August/7 October 2009

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1. colony check

sample no.     no. of colony
   11                   +++
   20                   +++
   21                   +++
  2-6O                 ++
  1-15J                +
  1-15L                +


2.transformation

sample no.
67 , 68 , 69 , 52 , 11 , 20 , 21 , 2-6O , 1-15J , 1-15L


3.digation

K204070

VectorInsert
332505
EcoRI1EcoRI1
XbaI1SpeI1
No.22No.22
dH2O12dH2O9
total20uLtotal20uL


K204071

VectorInsert
1-8K2514
EcoRI1EcoRI1
XbaI1SpeI1
No.22No.22
dH2O12dH2O10
total20uLtotal20uL

K204072

VectorInsert
2-8E2316
EcoRI1EcoRI1
XbaI1SpeI1
No.22No.22
dH2O12dH2O12
total20uLtotal20uL

K204073

VectorInsert
1-8I6326
EcoRI1EcoRI1
XbaI1SpeI1
No.22No.22
dH2O8dH2O8
total20uLtotal20uL


K204074

VectorInsert
1-1K101-12M2
SpeI1XbaI1
PstI1PstI1
No.2 2No.22
dH2O4dH2O12
total20uLtotal20uL


K204075

VectorInsert
1-1K101-8K2
SpeI1XbaI1
PstI1PstI1
No.2 2No.22
dH2O4/td>dH2O12
total20uLtotal20uL


37 degree . O/N

4. min prep

sample            conc.
 X4                   257 ng/uL
 X4                   266
 X4                   171
 X5                   169
 X6                   293
 X7                   294
 X8                   151
 59                   306
 61                   285
 64                   252
 65                   278


5.digation

X4

VectorInsert
32161-12M4
SpeI1XbaI1
PstI1PstI1
No.2 2No.22
dH2O2dH2O12
total20uLtotal20uL


37 degree , 2hr
gel cut


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