Team:British Columbia/Project

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Home The Team The Project Parts Submitted to the Registry Modeling Sponsor Us! Calendar Biosensor Sensitivity Notebook Biosensor Logic Gate Notebook

Contents

Overall project

1. Sensitivity of a biosensor

2. Logic gates

Biosensor

Using a random mutagenesis method directed at the promoter binding sites, we intend to construct a library of varying strength pBAD promoters coupled to a GFP reporter gene. Sequencing of select mutated promoters will result in a collection of pBAD variants that cover a wide range promoter sensitivity.

The Experiments

1. Random mutagenesis of pBAD promoter sequence. 2. Quantification of mutant promoter-driven GFP fluorescence. 3. Sequencing of select mutants. 4. BioBrick design and submission.

Results

Logic Gates

Miscellaneous Data

Biobricks.zip - Fasta file containing every biobrick from [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=List Here]