August/3 October 2009

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1.colony check

sample no.     no. of colony
   1-14N                0
   1-16I                 0
   1-14N                0 
   1-16I               +++
   2-18F              +++


2.transfromation

sample no.
59 , 58 . 60 , 61 , 62 , 63 , 64 , 65


K204066

VectorInsert
1-19B22-18F10
EcoRI1EcoRI1
XbaI1SpeI1
No.22No.22
dH2O14dH2O6
total20uLtotal20uL



37°C, 2hr
gel electorisis
gel cut & purification
ligation , O/N

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