Virginia Commonwealth/23 July 2009

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Revision as of 17:25, 23 July 2009 by Trentay (Talk | contribs)

Contents

Thursday 23 July 2009

Results

Maria and Afton

  • All overnight cultures grew except one vial of NEB 10 beta seed stock
    • there was a second vial of seed stock that did grow successfully
  • Transformations were successful
Name Type Plate Purpose Growth Observation
NEB 10 beta seed stock LB make more seed stock lawn
NEB 10 beta electro comp. LB (+) Control/ make more stock lawn
NEB 10 beta electro comp. LB (+) Control shocked lawn
DB 3.1 electro comp. LB (+) Control shocked lawn
DB 3.1 electro comp. LB (+) Control/make more stock lawn
NEB 10 beta electro comp. Cm (-) Control no growth
NEB 10 beta electro comp. Cm J23100 w/ J06702 6 small colonies

Trentay 17:19, 23 July 2009 (UTC)


Tasks

Maria and Afton

  • Make cryogenic stocks of all overnight cultures
  • Make stocks of parts I1352 (RFP) and I13522 (GFP)
  • Update notebook, documents
  • Pick colonies from transformation
    • pick controls as well to make more seed and electrocompotent stock

Trentay 17:25, 23 July 2009 (UTC)


Wetlab

Maria and Afton

  • Cryogenic stocks were made

Trentay 17:25, 23 July 2009 (UTC)