Team:BIOTEC Dresden/Notebook SilverNano
From 2009.igem.org
Silver Nano Particles
Strategy
Unique peptides have been found capable of nucleating metal atoms, spurred by an interest in biomineralization by the prospect of nanoscale building. We use a twelve amino-acid long silver binding peptide that has been identified by combinatorial screening to nucleate silver nanoparticles. The peptide is tagged with GFP so that it can be monitored along the flow chamber. Several protocols to bio-synthesize silver nanoparticles were tried in parallel, and absorbance of various samples were measured using spectrophotometry.
Here the basic ideas are introduced.
Lab notes and obtained results.