control.png UCL OD&F RPU.png UCL OD&F degP.png UCL OD&F spy.png

Results

Fig.2a illustrated the growth curves of control (E.coli without any modified plasmids), RPU device ([http://partsregistry.org/Part:BBa_I20260 BBa_I20260]), degP+GFP device ([http://partsregistry.org/Part:BBa_BBa_K239015 BBa_BBa_K239015]), and spy device ([http://partsregistry.org/Part:BBa_BBa_K239009 BBa_K239009]). Fig.2b showed the growth curve of tetR+GFP device ([http://partsregistry.org/Part:BBa_K239012 BBa_K239012])

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