August/10 August 2009

Revision as of 23:59, 10 August 2009 by Unocchi (Talk | contribs)

1.Min prep

1-6O   35.2 ng/uL 
1-18L  41.8 ng/uL 
1-10K  39.1 ng/uL

2.competent cells(with Ca)

3.digestion with a restriction enzyme

insert : E+S 
vector : E+x

4.Transformation

1-23L A 
1-18C A 
1-20F K 
1-12H K 
1-6I  A 
2-6P  A 
1-15N A